• Title/Summary/Keyword: pH decrease

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For the chlorophyll decrease and formation of Chlorolla variegata by Glucose-Culture (II) Influence of temperature and pH (Chlorella Variegata Beijerinck 의 Glucose 배양에 있어서의 Chlorophyll 증감관계에 관한연구 (제 2 ) .pH 의 영향에 대한)

  • 이현순
    • Korean Journal of Microbiology
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    • v.5 no.1
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    • pp.20-23
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    • 1967
  • I studied about temperature and pH concentration having influence upon chlorophyll decrease on the Glucose culture of Chlorella variegata Beijerinck(211/10a). I cultured under $25^{\circ}C$(standard), $15^{\circ}C$ and $35^{\circ}C$ and compared with each other. The culture of $15^{\circ}C$ didn't have any large difference with the standard culture($25^{\circ}C$) but the culture of $35^{\circ}C$ had a large amount of chlorophyll decrease without carbohydrate accumulation, stimulation of cell division and nitrogen-deficiency. Chlorella variegata had optimum pH 6.5-7 and was a little weak in all phenomenon under pH 8 rather than under pH 6.5-7. Under pH 5 they had deep chlorophyll decrease without phephytin.

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Survivals of Lactic Acid Bacteria and its Characteristics under the Acidic and Anaerobic Condition (혐기적 산성조건하에서 젖산균의 생존과 그 특성)

  • 신용서;김성효;이갑상
    • Microbiology and Biotechnology Letters
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    • v.23 no.4
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    • pp.373-377
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    • 1995
  • We investigated the survival, $\beta$-galactosidase activity and cellular permeability of lactic acid bacteria such as Lactobacillus acidophilus ATCC 4356, Lactobacillus casei subsp. casei IFO 3533, Streptococcus thermophilus KCTC 2185, Lactobacillus delbrueckii subsp. lactis ATCC 4797, and Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 in anaerobic condition of pH 1.5-3.5 range. Numbers of all tested viable cells did not decrease at pH 3.5, but decreased rapidly at pH 1.5 and pH 2.5 during 2 hour incubation at modified EG medium. Immediately after 2 hour incubation, the decrease in population at pH 1.5 and pH 2.5 was about 6-8 and 5-7 log cycles/ml, respectively. Lactobacillus acidophilus ATCC 4356 showed the higest survival of all tested bacteria. The $\beta$-galactosidase activity from Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 and Streptococcus thermophilus KCTC 2185 decreased rapidly at pH 1.5 and 2.5, but there was a little decrease at pH 3.5. The cellular permeability that was measured by the leakage of intracellular materials increased with decrease of pH. These results suggest that the ingested lactic acid bacteria may be destroyed in contact with low pH of gastric acid.

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A study on floating and sinking pulse by classification of pulse pattern through analysis of P-H volume-curve at 5 applied pressure levels (5단계 가압에 대한 맥파 변화 분석에 의한 맥 패턴 분류와 부침맥(浮沈脈) 연구)

  • Kown, Sun-Min;Kang, Hee-Jung;Yim, Yun-Kyoung;Lee, Yong-Heum
    • Korean Journal of Acupuncture
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    • v.27 no.1
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    • pp.13-22
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    • 2010
  • Objectives: The information on the depth where pulse wave appears is as important as pulse waveform. The aim of this study was to classify pulse pattern using pressure-height(P-H) volume-curve by 5 applied pressure levels to find out the information on the depth of pulse and interpret the floating & sinking pulse in oriental medical pulse diagnosis. Methods: We used 3 dimensional pulse imaging analyser (DMP-3000, DAEYOMEDI Co., Korea), which measures radial pulse waveforms noninvasively by way of tonometric method at 5 applied pressure levels, and shows P-H volume-curves by applied pressure. 448 subjects were enrolled, pulse waveforms were measured and the P-H volume-curves were gained on the three locations of Chon, Kwan, and Cheok. Results: Gained P-H volume curves were classified into 3 types ; increase type, decrease type, and increase-decrease type. Increase-decrease type appeared more often on Chon and Kwan, while increase type appeared more often on Cheok. In a few cases, decrease-type appeared on Chon and Kawn, however it never appeared on Cheok. Conclusions: Through the classification of pulse by P-H volume-curve, we gained the information on the depth of pulse. We speculate the decrease type as floating pulse, the increase-decrease type as middle pulse, and the increase type as sinking pulse in oriental medical pulse diagnosis. After more researches on P-H volume-curve by applied pressure, the P-H volume-curve may be used as an important factor for pulse diagnosis.

The Dyeability of Indigo Depending on the pH of Reduction Bath (환원액의 pH에 따른 인디고의 염색성)

  • 강지연;유효선
    • Textile Coloration and Finishing
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    • v.11 no.4
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    • pp.24-30
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    • 1999
  • Cotton fabric was dyed with indigo at different pHs, dye concentrations, and repeated dyeings. And the K/S value and dye uptake of the dyed materials at those conditions were determined. The decrease of pH during the reduction process of indigo stock solution was observed. As a result the decrease of pH of the dye stock solution depended on the dye concentration and initial pH. The highest K/S value was shown at fabric dyed at pH 11 and the relationship between K/S value and dye uptake depended on initial pH of reduction bath and the slope was lower as the pH increased to 13.

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Effect of pH in 3.5% NaCl aqueous solution and ferrite grain size on corrosion fatigue fracture of dual phase steel (複合組織鋼의 부식피로파괴에 미치는 3.5% NaCl水溶液의 pH와 母相粒徑의 效果)

  • 오세욱;강호민
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.11 no.6
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    • pp.867-876
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    • 1987
  • Corrosion fatigue fracture with change in the grain size of M.E.F. dual phase steel is investigated in 3.5% NaCI aqueous solution at pH 2, 4, 6, 9, and 11. Generally speaking, decrease in corrosion fatigue life is strongly dependent on decrease in pH and slightly on the grain size. For the B material with the big grain size, the fatigue life is small due to its large reduction ratio of corrosion fatigue life. The influence of grain size on the reduction ratio of corrosion fatigue life is large at pH 11-6. Whi9le at pH 4-2 the reduction ratio of corrosion fatigue life only depends on the corrosion effect. The larger grain size and the lass pH result in the greater influence on corrosion fatigue crack propagation rate. As pH decreases, the plateau portion in the crack propagation rate curves of the B material are distinct. Crack propagation rate curves become slow down at high .DELTA.K range because crack closure effect by minute corrosion products inside crack causes the oxidation corrosion action less effective for a certain period of time. In A material with small grain size, fatigue life is increased in proportion with increase of martensite intergranular which brings forth restraining the crack propagation decreases crack propagation rate. Corrosion pit which is created in the surface of specimen is found at pH 6,4 and 2 which is noticeable and the unevenness of the surface of the specimen becomes severe as pH decreases. The unevenness of corrosion fatigue fracture surface is severe as the effect of pH increases i.e. as pH decreases. In proportion with increase in the grain size and decrease in pH, the aspect of brittle fracture becomes evident.

Effects of Acidic Fermentation Products and Culture pH on the Maintenance Energy of Clostridium acetobutylicum (산성 발효 산물과 배양 pH가 Clostridium acetobutylicum의 유지 에너지에 미치는 영향)

  • 신순영;김병홍
    • Korean Journal of Microbiology
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    • v.28 no.3
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    • pp.268-273
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    • 1990
  • In order to elucidate the acid tolerance mechanism of Clostridium acetobutylicum against organic acid, the maintenance energy with added butyrate at different pH was determined. Maintenance coeffecient in acidogenic chemostat was higher at pH 6.5 than at pH 5.5 showing that this organism is an acidophile. The addition of butyrate at pH 5.5 and different dilution rate caused linear decrease of the cell concentration though $Y_{ATP}$ did not decrease with increasing undissociated organic acid. $Y_{ATP}$ decreased by increasing the concentration of undissociated organic acid at pH 5.0 by the addition of butyrate. From these results it is hypothesized that the ATP conaumption for pH stat of acidophile C. acetobutylicum is increased at the circumstance with over 30mM of undissociated organic acid.cid.

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Resistance to a pH Fall with Water-Cement Ratio in Cementitous Material (물-시멘트비에 따른 시멘트계 재료의 pH 저하 저항성 평가)

  • Jung, Min-Sun;Ann, Ki-Yong;Song, Ha-Won
    • Proceedings of the Korea Concrete Institute Conference
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    • 2009.05a
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    • pp.325-326
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    • 2009
  • The present study concerns the acid neutralization capacity (ANC) of cement paste, mortar and concrete depending on a free water-cement ratio. The ANC of paste at 30%, 40% and 50% of water-cement ratio was measured and simultaneously the effect of aggregate on the ANC was evaluated. It was found that an increase in the acid concentration resulted in a decrease in the pH of the suspension, in particular, at 10 in the pH, a sharp decrease was observed. The ANC showed some peak resistances to acid at particular pH values.

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Response of Syntrophic Propionate Degradation to pH Decrease and Microbial Community Shifts in an UASB Reactor

  • Zhang, Liguo;Ban, Qiaoying;Li, Jianzheng;Jha, Ajay Kumar
    • Journal of Microbiology and Biotechnology
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    • v.26 no.8
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    • pp.1409-1419
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    • 2016
  • The effect of pH on propionate degradation in an upflow anaerobic sludge blanket (UASB) reactor containing propionate as a sole carbon source was studied. Under influent propionate of 2,000 mg/l and 35℃, propionate removal at pH 7.5-6.8 was above 93.6%. Propionate conversion was significantly inhibited with stepwise pH decrease from pH 6.8 to 6.5, 6.0, 5.5, 5.0, 4.5, and then to 4.0. After long-term operation, the propionate removal at pH 6.5-4.5 maintained an efficiency of 88.5%-70.1%, whereas propionate was hardly decomposed at pH 4.0. Microbial composition analysis showed that propionate-oxidizing bacteria from the genera Pelotomaculum and Smithella likely existed in this system. They were significantly reduced at pH ≤5.5. The methanogens in this UASB reactor belonged to four genera: Methanobacterium, Methanospirillum, Methanofollis, and Methanosaeta. Most detectable hydrogenotrophic methanogens were able to grow at low pH conditions (pH 6.0-4.0), but the acetotrophic methanogens were reduced as pH decreased. These results indicated that propionate-oxidizing bacteria and acetotrophic methanogens were more sensitive to low pH (5.5-4.0) than hydrogenotrophic methanogens.

Improving Coagulation Performance with pH Preadjustment in Drinking Water Treatment (정수처리에서 pH 저감에 의한 응집효율향상에 관한 연구)

  • Lee, Hwan;Lee, Cheol-Hyo;Jung, Chang-Gue;Lee, Yoon-Jin
    • Journal of Environmental Health Sciences
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    • v.29 no.2
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    • pp.1-6
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    • 2003
  • This paper reports on a pilot scale comparison of PACS coagulation with and without pH preadjustment. The pH of the water was adjusted with carbon dioxide and sulfuric acid. Process performance was assessed on the basis of total organic carbon(TOC), UV absorbance, turbidity and disinfection by-product(DBP) precursors. Coagulation pH appeared to be a determining factor for maximum NOM removal. The optimum coagulation pH in order to decrease TOC and turbidity were pH 7. Preadjustment of pH 7 increased TOC removal to as much as 43, 47 percent with sulfuric acid and carbon dioxide. Moreover, coagulation at pH 7 caused a reduction in UV$_{254}$, THMFP and HAAFP compared to the baseline coagulation. For preadjustment of pH 7 with carbon dioxide, the percentage of TOC, UV$_{254}$, THMFP and HAAFP shows the reduction rate of 3.8, 0.5, 4.8, 9.4% comparing to the coagulation rendition using sulfuric acid. Acid addition to depress pH during coagulation decrease Langelier Saturation Index(LSI), potentially causing increase corrosion in water distribution systems. LSI for carbon dioxide and sulfuric acid at pH 6 was -2.3, -3.3. Therefore, carbon dioxide was more effective at controlling corrosion than sulfuric acid.

Growth and Physiology of Thiobacillus novellus under Autotrophic and Heterotrophic Conditions (자가영양과 타가영양 조건하에서 Thiobacillus novellus의 생리 및 성장)

  • 박인국
    • Korean Journal of Microbiology
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    • v.29 no.4
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    • pp.263-266
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    • 1991
  • The growth of T. novellus in auto trophic and geterotrophic media was studied to determine the time required for cells to enter stationary phase and relative percentage of ribosomal proteins. When T. novellus was grown autotrophically, growth proceeded at a slow rate characteristic of autotrophs and did not enter log phase until the end of the first day. Logarithmic growth proceeded for 3-4 days at which time the cells entered the stationary phase. In particular, logarithmic growth was accompanied by decreasing pH of culture media and in the stationary phase the pH levelled off at 6.0, a decrease of 1.6 pH value compared to original pH of media. The pH decrease was greatest during log phase when cells oxidized thiosulfate to $H_{2}$$SO_{4}$. The doubling time was about 26h. In heterotrophic media growth proceeded at a much faster rate and cells entered stationary phase 20-22h after inoculation. The doubling time was 3h. The protein content of the ribosomes in T. novellus grown heterotrophically was 4.2% greater than those from the organism grown autotrophically.

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