Park Gi-Ho;Shin Hang-Sik;Park Min-Ho;Hong Seung-Mo;Ko Seok-Oh
Journal of Soil and Groundwater Environment
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v.10
no.4
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pp.45-53
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2005
The objective of this study is to evaluate the effects of changes in soil temperature on biodegradation rate of diesel compounds from a field pilot test using hot air injection process. Total remediation time was estimated from in-situ biodegradation rate and temperature for optimum biodegradation. All tests were conducted by measuring in-situ respiration rates every about 10 days on highly contaminated area where an accidental diesel release occurred. The applied remediation methods were hot air injection/extraction process to volatilize and extract diesel compounds followed by a bioremediation process to degrade residual diesels in soils. Oxygen consumption rate varied from 2.2 to 46.3%/day in the range of 26 to $60^{\circ}C$, and maximum $O_2$ consumption rate was observed at $32.0^{\circ}C$. Zero-order biodegradation rate estimated on the basis of oxygen consumption rates varied from 6.5 to 21.3 mg/kg-day, and the maximum biodegradation rate was observed at $32^{\circ}C$ as well. In other temperature range, the values were in the decreasing trend. The first-order kinetic constants (k) estimated from in-situ respiration rates measured periodically were 0.0027, 0.0013, and $0.0006d^{-1}$ at 32.8, 41.1, and $52.7^{\circ}C$, respectively. The estimated remediation time was from 2 to 9 years, provided that final TPH concentration in soils was set to 870 mg/kg.
The Yugeum deposit in Youngduk in Gyungsangbuk-do is emplaced in the Cretaceous granitoids located in the Northeastem Gyeongsang Basin. Gold-bearing quartz veins filling the fracture with a direction of $N19^{\circ}{\sim}38^{\circ}W$ are most abundantly distributed within the Younghae granodiorite body. The formation of quartz veins can be classified into three main stages: barren quartz stage, auriferous quartz vein stage, and finally the extensive sulfide mineralization stage. Various sulfide minerals such as pyrite, chalcopyrite, galena, sphalerite, and arsenopyrite were precipitated during the hydrothermal gold mineralization process. Gold commonly occurs as fine-grained electrum in sulfides with high Au concentration (up to 93 wt%) compared to Ag. During the early gold mineralization stage, the temperature and pressure of the fluids are in the range of $220{\sim}250^{\circ}C$ and 730~1800 bar, and the oxygen fugacity is between $10^{-27}$ and $10^{-31.7}$ atm. On the other hand, the fluids of the late stage mineralization are characterized by temperature of $290{\sim}350^{\circ}C$ and pressure of 206~472 bar, and the oxygen fugacity is in the range of $10^{-26.3}{\sim}10^{-28.6}$ atm. The sulfur isotope compositions of sulfide minerals are in the range of $0.2{\sim}4.2^{\circ}/_{\circ\circ}$, while the ${\delta}^{34}SH_2S$ values range from 1.0 to $3.7^{\circ}/_{\circ\circ}$. The Ag/Au atomic ratios of electrum ranges from 0.15 to 1.10, and Au content is higher than Ag in most electrum. During the main gold mineralization stage at the relatively high temperature condition and with pH from 4.5 to 5.5, the stability of ${AuCl_2}^-$ increased while the stability of ${Au(HS)_2}^-$ decreased. Considering the pressure estimated in this deposit, the temperature of the ore fluid reached higher than $350^{\circ}C$ and ${AuCl_2}^-$ became an important species for the gold transportation. As mineralization proceeded with decreasing temperature and increasing pH and $f_{o2}$, the precipitation of sulfide minerals and accompanying electrum occurred.
KANG, DONG-JIN;CHUNG, CHANG SOO;COOPER, LEE W.;KANG, CHEONG YOON;KIM, YEA DONG;HONG, GI HOON
한국해양학회지
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v.27
no.3
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pp.250-258
/
1992
The oxygen isotope composition of surface waters in the Bransfield Strait was determined as one extra state variable in order to characterize water masses in the region, since salinity is significantly modified due to the freezing and ice-melting in the polar region. The salinity, temperature, and $\delta$/SUP 18/O values vary from 34.0 to 34.5$\textperthousand$, -.05 to 2.1$^{\circ}C$ and -0.50 t -0.26$\textperthousand$, respectively. The combined effects of evaporation, precipitation, freezing, ice-melting are reflected in the widely scattered data. Although it is small, the distribution of $\delta$/SUP 18/O of the Bransfield Strait is strongly affected by the freezing-ice melting rather than the evaporation-precipitation. The ice melted fresh water which has higher temperature, depleted salinity and nutrients may be injected to the Bransfield Strait from the north. The concentrations of nutrients are decreasing gradually from the north to the south. The waters were characterized by two groups of higher (about 19.4) and lower N/P ratio (about 16.7). The lower N/P ratio is found in the northern part where ice-melted fresh water is injected. and the higher N/P ratio is found in the southern part of the Bransfield Strait. Although more precise work is needed, the deference of N/P ratio can be an evidence of the ice melted water injection to the Bransfield Strait. Chlorophyll a concentrations, in general, increase from northwest (Waddell Sea) to the southeast (Smith and Hosseason Islands). Probably the injection of nutrient depleted fresh water from the ice melting reduce the chlorophyll a concentration.
Kim, Min Yeong;HwangBo, Hyun;Ji, Seon Yeong;Hong, Su-Hyun;Choi, Sung Hyun;Kim, Sung Ok;Park, Cheol;Choi, Yung Hyun
Journal of Life Science
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v.29
no.4
/
pp.410-420
/
2019
Citrus unshiu peel extracts possess a variety of beneficial effects, and studies on their anticancer activity have been reported. However, the exact mechanisms underlying this activity remain unclear. In the current study, the apoptotic effect of ethanol extract of C. unshiu peel (EECU) on human breast adenocarcinoma MDA-MB-231 cells and related mechanisms were investigated. The results showed that the survival rate of MDA-MB-231 cells treated with EECU was significantly inhibited in a concentration-dependent manner, which was associated with the induction of apoptosis. EECU-induced apoptosis was associated with the activation of caspase-8 and caspase-9, which initiate extrinsic and intrinsic apoptosis pathways, respectively, and caspase-3, a representative effect caspase. EECU suppressed the expression of the inhibitor of apoptosis family of proteins, leading to an increased Bax/Bcl-2 ratio and proteolytic degradation of poly (ADP-ribose) polymerase. EECU also enhanced the loss of the mitochondrial membrane potential and cytochrome c release from the mitochondria to the cytosol, along with truncation of Bid. In addition, EECU activated AMP-activated protein kinase (AMPK), and compound C, an AMPK inhibitor, significantly weakened EECU-induced apoptosis and cell viability reduction. Furthermore, EECU promoted the generation of reactive oxygen species (ROS), which acted as upstream signals for AMPK activation as pretreatment of cells, with the antioxidant N-acetyl cysteine reversing both EECU-induced AMPK activation and apoptosis. Collectively, these findings suggest that EECU inhibits MDA-MB-231 adenocarcinoma cell proliferation by activating intrinsic and extrinsic apoptotic pathways, which was mediated through ROS/AMPK-dependent pathways.
Journal of the Korean Applied Science and Technology
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v.36
no.2
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pp.394-406
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2019
The cellular senescence may be due to damage by the reactive oxygen species (ROS). This study has compared the antioxidant activity in the human cell lines of various origins, including 10S and 50S-derived normal skin fibroblasts, and 10S bone marrow, dental tissue and adipose-derived adult stem cells. After being exposed to $H_2O_2$, half inhibitory concentration ($IC_{50}$) values by cytotoxicity assay was significantly (P<0.05) lower in 50S-derived skin fibroblasts, than in 10S-derived skin fibroblasts and various adult stem cell lines. The cell population doubling time (PDT) and the cell frequency with high senescence associated-${\beta}$-galactose activity were remarkably increased in 50S-derived fibroblasts exposed to 50 ppm $H_2O_2$ for 7 days, than those of 10S-derived fibroblasts and various adult stem cell lines. Further, the expression level of antioxidant-related genes, glutathione peroxidase (GPX) and catalase (CAT), was investigated in 10S and 50S-derived skin fibroblasts, and 10S-derived various adult stem cells by reverse transcription polymerase chain reaction (RT-PCR). The expression level of GPX was higher in most of cell lines, compared to CAT, and a significantly (P<0.05) higher expression level of GPX was observed in 10S-derived skin fibroblasts and adult stem cell lines, compared to 50S-derived skin fibroblasts. We concluded that old-aged skin fibroblasts seemed to be less resistant against ROS than young-aged skin fibroblasts and adult stem cells.
Since the construction of a dike in 1983, the water quality in the Bunam Lake has continued to deteriorate due to algal bloom caused by agricultural nutrient loading. Therefore, we evaluated the change in water quality and phytoplankton ecological characteristics in Bunam Lake and Cheonsu Bay, Korea. Water temperature, salinity, dissolved oxygen, chemical oxygen demand (COD), chlorophyll, and phytoplankton community were surveyed in April during the dry season and in July during the rainy reason. As a result, during the dry period, phytoplankton proliferated greatly and stagnated in the Bunam Lake while a very high population of cyanobacteria Oscillatoria spp. (8.61×107 cells L-1) was recorded. Most of the nutrients, except, nitrate and nitrite, were consumed due to the large growth of phytoplankton. However, during the rainy period, concentrations of ammonia, phosphate, silicate, nitrate, and nitrite, were very high towards the upper station due to the inflow of fresh water. Cyanobacteria Oscillatoria and Microcystis spp. were dominant in the Bunam Lake during the rainy period. Even in the Cheonsu Bay, cyanobacteria dominated due to the effect of discharge and diatoms, such as, Chaetoceros spp. and Eucampia zodiacus, which also proliferated significantly due to increased levels of nutrients. Since the eutrophication index was above 1 in Bunam Lake, it was classified as eutrophic water and the Cheonsu Bay was classified as eutrophic water only during the rainy season. In addition, a stagnant seawater-derived hypoxia water mass was observed at a depth of8m in the Bunam Lake adjacent to the tide embankment and the COD concentration reached 206 mg L-1 in the bottom layer at B3. Based on this result, it is considered that the water quality will continue to deteriorate if organic matters settle due to continuous inflow of nutrients and growth of organisms while the bottom water mass is stagnant.
In this study, an electronics industrial wastewater activated sludge model (e-ASM) to be used as a Water Digital Twin was calibrated based on real high-tech electronics industrial wastewater treatment measurements from lab-scale and pilot-scale reactors, and examined for its treatment performance, effluent quality prediction, and optimal process selection. For specialized modeling of a high-tech electronics industrial wastewater treatment system, the kinetic parameters of the e-ASM were identified by a sensitivity analysis and calibrated by the multiple response surface method (MRS). The calibrated e-ASM showed a high compatibility of more than 90% with the experimental data from the lab-scale and pilot-scale processes. Four electronics industrial wastewater treatment processes-MLE, A2/O, 4-stage MLE-MBR, and Bardenpo-MBR-were implemented with the proposed Water Digital Twin to compare their removal efficiencies according to various electronics industrial wastewater characteristics. Bardenpo-MBR stably removed more than 90% of the chemical oxygen demand (COD) and showed the highest nitrogen removal efficiency. Furthermore, a high concentration of 1,800 mg L-1 T MAH influent could be 98% removed when the HRT of the Bardenpho-MBR process was more than 3 days. Hence, it is expected that the e-ASM in this study can be used as a Water Digital Twin platform with high compatibility in a variety of situations, including plant optimization, Water AI, and the selection of best available technology (BAT) for a sustainable high-tech electronics industry.
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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v.12
no.4
/
pp.287-304
/
2007
This study was carried out to investigate spatio-temporal variations of benthic environment and macrobenthic polychaete communities in Gamak Bay where excessive organic matters from untreated sewage effluents and fish and shellfish farming activities have been accumulated in certain regions. Such environmental variables as sediment composition, organic content, acid volatile sulfide and dissolved oxygen content etc. were measured and polychaete specimens were taken in September 1999 and February 2000. In September 1999, organic contents were up to three times higher in the northwestern part of the bay and the area closed to Gukdong harbor than in the rest of the bay. In particular, benthic environment of the northwestern part of the bay was extremely deteriorated by the occurrence of hypoxia and highly concentrated sulfide. Of 28 stations investigated in summer, 5 stations located in the northwestern part were azoic. In the rest of the stations, a total of 119 polychaete species were sampled with a mean density of 900 $ind./m^2$. While species richness was higher in the entrance and central part of the bay where water exchanges with open sea were relatively active, density was higher in the moderately enriched stations neighbouring Gukdong harbor and fish farms where such potential indicators of organic pollution as Aphelochaeta monilaris, Lumbrineris longifolia were largely dominant. In February 2000, a total of 81 polychaete species appeared with a mean density of 2,802 $ind./m^2$ from 12 stations sampled in winter. Azoic areas were recolonized by Capitella capitata, Pseudopolydora paucibranchiata and Dipolydora socialis which showed maximum density in the innermost station. On the other hand, the rest of the stations were dominated by Euchone alicaudata and Praxillella affinis. The configuration of the stations sampled in summer and winter on the multidimensional scaling plots reflected the position of each stations within the bay in which there were great differences in organic content, sulfide concentration and oxygen content rather than in sediment composition. Therefore, heterogeneities in polychaete community structure in Gamak Bay were primarily influenced by the degree of the disturbance depending on the position within the bay.
Journal of the Korean Society for Marine Environment & Energy
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v.18
no.1
/
pp.1-8
/
2015
To evaluate accuracy and precision of determination of total alkalinity ($Alk_T$) and carbon dioxide ($TCO_2$) derived from present study, experiment was applied with $CO_2$ CRM (Batch 132, Scripps Institution of Oceanography; $Alk_T=2229.24{\pm}0.39{\mu}mol/kg$, $TCO_2=2032.65{\pm}0.45{\mu}mol/kg$). As the result, average concentration of $Alk_T$ and $TCO_2$ was $2354.09{\mu}mol/kg$ (~5.6% difference with $CO_2$ CRM) and $2089.60{\mu}mol/kg$ (~2.3% difference with $CO_2$ CRM), respectively. For previous method (Gran Titration) by addition $NaHCO_3$ to deionized water($Alk_T$$2023.33{\mu}mol/kg$), average concentration was $2193.39{\mu}mol/kg$ (sd=57.15, n=7). Whereas, average concentration was $2017.02{\mu}mol/kg$ (sd=10.98, n=7) for the present study. Recovery yield experiments of total alkalinity in deionized water and seawater were implemented by addition of $NaHCO_3$. The recovery yield of deionized water in the range 0 to $4952.39{\mu}mol/kg$ was 100.8% ($R^2$=0.999), and seawater in the range 0 to $2041.32{\mu}mol/kg$ was 102.3% ($R^2$=0.999). Comparison of $pCO_2$ sensor (PSI $CO_2-Pro^{TM}$) with present method showed very meaningful correlation coefficient ($R^2$=0.977) in the range of 427 to $705{\mu}atm$ and 9.16 to $15.24{\mu}mol/kg$ throught elapsed time for two weeks. Field experiment of diurnal variation of total carbon dioxide was accomplished at Sachon harbor in the coastal waters of East Sea of Korea. Concentration of $Alk_T$ and $TCO_2$ was increased during night, and decreased during daylight hours. The results showed mirror type between $TCO_2$ and dissolved oxygen, which was attributable to photosynthesis and respiration of phytoplankton. Also, open ocean field study was performed to obtain vertical profile of $Alk_T$ and $TCO_2$ in C-C zone (Clarion-Clipperton Fracture Zone), Northeastern Pacific. Average concentrations of $Alk_T$ in the surface mixed layer (0~60 m) and deeper layer below 200 m were $2422.38{\mu}mol/kg$ (sd=78.73, n=20) and $2465.87{\mu}mol/kg$ (sd=57.68, n=103), respectively. And average concentrations of $TCO_2$ were $2134.47{\mu}mol/kg$ (sd=65.4, n=20) and $2431.87{\mu}mol/kg$ (sd=65.02, n=103) in the same depth ranges such as $Alk_T$. Vertical distributions of $Alk_T$ and $TCO_2$ concentrations tended to increase with depth, and analyzed concentrations showed slightly higher than those of previous studies in this area.
Groundwater from public wells was monitored during one year with two month interval for hydrogeochemical parameters and chlorofluorocarbons (CFCs) as environmental tracers in Jeju Island. Concentrations of major cations and $SiO_2$ show variation less than 10% whereas $NO_3$ and dissolved oxygen (DO) showed larger variation though DO variation did not change oxic or suboxic condition. $NO_3$ concentration has no consistent seasonal pattern with the largest variation of 35%. Groundwater ages determined by CFCs became temporarily younger by 5 years in October for groundwater with ages of 15 to 25 years, which can be attributed to infiltrating water in rainy season. Compared to air temperature, groundwater temperature has much smaller variation with no phase difference, which can be accounted for by a two-component model consisting of infiltrating water from surface and deeper groundwater with negligible temperature variation. The relatively small variation in groundwater age and temperature indicates that groundwater recharge through fast flow-paths is much smaller compared with basal groundwater in terms of aquifer storage.
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