• BMB Reports
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• v.34 no.6
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• pp.551-558
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• 2001

We extended the measurable time scale of DNA dynamics to submicrosecond using a long-lifetime metal-ligand complex, $[Ru(phen)_2(dppz)]^{2+}$ (phen=1,10-phenanthroline, dppz=dipyrido[3,2-a:2',3'-c]phenazine) (RuPD), which displays a mean lifetime near 350 ns. We partially characterized the fluorescence resonance energy transfer (FRET) in calf thymus DNA from RuPD to nile blue (NB) using frequency-domain fluorometry with a high-intensity, blue light-emitting diode (LED) as the modulated light source. There was a significant overlap of the emission spectrum of the donor RuPD with the absorption spectrum of the acceptor NB. The F$\ddot{o}$rster distance ($R_0$) that was calculated from the spectral overlap was $33.4\;{\AA}$. We observed dramatic decreases in the steady-state fluorescence intensities of RuPD when the NB concentration was increased. The intensity decays of RuPD were matched the closest by a triple exponential decay. The mean decay time of RuPD in the absence of the acceptor NB was 350.7 ns. In a concentration-dependent manner, RuPD showed rapid intensity decay times upon adding NB. The mean decay time decreased to 184.6 ns at $100\;{\mu}M$ NB. The FRET efficiency values that are calculated from the mean decay times increased from 0.107 at $20\;{\mu}M$ NB to 0.474 at $100\;{\mu}M$ NB concentration. The use of FRET with a long-lifetime metal-ligand complex donor is expected to offer the opportunity to increase the information about the structure and dynamics of nucleic acids.

• Journal of KIISE:Databases
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• v.30 no.1
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• pp.52-63
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• 2003

Spatiotemporal moving objects are changing their Positions and/or shape over time in real world. As most of the indices of moving object are based on the R-tree. they have defects of the R-tree which are dead space and overlap. Some of the indices amplify the defects of the R-tree. In the paper, to solve the problems, we propose the MPR-tree(Moving Point R-tree) using Projection operation which has more effective search than existing moving point indices on time slice query and spatiotemporal range query. The MPR-tree connects positions of the same moving objects over time by using linked list, so it processes the combined query about trajectory effectively. The usefulness of the Projection operation is confirmed during processing moving object queries and in practical use of space from experimentation to compare MPR-tree with existing indices of moving objects. The proposed MPR-tree would be useful in the LBS, the car management using GPS, and the navigation system.

• Tunnel and Underground Space
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• v.9 no.1
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• pp.36-47
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• 1999

For cautious controlled blasting, it is necessary to understand characteristics of the blasting vibration. In this study, a series of tests were carried out to investigate the several characteristics of blasting vibration waveform by vibration time history analysis. Separation between impulse vibration and free vibration from blasting vibration, duration time, effects of overlap of free vibration upon the level of vibration and changes of waveform according to increase of charge weight per delay etc. were studied with waveform analysis.

• Journal of the Korean Institute of Landscape Architecture
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• v.28 no.2
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• pp.1-9
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• 2000

The purpose of this study is to examine influence of interior landscape in commercial spaces on the management benefit. In the course of this study, objects of survey were selected from interior landscaping specialist, manager and consumer who had experiences to visit to the hotel, bank, department store and restaurant. The main method of data collection was interview, questions and gathering materials. The cronbach's alphas program was used to measure the reliability of likert scales. The analysis program was applied a statistical methods. The results of this study can be summarized as follows: The factors considered by consumer who visits to the commercial space are design, interior landscape, traffic, parking capacity, cleanness, price, kindness of employee and event. Factors concerned with the product include branch size, traffic, parking capacity, kindness of employee. Factors concerned with facilities include branch size, traffic, parking capacity, kindness of employee. Major factors of those effects to management benefit are accessibility, time of stay, sales, efficiency of space, attractiveness and difference. In conclusion, overlap style gets the highest degree of satisfaction among total factors. Results of the survey show that factors which improve sales are overlap style and planter type. Among four commercial spaces, hotel is most effected by interior landscape. To enhance the sales of hotel, further study of interior landscape about hotel necessary. For bank and restaurant, further study of economic planter type and style is necessary. For department store, optimal use of spaces and case of maintenance are necessary.

• Transactions of the Korean Society of Automotive Engineers
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• v.8 no.4
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• pp.26-33
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• 2000

In-cylinde flow and mixture formation are key contributors to both idle stability and combustion stability at part load condition in SI engine. The real time measurements of air-fuel ration and in- cylinder residual gas fraction are particularly important to obtain a better understanding of the mechanisms for combustion and emissions especially during cold start and throttle transient condition. This paper reports the cycle resolved measurements of residual gas fraction and equivalence ration near speak plug with value timing change and their effects on combustion characteristics at part load. The results showed that the effect of intake value opening on the residual gas fraction was smaller than that of exhaust valve closing because of the decreases of exhaust gas reverse flow from exhaust port. The variation of equivalence ratio near spark plug increased with the increase of value overlap and it closely related with heat release rate and combustion stability

• Journal of the Korean Institute of Rural Architecture
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• v.23 no.3
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• pp.19-26
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• 2021

This study conducted an analysis of the distribution and status of baby boomer households and trends of turn to farming and returning home. At the same time, the density analysis of land price increase data, building data, and floating population data was conducted in Jeollanam-do County. The established base areas were superimposed to distinguish the regional centers according to the number of superimposed indicators. It is intended to present management measures according to the degree of activation of the center. An area where three bases overlap can be designated as a center activation area, and a place where two regions overlap can be designated as a center semi-activation area. Using the analysis of regional centers at the county level, hierarchies of the centers were classified and management measures were presented. Based on these findings, this study addresses meaningful applicability for regional and medium- and long-term plans.

• Proceedings of the IEEK Conference
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• 2001.06d
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• pp.155-158
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• 2001

A new method for simultaneous multiple transmit focusing using orthogonal weighted FM chirp is proposed. Weighted chirp signals focused at different depths are transmitted at the same time. These chirp signals are mutually orthogonal in the approximate sense that the autocorrelation function of each signal has a narrow mainlobe width and low sidelobe levels, and the crosscorrellation function of any pair of the signals has smaller values than the sidelobe levels of each autocorrelation function. This means that each weighted chirp signal can be separately compressed into a short pulse, focused individually and combined with other focused beams to form a frame of image. Theoretically, any two chirp signals defined in two nonoverlapped frequency bands are mutually orthogonal. In the present work, however, a fractional overlap of adjacent frequency bands, by up to 25%, were permitted to design more chirp signals within a given transducer bandwidth. The crosscorrelation values due to the frequency overlap could be reduced by alternating the direction of frequency sweep of the adjacent chirp signals. The simulation results show that this method can improve the lateral resolution of image without sacrifice in the frame rate compared with the conventional pulse system.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.11
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• pp.5419-5435
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• 2017

The construction of panoramic images on smartphones and low-powered devices is a challenging task. In this paper, we propose a new approach for smoothly stitching images on mobile phones in the presence of moving objects in the scene. Our main contributions include handling moving object problems, reducing processing time, and generating rectangular panoramic images. First, unique and robust feature points are extracted using fast ORB method and a feature matching technique is applied to match the extracted feature points. After obtaining good matched feature points, we employ the non-deterministic RANSAC algorithm to discard wrong matches, and the hommography transformation matrix parameters are estimated with the algorithm. Afterward, we determine precise overlap regions of neighboring images and calculate their absolute differences. Then, thresholding operation and noise removal filtering are applied to create a mask of possible moving object regions. Sequentially, an optimal seam is estimated using dynamic programming algorithm, and a combination of linear blending with the mask information is applied to avoid seam transition and ghosting artifacts. Finally, image-cropping operation is utilized to obtain a rectangular boundary image from the stitched image. Experiments demonstrate that our method is able to produce panoramic images quickly despite the existence of moving objects.

• Korean Journal of Computational Design and Engineering
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• v.21 no.3
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• pp.296-305
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• 2016

This paper proposes a framework to automatically align Dental range image captured by depth sensors like the Microsoft Kinect. Aligning dental images by intraoral scanning technology is a difficult problem for applications requiring accurate model of dental-scan datasets with efficiency in computation time. The most important thing in dental scanning system is accuracy of the dental prosthesis. Previous approaches in intraoral scanning uses a Z-buffer ICP algorithm for fast registration, but it is relatively not accurate and it may cause cumulative errors. This paper proposes additional Alignment using the rough result comes after intraoral scanning alignment. It requires that Each Depth Image of the total set shares some overlap with at least one other Depth image. This research implements the automatically additional alignment system that aligns all depth images into Completed model by computing a network of pairwise registrations. The order of the each individual transformation is derived from a global network and AABB box overlap detection methods.

• The Journal of the Korea Contents Association
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• v.12 no.2
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• pp.1-9
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• 2012

This paper presents an algorithm for depression diagnosis using the Neural Network with Weighted Fuzzy Membership functions (NEWFM) and heart rate variability (HRV). In the algorithm, 22 different features were initially extracted from the HRV signal by frequency domain, time domain, wavelet transformed, and Poincar$\acute{e}$ transformed feature extraction methods; of these 6 optimal features were selected by significance evaluation using Non-overlap Area Distribution Measurement (NADM) based on NEWFM. The proposed algorithm uses these 6 optimal features to diagnose depression with an accuracy of 95.83%.