• BMB Reports
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• 제38권1호
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• pp.65-70
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• 2005

Choristoneura fumiferana granulovirus (ChfuGV) infection results two types of enveloped virions: Occlusion-derived virus (ODV) and budded virus (BV). Structural proteins ODVP-6E/ODV-E56 and p74 are two major conserved ODV-associated proteins that may be involved in the initiation of viral infection cycle in susceptible host insect larvae. This study presents the characterization of ChfuGV odvp-6e/odv-e56 and p74 transcription and translation as well as immunolocalization of these proteins in the occluded ChfuGV virion. Our results revealed that the transcription of odvp-6e/odv-e56 and p74 genes, both, start at 24 hours post infection (h p.i.). Using monospecific polyclonal antibodies made against ODVP-6E/ODV-E56 and p74 we demonstrated that these proteins are both expressed late in infection (24 h p.i.). Immunogold labeling using antisera against ODVP-6E/ODV-E56 and p74 proteins demonstrated that ODVP-6E/ODV-E56 and p74 proteins are both associated with the ODV envelop of ChfuGV.

• BMB Reports
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• 제36권5호
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• pp.475-487
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• 2003

A gene that encodes a homologue to baculoviral p74, an envelope-associated viral structural protein, has been identified and sequenced on the genome of Choristoneura fumiferana granulovirus (ChfuGV). A part of the ChfuGV p74 gene was located on an 8.9 kb BamHI subgenomic fragment using different sets of degenerated primers. These were designed using the results of the protein sequencing of a major 74 kDa structural protein that is associated with the occlusion-derived virus (ODV). The gene has a 1992 nucleotide (nt) open-reading frame (ORF) that encodes a protein with 663 amino acids with a predicted molecular mass of 74,812 Da. Comparative studies revealed the presence of two major conserved regions in the ChfuGV p74 protein. This study also shows that all of the p74 proteins contain two putative transmembrane domains at their C-terminal segments. At the nucleotide sequence level, two late promoter motifs (TAAG and GTAAG) were located upstream of the first ATG of the p74 gene. The gene contained a canonical poly(A) signal, AATAAA, at its 3' non-translated region. A phylogenetic tree for baculoviral p74 was constructed using a maximum parsimony analysis. The phylogenetic estimation demonstrated that ChfuGV p74 is related the closest to those of Cydia pomonella granulovirus (CpGV) and Phthorimaea operculella granulovirus (PhopGV).

• 한국미생물·생명공학회지
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• 제1권1호
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• pp.37-42
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• 1973

본실험에서 얻어진 결과를 요약하면 다음과 같다. 1) Inulin을 분해하는 효소를 생산하는 Aspergillus속 1주를 선별하였다. 2) 선별된 묘주효소의 E.P차에 의한 침전은 pH8~5에서 효소단백질이 다량 회수되었다. 3) 선별된 묘주가 분비하는 Inulase활성최적 pH는 3.0부근이 었다. 4) 선별된 묘주의 Inulin 분해효소의 활성최적 온도는 $55^{\circ}C$였다. 5) 본 Sample효소의 열에 대한 안정성은 $50^{\circ}C$에서 10분간 열처리 하여도 안정하였다. 6) 본 Sample효소의 안정 pH는 2.5~4.5정도였다. 7) 본 Sample효소의 금속 ion에 대한 영향은 $Mg^{++}$는 Activator였으며 $Sb^{+++}$, $Bi^{+++}$.는 저농도에서는 inhibitor였으나 $10^{-4}$ M~$10^{-4}$ M 농도일때는 촉진시켰으며 더욱 금속 ion농도가 높아짐에 따라 inhibition하였으며 $Ag^{++}$, $Hg^{++}$의 경우에는 저농도($10^{-8}$ M)일 때는 Activation하였고 반면, 농도가 높아짐에 따라 저해효과가 더욱 컸으며 특히 $Hg^{++}$의 경우 $10^{-2}$ M 농도에서 약 80% 저해를 나타내었다.내었다.

• 한국식품과학회지
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• 제41권2호
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• pp.167-172
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• 2009

거품성능과 겔형성능이 우수한 것으로 확인된 난백분말(pH 6.34, 72.5$^{\circ}C$; pH 6.74, 66.5$^{\circ}C$; pH 7.72, 76$^{\circ}C$)과 상업용 난백분말로 각각 엔젤 푸드 케이크를 제조하여 일반적인 특성을 조사하였다. pH 6.74, 66.5$^{\circ}C$와 pH 7.72, 76$^{\circ}C$의 조건으로 제조된 난백분말로 만든 엔젤 푸드 케이크의 거품과 반죽의 비중, 케이크의 부피 및 물성 등을 측정한 결과는 난백을 사용하여 만든 대조구와 차이가 없는 것으로 나타나고 있으나 상업용 난백분말로 제조한 케이크는 모든 면에서 크게 떨어지는 것으로 나타났다. 종합적 기호도 면에서는 처리 조건 pH 6.74, 66.5$^{\circ}C$의 난백분말로 만든 엔젤 푸드 케이크만이 대조구와 유사하게 평가되고 나머지는 다소 낮았으며, 상업용 난백분말로 만든 엔젤 푸드 케이크는 아주 낮았다. 따라서 난백의 pH를 6.74로 조정한 후 건조온도 66.5$^{\circ}C$에서 제조한 난백분말은 엔젤 푸드 케이크를 만드는데 적합한 조건으로 나타났다.

• Journal of Korean Medical Science
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• 제33권52호
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• pp.335.1-335.17
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• 2018

Background: Spontaneous bacterial peritonitis (SBP) is a serious infectious complication in patients with liver cirrhosis. However, information about prognosis of SBP in hepatocellular carcinoma (HCC) patients is limited. We investigated the clinical course of SBP in HCC patients. Methods: This study enrolled patients diagnosed with SBP between 2005 and 2017. Medical records of patients were reviewed and clinical course was compared between the non-HCC and HCC groups. Results: In total, 123 SBP cases including 49 HCC cases were enrolled. Men were predominant (48/74, 64.9% vs. 34/49, 69.4%; P = 0.697); median age was 58 years in both non-HCC and HCC groups (P = 0.887). The most common etiology was alcohol (32/74, 43.2%) in non-HCC group and hepatitis B (30/49, 61.2%) in HCC group (P = 0.009). Antibiotic resistance rate was higher in non-HCC than in HCC group (29.7% vs. 12.2%; P = 0.028); in-hospital mortality did not differ between the groups (25/74, 33.8% vs. 13/49, 26.5%; P = 0.431). Development rate of hepatorenal syndrome did not differ between non-HCC and HCC group (14/74, 18.9% vs. 10/49, 20.4%; P = 1.000), but hepatic encephalopathy was less common in HCC group (26/74, 35.2% vs. 9/49, 18.3%; P = 0.008). The most important predictor of in-hospital mortality in patients with HCC was white blood cell count above $11,570cells/mm^3$ (odds ratio, 6.629; 95% confidence interval, 1.652-26.590; P = 0.008). Conclusion: Prognosis of SBP in HCC patients is relatively less severe. This result may be related with reduced antibiotics resistance and lower development rates of other complications, such as hepatic encephalopathy. Degree of systemic inflammation may be the most important factor for in-hospital mortality.

• BMB Reports
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• 제37권6호
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• pp.700-708
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• 2004

The genes located within the p74 gene region of the Choristoneura fumiferana granulovirus (ChfuGV) were identified by sequencing an 8.9 kb BamHI restriction fragment on the ChfuGV genome. The global guanine-cytosine (GC) content of this region of the genome was 33.02%. This paper presents the ORFs within the p74 gene region along with their transcriptional orientations. This region contains a total of 15 open reading frames (ORFs). Among those, 8 ORFs were found to be homologues to the baculoviral ORFs: Cf-i-p , Cf-vi, Cf-vii, Cf-viii (ubiquitin), Cf-xi (pp31), Cf-xii (lef-11), Cf-xiii (sod) and Cf-xv-p (p74). To date, no specific function has been assigned to the ORFs: Cf-i, Cf-ii, Cf-iii, Cf-iv, Cf-v, Cf-vi, Cf-vii, Cf-ix and Cf-x. The most noticeable ORFs located in this region of the ChfuGV genome were ubiquitin, lef-11, sod, fibrillin and p74. The phylogenetic trees (constructed using conceptual products of major conserved ORFs) and gene arrangement in this region were used to further examine the classification of the members of the granulovirus genus. Comparative studies demonstrated that ChfuGV along with the Cydia pomonella granulovirus (CpGV), Phthorimaea operculella granulovirus (PhopGV), Adoxophyes orana granulovirus (AoGV) and Cryptophlebia leucotreta granulovirus (ClGV) share a high degree of amino acids sequence and gene arrangement preservation within the studied region. These results support a previous report, which classified a granuloviruses into 2 distinct groups: Group I: ChfuGV, CpGV, PhopGV and AoGV and Group II: Xestia c-nigrum granulovirus (XcGV) and Plutella xylostella granulovirus (PxGV). The phylogenetic and gene arrangement studies also placed ClGV as a novel member of the Group I granuloviruses.

• Journal of Dairy Science and Biotechnology
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• 제31권2호
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• pp.143-152
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• 2013

본 연구는 김치로부터 GABA 생성능이 우수한 젖산균을 분리 및 동정하고, 이 균주의 생리적 특성을 규명하여 상업적으로의 이용가능성을 검토하고자 실시하였다. 이를 위해 MSG 2%가 첨가된 MRS broth에 $37^{\circ}C$에서 18시간 배양한 후 GABA 함량이 $50{\mu}g/mL$ 이상 생산하는 75개의 균주를 1차로 선별하였으며, $90{\mu}g/mL$ 이상 생산하는 5 균주를 2차로 선별하였고, 선발된 5 균주를 MSG 1%, 2% 및 3% 함유된 MRS 배지에 각각 배양한 결과, K74 균주가 최종 선발되었다. K74 균주는 MSG 1%가 첨가된 MRS broth에서 GABA 함량이 $134.52{\mu}g/mL$이었고, MSG 2%와 3%가 첨가된 MRS broth에서GABA 함량이 각각 $212.27{\mu}g/mL$와 $234.63{\mu}g/mL$이었으며, 동정 결과 L. plantarum K74라고 명명되었다. L. plantarum K74의 최적 생장 온도는 $34^{\circ}C$이었으며, pH 4.4에 도달하기까지 18시간이 소요되었다. L. plantarum K74는 또한 답즙산과 산성의 pH에서 모두 우수한 생존력을 나타냈으며, 발암효소인 ${\beta}$-glucuronidase를 생성하지 않고 유당분해효소인 ${\beta}$-galactosidase를 약간 생성하는 것으로 나타났다. 항생제 내성 실험 결과 Kanamycin, Polymyxin B, Streptomycin에 내성을 Novobiocin, Bacitracin, Penicillin-G, Methicillin에감수성을나타냈으며, Escherichia coli와Salmonella typhimurium에 각각 54.9%와 46.3%의 억제 효과를 지니고 있고, Staphyloccous aureus에 대해서는 거의 억제 효과가 없는 것으로 나타났다.

• 한국축산시설환경학회지
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• 제7권3호
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• pp.165-168
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• 2001

육성비육돈의 분뇨배설량 및 분뇨특성을 조사한 결과 시험기간중 사료섭취량은 3.07 kg/일.두, 음수량은 3.84kg/일.두였으며, 평균 분뇨 배설량은 각각 1.80kg/일.두, 2.76kg/일 .두로 총 4.57kg의 분뇨를 배설하는 것으로 조사되었다. 이화학적 특성에서 돈분의 수분 함량은 74.1%, pH 6.5, 질소 0.89%, $P_2O_5$ 0.46%였으며, BOD 및 SS는 각각 74,224m/l, 261,089mg/l였다. 돈뇨의 경우에는 수분 98.2%, pH 7.5, 질소 0.83%, P$_2$O$_{5}$ 0.05% 였으며, BOD 및 SS는 각각 6,054mg/l, 453mg/l였다.

• 한국세라믹학회:학술대회논문집
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• 한국세라믹학회 2000년도 추계총회,초청강연,특별강연,연구발표회
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• pp.74.2-74
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• 2000

• 천문학회보
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• 제32권1호
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• pp.74.2-74.2
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• 2007