• Korean Journal of Materials Research
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• v.20 no.3
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• pp.155-160
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• 2010

In this study, we investigated primary biocompatibility and osteogenic gene expression of porous granular BCP bone substitutes with or without strontium (Sr) doping. In vitro biocompatibility was investigated on fibroblasts like L929 cells and osteoblasts like MG-63 cells using a cell viability assay (MTT) and one cell morphological observation by SEM, respectively. MTT results showed a cell viability percent of L929 fibroblasts, which was higher in Sr-BCP granules (98-101%) than in the non-doped granules (92-96%, p < 0.05). Osteoblasts like MG-63 cells were also found to proliferate better on Sr-doped BCP granules (01-111%) than on the non-doped ones (92-99%, p < 0.05) using an MTT assay. As compared with pure BCP granules, SEM images of MG-63 cells grown on sample surfaces confirmed that cellular spreading, adhesion and proliferation were facilitated by Sr doping on BCP. Active filopodial growth of MG-63 cells was also observed on Sr-doped BCP granules. The cells on Sr-doped BCP granules were well attached and spread out. Gene expression of osteonectin, osteopontin and osteoprotegrin were also evaluated using reverse transcriptase polymerase chain reaction (RT-PCR), which showed that the mRNA phenotypes of these genes were well maintained and expressed in Sr-doped BCP granules. These results suggest that Sr doping in a porous BCP granule can potentially enhance the biocompatibility and bone ingrowth capability of BCP biomaterials.

• Journal of the Korean Magnetic Resonance Society
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• v.23 no.1
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• pp.20-25
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• 2019

The transcription factor CP2c has been recently validated as an oncogenic protein that can serve as a promising target for anticancer therapy. We have recently documented that a recombinant protein corresponding to the putative DNA-binding region (residues 63-244) of CP2c adopted two different conformers, one of which is dominated by zinc binding. However, in the present study, a longer construct encompassing residues 63-302 appeared to form a single structural domain. This domain could be considered to adopt a functionally relevant fold, as the known specific binding of a dodecapeptide to this protein was evident. Hence, the residues 63-302 region rather than 63-244 can be regarded as a natively folded structural domain of CP2c. In addition, it was confirmed that zinc ions can bind to this putative DNA-binding domain of CP2c, which resulted in reduced stability of the protein. In this context, it is suggested that the mode of action of CP2c would resemble that of tumor suppressor p53.

• Journal of Animal Environmental Science
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• v.6 no.3
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• pp.185-190
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• 2000

TAO system and solid-liquid separation (28mesh filter and 200mesh filter) were applied in processing piggery slurry to see the reduction of N and P and to draw the efficiency in reducing manure application area. The results are as follows; 1. The amount of N and P in slurry of $4.6m^3/day$ was 22.5kg/day ($4,893mg/{\ell}$) and 7.32kg/day ($1,592mg/{\ell}$). 2. Reduction rate of N and P by TAO reactor was 9.9 kg/day (46.0%) and 3.47kg/day(34.0%). 3. Reduction rate of N and P by Solid-liquid separation was 10.5kg/day (46.6%) and 5.12kg/day (69.8%). 4. One the basis of the amount of nitrogen composting, the square size of liquid manure sprinkled area was reduced from 74.6ha/y to 39.0ha/y in rice paddy, and from 63.2ha/y to 33.0ha/y by the treatment.

• Journal of Digital Convergence
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• v.13 no.6
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• pp.185-195
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• 2015

This study aims to study the change in the level of awareness of medical personnels regarding the patient safety culture(PSC) before and after the Leadership WalkRounds(LWR). The LWR in this study was based on the IHI and the models of the Patient Safety Rounds(PSRs) at University of Michigan, emphasizing the 5 steps of Preparation--Scheduling--Conducting--Reporting--Resolving. After the LWR the scores for the level of awareness showed a statistically significant increase from 2.63 to 3.36(p<.001). Among the participants, those who are pharmaceutists, women, 30.0~39.9 years old, or had work experience of a year or less showed particularly notable increase in awareness. The effect was significant across all categories of PSC, especially in Safety Accident Reporting(p<.001), then in Communication(p<.001). Therefore the LWR proved an convergent concept for applying new leadership skill and the concept of patient safety management as an method to elevate the frontline staffs' awareness of PSC.

• Journal of Mushroom
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• v.13 no.1
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• pp.63-67
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• 2015

White rot fungi produce lignin-degrading enzymes such as laccase, manganese peroxidase and lignin peroxidase. These extracellular oxidases efficiently degrade recalcitrant synthetic dyestuffs with diverse chemical structures. Here, we examined the activities of lignin-degrading enzymes in Trametes versicolor using triphenyl methane dyes, crystal violet (CV) and malachite green (MG). Both dyes were decolorized by T. versicolor in solid and liquid culture conditions. T. versicolor decolorized MG more quickly than CV in both conditions. Among three ligninolytic enzymes, laccase was most abundantly found in the decolorization processes of CV and MG. However, higher activity of laccase was needed to degrade CV than MG. The much less activity of MnP was also detected. But the increase of MnP activity was well corresponded to the decolorization efficiency of CV, suggesting the involvement of MnP in CV degrading process. However, its role in the degradation process of MG is supposed to be subsidiary to laccase.

• YAKHAK HOEJI
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• v.53 no.5
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• pp.259-264
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• 2009

The present study was to investigate the effect of naringin, a flavonoid, on the pharmacokinetics of losartan in rats. Pharmacokinetic parameters of losartan in rats were determined after an oral administration of losartan (9 mg/kg) in the presence or absence of naringin (0.5, 2.5 and 10 mg/kg). The pharmacokinetic parameters of losartan were significantly altered by the presence of naringin compared with the control group (given losartan alone). Presence of naringin significantly (p<0.05, 2.5 mg/kg; p<0.01, 10 mg/kg) increased the area under the plasma concentration?time curve (AUC) of losartan by 43.7~63.0% and peak plasma concentration ($C_{max}$) of losartan by 31.7~45.5%. Consequently, the absolute bioavailability (AB) of losartan in the presence of naringin was 43.8~62.9%, which was enhanced significantly (p<0.05, p<0.01) compared to that in the oral control group (22.4%). The relative bioavailability (R.B.) of losartan increased by 1.44- to 1.63-fold in the presence of naringin. However, there was no significant change in the peak plasma concentration ($T_{max}$) and terminal half-life ($t_{1/2}$) of losartan in the presence of naringin. In conclusion, the presence of naringin significantly enhanced the oral bioavailability of losartan, implying that presence of naringin might be mainly effective to inhibit the cytochrome P450 (CYP)3A-mediated metabolism, resulting in reducing gastrointestinal and hepatic first-pass metabilism and Pglycoprotein (P-gp)-mediated efflux of losartan in small intestine. Concurrent use of naringin or naringin-containing dietary supplement with losartan should require close monitoring for potential drug interactions.

• Archives of Pharmacal Research
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• v.26 no.11
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• pp.937-942
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• 2003

Nitric oxide(NO) induces apoptosis in human osteoblasts. Treatment with exogenous NO donors, SNAP (S-Nitroso-N-acelylpenicillamine) and SNP (sodium nitroprusside), to MG-63 osteoblasts resulted in apoptotic morphological changes, as shown by a bright blue-fluorescent condensed nuclei and chromatin fragmentation by fluorescence microscope of Hoechst 33258-staining. The activities of caspase-9 and the subsequent caspase-3-like cysteine proteases were increased during NO-induced cell death. Pretreatment with Z-VAD-FMK (a pancaspase inhibitor) or Ac-DEVD-CHO (a specific caspase-3 inhibitor) abrogated the NO-induced cell death. The NO donor markedly activated JNK, a stress-activated protein kinase in the human osteoblasts. This study showed that the inhibition of the JNK pathway markedly reduced NO-induced cell death. But neither PD98059 (MEK inhibitor) nor SB203580 (p38 MAPK inhibitor) had any effect on NO-induced death. Taken together, these results suggest that JNK/SAPK may be related to NO-induced apoptosis in MG-63 human osteoblasts.

• Journal of muscle and joint health
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• v.20 no.3
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• pp.214-224
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• 2013

Purpose: The purpose of this study was to identify the differences in preference for terminal care between hospitalized patients and nurses. Methods: A cross-sectional descriptive design was used in 79 patients and 107 nurses. The data were collected from August to October 2011, using the Preference for Care near the End of life Scale - Korean Version (PCEOL-K) with 5-point scale of 26 items. The reliability of the tool was Cronbach's ${\alpha}=.74$. Results: The mean score (SD) of PCEOL-K's sub-dimensions in nurses' priority was: (a) pain $3.70{\pm}0.63$, (b) spirituality $3.63{\pm}0.61$, (c) family $3.40{\pm}0.70$, (d) autonomous decision making $2.30{\pm}0.66$, and (e) decision making by healthcare professionals $2.14{\pm}0.64$. In patients' priority, the $M{\pm}SD$ score of each sub-dimension was: (a) pain $3.86{\pm}0.65$, (b) family $3.83{\pm}0.57$, (c) decision making by healthcare professionals $3.37{\pm}0.85$, (d) spirituality $3.01{\pm}0.80$, and (e) autonomous decision making $2.43{\pm}0.63$. Results indicated significant differences between nurses and patients regarding decision making by healthcare professionals (t=-11.28, p<.001), family (t=-4.66, p<.001), and spirituality (t=5.71, p<.001). Conclusion: The PCEOL-K of patients was higher than nurses'. A terminal care program for hospitalized patients at the end of life should be planned according to the results of PCEOL-K in nurses and patients.

• Journal of Korea Water Resources Association
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• v.45 no.11
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• pp.1121-1130
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• 2012

Projected changes and their impacts on water quality are simulated in response to climate change stressors. CGHR (T63) simulation on the A1B scenario is converted to regional scale data using a statistical down-scaling method and applied to SWAT model to assess water quality impacts in Nakdong River basin. The results demonstrate that rainfall-runoff and pollutant loading in the future (2011~2100) will clearly increase as compared to the last 30-year average. The rate of pollutant loading increase is expected to continue its acceleration until 2040s. Runoff also shows similar patterns to the precipitation, increasing by 60%. Accordingly, the runoff increase results in escalation of pollutant loading by 35~45% for TSS and 5~20% for T-P. This phenomenon is more pronounced in the upper basin during winter and spring season.

• Journal of Preventive Medicine and Public Health
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• v.10 no.1
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• pp.44-51
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• 1977

In order to determine values for the body weight by height groups for Korean adults men who are fully grown up 4,028 (30-39 age-group ; 2,318, 40-49 age-group 1,340, and 50-59 age group : 370) apparently healthy males subjects were randomly selected for the measurement of body weight by height groups. 1) The avergae body height and weight of Korean adult men were $168.3{\pm}4.6cm$ and $63.4{\pm}7.1kg$ in 30-39 age group, $167.7{\pm}4.7cm$ and $63.4{\pm}7.4kg$ in 40-49 age group, and $167.3{\pm}5.2cm$ and $63.3{\pm}8.0kg$ in 50-59 age group. 2) A correlation coefficient of r=+0.52(P<0.001) between body height and weight was found in 30-39 age group of 2,318 subject, r=+0.48(P<0.001) in 40-49 of 1,340 and r=+0.53(P<0.001) in 50-59 of 370 with the aid of there coefficients of linear regression equation body weight and height were established for male as follow; for 30-39 age group, Y(weight in kg)=0.81X(height in cm)-73.02, 40-49 age group, Y(weight in kg)=0.74X(height in cm)-61.82, 50-59g age-group, Y(weight in kg)=0.82X(height in cm)-73.83. 3) With the aid of above listed various equation standard values for body weight by height group, with over weighing and under weighing values were established. 4) Standard bodyweight of Korean was lower than those of American, Japanese and several other formulas for ideal body weight.