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Changes of Physicochemical and Sensory Characteristics in Vacuum-Packaged Beef Loin during Cold Storage Time (진공포장한 소등심육의 냉장저장중 이화학적, 관능적 특성의 변화)

  • 정인철;문윤희;홍대진;김미숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.2
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    • pp.214-219
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    • 1998
  • This study was carried out to investigate the changes of physicochemical and sensory characteristics according to cold storage period of the vacuum-time beef loin. The pH was decreased for 10 days, and then was increased gradually during storage time. The lactic acid content during the initial storage was 483mg/100g, after storage for 10 days it was increased significantly (p<0.05) to 625mg/100g, and then was decreased with storage time. Regarding of color difference, the L values were 41.0~42.5, but after storage for 40 days they were increased significantly(p<0.05) to 46.2, the a and b values wee 17.3~14.3 and 7.2~5.8, respectively, they were no significant differences depending n storage time. The shear force values showed 584 and 560g for 0 and 10 days, respectively but after storage for 20 days it was decreased significntly(p<0.05) to 299g. The myofibrillar protein extractability was no significant difference for 20 days, howener, it was increased remarkably at 30 days(p<0.05). The myofibrillar fragmentation index was increased significantly (p<0.05) on 20 and 40 days, and the Mg-ATPase activity of myofibrils was increasd to 30 days. The free amino acid was increased during storage periods. The composition of amino acid was composed of glutamic acid, alanine, valine and lysine, which were predominant amino acids as 45%. The total free amino acid was increased 182.18mg/100g to 40 days. The raw meat aroma showed no significant changes during storage time, but the tenderness was increased until 30 days(p<0.05). The color was superior from 20 to 30 days. The taste of cooked meat indicated significant changer to 30 days, but was significantly inferior(p<0.05) at 40 days, the aroma was superior until 30 days(p<0.05). The palatability was superior between 20 and 30 days.

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Effects of Drainage Depths on Agronomic Characteristics, Yield and Feed Value of Silage Corn Hybrid in Paddy Field of Lowland (저지대 논 토양에서 배수로 깊이가 사일리지용 옥수수의 생육특성 및 사료가치에 미치는 영향)

  • Lee, Sang Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.35 no.2
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    • pp.137-144
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    • 2015
  • This study was carried out to investigate the growth characteristics, yield, and chemical compositions of corn hybrids for silage according to the drainage depths in a lowland paddy field. The experimental design was arranged in a randomized block design with four treatments and three replications. The drainage depths of the four treatments were 0 cm, 20 cm, 30 cm and 50 cm. Tip-filling degree, ear length, and ear circle were higher in the 30 cm and 50 cm treatments compared with the other treatments (p < 0.05); but the plant length, leaf length, leaf width, ear height, stem diameter, and stem hardness were not significantly different (p < 0.05). The brix was higher in the order of 20 cm > 0 cm > 50 cm > 30 cm treatment. The fresh yield, dry matter yield, and TDN yield increased as the drainage depth increased (p < 0.05). The crude-protein and crude-ash content were highest in the 50 cm treatment (p < 0.05), but the crude-fat, NDF, ADF, and crude-fiber levels were not significantly different (p < 0.05). The total mineral content was higher in the order of 0 cm > 30 cm > 20 cm > 50 cm treatment (p < 0.05). Fructose, glucose, and sucrose were highest in the 20 cm treatment. The total amino-acid (EAA + NEAA) content was the highest in the 50 cm treatment. Based on these results, greater drainage depths increased the yield and feed value of silage corn.

Histopathological Study of Acute Toxicity of Ammonia on Common Carp Cyprinus carpio (잉어, Cyprinus carpio에 미치는 암모니아 급성독성의 조직병리학적 연구)

  • YANG Han Choon;CHUN Seh-Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.3
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    • pp.249-256
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    • 1986
  • The carp (Cyprinus carpio) used in the experiment were hatched in the spring this year and reared to 5.96g($4.84{\sim}6.55g$) in mean weight in a nursery pond at Daeyon fish farm, Pusan, Korea. The sample fish were exposed to different conditions of total ammonia (TA-N) concentrations 10, 20 and 30ppm and pH 6.5, 7.0, 7.5 and 8.0 at water temperatures 20, 25 and $30^{\circ}C$ for 24, 48 and 72 hours. After the procedure, the gill, liver and kidney of the fish were examined histopathologically. In this experiment, with the rise of water temperature, increase of pH and ammonia concentration, and the extension of exposure time the three organs showed the tendency of apparent abnormal changes such as hypertrophy and necrosis in their tissues. At $20^{\circ}C$ of water temperature gill tissue did not show any abnormality regardless of the change of pH at 10 ppm of ammonia concentration for 24 hours of exposure, but beyond the conditions given above, there occurred hypertrophy and the epithelium of gill lamellae was detached. The detach of gill lamellae epithelium initiated from the proximal part of the gill lamellae then gradually spread toward the uppermost tip. The heavier vacuolation of the liver was observed with the rise of water temperature and pH, and such morbid state in the liver was considered to be the result of edema in the liver tissue. The kidney showed no damage to the renal tubule epithelium at pH 6.5, but it was damaged at pH 8.0 when exposed to 30 ppm ammonia at $20^{\circ}C$ for 24 hours.

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Immunohistochemical evaluation of p63 and cyclin D1 in oral squamous cell carcinoma and leukoplakia

  • Patel, Sunit B.;Manjunatha, Bhari S.;Shah, Vandana;Soni, Nishit;Sutariya, Rakesh
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.43 no.5
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    • pp.324-330
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    • 2017
  • Objectives: There are only a limited number of studies on cyclin D1 and p63 expression in oral squamous cell carcinoma (OSCC) and leukoplakia. This study compared cyclin D1 and p63 expression in leukoplakia and OSCC to investigate the possible correlation of both markers with grade of dysplasia and histological grade of OSCC. Materials and Methods: The study included a total of 60 cases, of which 30 were diagnosed with OSCC and 30 with leukoplakia, that were evaluated immunohistochemically for p63 and cyclin D1 expression. Protein expression was correlated based on grades of dysplasia and OSCC. Results: Out of 30 cases of OSCC, 23 cases (76.7%) were cyclin D1 positive and 30 cases (100%) were p63 positive. Out of 30 cases of leukoplakia, 21 cases (70.0%) were cyclin D1 positive and 30 (100%) were p63 positive (P<0.05). Conclusion: The overall expression of cyclin D1 and p63 correlated with tumor differentiation, and increases were correlated with poor histological grades, from well-differentiated to poorly-differentiated SCC. Increased cyclin D1 and p63 expression was associated with the severity of leukoplakia. Based on these results cyclin D1 and p63 products can be a useful tool for improved leukoplakia prognosis.

Carbon Storage of Pure and Mixed Pine-Deciduous Oak Forests in Gwangneung, Central Korea

  • Lee, Sue-Kyoung;Son, Yo-Whan;Noh, Nam-Jin;Yoon, Tae-Kyung;Lee, Ah-Reum;Seo, Kyung-Won;Hwang, Jae-Hong;Bae, Sang-Won
    • Journal of Ecology and Environment
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    • v.32 no.4
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    • pp.237-247
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    • 2009
  • This study was conducted to determine the carbon (C) contents in different mixed stands of P. dens if/ora and deciduous oak species in Gwangneung, central Korea. Five mixed stands with different ratios of P. densiflora and deciduous oak species were chosen based on the basal area of all trees ${\geq}\;5cm$ DBH: pure P. densiflora (P100D0), 70% P. densiflora + 30% deciduous oak species (P70D30), 44% P. densiflora + 56% deciduous oak species (P50D50), 37% P. densiflora + 63% deciduous oak species (P40D60), and 10% P. densiflora + 90% deciduous oak species (P10D90). Total C contents in the overstory (aboveground and belowground) vegetation were higher in the mixed stands (P70D30, P50D50, P40D60) than in the pure stands (P100D0, P10D90). Moreover, except for P40D60, C contents of forest floor (litter and coarse woody debris) were larger in the mixed stands (P70D30, P50D50) than in the pure stands. However, total soil C contents up to 30cm depth were highest in the pure deciduous oak stand than in the pure P. densiflora stand and mixed stands. Total ecosystem C contents (Mg/ha) were 163.3 for P100D0, 152.3 for P70D30, 188.8 for P50D50, 160.2 for P40D60, and 150.4 for P10D90, respectively. These differences in total ecosystem C contents among the different mixed stands for P. densiflora and deciduous oak species within the study stands were attributed by the differences in vegetation development and forest management practices. Among the five study stands, the total ecosystem C contents were maximized in the 1:1 mixed ratio of P. densiflora and deciduous oak species (P50D50).

Impact of Environmental Factors on Phosphorus Removal of Bacillus licheniformis Isolated from Domestic Sewage (생활하수에서 분리된 Bacillus licheniformis의 인 제거에 대한 환경적인 인자의 영향)

  • Han, Seok-Soon;Park, Sang-Wook;Kim, Deok-Won;Park, Ji-Su;Oh, Eun-Ji;Yoo, Jin;Kim, Deok-Hyeon;Chung, Keun-Yook
    • Journal of Environmental Science International
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    • v.30 no.2
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    • pp.161-172
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    • 2021
  • This study was initiated to isolate the microorganisms removing phosphorus (P) from domestic sewage and to investigate the effects of environmental factors on the growth and P removal of the isolated bacteria. Microorganisms isolated from the sewage were identified as Chryseobacterium sp., Stenotrophomonas maltophilia, and Bacillus licheniformis. Among them, Bacillus licheniformis was selected as the P removal microorganism. The environmental factors considered in this study included initial phosphorus concentration, temperature, pH, and carbon source. At initial P concentrations of 10, 20, and 30 mg/L, the P removal efficiencies were 100.0%, 84.0%, and 16.5%, respectively. At 20℃, 30℃, and 40℃, the P removal efficiencies were 0%, 75.8%, and 60.6%, respectively. The removal efficiencies of phosphorus according to pH were 1.6%, 91.7%, and 51.1% at pH 5, pH 7, and pH 9, respectively. Using glucose, acetate, and glucose + acetate as carbon sources yielded P removal efficiencies of 80.9%, 33.6%, and 54.1%, respectively. Therefore, the results from the study demonstrated that the P removal efficiencies of Bacillus licheniformis were the highest when the initial P concentration, temperature, pH, and carbon source were 10 mg/L, 30℃, 7, and glucose, respectively.

Effects of Copper and Zinc Supplementation on Growth Performance, Nutrient Digestibility, Meat and Carcass Characteristics in Finishing Pigs (구리 및 아연의 수준별 급여가 비육돈의 생산성, 영양소 소화율, 육질 및 도체 특성에 미치는 영향)

  • Kim, Y.H.;Kim, H.J.;Park, J.C.;Jung, H.J.;Cho, J.H.;Chen, Y.J.;Yoo, J.S.;Kim, I.C.;Lee, S.J.;Kim, I.H.
    • Food Science of Animal Resources
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    • v.27 no.3
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    • pp.284-289
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    • 2007
  • This study was conducted to investigate the effects of copper and zinc supplementation on growth performance, nutrient digestibility, and meat and carcass characteristics in finishing pigs. A total of 72 $(Landrace{\times}Yorkshire{\times}Duroc)$ pigs (58.47 kg initial BW) were assigned to 6 treatments in a $2{\times}3$ factorial design $(Zn\;levels{\times}Cu\;levels)$. The sources of zinc and copper were Zn-methionine chelate and Cu-methionine chelate, respectively. Zinc levels used were 80 and 120 ppm and copper levels used were 10, 30 and 60 ppm. Throughout the entire experimental period, the average daily gain (ADG) and average daily feed intake (ADFI) were not significantly affected by Cu or Zn levels, or their relative levels. The G:F ratio was significantly affected by the relative levels of Cu and Zn (p<0.05), specifically at 30 ppm Cu and 120 ppm Zn. Dry matter digestibility was significantly affected by the levels of Cu (p<0.02), Zn (p<0.01) and the relative levels of each (p<0.04), in particular at 30 ppm Cu and 120 ppm Zn. Nitrogen digestibility was significantly affected by Zn levels (p<0.01) and the combination of 30 ppm Cu and 120 ppm Zn (p<0.03). The $L^*-value$, shear force, cooking loss and pH were not significantly affected by Cu levels, Zn levels or their combination. The $a^*- (p<0.04)\;and\;b^*- values (p<0.01)$ were significantly affected by Zn levels at 80 ppm. The Water hoding capacity was significantly affected by Cu and Zn in combination (p<0.01) at 10 ppm Cu and 120ppm Zn. The carcass weight, backfat thickness and carcass grade were not significantly affected by Cu levels, Zn levels or their relative levels. The carcass percentage was significantly affected by the combination (p<0.04) of 30ppm Cu and 120ppm Zn. In conclusion, dietary supplementation of Cu and Zn at 30 and 120 ppm, respectively, is effective for feed efficiency, nutrient digestibility and carcass percentage, while at the levels of Cu at 10 ppm and Zn at 120 ppm have effects on WHC.

Effects of Dietary Supplementation of Lysine Cell Mass as a Fish Meal Replacer in Juvenile Nile Tilapia (치어기 틸라피아에 있어 사료내 어분 대체원으로서 라이신 부산물의 첨가효과)

  • 김강웅;이진영;배승철;이희석
    • Journal of Aquaculture
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    • v.14 no.3
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    • pp.197-203
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    • 2001
  • To replace fish meal (FM) in the diet of Nile tilapia, Oreochromis nloticus, different levels of Iysine cell mass (LCM) were added to diet on protein equivalent base. fish averaging 3.0 g fed one of nine diets containing isonitrogenous and isocaloric basis of 35% crude protein and 15.3kJ available energy/g diet: $LCM_0, 100% FM: LCM_{10}, 90% FM+10% LCM, LCM_{20}, 80% FM+20% LCM; LCM_{30}, 70% FM+30% LCM; LCM_{40}, 60% FM+40% LCM; LCM_{30}l, 70% FM+30% LCM+lysine; LCM_{30}ln, 70% FM +30% LCM + lysine +NaOH; LCM_{40}lan, 60% FM+40% LCM+lysine+arginine; LCM_{40}/lan, 60% FM+40% LCM+lysine+arginine+NaOH.$. After 8 weeks of feeding trial, there were no significant differences in weight gain (WG) and specific growth rate (SGR) among fish fed LCM$_{0}$ (control diet), LCM_{10}, LCM_{30}, LCM_{40}, LCM_{30}l, LCM_{30}ln, LCA_{40}la and LCM_{40}lan diets (P>0.05)$, while fishes fed $LCM_{20} diet were significantly higher than those fed LCM_{0}, LCM_{30}, LCM_{40}, LCM_{40}la and LCM_{40$}lan diets (P<0.05). There were no significant differences in feed efficiency (FE) and protein efficiency ratio (PER) among fish fed control diet, $LCM_{10}, LCM_{20}, LCM_{30}ln, LCM_{ 40}la and LCM_{40}$lan diets (P>0.05), while fishes fed control diet were significantly higher than those fed $LCM_{30}, LCM_{40} and LCM_{40}l diets (P<0.05)$. Positive effects were not shown in WG and SGR with supplementation of amino acids (lysine & arginine) and neutralizatio, while FE and PER from fish fed $LCM_{40}la and LCM_{40}lan diets were significantly higher than those fed LCM_{30}, LCM_{40} and LCM_{30}$l diets (P<0.05). Hence, LCM can replace FM up to 40%, and dietary suppl-ementacon and neutralization of amino acids showed positive effects, when FE and PER were considered in juvenile tilapia diet.

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A GDD Model for Super Sweet Corn Grown under Black P. E. Film Mulch (흑색 P. E. Film 피복에서 초당옥수수의 생육기간을 표시하는 GDD모델 개발)

  • Lee, Suk-Soon;Yang, Seung-Kyu;Hong, Seung-Beom
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.1
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    • pp.42-49
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    • 2008
  • GDD models of corn were developed in bare soil, while sweet and super sweet corns are grown under black polyethylene (P. E.) film mulch in Korea. To develop a suitable GDD model under black P. E. film mulch, a super sweet com hybrid "Cambella-90" was planted from 1 April to 30 June in 2003 at the 10-day intervals under black P. E. film mulch and in bare soil. In bare soil the best GDD model was $GDD\;=\;{\sum}[H"+L')/2\;-\;10^{\circ}C]$, where H" was daily maximum temperature but is was substituted for $30^{\circ}C$ - (daily maximum temperature - $30^{\circ}C$) when higher than $30^{\circ}C$ and L' was daily minimum temperature, but it was substituted for $10^{\circ}C$ when lower than $10^{\circ}C$. The same GDD model could be adapted for com grown under black P. E. film mulch, but base temperature was substituted for $9^{\circ}C$. To determine planting date for the scheduled harvests, accumulated GDDs were calculated using 30-year average temperature data during the growing season. Under black P. E. film mulch planting dates were determined by subtracting GDD of the hybrid, $970^{\circ}C$, from accumulated GDD of scheduled harvest dates.

A study on the characteristics and noodle structure made from pea starch-wheat composite flour using a scanning electron microscopy (Scanning Electron Microscopy을 이용한 완두 전분 복합면의 반죽구조 및 특성연구)

  • 김은주;윤재영;김희섭
    • Korean journal of food and cookery science
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    • v.15 no.5
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    • pp.500-506
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    • 1999
  • Scanning electron microscopy was used to study changes in granule shape, dough and cooked noodle structure of pea starch-wheat composite flour with 20% and 30% pea starch substitution. The granule shape of pea starch with low swelling power and solubility was oval, irregular and smooth, which had more a deep groove than corn starch and wheat flour. During gelatinization, pea starch after swelling was partially collapsed but it still held its main shape. The dough microstructure of 20% pea starch substitution showed compact structure distributed with more small starch granules than wheat dough and was held in discontinuous network. When cooked, more open filamentous network where starch gelatinization was complete were noticed. Swollen but partially collapsed large starch granules maintaining their shape were appeared in noodle structure after 30 min soaking in soup. In farinograph studies, 20% pea starch substitution to wheat flour showed that MTI value was as same as wheat flour even though stability was slightly decreased so that it was considered that it has proper property of noodle making.

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