• 전력전자학회:학술대회논문집
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• 전력전자학회 2003년도 춘계전력전자학술대회 논문집(1)
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• pp.27-30
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• 2003

This paper describes two novel current fed high frequency resonant inverter can be used as the power supp]y for wax-sealing. This two topology can be obtained higher output frequency than switching frequency by composing modified unit inverter based on conventional half-bridge serial resonant inverter in parallel with input power source. also, By using time-sharing gate control method, this proposed inverter can not only realize the output control of dependence irrespective of the switching frequency using phase-shift but also reduce switching loss because it has ZVS function. Simulation results through the Pspice have demonstrated the feasibility of the proposed inverter. This proposed inverter will be able to be practically used as a power supply in various fields as induction heating applications, DC-DC converter etc.

• Progress in Superconductivity
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• 제6권1호
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• pp.89-94
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• 2004

The policrystalline$SmBa_2$$Cu_2$$O_{y}$ was synthesized by the solid state reaction method. The dependence of AC susceptibility on temperature and applied ac field was studied. The critical temperature $T_{c}$ is about 92 K. As the ac field is increased, the slope and the value of real part of susceptibility become smaller and the peak position of imaginary part $T_{P}$ was shifted to a lower temperature with peak broadening. Using Bean's model, we determined intergrain critical current density $J_{c}$ and obtained $44 A/{cm}^2$ at 75 K. From the relation of the $J_{c}$ (T)=($1-T/T_{c}$ )$^{\beta}$ we obtained $\beta$=0.8 and found that the Josephson junction type of the $SmBa_2$$Cu_2$$O_{y}$ is SIS junction. The peak of the imaginary part shifts to higher temperature with increasing frequency, f. from Arrhenius plot, we obtained the activation energy of about 0.96 eV.

• BMB Reports
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• 제40권6호
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• pp.1009-1015
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• 2007

In this communication, we report the efficacy of $\beta$-carotene towards differentiation and apoptosis of leukemia cells. Dose ($20{\mu}M$) and time dependence (12 h) tests of $\beta$-carotene showed a higher magnitude of decrease (significance p < 0.05) in cell numbers and cell viability in HL-60 cells than U937 cells but not normal cell like Peripheral blood mononuclear cell (PBMC). Microscopical observation of $\beta$-carotene treated cells showed a distinct pattern of morphological abnormalities with inclusion of apoptotic bodies in both leukemia cell lines. When cells were treated with $20{\mu}M$ of $\beta$-carotene, total genomic DNA showed a fragmentation pattern and this pattern was clear in HL-60 than U937 cells. Both the cell lines, on treatment with $\beta$-carotene, showed a clear shift in $G_1$ phase of the cell cycle. In addition the study also revealed anti-oxidant properties of $\beta$-carotene since there was reduction in relative fluorescent when treated than the control at lower concentration. Collectively this study shows the dual phenomenon of apoptosis and differentiation of leukemia cells on treatment with $\beta$-carotene.

• 한국수산과학회지
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• 제51권5호
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• pp.590-594
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• 2018

We developed a time-temperature indicator (TTI) that rapidly determines whether frozen mackerel Scomber japonicus has thawed during its distribution. Our TTI was made from filter paper capable of absorbing mackerel exudate that was soaked in a 20% citric acid solution at pH 4.0 and then dried. The dried absorbent paper was cut and attached to blue litmus paper with a 2 mm overhang. The fixed litmus paper was covered with a polypropylene film and sealed. The indicator was placed inside a polyvinyl vacuum package containing the mackerel sample. The vacuum-sealed packaged was frozen at $-20^{\circ}C$ for 24 h. After freezing, the color change and time dependence of the indicator were observed at room temperature ($25^{\circ}C$) and demonstrated the utility of this TTI for rapidly determining whether frozen mackerel underwent thawing during distribution.

• 대한화학회지
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• 제36권4호
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• pp.517-522
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• 1992

10$^{\circ}C$와 20$^{\circ}C$에서 ${\beta}$-피콜린과 치환된 벤조일클로라이드류의 반응을 압력변화에 따라 아세토니트릴용매내에서 전기전도도법으로 연구하였다. 유사 1차속도상수와 2차속도상수로부터 여러 가지 활성화파라미터들(${\Delta}V{\neq}$, ${\Delta}{\beta}{\neq}$, ${\Delta}H{\neq}$, ${\Delta}S{\neq}$ and ${\Delta}G{\neq}$)을 구하고, Hammett ${\rho}$값의 압력의존성을 설명하였다. ${\Delta}V{\neq}$, ${\Delta}{\beta}{\neq}$ 및 ${\Delta}S{\neq}$ 는 모두 음의 값을 나타내었고, 압력증가에 따라 속도상수와 Hammett ${\rho}$값은 증가하며, 결합형성이 더욱 촉진된 반응메카니즘으로 진행됨을 알 수 있었다.

• Journal of Magnetics
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• 제16권1호
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• pp.15-18
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• 2011

We have studied the $TiO_2$ doping effects on the flux pinning behavior of an $MgB_2$ superconductor synthesized by the in-situ solid-state reaction. From the field-cooled and zero-field-cooled temperature dependences of magnetization, the reversible-irreversible transition of $TiO_2$-doped $MgB_2$ was determined in the H-T diagram (the temperature dependence of upper critical magnetic field and irreversibility line). For comparison, the similar measurements are also obtained from SiC-doped $MgB_2$. The critical current density was estimated from the width of hysteresis loops in the framework of Bean's model at different temperatures. The obtained results manifest that nano-scale $TiO_2$ inclusions served as effective pinning centers and lead to the enhanced upper critical field and critical current density. It was concluded that the grain boundary pinning mechanism was realized in a $TiO_2$-doped $MgB_2$ superconductor.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2000년도 추계학술대회 논문집
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• pp.29-32
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• 2000

The static and metadynamic recrystallization of non-heat treated medium carbon steel(Fe - 0.45wt.$\%C\;-\;0.6wt.\%Si\;-\;1.2wt.\%Mn\;-\;-0.12wt.\%Cr \;-\;0.1wt.\%V \;-\;0.017wt\%$.Ti) were studied by the torsion test in the strain rate range of 0.05 - 5 $sec^{-1}$, and in the temperature range of $900\;-\;1100\;^{\circ}C$. Interrupted deformation was performed with 2 pass deformation in the pass strain range of $0.25 {\varepsilon}_p(peak strain)\;and\;{\varepsilon}_p$, and in the interpass time range or 0.5 - 100 sec. The dependence or pass strain(${\varepsilon}_i$), strain rate( $\dot{\varepsilon}$ ), temperature(T), and interpass time($t_i$) on static recrystallization (SRX) and metadynamic recrystallization (MDRX) were predicted from the modified Avrami's equations respectively. Comparison of the softening kinetics between SRX and MDRX was indicated that the rate of MDRX was more rapid than that of SRX under the same deformation variables.

• BMB Reports
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• 제35권2호
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• pp.228-235
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• 2002

Methionine aminopeptidase (MetAP) catalyzes the removal of an amino-terminal methionine from a newly synthesized polypeptide. The enzyme was purified to homogeneity from Bacillus stearothermophilus (KCTC 1752) by a procedure that involves heat precipitation and four sequential chromatographs (including DEAE-Sepharose ion exchange, hydroxylapatite, Ultrogel AcA 54 gel filtration, and Reactive red 120 dye affinity chromatography). The apparent molecular masses of the enzyme were 81,300 Da and 41,000 Da, as determined by gel filtration chromatography and sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE), respectively. This indicates that the enzyme is comprised of two identical subunits. The MetAP specifically hydrolyzed the N-terminal residue of Met-Ala-Ser that was used as a substrate, and exhibited a strong preference for Met-Ala-Ser over Leu-Gly-Gly, Leu-Ser-Phe, and Leu-Leu-Tyr. The enzyme has an optimal pH at 8.0, an optimal temperature at $80^{\circ}C$, and pI at 4.1. The enzyme was heat-stable, as its activity remained unaltered when incubated at $80^{\circ}C$ for 45 min. The Km and Vmax values of the enzyme were 3.0mM and 1.7 mmol/min/mg, respectively. The B. stearothernmophilus MetAP was completely inactivated by EDTA and required $Co^{2+}$ ion(s) for activation, suggesting the metal dependence of this enzyme.

• 대한화학회지
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• 제42권4호
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• pp.422-431
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• 1998

Salicyladehyde와 2-hydroxy-1-naphthaldehyde를 2-aminophenol과 2-amino-p-cresol에 반응시켜 세자리 Schiff base 리간드$(SIPH_2,\;SIPCH_2,\;HNIPH_2,\; HNIPCH_2)$들을 합성하였으며 이들 리간드를 Co(II) 이온과 반응시켜 세자리 Schiff base Co(II) 착물들을 합성하였다. 리간드와 착물들의 구조와 특성을 원소분석, $^1H$-NMR, IR, UV-vis 분광법과 열 무게 분석법으로 예측하였다. Co(II) 착물들은 Schiff base 리간드와 금속(II)의 몰비가 1:1로 결합하였으며, 3분자의 수화물이 배위된 6배위 착물 구조임을 알았다.지지 전해질로서 0.1M TBAP를 포함한 DMSO 용액에서 순환 전압전류법으로 세자리 Schiff base 리간드와 이들의 Co(II) 착물들의 전기 화학적인 산화·환원 과정을 알아보았다. 리간드들의 전기 화학적 환원은 확산 지배적이고 비가역적으로 진행되었으며 Co(II) 착물의 전기 화학적 환원과정은 2단계의 1전자 반응으로 확산 지배적이고 비가역적으로 진행되었다. Co(II) 착물들의 환원전위는 [Co(II)$(HNIPC)(H_2O)_3$]>[Co(II)$(HNIP)(H_2O)_3$]>[Co(II)$(SIPC)(H_2O)_3$]>[Co(II)$(SIP)(H_2O)_3] 순으로 약간 양전위 방향으로 이동하였으나 리간드의 영향은 크게 받지 않았다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7197-7201
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• 2013

Aim: To investigate the effects of diallyl trisulfide (DT) on apoptosis of cisplatin (DDP)-resistant human epithelial ovarian cancer SKOV-3 cells (SKOV-3/DDP), and the role of p53 upregulated modulator of apoptosis (PUMA). Methods: SKOV-3/DDP cells were randomly divided into control, DT, DPP and DPP+DT groups, which were treated with DT or combined DT and DDP. All cells were incubated for 48 h. and apoptosis rates were assessed by flow cytometry. mRNA and protein expression of PUMA, Bax and Bcl-2 was determined by RT-PCR and Western blot assays, respectively. Results: Compared with control group, the apoptosis rates of SKOV-3/DDP cells in DT groups were obviously increased, with dose-dependence (P < 0.05), the mRNA and protein expressions of PUMA, Bax also being up-regulated (P < 0.05), while those of Bcl-2 were down-regulated (P < 0.05). Compared with DT groups, the apoptosis rate in the DDP+DT group was significantly increased (P < 0.05). After knockdown of PUMA with specific siRNA, the apoptosis rate of SKOV-3/DDP cells was obviously decreased (P < 0.05). Conclusion: DT can promote the apoptosis of SKOV-3/DDP cells with PUMA playing a critical role.