• Title/Summary/Keyword: ornithine decarboxylase

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Antimicrobial resistance and pulsed-field gel electrophoresis (PFGE) patterns of Salmonella Gallinarum isolated from chicken (닭에서 분리한 Salmonella Gallinarum의 약제내성 및 PFGE 양상)

  • Bae, Jong-Chul;Kim, Seong-Guk;Kim, Young-Hoan;Jo, Min-Hee;Lee, Young-Ju;Park, Cheong-Kyu
    • Korean Journal of Veterinary Service
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    • v.32 no.2
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    • pp.155-163
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    • 2009
  • Fowl typhoid (FT) is a septicemic disease caused by Salmonella Gallinarum. The purpose of this study was to investigate the antimicrobial resistance and pulsed-field gel electrophoresis (PFGE) patterns of S. Gallinarum isolated from chicken. During 1999 to 2004, there was isolated a total of 100 strains in liver and spleen. The biochemical characteristics of S. Gallinarum isolates was nonmotile, no production of H$_2$S, glucose gas, non-fermented rhamnose, indole-negative, fermentation of dulcitol, mannitol, maltose, and ornithine decarboxylase. At antimicrobial susceptibility, all of isolates were susceptible to amoxicillin/clavulanic acid, amikacin, neomycin, kanamycin, norfloxacin and enrofloxacin. One hundred isolates were divided into 54 resistant patterns and 37 strains was 6-multi drug resistance. PFGE of Xba I restriction fragments of S. Gallinarum isolates was 20 patterns.

Biochemical Characteristics and Antibiotic Resistant Patterns of Klebsiella pneumoniae (Klebsiella pneumoniae 분리균의 생화학적 특성과 항생물질 내성유형 연구)

  • Lee, Hun-Ku;Kim, Ki-Sang;Lee, Bok-Kwon;Chung, Tae-Hwa;Lee, Hyung-Hoan
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.4
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    • pp.427-433
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    • 1987
  • One hundred and thirty-six strains of Klebsiella pneumoniae were isolated from clinical specimen taken from pediatric patiants at 6 different hospitals and identified, characterized and investigated on the patterns of antibiotic resistance. The 136 strains showed the positive reactions in the 17 tests; Voges-Proskauer, ONPG, cirate utilization, KCN degradation, productions of lysine decarboxylase, acid and gas from glucose, utilizations of malonate, manitol, rhamnose, salicin, sucrose, raffinose, arabinose, lactose, sucrose, inositol and raffinose, but the strains showed the negative reactions in the 8 tests; production of $H_2S$, indole, arginine dehydrolase, ornithine decaraoxylase, phenylalanine deaminase, motility, methly red and gelatin liquefaction. 41 did not utilize dulcitol, and 32 did not utilize adonitol. The 36 out of them produced klebecin. The 136 strains were resistant to ampicillin, 2 to gentamicin, 14 to cephalothin, 16 to chloramphenicol, 8 to kanamycin, 13 to streptomycin, and 17 to tetracycline. Twenty strains were resistant to 2 kinds of antibiotics 5 strains to 3, 4 to 4 and 1 to 6 and 7.

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Isolation and Antimicrobial Susceptibility Testing of Edwardsiella tarda from Channa argus in Korea (가물치(Channa argus)에서 분리된 Edwardsiella tarda 의 생화학 및 항생물질 내성 유형에 관한 연구)

  • Lee, Hun-Ku
    • Journal of fish pathology
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    • v.1 no.2
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    • pp.95-101
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    • 1988
  • During the period from August through October, 1988, 50 isolates of Edwardsiella tarda were isolated from 6 diseased cultured Channa argus in Dunchi island and Myung-ghi, near Pusan in Korea were examined by studying their biochemical and antibiotical reactions. The ill animals moved slowly and irregular-formed swimming at the surface of the corner. The symthoms were necrosis with hemorrhage on the body surface, head, gill region, and mouth. Some fish were observed dropsy of the belly. The bacteria grew slowly on Double Salmonella-Shigella agar, 24h, at $37^{\circ}C$ to form relatively small size (2mm diameter), smoothed and convexed form with transient or black in center of the colonis. They gave negative reactions to Voges-Proskauer, Simmon's citrate, urea, KCN (in growth), gelatin, arginine dehydrolase, phenylalanine deaminase and many sugars. The isolates showed positive reactions to $H_2S$ (in KIA agar), indol, Methyl-Red, motility, lysine and ornithine decarboxylase, and gas from glucose. 8 drugs tested as chloramphenicol, colistin, gentamicin, kanamycin, lincomycin, nalidixic acid spectinomycin, and tetracycline. All cultures were resistant to colistin, lincomycin and spectinomycin respectibly, but sensitive to kanamycin and nalidixic acid. Three strains showed resistance to chloramphenicol and 2 isolates among them were resistant to two drugs(gentamicin and tetracycline), coincidentally.

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Pattern of antimicrobial resistance and biochemical characteristics of Salmonella Typhimurium isolated from diseased pigs in Gyeongbuk province (경북지방 환돈에서 분리한 Salmonella Typhimurium의 생화학적 성상 및 약제내성 패턴)

  • Kim, Seong-Guk;Eom, Hyun-Jung;Kim, Soon-Tae;Jang, Young-Sul;Jo, Min-Hee
    • Korean Journal of Veterinary Service
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    • v.33 no.1
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    • pp.51-57
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    • 2010
  • Salmonella Typhimurium is a virulent pathogen for human and animal. We studied serotypes, biochemical characteristics, and antimicrobial resistance of S. Typhimurium isolated from diseased pigs in Gyeongbuk province over 1998 to 2008. One hundred sixteen isolates were identified as S. Typhimurium by biochemical characteristics and serotypes from 90 farms. The biochemical characteristics of S. Typhimurium isolates was production of $H_2S$, indole-negative, fermentation of mannitol, dulcitol, sorbitol, inositol, rhamnose, and maltose, and ornithine decarboxylase. At antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, cefepime, ciprofloxacin, while were highly resistant streptomycin, cephalothin, enrofloxacin, nalidixic acid, apramycin, chloramphenicol, tetracycline. The isolates were divided into 65 resistant patterns and 47 of the isolates were shown as a DT104 ASSSuT resistant phenotype.

Naturally-Occurring Novel Anticatcinogens : Conjugated Dienoic Derivatives of Linoliec Acid (CLA) (새로이 분류된 천연 항암제 : Conjugated Dienoic Derivatives of Linoleic Acid (CLA))

  • 하영래;마이클파리자
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.4
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    • pp.401-407
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    • 1991
  • Anticarcinogenic conjugated dienoic derivatives of linoleic acid (CLA) is present in grilled beef, cheese, and related foods, CLA is generated via isomerization of linoleic acid in the cow's rumen by anaerobic bacteria and food proceessing as well. Another source of CLA is its endogenous generation via the carbon centered free radical oxdation of linoleic acid. We propose that the formation and generation of CLA in vivo represents a previously unrecognized in situ "defense mechanism" against membrane attack by oxygen free radicals. The cis, 9-trans, 11 CLS isomer is selectively incorporated into cellular phospholipid, which exhibits a potent antioxidant, reduces the activation of 2-amino-3-methylimidazo, [4,5-f] quinoline (IQ) for baxterial mutagenesis, and inhibits ornithine decarboxylase(ODC) activity induced by 12-0-tetradecanoylphorbol-13-acetate (TPA). We believe that at least these biological activities of CLA explain the anticarcinogenic activity of CLA.

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A STUDY ON SPECIFIC ACTIVITIES OF ENZYMES IN 7, 12-DIMETHYLBENZ(A)ANTHRACENE(DMBA)-INDUCED RAT SUBMAXILLARY GLAND CARCINOGENESIS (백서의 7, 12-Dimethylbenz(a)anthracene 유도 악하선 종양발암과정에서의 효소 특이활성도에 대한 연구)

  • Shim, Hyun-Goo
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.12 no.1
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    • pp.27-40
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    • 1990
  • In recent years, tissue antigens and enzymes that will serve as phenotypic markers for malignant cells are becoming increasingly important as diagnostic aids. This study was undertaken to investigate the specific activities of these enzymes in DMBA-induced rat submaxillary gland carcinogenesis. One hundred and twenty Sprague-Dawley rats about 100 gms of body weight were used. In experimental group, DMBA pellet (5mg) was implanted into right submaxillary gland and sham operation was performed into left gland to serve as control. The animals were sacrificed every three weeks up to 15 weeks. Submaxillary glands were excised on both sides and enzyme assays for ${\gamma}-glutamyl$ transpeptidase (GGT), 5'-Nucleotidase, Ornithine decarboxylase(ODC) and Acetyl-Co A carboxylase were carried out biochemically. The obtained results were as follows ; 1. In control group, there was no significant weight change of submaxillary gland, while experimental group, weight was increased remarkably about 7-fold at 15th week since DMBA implantation. 2. In control group, there was no change in specific activities of enzymes during the experimental period. 3. GGT activity was rapidly increased reaching a peak of 1.766${\pm}$0.082units/mg of DNA, 8-fold greater than that of onset. 4. 5'-Nucleotidase activity was increased reaching a peak of $362.1{\pm}53.2{\mu}moles/mg$ of DNA at 9th week. 5. ODC activity was rapidly increased, reaching a peak of 26.2${\pm}$4.8nmoles/mg of DNA at 9th week and quickly returned to that of control at 15th week. 6. Acetyl-Co A carboxylase activity was rapidly increased earlier than other enzymes, reaching a peak of 0.178${\pm}$0.013units/mg of DNA at 6th week and quickly declined to the control level at 15th week.

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A Study on Effect of Electric Field for Carcinogenesis of Strontium 90 (Strontium 90의 골수 발암성에 대한 전계장의 영향에 관한 실험적 연구)

  • 정문호;두옥주
    • Journal of Environmental Health Sciences
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    • v.20 no.3
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    • pp.61-77
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    • 1994
  • Sprague Dawley rats were exposed to electric fields (6,000 V, 10 kV/m, 30 min/day, 6 days per week) and injected strontium 90 (681 kbq/rat, one time) through abdominal cavity (strontium 90 and electric field complexed exposure group). In parallel, series with the electric field exposure only, strontium 90 injection only and control groups were run. Every group was consisted of 110 rats (55 male and 55 female). This animal experiment was performed from May to December in 1993. This results were conducted to investigate the effect of electric field for 11 weeks. The results are summarized as follows: 1. Ornithine decarboxylase (ODC) activity in rat's bone marrow cells: The ODC values was significantly increased in Sr$^{90}$ injection group and Sr$^{90}$ and electric field complexed exposure group as compared with that of control group (p<0.05). The ODC value was significantly decreased in electric field and Sr$^{90}$ complexed exposure group in comparison with Sr$^{90}$ injection group (p<0.05). The ODC values of electric field only exposure group was not different to that of control group (p>0.05). 2. The amount of Sr$^{90}$ accumulation in the femur, kidney and spleen:The accumulation amount of Sr$^{90}$ in the femur of Sr$^{90}$ injection group represented higher value than that of electric field and Sr$^{90}$ complexed exposure group (p<0.05). In the kidney and spleen, the difference between electric field and Sr$^{90}$ complexed exposure group and Sr$^{90}$ injection group wasn't observed. 3. The counts of white cells in blood of Sr9?injection group was decreased as compared with the value from control group and electric field and Sr$^{90}$ complexed exposure group (p<0.05). The rat's body weight, red blood cell counts and the weight data of liver, kidney and spleen did not show differences among four groups.

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A Study on Characteristics of Escherichia coli Isolated from Fish in Market (시판 어패류에서 분리한 대장균의 특성에 관한 연구)

  • 김정현;이용욱;이후장;나승식
    • Journal of Food Hygiene and Safety
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    • v.12 no.4
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    • pp.354-360
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    • 1997
  • This study was intended that the biochemical patterns, bioserological characteristics, resistance of antibiotics, and transferable resistance patterns of 35 Dscherichia coli strains from 79 fish and shellfish samples in marine markets from August to October, 1995. The Standard plate count, coliforms and fecal coliforms were also counted in the 79 cases and analysed the correlationship each other. Geometric means of Standard plate count in seawater fish, shellfish, mollusca and crustacean were 1.4$\times$105 CFU/g, 4.0$\times$105 CFU/g, 2.4$\times$105 CFU/g, 4.7$\times$105 CFU/g, and those of coliforms were 1.3$\times$103 CFU/g, 4.8$\times$103 CFU/g, 8.9$\times$102 CFU/g, 5.8$\times$103 CFU/g. There were no fecal coliforms in the fish and mollusc. However, the geometric means of coliforms in the shellfish and crustacean (1.1$\times$101 CFU/100g, 10 CFU/100 g) were less than those of fish and mollusca. The important biochemical characteristics of E. coli distinguished from the shellfish and crustacean were motility, ornithine decarboxylase, mucate, esculin. The fermentative properties of E. coli were also sucrose, salicin, sorbitol, and raffinose. Of 35 isolates of E. coli, 13 strains (37.1%) showed the pathogenic O antisera, which were O:27 3 strains (23.1%), ):159 2 strains (15.4%) and ):148, O:119, O:142, O:158, O:136, O:18, O:128, and O:168 1 strain (7.7%),respectively.

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The antimicrobial susceptibility and plasmid profile of E coli isolates from wild bird (야생조류 유래 대장균의 항생제 감수성 및 plasmid profile)

  • Kwak Hyun-Jeong;Lee Woo-Won;Kim Jam-Hwan;Chung Kyung-Tae;Woo Byung-Gil;Lee Gang-Rok;Lee Dong-Soo
    • Korean Journal of Veterinary Service
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    • v.29 no.1
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    • pp.37-46
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    • 2006
  • The present study was conducted to investigate serologic characteristics, antimicrobial resistance and plasmid profile of 79 E. coli isolated from faecal sample of the wild bird in Busan province (Gangseo, Saha) during the period from November 2004 to February 2005. Many strains were positive for rafiinose (63.3%), sorbitol (96.2%), sucrose (73.4%) and few strains were positive for esculin, arginine dihydrolase, ornithine decarboxylase. Among 79 isolates, O serotypes were O55, O158, O18 and 47 strains (59.5%) were resistant to antimicrobial drugs such as tetracycline (40.5%), carbenicillin (27.8%). 15 strains showed 14 different plasmid bands composed of 1 to 6 bands. They were ranged from 1.6 to 43 kb and harbored 43 kb (11.4%), 5.3 kb (6.3%) and 11.4 kb (5.1%). Plasmid profile of 15 strains were 43 profile (2.5%), 2.4, 43 profile (2.5%) and 5.3, 11.4, 43 profile (2.5%).

Effect of Polyamine Biosynthesis InhibItion on the Microbicidai and Jumoriddal Activities in Mouse Macrophage (생쥐 대식세포의 감염균 치사활성과 종양 치사활성에 미치는 Polyamine 생합성 억제의 영향)

  • 이준백;정노팔
    • The Korean Journal of Zoology
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    • v.34 no.2
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    • pp.173-180
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    • 1991
  • The objective of this experiment was to examine the effect of polyamine depletion by polyamine biosynthesis inhibitors on microbicidal activity and tumoricidal activity in mouse mac-rophages. $\alpha$ -Difluoromethylomithine (DFMO), inhibitor of putrescine and spernidine biosynthesis, treatment in vivo for 6-8 days reduced chemiluminescence levels in thioglycollate-, lipo-polysaccharide (LPS), and BCG-treated mouse macrophages. An DFMO treatment in vitro inhibited production of tumor necrosis factor (TNF), in dose-dependent manner, and tumoricidal activity by macrophages. The effect of polyamine depletion by MO on ThF production and tumoricidal activity could be reversed by the addition of exogenous putrescine. These result indicated that the obserbed effect of DFMO on macrophage activities were mediated through inhibition of polyamines are, must be, required for optimal activities of macrophages.

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