• 동의생리병리학회지
• /
• 제20권6호
• /
• pp.1572-1575
• /
• 2006

Water extract from Cnidii Rhizoma (CRW) was tested for colon cancer chemopreventive activity by measuring the induction of phase II detoxification enzyme activity [quinone reductase (QR) and glutathione S-transferase (GST)] and glutathion (GSH) levels and ornithine decarboxylase (ODC) activity in cultured human colorectal adenocarcinoma HT-29 cells. CRW inhibited cell proliferation in cultured HT-29 cells. CRW induced QR activity in a dose-dependent manner in a concentration range of 0.1${\sim}$5.0 $mg/m{\ell}$. GST activity was also induced with the treatment of CRW in HT-29 cells. In addition GSH levels was increased with CRW. CRW inhibited ODC activity, a key enzyme of polyamine biosynthesis, which is enhanced in tumor promotion. These results suggest that CRW has colon cancer chemopreventive activity by increasing phase II enzyme activity and GSH levels and inhibiting ODC activity in vitro.

• Asian-Australasian Journal of Animal Sciences
• /
• 제7권1호
• /
• pp.119-124
• /
• 1994

Experiments were performed to determine age-associated changes in ornithine decarboxylase (ODC) gene and Ha-ras cellular oncogene expression in tissues of female rats. In the kidney, ODC mRNA levels did not show age-associated changes, while ODC enzyme activities were decreased with advancing age from 3 to 10 months. These results suggest that post-transcriptional mechanism (s) are involved in the age-dependent decrease in renal ODC enzyme activity. In addition, we found no correlation between testosterone-induced renal ODC expression and DNA methylation pattern. Ha-ras mRNA levels in brain decreased as animals aged from 3 to 6 months, while renal Ha-ras mRNA levels were not influenced by age. Results demonstrate the age-dependent expression of Ha-ras in a tissue-specific manner.

• Journal of Genetic Medicine
• /
• 제16권2호
• /
• pp.85-89
• /
• 2019

Hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome is a rare autosomal recessive urea cycle disorder. HHH is caused by a deficiency of the mitochondrial ornithine transporter protein, which is encoded by the solute carrier family 25, member 15 (SLC25A15) gene. Recently, government supported Korean newborn screening has been expanded to include a tandem mass spectrometry (MS/MS) measurement of ornithine level. We report a case of a neonate with HHH syndrome showing a normal MS/MS measurement of ornithine level. A female newborn was admitted to neonatal intensive unit due to familial history of HHH syndrome. Her parents were consanguineous Parkistani couple. The subject's older sister was diagnosed with HHH syndrome at age of 30 months based on altered mental status and liver dysfunction. Even though the subject displayed normal ammonia and ornithine levels based on MS/MS analysis, a molecular test confirmed the diagnosis of HHH syndrome. At 1 month of age, amino acid analysis of blood and urine showed high levels of ornithine and homocitrulline. After 11 months of follow up, she showed normal growth and development, whereas affected sister showed progressive cognitive impairment despite no further hyperammonemia after protein restriction and standard therapy. Our report is in agreement with a previous Canadian study, which showed that neonatal samples from HHH syndrome patients demonstrate normal ornithine levels despite having known mutations. Considering the delayed rise of ornithine in affected patients, genetic testing, and repetitive metabolic testing is needed to prevent patient loss in high risk patients.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권16호
• /
• pp.7031-7038
• /
• 2015

Background: Arginine may play important roles in tumor progression by providing ornithine for polyamine biosynthesis, required for cell growth. The aim of this work was to determine the expression of arginine metabolic pathway enzymes in head and neck squamous cell carcinoma (HNSCC) in northeast India. Materials and Methods: The expressions of arginase isoforms (ARG1 and ARG2), ornithine aminotransferase (OAT) and ornithine decarboxylase (ODC) were examined in fifty paired HNSCC and adjacent non-tumor tissues by immunohistochemistry. Immunocytochemistry, semiquantitative reverse transcription sq-PCR and quantitative real-time qPCR were used to assess protein and mRNA expressions in peripheral blood of fifty HNSCC patients and hundred controls. Results: ARG1 and ODC protein and mRNA were strongly expressed in peripheral blood from HNSCC patients. No ARG2 expression was observed. In vivo, expression of ARG1, ARG2 and ODC was significantly higher in tumor than in non-tumor tissues. Most tumors expressed low levels of OAT, with no difference in tissues or blood, compared to controls. The absolute extent of maximal ARG1 upregulation with qPCR showed 6.23 fold increase in HNSCC. Conclusions: These findings strongly suggest that in HNSCCs, the ARG1 pathway is stimulated leading to the formation of polyamines as indicated by higher ODC expression, which promote tumor growth.

• Molecules and Cells
• /
• 제28권6호
• /
• pp.575-581
• /
• 2009

Ornithine decarboxylase (ODC) is a rate-limiting enzyme in the biosynthesis of polyamines, which are essential for cell growth, differentiation, and proliferation. This report presents the characterization of an ODC-encoding cDNA (SlitODC) isolated from a moth species, the tobacco cutworm, Spodoptera litura (Lepidoptera); its expression in a polyamine-deficient strain of yeast, S. cerevisiae; and the recovery in polyamine levels and proliferation rate with the introduction of the insect enzyme. SlitODC encodes 448 amino acid residues, 4 amino acids longer than B. mori ODC that has 71% identity, and has a longer C-terminus, consistent with B. mori ODC, than the reported dipteran enzymes. The null mutant yeast strain in the ODC gene, SPE1, showed remarkably depleted polyamine levels; in putrescine, spermidine, and spermine, the levels were > 7, > 1, and > 4%, respectively, of the levels in the wild-type strain. This consequently caused a significant arrest in cell proliferation of > 4% of the wild-type strain in polyamine-free media. The transformed strain, with the substituted SlitODC for the deleted endogenous ODC, grew and proliferated rapidly at even a higher rate than the wild-type strain. Furthermore, its polyamine content was significantly higher than even that in the wild-type strain as well as the spe1-null mutant, particularly with a very continuously enhanced putrescine level, reflecting no inhibition mechanism operating in the putrescine synthesis step by any corresponding insect ODC antizymes to SlitODC in this yeast system.

• BMB Reports
• /
• 제32권2호
• /
• pp.173-180
• /
• 1999

The effects of agmatine on the enzymes responsible for the biosynthesis of polyamines, the resultant levels of polyamines, and their effect on the growth of DU145 human prostate tumor cells were investigated. When agmatine was added to the medium, ornithine decarboxylase (ODC, EC 4.1.1.17) activity was substantially reduced, but S-adenosylmethionine decarboxylase (SAMDC, EC 4.1.1.50) activity increased markedly. These changes in ODC and SAMDC activities were the result of an induction of ODC-antizyme and a decreased turnover rate of SAMDC in the presence of agmatine. Accordingly, there was a decrease in the intracellular levels of putrescine and spermidine but an increase in the intracellular level of spermine. Cell growth was markedly inhibited by agmatine treatment and this inhibition was not recovered by the addition of putrescine or spermidine. Our results suggest that agmatine alters the intracellular amounts of polyamine in the cells, closely related to the inhibition of cell growth.

• 한국식품영양학회지
• /
• 제26권4호
• /
• pp.945-951
• /
• 2013

배추김치 제조 후 익힘 시간을 달리한 후 김치냉장고에 저장한 김치의 이화학적 특성과 오르니틴 함량 변화를 조사하기 위하여 익힘 및 저장 기간별 pH, 산도, 유산균 수 변화, 유산균 속 분포 및 오르니틴 함량 변화 패턴을 조사하였다. 배추김치는 조제 후 $15^{\circ}C$에서 각 시간 별로 익힘 과정을 거친 후 -$1.4^{\circ}C$의 김치냉장고에 60일까지 보관하였다. 본 실험에 사용한 시료는 익힘시간에 따라서 32 hr(S1), 36 hr(S2), 40 hr(S3), 44 hr(S4), 48 hr(S5)라 명명하였다. 김치냉장고 보관 모드로 전환하기 전 $15^{\circ}C$에서의 저장 시간을 상대적으로 길게 준 S5구에서 S1, S2구에 비하여 pH 감소가 좀 더 큰 것으로 나타났으며, 보관 시간이 지남에 따라 pH가 4.3~4.4까지 낮아지는 것을 알 수 있었다. 젖산농도의 경우 $15^{\circ}C$에서의 저장 시간을 상대적으로 길게 준 S5구에서 젖산농도 증가가 좀 더 큰 것으로 나타났으며, 보관 시간이 지남에 따라 젖산농도는 0.70~0.74까지 증가하는 것을 알 수 있었다. 유산균의 경우 모든 시료가 저장 초기 20일 경과까지 수가 급격히 증가하였고, 20일 이후에는 S4, S5 시료가 S1, S2, S3 시료에 비하여 좀 더 증대되는 경향을 보였다. 유산균 속은 저장 40일 기준으로 S5 시료에서 바이셀라 속 균이 50% 이상 검출되었다. 오르니틴 함량은 모든 시료에서 저장 40~50일차에 최대치를 보였으며, S4, S5 시료의 경우 오르니틴 함량은 100그램당 최대 130mg과 170mg까지 증대되었다. 그러나 S1, S2, S3 시료의 경우는 오르니틴 함량 증대 폭이 상대적으로 적었다. 이들 결과로부터 김치냉장고 조건에서 김치를 저장하기 전 $15^{\circ}C$ 조건에서 44~48시간을 발효한 후 김치냉장고에 40~50일 보관하면 김치 100그램 중의 오르니틴 함량을 최대 130~170mg까지 증대시킬 수 있을 것으로 판단되며, 그 중에서도 S5 (48 hr) 조건이 가장 우수한 것으로 조사되었다.

• Journal of Acupuncture Research
• /
• 제17권1호
• /
• pp.129-142
• /
• 2000

Gamdutang aqua-acupuncture solution(GAS), Gamdutang water-extracted solution(GWS) and Degamdutang aqua-acupuncture solution(DGAS) were prepared and tested for potential antitumor activities. It was used three biomarkers (quinone reductase, omithine decarboxylase, glutathione) to test chemopreventive potentials of GAS, GWS, DGAS. GAS was potent inducer of quinone reductase activity in Hepalclc7 murine hepatoma cells in culture, whereas GWS is less potent. GAS, GWS and DGAS were significantly induced quinone reductase activity in cultured rat normal liver cell, Ac2F. Glutathione levels were increased about 1.8-fold with GAS, 1.0-1.1 fold with GWS, DGAS in cultured murine hepatoma hepaiclc7 cells. In addition glutathione s-transferase levels were increased with GAS, GWS and DGAS. The effects of GAS, GWS and DGAS were tested on the growth of Acanthamoeba castellanii. Proliferation of Acanthamoeba castellanii was inhibited by GAS, GWS and DGAS at concentradons of $1{\times}$ and $5{\times}$. These results suggest that GAS has chemopreventive potential by inducing quinone reductase and quinone reductase activities, inhibition of ornithine decarboxylase activity, and increasing glutathione levels.

• Journal of Microbiology and Biotechnology
• /
• 제17권9호
• /
• pp.1546-1549
• /
• 2007

Chitosan oligosaccharide (COS, 3 kDa

• 생약학회지
• /
• 제30권3호
• /
• pp.261-268
• /
• 1999

Lonicerae flos aqua-acupuncture solution (LFAS) and Lonicerae flos water-extracted solution (LFWS) were prepared and tested for chemopreventive potentials. Three biomarkers [quinone reductase (QR), ornithine decarboxylase (ODC), glutathione(GSH)] were used to test chemopreventive potential of LFAS. LFAS was potent inducer of QR activity in Hepa1c1c7 murine hepatoma cells, whereas LFWS is less potent. LFAS and LFWS were also induced QR activities in cultured human hepatoma Hep3B cells. The effect of LFAS and LFWS were tested on the growth of Acanthamoeba castellanii. Proliferation of Acanthamoeba castellanii was inhibited by LFAS and LFWS at concentrations of $0.1{\times},\;0.5{\times}\;1{\times},\;and\;3{\times}.$ In addition, GSH levels were increased about 2-fold with LFAS and 1.5-fold with LFWS in cultured murine hepa1c1c7 cells. LFAS and LFWS were also shown to increase GSH levels in human Hep3B cells. These results suggest that LFAS has chemopreventive potential by inducing QR activity, inhibition of ODC activity and increasing GSH levels.