• Title/Summary/Keyword: pH 측정 시험

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Studies on the Purifiction of Newcastle Disease Virus by Agar Gel Filtration (한천(寒天) 겔 여과법(濾過法)에 의한 뉴켓슬병(病)바이러스의 순화시험(純化試驗))

  • Kim, Sun Joong;Jeon, Yun Seong
    • Korean Journal of Veterinary Research
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    • v.8 no.1
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    • pp.6-10
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    • 1968
  • 순화(純化)된 뉴켓슬병(病) 바이러스를 얻기 위(爲)한 기초적(基礎的)인 실험(實驗)으로 바이러스 재료(材料)를 완형액(緩衡液)의 종류(種類) 및 수소(水素)이온 농도(濃度), 한천농도(寒天濃度)를 달리한 한천(寒天)겔칼럼에 통과(通過)시켰다. 그러고 바이러스 재료(材料)의 혈구응집력가(血球凝集力價)와 총질소량(總窒素量)을 비교(比較) 측정(測定)하여 다음과 같은 결과(結果)를 얻었다. 1. 한천(寒天)겔 응과법(凝過法)을 뉴켓슬병(病) 바이러스의 순화(純化)에 간단(簡單)히 이용(利用) 할수 있다. 2. pH 7.0 이상(以上)의 인산식염(燐酸食鹽) 완형액(緩衡液)과 5~8%의 한천농도(寒天濃度) 입자(粒子)를 이용(使用)할때 가장 좋은 결과(結果)를 얻을 수 있었다. 3. 매시(每時) 5~10ml의 유출속도(流出速度)일때 55ml 부터 80ml의 유출액중(流出液中)에서 고농도(高濃度)의 바이러스를 얻을 수 있었다.

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Prediction of Carcass Yield by Ultrasound in Hanwoo (초음파 측정에 의한 한우의 도체육량 예측)

  • Rhee, Y. J.;Jeon, K. J.;Choi, S. B.;Seok, H. K.;Kim, S. J.;Lee, S. K.;Song, Y. H.
    • Journal of Animal Science and Technology
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    • v.45 no.2
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    • pp.335-342
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    • 2003
  • This study was conducted to predict the carcass yield traits using ultrasound before slaughter and to enhance the prediction accuracy of carcass yield grade by applying various strategies. For this experiment, five hundred seventy three Hanwoo steers of 24 months of age were used. Difference between ultrasound result and carcass measure of BFT and LMA was 0.6$\pm$1.65mm and 0.7$\pm$5.56cm2, respectively. Correlation coefficient between ultrasound result and carcass measure of BFT and LMA was 0.86 and 0.82, respectively (p<0.001). Results for improving predictions of yield grade by four methods-the Korean yield grade index equation, fat depth alone, regression and decision tree methods were 80.3%, 81.3%, 80.1% and 81.8%, respectively. We conclude that the decision tree method can easily predict yield grade and is also useful for increasing prediction accuracy rate.

Effects of Property Transformation and Marginal Gap Depending on Heat-Treatment of Metal-Porcelain by Gold Electro-Forming System (전기성형법을 이용한 치과용 금속-도재관의 소성에 따른 물성 변화가 변연 간격에 미치는 영향)

  • Kim, Chi-Young;Chung, In-Sung;Choi, Sung-Min
    • The Journal of the Korea Contents Association
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    • v.10 no.6
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    • pp.352-359
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    • 2010
  • The objective of this paper is to study on the property transformation for materials and to evaluate the precision through marginal gap depending on heat-treatment of metal-porcelain by the gold electro-forming system. Specimens for evaluating property of hardness treat non-treat(HC), once(H1), twice(H2), and three times(H3) by heating, respectively. Specimens for marginal gap classified as each groups of special bonder(FS), special bonder, opaque(FO), special bonder, opaque, body(FB) and non-treatment(FC). As a result for micro-hardness test, the level of hardness on each groups of H1, H2, H3 decreased rather than HC group(p<0.05). In addition, marginal gap was not affected by the number of times being treated by heating. Therefore, the marginal fitness and the precision for mterials were not affected by the heat-treatment on the process to make GES metal-porcelain.

Development of analytical method for cyantraniliprole residues in welsh onion (Allium species) (대파(Allium속)에서 살충제 Cyantraniliprole 잔류분석을 위한 시험법 개발)

  • Do, Jung-Ah;Lee, Mi-Young;Chang, Moon-Ik;Hong, Jin-Hwan;Oh, Jae-Ho
    • Analytical Science and Technology
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    • v.28 no.3
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    • pp.175-181
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    • 2015
  • Cyantraniliprole, which is an ananthranilic diamide insecticide that was developed by the DuPont Corporation, was registered in the Republic of Korea in 2012. It offers exceptional insecticidal activity on a broad range of Lepidopera, Coleoptera, Diptera, and Isoptera. The maximum residue limits are set to pepper, peach, apple, sweet pepper, welsh onion, and so on (0.2~2.0 mg/kg). Therefore, an analytical method for determining cyantraniliprole residue in agricultural products was developed to ensure food safety. In previous studies, welsh onions were among vegetables included in the allium species, which is a representative plant with sulfur organic compounds. In this study, the analytical method was developed and evaluated for the elimination of sulfur compounds from the test solution of allium species during pesticide residue analysis. In order to inactivate the enzyme allinase and produce sulfur compounds, sample extraction was made in the base state pH 10 by reducing the activity of the enzyme. The recoveries of the developed method ranged from 81.9% to 83.2%, and the relative standard deviations were less than 10%. Therefore, based on the results, the method developed in this study is accurate and appropriate for use in cyantraniliprole determination. It will be used as the official method for managing the safety of cyantraniliprole residues in agricultural products.

11S and 7S Globulin Fractions in Soybean Seed and Soycurd Characteristics (콩 종실 단백질 분획(7S, 11S)과 두부특성)

  • Kim, Yong-Ho;Kim, Seok-Dong;Hong, Eun-Hi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.4
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    • pp.348-352
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    • 1994
  • Soybean seed consists of two major storage protein, the 7S and 11S globulins. For improving the quality of soybean seed protein, an increase of 11S/7S ratio would be a desirable objective because the 11S globulin contains much more the sulfur-containing amino acids than the 7S globulin. In this study, some soybean varieties were used to investigate the analyzing method for 7S and 11S globulins. 7S and 11S globulins couble be fractionated by their different solubilities in tris buffers. Adjusting the pH and tris concentration were major factors affecting the precipitation of the two globulins. And it was possible to screen the soybean genotypes having aberrant subunit compositions of the two globulins by an sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of total soybean proteins. The ratio of 11S to 7S globulin ranged from 1.29 to 1.38. This paper also dealed with the contribution of protein components in soybean seeds to the physical properties of soycurd. It indicated that the soycurd from crude 11S was remarkably harder than that from crude 7S, and springiness and cohesiveness were slightly higher in soycurd having higher proportion of 11S. So, it may concluded that proportion of protein components in soybean seed can be important factor which controls the suitability for soycurd or other foods.

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Effects of Insoluble Dietary Fiber Supplementation on Performance and Nutrient Digestibility of Weanling Pigs (난용성 식이섬유의 첨가가 이유자돈의 생산성 및 영양소 소화율에 미치는 영향)

  • Han, Y.K.;Han, K.Y.;Lee, J.H.
    • Journal of Animal Science and Technology
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    • v.47 no.4
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    • pp.565-572
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    • 2005
  • An experiment was conducted to determine the effect of supplementation of insoluble dietary fiber (Vitacel®) on growth performance and nutrient digestibility in weanling pigs. A total of 96 pigs that averaged 6.49$\pm$0.52kg BW and 23$\pm$2.1d age were allocated in a randomized block design with two pigs per pen and 12 pens per treatment. Pigs and feeders were weighed 10-days interval for the 40-d trials to determine ADG, ADFI and feed:gain ratio(F:G). Pigs were fed one of four diets:1) Control diet (C) 2) C+0.3% insoluble dietary fiber(IDF) 3) C+0.6% IDF and 4) c+0.9% IDF. For the determination of fecal nutrients digestibility, pigs were fed diets(diet 2) with 1% Celite-545(Fluka) as a marker and feces were collected on $9^{th}$ day and $18^{th}$ day after feeding diet 2. During the whole experimental period, pigs fed diet with 0.3% IDF have significantly higher ADG than other dietary treatment groups(P<0.05). ADG of pigs fed diet with 0.6% IDF was higher than that of pigs fed control diet(P<0.05). However, there was no significant difference in ADG between control group and 0.9% IDF group(P>0.05). ADFI of pigs fed diet with 0.3% IDF was significantly higher than any other dietary treatment groups(P<0.05). There was no significant difference in ADFI among control group, 0.6% and 0.9% IDF supplementation groups (P>0.05). Digestibilities of organic matter, crude protein, crude fiber and energy were significantly higher in 0.3% IDF supplementation group than any other dietary groups. However, there was no difference in over all nutrient digestibilities between 0.6% and 0.9% IDF group. Feeding diets more than 0.6% IDF did not affect the rest of the nutrients digestibilities except for ADF digestibility compared to control diet. Dietary supplementation level of IDF showed a significant quadratic effect on performance improvement of piglets. This response of growth performance to IDF supplementation is, as expected, in agreement with that of nutrient digestibility. Our results showed that IDF supplementation to diet for weaned piglets might be beneficial in terms of growth and nutrient digestibility. However, there should be more study on the relationship between level of IDF supplementation and piglet response as well as the exact mode of action of IDF in weaned piglets.

A Comparative Study on the Chemical Methods for the Determination of Available Phosphorus in Korean Soils (한국토양(韓國土壤)의 유효인산량(有效燐酸量) 검정(檢定)을 위한 화학적(化學的) 방법(方法)에 대한 연구(硏究))

  • Lim, Sun-Uk;Chung, Jong-Bae;Sa, Tong-Min
    • Applied Biological Chemistry
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    • v.29 no.1
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    • pp.62-72
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    • 1986
  • At present, the definition and chemical analysis method of available soil phosphorus for plants have not been standardized because of the complexity of crop and soil characteristics in Korea and many analysis methods have been suggested with different extraction conditions. Suitable analytical method of available soil P should be established by the trial of various methods based on crop nutrition and soil conditions. To establish the most suitable analysis method of available soiIP, a pot experiment with young maize was conducted over 44 different upland soils collected over the land of Korea. The amount of uptaken P by the plant was determined by ten different chemical methods for the available soil P. The results obtained were as follows: 1. Total phosphorus content in the sample soils ranged ranged $533{\sim}4917\;ppm$, and showed significant positive correlation with the content of organic matter. 2. The P content was relatively low in the acid sulfate soil and very high in the volcanic ash soil although both types of soil contained high level of orgic matter. 3. The amount of extractable P determined by ten different methods were varied more or less, and the ratios of the extractable P to the total soil P were in the range of $1{\sim}48%$. 4. The relative values to the amount of extractable soil P by different methods were in the order of $H_2O(5\;min.)\;1.0\;<\;H_2O(60min.)\;2.27\;<\;NH_4HCO_3\;5.57\;<\;NaHCO_3\;7.42\;<\;Double\;lactate\;9.71\;<\;Bray\;No.1\;12.53\;<\;Lancaster\;17.63\;<\;Nelson\;25.96\;<\;AcOH\;27.6\;<\;CAL-method\;50.27$ 5. The amount of extractable P determined by all of applied methods was very low in acid sulfate soil, volcanic ash soil and coarse textured soil. 6. Soil pH and total soil P generally showed significant positive correlation with the chemically extracted P, and soil organic matter was negatively correlated with the determined by Nelson-and CAL-method. Olsen method which showed significant correlation with exchangeable calcium seemed to be recommendable for calcareous soils. 7. Total amount of uptaken P by Young maize through continuos twice cropping was 4.05% of total soil P in average, and the uptake in the second cropping was twice as much as that of the first cropping. 8. Three determination methods, i.e. Soltanpour-, Double lactate and Bray No. 1-method seemed to be more suitable than Lancaster method which is widely practiced at present in Korea. However, further study should be carried out with other crops and soils to most adequate chemical method for determination of available soil P.

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Actual Uses and Water Qualities of Irrigation Water from Agricultural Reservoir and Pumping Station (관개용 저수지 및 양수장의 농업용수 공급량 및 수질)

  • Choi, Jin-Kyu;Han, Kang-Wan;Koo, Ja-Woong;Son, Jae-Gwon
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.3
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    • pp.205-212
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    • 2001
  • Joongpyong reservoir and Guiseok pumping station were selected as the hydrologic monitoring sites for the water level gauging and amount of irrigation water use, and water samples were taken and analysed periodically. Rating curves were derived from the relations water level and discharge data, and water supply through culvert of the Joongpyong reservoir was $593,200m^3$ and irrigation water $2,913,000m^3$ from the Guiseok pumping station. Changes of stream water qualities such as water temperature, pH, EC, total nitrogen, total phosphorus were examined, pH was 6.98.1 lower than the criteria of agricultural water 6.0~8.5, total nitrogen $0.9{\sim}4.5mg\;L^{-1}$, total phosphorus $0.008{\sim}0.036mg\;L^{-1}$. The results from this study could be offered as a basic data for the irrigation plan and water quality management.

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Heat Penetration Characteristics and Keeping Quality of Retort Pouched Curry (레토르트 파우치 카레의 전열특성 및 품질안정성)

  • Koo, Bon-Youl;Park, Seong-Joon;Byeon, You-Ryang;Son, Se-Hyeong
    • Korean Journal of Food Science and Technology
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    • v.25 no.1
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    • pp.63-68
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    • 1993
  • Heat penetration characteristics of retort pouched curry were studied to determine optimal sterilization conditions. Heating curve of retort pouched curry showed a simple logarithmic curve. The $f_h$ value (min.) of solid part in retort pouched curry was 1 min. higher than that of liquid part. $f_h$ value (min.) and $j_h$ value (heating lag factor) ranged from 8.3 min. to 12 min. and 1.0 to 1.17 respectively with increasing the ratio of solid to liquid and viscosity. $f_h$ value (min.) and $j_h$ value were significantly increased from 7.4 min. to 12.6 min. and 1.0 to 1.14 respectively with increasing thickness of the pouch from 11 to 15 mm. The low and medium volatile aroma components of retort pouched curry decreased during sterilization, which increased the high volatile aroma components. The retort pouched curry processed at $120^{\circ}C$, for 24 min. with $F_o$, value of $8{\sim}9$ could keep up a desirable quality without any remarkable deterioration.

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A study on determination of the lime requirement based on exchangeable aluminum content (치환성(置換性) Al 함량(含量)에 따른 석탄소요량(石炭所要量) 결정(決定)에 관(關)한 연구(硏究))

  • Ryu, In Soo;Cho, Seong Jin;Yuk, Chang Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.7 no.3
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    • pp.185-191
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    • 1974
  • Incubation and pot studies were conducted with upland soils for a study on determination of the lime requirement based on exchangeable alumium content. The results obtained are as follows; 1. Results of chemical analysis of upland soils show that pH varies from 5.0 to 5.4, and exchangeable Al moves with the range of 1.3-3.0m.e/100gr. Exchangeable Al decreases with years of cultivation. 2. Incubation studies shows that on acid mineral soils almost all exchangeable Al, on average 95% was neutralized with the lime to neutralized 100% exchangeable Al. On volcanic ash soil, however, only 65.5% was neutralized with the lime estimated to neutralize the equivalent of 200% exchangeable Al. The latter has required more lime. 3. The pH of mineral soils is on the average increased from an initial 5.2 to 6.3 when 95% of exchangeable Al is neutralized, whereas that on volcanic ash soil is increased from an initial 5.3 to 5.5 only when lime is applied at rate to neutralize the equivalent of 200% exchangeable Al. 4. A high correlation coefficient (r=0.99) was obtained between exchangeable Al and exchangeable acidity. This indicates that exchangeable acidity is primarly a result of exchangeable Al. 5. In pot experiments with soybean cultivated on one of the hill land soils (Songjoong soil) the application of fused phosphate and triple superphosphate based on a 5% saturation rate ($P_2O_5$ 32.1 kg/10a) showed that the liming factor for calculation of the optimum lime requirements based on exchangeable acidity was 0.594 for fuses phosphate or 1.132 for tripple superphosphate, and optimum pH is approximately 6.0 and optimum neutralization rate of exchangeable Al is 80-90%.

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