• 제목/요약/키워드: oryza sativa

검색결과 919건 처리시간 0.039초

Black Rice (Oryza sativa L. var. japonica) Hydrolyzed Peptides Induce Expression of Hyaluronan Synthase 2 Gene in HaCaT Keratinocytes

  • Sim, Gwan-Sub;Lee, Dong-Hwan;Kim, Jin-Hwa;An, Sung-Kwan;Choe, Tae-Boo;Kwon, Tae-Jong;Pyo, Hyeong-Bae;Lee, Bum-Chun
    • Journal of Microbiology and Biotechnology
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    • 제17권2호
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    • pp.271-279
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    • 2007

  • Black rice (Oryza sativa L. var. japonica) has been used in folk medicine in Asia. To understand the effects of black rice hydrolyzed peptides (BRP) from germinated black rice, we assessed the expression levels of about 20,000 transcripts in BRP-treated HaCaT keratinocytes using human 1A oligo microarray analysis. As a result, the BRP treatment showed a differential expression ratio of more than 2-fold: 745 were activated and 1,011 were repressed. One of the most interesting findings was a 2-fold increase in hyaluronan synthase 2 (HAS2) gene expression by BRP. Semiquantitative RT-PCR showed that BRP increased HAS2 mRNA in dose-dependent manners. ELISA showed that BRP effectively increased hyaluronan (HA) production in HaCaT keratinocytes.

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Fingerprinting of Rice Genomes Using PCR with Arbitrary Primers

  • Park, Kyong-Hee
    • Preventive Nutrition and Food Science
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    • 제3권2호
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    • pp.198-202
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    • 1998

  • The arbitrary primed polymerase chain reaction (AP-PCR) has been used to detect the genetic alternations in the related species. Simple and reproducible fingerprints of complex genomes can be generated using single arbitrary chosen primers and the PCR. The technique was applied to the Oryza species and characterized the relationship among three cultivars of rice species based on theresult of genomic DNA fingerprints. The results indicated that the polymorphism revealed in rice strains and the differences in the PCR product pattern could be represented for each strainis. There was many variationsin the PCR product pattern between cv. Dongin(japonica type)and cv.Hyangdo (indica type), and our chosen AP-primers can ge as markers for strain identification and verfication.

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벼(Oryza sativa L.)의 지상부로부터 adenosine과 phlomuroside의 분리 및 동정 (Isolation and Identification of Adenosine and Phlomuroside from the Aerial Parts of Oryza sativa L.)

  • 정락훈;이대영;조진경;백윤수;서경화;이동걸;강희철;김지영;백남인
    • Journal of Applied Biological Chemistry
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    • 제57권4호
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    • pp.321-324
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    • 2014

  • 벼 지상부를 MeOH 수용액으로 추출하고 이 추출물을 n-hexane, EtOAc, n-BuOH, 및 $H_2O$ 분획으로 나누었다. n-BuOH 분획에 대하여 silica gel, ODS 및 Sephadex LH-20 column chromatography를 반복실시 하여 2종의 화합물을 분리하였다. NMR, IR 및 MS data를 해석하여 각각 adenosine (1)과 phlomuroside (2)로 구조 동정 하였다. 이들 화합물들은 벼에서는 처음으로 분리되었다.

  • https://doi.org/10.3839/jabc.2014.050 인용 PDF KSCI

캘러스 활용도를 향상시키기 위한 벼(Oryza sativa L.) 형질전환 시스템 구축 (Development of rice(Oryza sativa L.) transformation system to improve callus utilization)

  • 박지선;문기범;하장호;장지영;김미진;전재흥;박상언;김현순
    • 한국육종학회지
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    • 제49권3호
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    • pp.170-179
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    • 2017

  • 분자농업은 식물을 일종의 공장개념으로 확대 적용하여 산업적으로 가치가 높은 유용물질들을 대량생산하는 분야로 최근 많은 각광을 받고 있다. 그 중에서 벼 캘러스를 이용한 단백질 발현 시스템은 대량 배양이 가능하고 목적 단백질의 높은 발현율로 인한 산업화가 가능한 기술이다. 본 연구는 이러한 벼(Oryza sativa L.) 캘러스의 활용도를 높이기 위한 효율적인 형질전환 시스템을 구축하기 위해 수행되었다. 형질전환에 이용된 종자의 종피 제거 시 손을 이용함으로써 캘러스 유도율을 높여주었고, 6년 정도의 오래된 종자에서도 원활한 캘러스 유도가 가능하였다. 목적 유전자가 도입된 캘러스의 선발은 최소한 3주 이상의 배양기간을 필요로 하였고 가장 효율적인 것은 한번의 계대를 포함한 6주 배양 후 선발하는 것이었다. 이러한 선발은 유전자가 식물세포의 genomic DNA 안에 안정적으로 삽입되어 이루어졌음을 서던 블롯 분석 및 후대검정 등을 통하여 확인할 수 있었다. 이러한 장기적이고 대량의 배양이 가능한 벼 캘러스의 효율적인 선발 시스템은 벼를 이용한 유용 재조합 단백질의 산업화에 실속있는 기술로 기여할 수 있을 것이다.

  • https://doi.org/10.9787/KJBS.2017.49.3.170 인용

Characterization of panicle architecture and vascular bundle number at panicle neck on Oryza glaberrima introgression lines

  • Demeter, Zita;Inoue, Shota;Fujita, Daisuke
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.94-94
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    • 2017

  • Oryza sativa and Oryza glaberrima are rice cultivars distributed in Asia and Africa. There are several differences between these cultivars in morphological characteristics such as panicle structure and especially the secondary branch number of O. glaberrima is less than that of O. sativa. Generally, branch number of panicle related to a large vascular bundle number (VBN) among O. sativa and there is a wide variation of the VBN of the peduncle from where the bundles enter into the rachis branches. However, there is less information about VBN in O. glaberrima and also the relationship between VBN and branch numbers, the primary branch number (PBN) and secondary branch number (SBN). Additionally, the genetic factor for VBN and branch number in O. glaberrima is not completely exploited. In this study, phenotypic variation for VBN and panicle structure were investigated using a set of 40 $BC_3$ -derived from IRGC 104038 (O. glaberrima from Senegal) and 35 $BC_4$ -derived from IRGC103777 (O. glaberrima from Mali) introgression lines with a genetic background of japonica rice Taichung 65. Taichung 65 had 11.8 PBN, 16.0 SBN and 11.5 VBN, while IRGC 103777 had 12.0 PBN, 15.0 SBN and 15.3 VBN. The introgression lines derived from IRGC 104038 had range from 9.0 to 14.4 in the PBN, range from 9.6 to 33.5 in the SBN and range from 9.8 to 14.8 in the VBN. Additionally, the introgression lines derived from IRGC 103777 had range from 9.0 to 18.5 in the PBN, range from 10.3 to 39.0 in the SBN and range from 9.0 to 15.3 in the VBN. Among two set of introgression lines, there are significant correlation between VBN and PBN. Multiple introgression lines indicated higher PBN, SBN and VBN than Taichung 65 and these examined characteristics are supposedly controlled by quantitative traits loci. The genetic factor related to VBN and panicle architecture can be revealed using segregating population in future study.

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