Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Effects of Dietary Supplementation of Lysine Cell Mass as a Fish Meal Replacer in Juvenile Nile Tilapia

치어기 틸라피아에 있어 사료내 어분 대체원으로서 라이신 부산물의 첨가효과

  • 김강웅 (부경대학교 양식학과 사료영양연구소) ;
  • 이진영 (부경대학교 양식학과 사료영양연구소) ;
  • 배승철 (부경대학교 양식학과 사료영양연구소) ;
  • 이희석 (한국바스프 주식회사)
  • Published : 2001.09.01
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Abstract

To replace fish meal (FM) in the diet of Nile tilapia, Oreochromis nloticus, different levels of Iysine cell mass (LCM) were added to diet on protein equivalent base. fish averaging 3.0 g fed one of nine diets containing isonitrogenous and isocaloric basis of 35% crude protein and 15.3kJ available energy/g diet: $LCM_0, 100% FM: LCM_{10}, 90% FM+10% LCM, LCM_{20}, 80% FM+20% LCM; LCM_{30}, 70% FM+30% LCM; LCM_{40}, 60% FM+40% LCM; LCM_{30}l, 70% FM+30% LCM+lysine; LCM_{30}ln, 70% FM +30% LCM + lysine +NaOH; LCM_{40}lan, 60% FM+40% LCM+lysine+arginine; LCM_{40}/lan, 60% FM+40% LCM+lysine+arginine+NaOH.$. After 8 weeks of feeding trial, there were no significant differences in weight gain (WG) and specific growth rate (SGR) among fish fed LCM$_{0}$ (control diet), LCM_{10}, LCM_{30}, LCM_{40}, LCM_{30}l, LCM_{30}ln, LCA_{40}la and LCM_{40}lan diets (P>0.05)$, while fishes fed $LCM_{20} diet were significantly higher than those fed LCM_{0}, LCM_{30}, LCM_{40}, LCM_{40}la and LCM_{40$}lan diets (P<0.05). There were no significant differences in feed efficiency (FE) and protein efficiency ratio (PER) among fish fed control diet, $LCM_{10}, LCM_{20}, LCM_{30}ln, LCM_{ 40}la and LCM_{40}$lan diets (P>0.05), while fishes fed control diet were significantly higher than those fed $LCM_{30}, LCM_{40} and LCM_{40}l diets (P<0.05)$. Positive effects were not shown in WG and SGR with supplementation of amino acids (lysine & arginine) and neutralizatio, while FE and PER from fish fed $LCM_{40}la and LCM_{40}lan diets were significantly higher than those fed LCM_{30}, LCM_{40} and LCM_{30}$l diets (P<0.05). Hence, LCM can replace FM up to 40%, and dietary suppl-ementacon and neutralization of amino acids showed positive effects, when FE and PER were considered in juvenile tilapia diet.

이 연구는 치어기 틸라피아에 있어서 어분(fish meal, FM) 대체 단백질원으로서 라이신 부산물(lysine cell mass, LCM)이 사료내 이용 가능성과 대체 수준을 결정하기 위해 수행하였다 실험사료는 조단백질 35%, 가용성 에너지 15.3 kJ/kg으로 설계되었고, 주 단백질원으로 어분, 대두박, 콘글루텐밀 및 라이신 부산물을 사용하였다. 주 동물성 단백질원인 어분 단백질을 라이신 부산물에 의한 대체 수준에 따라 사료 조성을 요약하면 다음과 같다: $LCM_0, 100% FM: LCM_{10}, 90% FM+10% LCM, LCM_{20}, 80% FM+20% LCM; LCM_{30}, 70% FM+30% LCM; LCM_{40}, 60% FM+40% LCM; LCM_{30}l, 70% FM+30% LCM+lysine; LCM_{30}ln, 70% FM +30% LCM + lysine +NaOH; LCM_{40}lan, 60% FM+40% LCM+lysine+arginine; LCM_{40}/lan, 60% FM+40% LCM+lysine+arginine+NaOH.$ 8주간의 실험 결과, 증체율과 일간성장률에 있어서는 사료구 $LCM_0$와 비교하여 모든 사료구에서는 유의적인 차이가 없었으며, 아울러 아미노산 첨가 및 중화효과는 모든 사료구에서 나타나지 않았다(p>0.05). 사료효율과 단백질 전환효율에 있어서 사료구 LCMo는 사료구 $LCM_{10}. LCM_{20}. LCM_{30}lan, LCM_{40}lan 및 LCM_{40}lan$과 비교하여 유의적인 차이를 보이지 않은 반면에(P>0.05), 사료구 $LCM_{30}, LCM_{40} 및 LCM_{30}l$보다 유의적으로 높게 나타났다(P<0.05). 아미노산 첨가와 중화효과에 있어서 사료구 $LCM_P{30}la, LCM_{30}l 및 LCM_{30}ln$은 유의적인 차이가 나타나지 않은 반면에 사료구 $LCM_{40}la 및 CM_{40}lan은 LCM_{40}$과 비교하여 유의적으로 높게 나타났다(P<0.05). 따라서, 치어기 틸라피아에 있어서 성장을 기준으로 라이신 부산물은 어분 단백질을 40%까지 대체가 가능하였으며, 아울러 높은 라이신 부산물의 대체 수준에 있어서 사료효율과 단백질 전환효율을 고려한다면 아미노산 첨가(라이신과 아르지닌)와 중화 효과에 좋은 결과가 있을 것으로 사료된다.

Keywords

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