• 한국수산과학회지
• /
• 제26권4호
• /
• pp.392-400
• /
• 1993

원문만에 대하여 저산소수괴의 출현 및 계절적 변동특성을 구명하고, 하계 저층용존산소를 일정수준 이상으로 유지하기 위한 적정오염부하량 산정을 위하여 1989년 7월부터 1991년 12월까지 약 2년 6개월 동안 조사한 결과를 요약하면 다음과 같다. 1. 저산소수괴의 계절적 변동은 매년 그해의 해황변동에 따라 크게 다르며, 1990년과 1991년은 하계 성층의 형성과 더불어 저층에서의 용존산소 저하현상이 나타났으며, 특히 1990년 원문만에서는 대체적으로 봄부터 용존산소의 저하가 시작되어 여름에는 강한 저산소 또는 무산소수괴가 형성되었다가 가을에 회복되는 양상을 나타내고 있다. 2. 저산소수괴의 시공간적 변동은 기온, 일조시간, 강우량, 바람 등에 의한 수직성층의 변동과 밀접한 관계를 나타내며, 그 해역에서의 적조발생은 저산소수괴 형성을 촉진시키는 것으로 생각된다. 3. 저층 용존산소 농도가 낮은 시기에는 영양염 농도가 표층에 비하여 저층에서 높은 분포였으며, 저층 산소가 정상적으로 회복된 시기에는 표${\cdot}$저층 영양염 농도가 비슷하거나 오히려 표층에서 높은 농도를 나타냈다. 또한, AOU와 인산인과의 상관성으로부터 AOU $4{\sim}5mg/l$ 이하에서의 직선과 AOU $4{\sim}5mg/l$ 이상에서의 급격한 기울기를 가진 2개의 직선으로 나타났다. 4. 1990년 원문만의 시간에 따른 용존산소 변화 곡선은 $DO(ml/l)=9.99-0.21{\times}(day)+0.0015{\times}(day)^2-0.0000023{\times}(day)^3+{\cdots}{\cdots}$와 같이 나타낼 수 있었고, 산소소비계수 0.0458/day, 산소공급계수 0.0157ml/l/day로서 임계점의 산소농도를 3.52ml/l 이상으로 유지시키기 위해 공급되어야 할 산소량은 0.141ml/l/day였다.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제3권1호
• /
• pp.15-18
• /
• 1998

The entanol productivity of superoxide dismutase (SOD)-deficient mutants of Saccharo-Myces cerevisiae was examined under the oxidative stress by Paraquat. It was observed that MnSOD-deficient mutant of S. cerevisiae had higher ethanol productivity than wild type or CuZnSOD-deficient yeast both in aerobic and in anaerobic culture condition. Pyruvated dehydrogenase activity decreased by 35% and alcohol dehydrogenase activity increased by 32% were observed in MnSOD-deficient yeast grown aerobically. When generating oxygen radicals by Paraquat, the ehanol productivity was increased by 40% in CuZnSOD-deficient or wild strain, resulting from increased activity of alcohol dehydrogenase and decreased a activity of pyruvate dehydrogenase. However, the addition of ascorbic acid with Paraquat returned the enzyme activities at the level of control. These results imply that SOD-deficiency in yeast strains may cause the metabolic flux to shift into anaerobic ethanol fermentation in order to avoid their oxidative damages by Paraquat.

• Molecules and Cells
• /
• 제43권3호
• /
• pp.264-275
• /
• 2020

Reactive oxygen species (ROS) play a significant role in intracellular signaling and regulation, particularly when they are maintained at physiologic levels. However, excess ROS can cause cell damage and induce cell death. We recently reported that eIF2α phosphorylation protects hepatocytes from oxidative stress and liver fibrosis induced by fructose metabolism. Here, we found that hepatocyte-specific eIF2α phosphorylation-deficient mice have significantly reduced expression of the epidermal growth factor receptor (EGFR) and altered EGFR-mediated signaling pathways. EGFR-mediated signaling pathways are important for cell proliferation, differentiation, and survival in many tissues and cell types. Therefore, we studied whether the reduced amount of EGFR is responsible for the eIF2α phosphorylation-deficient hepatocytes' vulnerability to oxidative stress. ROS such as hydrogen peroxide and superoxides induce both EGFR tyrosine phosphorylation and eIF2α phosphorylation. eIF2α phosphorylation-deficient primary hepatocytes, or EGFR knockdown cells, have decreased ROS scavenging ability compared to normal cells. Therefore, these cells are particularly susceptible to oxidative stress. However, overexpression of EGFR in these eIF2α phosphorylation-deficient primary hepatocytes increased ROS scavenging ability and alleviated ROS-mediated cell death. Therefore, we hypothesize that the reduced EGFR level in eIF2α phosphorylation-deficient hepatocytes is one of critical factors responsible for their susceptibility to oxidative stress.

• 한국해양학회지:바다
• /
• 제8권2호
• /
• pp.177-186
• /
• 2003

하계 진동만 저층수의 저산소화에 미치는 성층 및 바람의 효과를 고려한 수평 및연직 유동성분의 영향을 조사하여 내만역에서의 빈산소수괴 형성기구를 밝혔다. 진동만에서 수온, 염분, 용존산소 및 풍향, 풍속은 1998년 3월부터 1999년 2월 사이에 조사하였다 저층수에서 5 이하의 낮은 용존산소농도가 5월에서 9월에 걸쳐 존재하였으며, 표층수의 고온·저염화에 따라 강한 약층이 형성되어 연직기울기는 매우 크게 나타난 하계(7∼8월)에 3 이하의 극히 낮은 용존산소농도가 출현하였다. 이와 같은 저농도의 용존산소는 특히 만 안쪽 정점에서 춘계(5∼6월)에도 관찰되었다. 또한, 하계(8∼9월)에 5 이하의 낮은 용존산소농도를 가지는 저층수가 약 4∼6 m 수층까지 표층쪽으로 부상하는 특징적인 현상이 나타났다 여름철 진동만에서 바람의 효과를 고려한 잔차류의 연직성분을 살펴보면, 이 지역에서 우세한 북풍이나 서풍계열의 바람에 의해서 만 바깥쪽을 하강류역이, 만 안쪽은 상승류역이 발생한다. 또한, 하강류역인 만 바깥쪽에서 만 안쪽으로 향하는 저층류가 발달하였는데, 이것은 표층으로부터 침강된 유기물질의 만 안쪽으로의 유입을 초래할 수 있을 것으로 기대된다. 한편 말 안쪽에서 나타나는 상승류의 존재가 만 안쪽에서 형성된 저층의 빈산소수를 표층 근처까지 부상하게 하는 역할을 하는 것으로 보인다.

• 한국재료학회:학술대회논문집
• /
• 한국재료학회 2007년도 추계학술발표대회 및
• /
• pp.19.2-19.2
• /
• 2007

• 한국환경과학회지
• /
• 제8권5호
• /
• pp.575-586
• /
• 1999

From the environmental aspects, primary productivity of phytoplankton plays the most improtant role in enhancement of marine culture oyster production. This study may be divided into two branches; one is estimation of maximum oyster meat production per unit facility(Carrying Capacity) under the present enviromental conditions in Kamak Bay, the other is improvement of carrying from increase of primary productivity by changing the environmental conditions that cause not ot form an unfavorable environment such as the formation of oxygen deficient water mass using the eco-hydrodynamic model. By simulation of three-dimensional hydrdynamic model and ecosystem model, the comparison between observed and computed data showed good agreement. The results of sensitivity analysis showed that phytoplankton maximum growth rate was the most important parameter for phytoplankton and dissolved oxygen. The estimation of mean primary productivity of Wonpo, Kamak, Pyongsa, and Kunnae culture grounds in Kamak Bay during culturing period were 3.73gC/$m^2$/d, 2.12gC/$m^2$/d, 1.98gC/$m^2$/d, and 1.26gC/$m^2$/d, respectively. Under condition not ot form the oxygen deficient water mass, four times increasing of pollutants loading as much as the present loading from river increased mean primary productivity of whole culture grounds to 4.02gC/$m^2$/d. Sediment N, P fluxes that allowed for 35% increasing from the present conditions increased mean primary productivity of whole culture grounds to 3.65gC/$m^2$/d. Finally, ten times increasing of boundary loadings from the present conditions increased mean primary productivity of whole culture grounds to 3.95gC/$m^2$/d. The maximum oyster meat production per year and that of unit facility in actual oyster culture grounds under the present conditions were 6,929ton and 0.93ton, respectively. This 0.93ton/unit facility is considered to be the carrying capacity in study area, and if the primary productivity is increased by changing the environmental conditions, oyster production can be increased.

• 한국재료학회지
• /
• 제23권10호
• /
• pp.580-585
• /
• 2013

To observe the formation of defects at the interface between an oxide semiconductor and $SiO_2$, ZnO was prepared on $SiO_2$ with various oxygen gas flow rates by RF magnetron sputtering deposition. The crystallinity of ZnO depends on the characteristic of the surface of the substrate. The crystallinity of ZnO on a Si wafer increased due to the activation of ionic interactions after an annealing process, whereas that of ZnO on $SiO_2$ changed due to the various types of defects which had formed as a result of the deposition conditions and the annealing process. To observe the chemical shift to understand of defect deformations at the interface between the ZnO and $SiO_2$, the O 1s electron spectra were convoluted into three sub-peaks by a Gaussian fitting. The O 1s electron spectra consisted of three peaks as metal oxygen (at 530.5 eV), $O^{2-}$ ions in an oxygen-deficient region (at 531.66 eV) and OH bonding (at 532.5 eV). In view of the crystallinity from the peak (103) in the XRD pattern, the metal oxygen increased with a decrease in the crystallinity. However, the low FWHM (full width at half maximum) at the (103) plane caused by the high crystallinity depended on the increment of the oxygen vacancies at 531.66 eV due to the generation of $O^{2-}$ ions in the oxygen-deficient region formed by thermal activation energy.

• Journal of Microbiology and Biotechnology
• /
• 제23권4호
• /
• pp.560-564
• /
• 2013

${\small{D}}$-Ribose is a value-added five-carbon sugar used for riboflavin production. To investigate the effects of oxygen supply and mixed sugar concentration on microbial production of ${\small{D}}$-ribose, a transketolase-deficient Bacillus subtilis SPK1 was cultured batch-wise using xylose and glucose. A change of agitation speed from 300 rpm to 600 rpm at 1 vvm of air supply increased both the xylose consumption rate and ${\small{D}}$-ribose production rate. Because the sum of the specific consumption rates for xylose and glucose was similar at all agitation speeds, metabolic preferences between xylose and glucose might depend on oxygen supply. Although B. subtilis SPK1 can take up xylose and glucose by the active transport mechanism, a high initial concentration of xylose and glucose was not beneficial for high ${\small{D}}$-ribose production.

• 한국세라믹학회지
• /
• 제28권8호
• /
• pp.603-610
• /
• 1991

An oxygen deficient perovskite (CaLa)(MgMn)O5.43, with the cubic unit cell parameter of 3.826$\AA$, was prepared 115$0^{\circ}C$ for 10 hrs under the ambient oxygen gas pressure. The average oxidation state of manganese was determined to be 3.86 by the iodometric titration, so that the perovskite could be formulated as (CaLa) ({{{{ { MgMn}`_{ chi } ^{II } }}{{{{ { Mn}`_{ y} ^{III } }}{{{{ { Mn}`_{1- chi -y } ^{IV } }})O5.43 (2x+y=0.14). From X-ray photoelectron spectroscopy, the manganese ions in the lattice are mostly tetravalent, but two paramagnetic configurations were observed in the EPR spectrum: One sharp isotropic signal with hyperfines (ΔH 50 G, g=1.997$\pm$0.002 and │A│=82(4)$\times$10-4 cm-1) and a broad isotropic one (ΔH 1600 G, g=1.994$\pm$0.002), those which correspond respectively to Mn(II) and Mn(IV) ions. According to the magnetic susceptibility measurement, it follows the Curie-Weiss law from 20 K up to room temperature with $\mu$eff=5.23 $\mu$B, which is relatively larger than spin-only value({{{{ { mu }`_{eff} ^{s.o } }}=4.04 $\mu$B) due to the effect of weak ferromagnetic coupling. Such a result is in accord with a theory of semicovalence exchange.

• Biomolecules & Therapeutics
• /
• 제17권2호
• /
• pp.144-150
• /
• 2009

Reactive oxygen species play a role in signal transduction and in many human diseases. B-cell activating factor (BAFF) plays a role for mature B cell generation and maintenance and for the incidence of autoimmune diseases. We previously reported that BAFF expression was induced by ROS. In this study, we investigated whether ROS-induced BAFF expression was affected by toll-like receptor (TLR) 4 or myeloid differentiation primary response gene (MyD) 88. BAFF expression was increased by serum deprivation that is an experimental modification to produce ROS. In contrast, TLR4 and MyD88 were decreased by serum deprivation. Although ROS production was decreased in TLR4-nonfunctional or MyD88-deficient splenocytes as compared to that in control mice, serum deprivation increased ROS production and augmented BAFF expression in both cells. $50{\mu}M\;H_2O_2$ also increased BAFF expression in TLR4-deficient or MyD88-deficient splenocytes. Collectively, results show that BAFF expression may be mediated by TLR4 or MyD88-independent manner and TLR4 or MyD88 may not be required in BAFF expression.