• 생약학회지
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• 제28권4호
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• pp.264-270
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• 1997

This Study was carried out develop antitumor effect of the n-butanol soluble of fraction of Perilla frutescens on human skin melanoma cells. The antitumor activity of various fractions obtained form n-butanol soluble fraction of Perilla frutescens was evaluated in human skin melanoma cells. The antitumor activity of the n-butanol soluble fraction in human skin melanoma cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, neutral red (NR) assay and sulforhordamine B protein (SRB) assay of colorimetic assay methods. The light microscopic study was carried out to observe morphological changes of cultured human skin melanoma cells. These results were obtained follows; The fractions 5 and 6 of the n-butanol soluble fraction of P frutescens were shown significant antitumor activities. The number of human skin melanoma cells were decreased and tend to form cell cluster by treatment with actions 5 and 7 of the n-butanol soluble fraction of P. frutescens. The fraction 6 of the the n-butanol soluble fraction showed the highest antitumor activity on P. frutescens. These results suggest that the fraction 6 of the n-butanol soluble fraction of P. frutescens may be a valuable choice for the studies on the treatment of human skin tumors.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.178.1-178.1
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• 2003

This study was carried out to evaluate cytotoxic effects of Houttuynia cordata THUNB extracts on A549 (lung cancer), MDA-MB231 (breast cancer), SNU-C4 (colon cancer) and B16 (mouse melanoma) cell lines. We have determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The 150 $\mu$g/$m\ell$ concentration of methanol extract (63.81 ％) of Houttuynia cordata THUNB was shown significantly antitoxic activity on A549 cell lines. (omitted)

• 한국환경교육학회지:환경교육
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• 제22권3호
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• pp.83-96
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• 2009

This article extracted feasible elements for an environmental education program from the 'Intelligent Life', an integrated subject of the 7th elementary school curriculum, and designed a program for second graders in the elementary school and applied it to them in order to examine how the students' environmental altitudes changed. In the controlled class, the instruction followed the lessons specified in the 7th national curriculum faithfully while for the experimental class 1, only the inquiry activity-based environmental education program was applied. Moreover, in experimental class 2, the inquiry activity-based environmental education program was implemented and wrap-up activity was performed as well with the products. To verify the program, questionnaires on the change of environmental altitudes were distributed and analyzed total three times in the pretest, posttest 1(right after the program), and posttest 2(30 days after the program). T-test was carried out with the SPSS 12 program to verify the effect of the applied program. To make up for the quantitative analysis, qualitative analysis was also conducted. The followings show the results gained after the program was implemented. First, This program showed a significant difference(p<.05) in the students' formation of environmental altitudes. Second, it was verified that the inquiry activity-based environmental education class that reorganized the second grade elementary students' 'Intelligent Life' subject had a more positive effect in the cultivation of environmental altitude than the class that followed the present curriculum as it was. It will be necessary to perform follow-up researches on the relativity between curricula for kindergarteners and lower grade elementary school students.

• 한국학교ㆍ지역보건교육학회지
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• 제16권2호
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• pp.101-114
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• 2015

Objectives: Find out how it affects anxiety situations and self-esteem targeting the critical period of development of self-esteem in the lower grades in elementary school based on laughter therapy in having a positive influence on physical health promotion and positive emotional cultivation. And to verify the effects of laughter activity was to provide preliminary data on mental health education and guidance of school life. Methods: By targeting Ulsan Y elementary school, second grade 7 class, 191 students conducted test self-esteem and anxiety. Homogeneous group of experimental and control groups were selected in each one class. Classified as experimental group carried out laughter activity programs and control group did not carried out. Also the separation of the application program before and after, then it were performed preliminary-post-further as same checklist. Program coverage period is 12 weeks and 30 minutes per session was conducted twice a week. Differences between the experimental group and the comparison group has conducted the independent samples t-test, and if the difference between the experimental group and the comparison group were verified by performing a variance analysis(Oneway ANOVA) and post multiple comparisons. Results: Analysis of the experimental group decreased anxiety in the post-test than the pre-test as a whole, in particular, sub-areas of concern, shame and shyness was shown to be effective, and decision of the difficulty was not significant by insignificant reduction. In addition, self-esteem was increased in overall score. The score of collective self-esteem and School life self-esteem were more increased, and domestic self-esteem was not significant by slight increases. In contrast, the comparison group showed no significant difference in all areas of anxiety and self-esteem of pre-test and post-test. Conclusions: In experiments on self-esteem and anxiety of lower grade in elementary school through laughter activity program, comparison group it did not show an effect by accident, and experimental group showed meaningful effects that self-esteem was increased and anxiety was reduced. Thus, laughter activity can be seen that improving self-esteem, anxiety reduction and etc, is very beneficial to mental health.

• 한국초등과학교육학회지:초등과학교육
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• 제25권spc5호
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• pp.522-531
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• 2007

A Science Camp Program was developed and applied as an intensified course for gifted students. The implications for the development and implementation of out-of-school science activities were also deduced through the analysis of the preliminary application results. The key point of the science camp program is to boost students' science inquiry skills through self-directed activities. Several positive effects in terms of interest and participation in the program were observed and some implications were derived as follows; (1) The program should provide the students with more opportunities for discussion and debate in group activities. (2) The tasks need be divided into two parts; basic tasks and optional tasks in order to ensure that the students engage in fewer tasks more intensively. (3) Each activity needs sufficient orientation taking consideration of the possibility that not all students may be ready for the inquiry. (4) The use of real examples of scientific research processes can help the students develop open inquiry skills and problem posing skills.

• 한국과학교육학회지
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• 제24권1호
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• pp.157-170
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• 2004

Students experience science not only through school science lessons but also through various other channels. Science-related facilities, including science museums and centers, are important channels for informal science education. Korea has a number of governmental and private facilities in which young people can experience various aspects of scientific activities, and many of them also provide the programs supporting out-of-school science activities. However, there has been no systematic survey study on those facilities providing out-of-school activities, thus they have not been used effectively as well as their social utility is not fully realized. To this end, in 2002 researchers of this study carried out surveys (1) of the facilities supporting out-of-school science activities and (2) of science programs provided by these facilities. The surveys show that there are approximately 180 facilities supporting science activities in Korea. More than 40% of them are located in Seoul and Gyeonggi areas. Among them, the proportion of special theme science museums was the greatest (37.9%). The facilities supporting out-of-school science activities usually do not target the specific age groups but are intended for all people. The proportion of governmental facilities exceeds that of private ones. 41.8% of the facilities examined in this study run their own science activity programs. Among the 10 categories of the programs, 'science class' type programs were the most common. There were more programs for elementary and middle school students than preschoolers, high school students and adults. The contents of the programs were more related to astronomy and meteorology, the observation of living things and field trips. Despite their high practical potential, the special theme science museums were found to be lacking in relevant programs, which could fulfill their values for informal science education.

• Journal of Microbiology and Biotechnology
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• 제8권6호
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• pp.700-704
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• 1998

Each of the recombinant structural genes of Helicobacter pylori urease, ureA and ureB, was cloned and overexpressed as inclusion bodies. Solubilization and renaturation of the inclusion bodies were carried out, to accelerate the pairing of sulfhydryl groups and the incorporation of nickel ions, which would lead to the native structure with high enzyme activity. Rates of urea hydrolysis were monitored as an indication of in vitro activation of renatured ureases. The activation of the apoprotein using 1 mM nickel ion, 100 mM sodium bicarbonate and a 10:1 ratio of reducing power resulted in a weak urease activity (about 11% of the native urease activity encoded by pTZ 19R/ure-l). When a sparse matrix screen method originally discovered for the crystallization of proteins was used, the activity increased higher than that obtained using glutathione. The effect of polyethylene glycol (PEG) on the activity was noticeable, giving two-fold increase in the specific activity (about 11 U/mg of protein corresponding to 22% of the native urease activity encoded by pTZ19R/ure-1).

• 한국분말재료학회지
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• 제18권6호
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• pp.538-545
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• 2011

This study shows that catalytic activity of bimetallic RhPt nanoparticle arrays under CO oxidation can be tuned by varying the size and composition of nanoparticles. The tuning of size of RhPt nanoparticles was achieved by changing concentration of rhodium and platinum precursors in one-step polyol synthesis. Two-dimensional RhPt bimetallic nanoparticle arrays in different size and composition were prepared through Langmuir-Blodgett thin film technique. CO oxidation was carried out on these two-dimensional nanoparticle arrays, revealing higher activity on the smaller nanoparticles compared to the bigger nanoparticles. X-ray photoelectron spectroscopy (XPS) results indicate the preferential surface segregation of Rh compared to Pt on the smaller nanoparticles, which is consistent with the thermodynamic analysis. Because the catalytic activity is associated with differences in the rates of $O_2$ dissociative adsorption between Pt and Rh, this paper suppose that the surface segregation of Rh on the smaller bimetallic nanoparticles is responsible for the higher catalytic activity in CO oxidation. This result suggests a control mechanism of catalytic activity via synthetic approaches of colloid nanoparticles, with possible application in rational design of nanocatalysts.

• 한국사회복지학
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• 제68권3호
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• pp.105-125
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• 2016

학교결석은 학업중단이나 비행 범죄에 대한 잠정적인 조기 예측요인으로 그 중요성이 강조되고 있다. 이에 본 연구는 생태체계적 관점을 토대로 시간의 흐름에 따라 아동의 학교결석일에 영향을 미치는 다양한 보호요인과 위험요인을 경험적으로 검증하였다. 이를 위해 한국아동 청소년패널조사에서 초4 패널의 2차년도와 3차년도의 자료를 활용하였으며, 초등학교 2,378명을 대상으로 패널회귀분석의 고정효과모형을 적용하였다. 분석결과, 아동의 컴퓨터 사용시간, 부모의 학대, 학교활동참여정도, 학업성적이 학교결석일 변화에 중요한 영향을 미치는 것으로 나타났다. 특히 컴퓨터 사용시간, 부모의 학대는 학교결석에 위험요인으로 작용하였으며, 학교활동참여와 학업성적은 학교결석에 보호요인으로 작용하였다. 이러한 연구결과들을 바탕으로 우리나라 아동 청소년 문제에 대한 예방적 접근의 필요성을 강조하고 학교결석에 대한 체계적인 모니터링 시스템 구축의 필요성을 제언하였다.

• 한국수학교육학회지시리즈A:수학교육
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• 제43권4호
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• pp.419-440
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• 2004

The purpose of this paper is to know the effects on number and operation abilities of the 1st grade children of elementary school by applying teaching and learning activity throught communication. For this purpose, we have studied according to the following procedure. 1. We divised teaching and learning model through communication and applied in the actual teaching and learning activity. 2. We investigated the effects of number and operations abilities of the 1st grade children by applying teaching and learning activity through communication. To accomplish this purpose, we applied learning activity through communication to the 1st grade of 40 elementary school children for about six months(September 1, 1999 ～ February 20, 2000). In process of applying this model, we collected all sorts of cases in the children's learning activity and investigated children's response on learning activity through communication, interview with children and researcher's observation. We applied the model through communication in class and compared with the traditional learning. 1. In learning through communication, children could solve the problem in themselves with a sense of responsibility. 2. It was impossible to find out the degree of children's comprehension in the explanatory learning. But in the learning through communication, it was a great help to individualize and plan the learning because children express their ideas clearly. It has conclusion as follows. The learning activity through communication has effected on forming number and operations abilities of the 1st grade of elementary school children importantly. 1. Children have improved in the abilities through communication to express their own ideas. 2. Children have studied with a sense of responsibility not in the teacher-oriented learning but in the self-directed learning 3. Children could find out the mathematical concepts in themselves - correcting false concepts, reguiding concepts by errors, finding invisible errors, solving problems variously and knowing the easy method. 4. The activity through communication in mathematics was a base of children's individual learning and important data of next learning plan. 5. The mathematical concepts formed through communication had a high transfer of learning. 6. Children have taken pleasure of discovery and had affirmative attitude about mathematics learning. We can make sure that number and operations abilities of the 1st grade children are formed by applying teaching and learning activity through communication. However, help and control of teacher have to be with it.