• Title/Summary/Keyword: oxygen production rate

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A Theoretical Consideration on Oxygen Production Rate in Microalgal Cultures

  • Kim, Nag-Jong;Lee, Choul-Gyun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.5
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    • pp.352-358
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    • 2001
  • Because algal cells are so efficient at absorbing incoming light energy, providing more light energy to photobioreactors would simply decrease energy conversion efficiency. Furthermore, the algal biomass productivity in photobioreactor is always proportional to the total photosynthetic rate. In order to optimize the productivity of algal photobioreactors (PBRs), the oxygen production rate should be estimated. Based on a simple model of light penetration depth and algal photosynthesis, the oxygen production rate in high-density microalgal cultures could be calculated. The estimated values and profiles of oxygen production rate by this model were found to be in accordance with the experimental data. Optimal parameters for PBR operations were also calculated using the model.

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Effect of Oxygen Transfer Rate and Dissolved Oxygen on the Production of PHBV by Azoto-bacter vinelandii UWD. (산소전달 속도와 용존산소가 Azotobacter vinelandii UWD의 생분해성 고분자(PHBV) 생산에 미치는 영향)

  • 박창호
    • Microbiology and Biotechnology Letters
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    • v.26 no.6
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    • pp.529-536
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    • 1998
  • In a 20 L fermentor experiments the level of dissolved oxygen (D.O.) strongly affected growth and PHBV production of Azotobacter vinelandii UWD. A higher D.O. (5%) increased specific cell growth rate two folds but PHBV production was 17 folds higher (62.3 wt%) at a lower D.O.(1%) level. D.O. level was not a good criterion to evaluate the effect of aeration on fermentation characteristics of A. vinelandii UWD. This strain maintained an equal D.O. (5%) by decreasing its oxygen consumption rate when oxygen transfer rate (OTR) was decreased by changing agitation speed at a fixed aeration rate. OTR rather than D.O. was a criterion to explain the effect of aeration on the cell growth and PHBV production. At 5% D.O. with a lower 0TR cell growth rate decreased but PHBV production (57.3 wt%) approached to that (62.3 wt%) of the lower (1%) D.O.

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Estimation of Dissolved Oxygen in Streams using Reaeration, 1st Production and Respiration Rates (재포기 계수, 1차 생산율 및 호흡률을 이용한 하천의 용존산소 추정)

  • Kim, Kyung-Sub;Hwang, Sung-Gyu
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.6
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    • pp.428-433
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    • 2009
  • Dissolved oxygen is considered as one of the important water-quality constituents in streams from one century ago and fishes perish in low dissolved oxygen concentration. Environmental scientists and engineers have introduced the deterministic model to estimate dissolved oxygen concentration of streams and recommended the use of the Delta Method (DM), Approximate Delta Method (ADM), Extreme Value Method (EVM) and Optimization Method (OPT) which can be applied in no spatial variation of dissolved oxygen. The diurnal or annual variation of dissolved oxygen is mainly determined from the parameters such as reaeration rate, 1st production rate and respiration rate which are related to dissolved oxygen. Each method was briefly introduced and applied to two sampling sites of Anseong Stream watershed in this paper. The limitation, advantages and disadvantages of each method were reviewed and analyzed after running the each method. From these analyses, the benefit-cost approach to estimate dissolved oxygen effectively in streams was recommended.

Effect of Dissolved Oxygen on the Growth of Azotobacter vinelandii UWD and Production of PHBV in the Mixture of Organic Acids and Glucose (유기산 및 포도당 혼합배지에서 Azotobacter vinelandii UWD의 생장 및 PHBV 생산에 대한 용존산소의 영향)

  • 박창호
    • KSBB Journal
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    • v.13 no.6
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    • pp.675-680
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    • 1998
  • In both 7L and 20L fermentor experiments the level of dissolved oxygen (D.O) strongly affected growth and PHBV production of Azotobacter vinelandii UWD. A higher D.O. increased carbon substrate consumption rate and cell growth rate with a similar residual biomass production. However, a lower D.O. was a much better condition for PHBV production. In a 20L fermentor experiments controlled at 5% D.O. cell growth rate was about twice faster(0.555 hr-1 and 0.260 hr-1 at the acid and the glucose phase, respectively) with an equal amount(4.5 g/L) of residual biomass production. However, PHBV content in the cell(62.3 wt%) increased 17.3 times at 1% D.O.

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Improved Poly-${\varepsilon}$-Lysine Biosynthesis Using Streptomyces noursei NRRL 5126 by Controlling Dissolved Oxygen During Fermentation

  • Bankar, Sandip B.;Singhal, Rekha S.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.6
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    • pp.652-658
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    • 2011
  • The growth kinetics of Streptomyces noursei NRRL 5126 was investigated under different aeration and agitation combinations in a 5.0 l stirred tank fermenter. Poly-${\varepsilon}$-lysine biosynthesis, cell mass formation, and glycerol utilization rates were affected markedly by both aeration and agitation. An agitation speed of 300 rpm and aeration rate at 2.0 vvm supported better yields of 1,622.81 mg/l with highest specific productivity of 15 mg/l.h. Fermentation kinetics performed under different aeration and agitation conditions showed poly- ${\varepsilon}$-lysine fermentation to be a growth-associated production. A constant DO at 40% in the growth phase and 20% in the production phase increased the poly-${\varepsilon}$-lysine yield as well as cell mass to their maximum values of 1,992.35 mg/l and 20.73 g/l, respectively. The oxygen transfer rate (OTR), oxygen utilization rate (OUR), and specific oxygen uptake rates ($qO_2$) in the fermentation broth increased in the growth phase and remained unchanged in the stationary phase.

Cultivation of Sprout by Highly Concentrated Oxygen Water Soaking (고농도 산소수 침지를 통한 새싹 재배)

  • Cha, Jin-Myeong;Hong, Seung-Ho;Kim, Sun-Yil;Park, Ju-Young;Kim, Maeng-Su;Lee, In-Hwa
    • KSBB Journal
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    • v.23 no.6
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    • pp.525-528
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    • 2008
  • In order to compare the germination and growth rate of the sprouts soaked in highly concentrated oxygen water, it with specific amounts of oxygen dissolved was produced in a high pressure reactor by pressuring oxygen. The sprouts were observed after being soaked in $20^{\circ}C$ oxygen water with 20, 30, 40, 50 ppm of oxygen dissolved each. Results of ten days later indicate that the final germination rate of the sprout soaked in 50 ppm oxygen water was $24.6{\sim}28.6%$ higher than that of the sprout soaked in distilled water. The final growth length also measured 6-7 mm higher than the sprout soaked in distilled water, demonstrating that enough supply of oxygen to the sprout induces stability and efficiency in its growth.

NOx Formation Characteristics with Oxygen Enrichment in Nonpremixed Counterflow and Coflow Jet Flames (비예혼합 대향류 및 동축 제트화염에서 산소부화에 따른 NOx 생성특성)

  • Yoo, Byung-Hun;Hwang, Chul-Hong;Han, Ji-Woong;Lee, Chang-Eon
    • 한국연소학회:학술대회논문집
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    • 2004.11a
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    • pp.169-174
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    • 2004
  • The NOx emission characteristics with oxygen enrichment in nonpremixed counterflow and coflow jet flame of $CH_4$ fuel have been investigated numerically. A small amount of nitrogen is included in oxygen-enriched combustion, in order to consider the inevitable $N_2$ contamination by air infiltration. The results show that the initial increase of NO with increasing oxygen enrichment is due to increasing temperature and residence time, while its subsequent decrease above 75% oxygen is due to decreasing the consumption rate of nitrogen. When oxygen addition exceeds 30%, Thermal NO gradually becomes the dominant production pathway and Prompt NO becomes negative pathway for net NO production rate. It is also seen that Thermal NO plays an important role in NO reduction when strain rate increase in oxygen-enriched combustion. Finally, the results of EINOx with oxygen enrichment in coflow jet flame show the similar profile with those of conterflow flame. It is confirmed that, with leakage of 1% nitrogen in the oxidizer stream, the corresponding EINOx is eight times of that emitted from regular $CH_4$/Air flame.

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Effects of Oxygen Supply and Mixed Sugar Concentration on ${\small{D}}$-Ribose Production by a Transketolase-Deficient Bacillus subtilis SPK1

  • Park, Yong-Cheol;Lee, Hae-Jin;Kim, Chang Sup;Seo, Jin-Ho
    • Journal of Microbiology and Biotechnology
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    • v.23 no.4
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    • pp.560-564
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    • 2013
  • ${\small{D}}$-Ribose is a value-added five-carbon sugar used for riboflavin production. To investigate the effects of oxygen supply and mixed sugar concentration on microbial production of ${\small{D}}$-ribose, a transketolase-deficient Bacillus subtilis SPK1 was cultured batch-wise using xylose and glucose. A change of agitation speed from 300 rpm to 600 rpm at 1 vvm of air supply increased both the xylose consumption rate and ${\small{D}}$-ribose production rate. Because the sum of the specific consumption rates for xylose and glucose was similar at all agitation speeds, metabolic preferences between xylose and glucose might depend on oxygen supply. Although B. subtilis SPK1 can take up xylose and glucose by the active transport mechanism, a high initial concentration of xylose and glucose was not beneficial for high ${\small{D}}$-ribose production.

Research of Biofuel Syngas Production Using Superadiabatic Compression Spark Ignition Reformer (초단열 압축스파크 점화개질기를 이용한 바이오 합성가스 생산 연구)

  • Lim, Mun-Sup;Chun, Young-Nam
    • Transactions of the Korean hydrogen and new energy society
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    • v.21 no.1
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    • pp.42-49
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    • 2010
  • Increasing environmental concerns regarding the use of fossil fuels and global wanning have prompted researcher to investigate alternative fuels. The purpose of this study is to investigate the syngas production by biogas reforming using a compression spark ignition engine. The parametric screening studies were carried out according to the variations of oxygen enrichment rate, biogas $CO_2$ ratio, intake gas temperature, and engine revolution. When the oxygen enrichment rate and input gas temperature increased, hydrogen and carbon monoxide were increased. But the biogas $CO_2$ ratio and engine revolution increased, the syngas were reduced. For the reforming of methane 100% only, generation of hydrogen and carbon monoxide was 58% and 17%, respectively. However when the biogas $CO_2$ ratio was 40%, hydrogen and carbon monoxide concentration were about 20% each.

The Oxygen Transfer and Oxygen Uptake in Antibiotic Fermentation using Streptomyces kanamyceticus (항생물질발효에서의 산소전달 및 흡수속도에 관한 연구)

  • Lee, Kye-Joon;Moo Bae
    • Microbiology and Biotechnology Letters
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    • v.10 no.3
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    • pp.223-226
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    • 1982
  • The aim of the present study was to assess the oxygen transfer rate and oxygen uptake rate in antibiotic fermentation. As a model study, cultures of Streptomyces kanamyceticus in a complex medium were analyzed to evaluate the oxygen transfer and uptake rates using oxygen balance technique. Quantitative evidence for the effect of oxygen transfer rate on the volumetric antibiotic production was clearly demonstrated. The oxygen uptake rates and the specific oxygen requirements were significantly changed with culture time. Those phenomena were indicative of biological turnover in the antibiotic fermentation.

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