• 대한약침학회지
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• 제21권2호
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• pp.48-60
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• 2018

Objective: The aim of this study is to optimize the extraction of beneficial substance from Cudrania tricuspidata leaves grown at Jirisan Mountain in South Korea by three different solvents depending on extraction time and at different temperature. Methods: The total phenolic contents were determined by the method reported by $S{\acute{a}}nchez$-Moreno et al. The total flavonoid contents were analyzed by Slinkard and Singleton. The DPPH radical scavenging activity was determined according to the method reported by Blois Results: The extraction yield for each solvent is 9.05-14.1%, 2.17-5.67%, and 2.3-3.9% for D.W., ethanol, and hexane, respectively. The overall results were maximized for the extract obtained with D.W. for 5 min at $100^{\circ}C$. The average phenol contents were 77.11, 45.64, and 0.343 mg/g at $100^{\circ}C$ in water, $78^{\circ}C$ in ethanol, and $68^{\circ}C$ in hexane, respectively. The flavonoid contents were the highest in the materials extracted with D.W., and were increased with increasing temperature, regardless of the extraction solvents, whether water (green), polar organic ethanol, or nonpolar organic hexane. In the ethanol extract, the flavonoid contents are increased gradually from 5.66 mg/g to 7.73 mg/g. The total flavonoid contents were proportional to the concentrations of the water extracts, ranging from 4.14 mg/g to 48.89 mg/g. The antioxidative activities of the water-extracted compounds are generally increased with increasing temperature from 42.5% to 85.5%. Those of the hexane extracts are increased slowly from 3.79% to 8.8%, while those of ethanol extracts are increased from 29.8% to 47.4%. Conclusion: The extraction yields were dependent upon solvents for extraction as well as extraction time and the temperature. The optimal extraction time was 5 min and the extraction yields were increased with increasing temperature excepted hexane. Of the three tested extraction solvents, the greenest solvent of water shows excellent results, suggesting that water is among the most effective solvents for natural sample extractions for general medicinal, pharmaceutical, and food applications.

• Environmental Engineering Research
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• 제19권2호
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• pp.123-130
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• 2014

Percolation is the important process of extracting the soluble constituents of a fine mesh, porous substance by passage of a liquid through it. In this study, bio-wastes were percolated under various conditions through continuous percolation processes, and the energy potential of percolate was evaluated. The representative bio-wastes from the K composting plant in Darmstadt, Germany were used as the sample for percolation. The central objective of this study was to determine the optimal amount of process water and the optimum duration of percolation through the bio-wastes. For economic reasons, the retention time of the percolation medium should be as long as necessary and as short as possible. For the percolation of the bio-wastes, the optimal percolation time was 2 hr and maximum percolation time was 4 hr. After 2 hr, more than two-thirds of the organic substances from the input material were percolated. In the first percolation process, the highest yields of organic substance were achieved. The best percolation of the bio-wastes was achieved when the process water of 2 L for the first percolation procedure and then the process water of 1.5 L for each further percolation procedure for a total 8 L for all five procedures were used on 1,000 g fresh bio-waste. The gas formation potentials of 0.83 and $0.96Nm^3/ton$ fresh matter (FM) were obtained based on the percolate from 1 hr percolation of 1,000 g bio-waste with the process water of 2 L according to the measurement of the gas formation in 21 days (GB21). This method can potentially contribute to reducing fossil fuel consumption and thus combating climate change.

• 한국식품위생안전성학회지
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• 제14권4호
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• pp.346-351
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• 1999

본 연구에서는 발효 식품의 제조에 유용한 항균성 물질을 생산하는 유산균 starter의 개발의 일환으로 장내 상존균으로 알려진 Lactobacillus acidophilus group유산균인 Lactobacillus amylovorus IMC-1 균주가 생산한 항균성 물질의 식품오염세균에 대한 항균 활성을 검토하였다. 내몽골 원산 치즈에서 분리된 L. amylovorus IMC-1 균주는 탈지유배지에서 37$^{\circ}C$에서 72시간 배양하였을 때 최대로 항균성 물질을 생산하였으며, 더 이상의 배양은 항균성 물질의 생산에 영향을 미치지 않았다. 겔 여과후의 항균성 물질은 식품오염세균인 Bacillus subtilis IFO 3025, staphylococcus aureus IAM 1011, Listeria monocytogenes VTU 206, Escherichia coli RB. 및 Pseudomonas fragi IFO 3458등과 같은 모든 피검균에 대하여 20units/ml 첨가로 항균활성을 나타내었다. 그리고 이 물질은 B. subtilis, E. coli및 Ps. fragi에 대해서는 살균 작용을 나타내었으며, Staphy. aureus와 L. monocytogenes에 대해서는 정균 작용을 보였다. 그 살균 작용은 용균 작용에 기인한 것임이 밝혀졌다. 또한 이 물질은 유기산, 과산화수소 및 단백질성 물질이 아님이 밝혀졌다.

• 한국유기농업학회지
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• 제21권4호
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• pp.569-588
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• 2013

The organic foods is the terminology calling the organic products, the organic livestock and the organic processing food. The value of organic food may be evaluated as the index of the nutrient content, the safety and the health promotion. The reason why consumers prefer the organic food in the market is because the value of health promotion to be obtained through its consumption is expected to be bigger than the expense according to the purchase of organic food. This study has the significance in developing the direct evaluation model like the human biomonitoring method. The hypothesis for this study is summarized, "If the metabolome of metabolic syndrome of decreases, the exposure of substance of health harm decreases, the number of hospital care decreases and the quality of life shall be improved if the organic food is incepted in the long term and the health care is managed well". The consumer's cooperative and the health consumer's cooperative select the experimental group of 100 persons and the comparative group of 100 persons in 5 areas in the whole country at the same time to verify this hypothesis. Its rate of change is compared and analyzed by measuring the blood and urine of each biomarkers such as the exposed agriculture pesticides, the nitrate in the body at intervals of 2 months for 1 year. Also, by letting participants in the experiment record the major activity such as the dietary intake and the exercise, etc., in the questionnaire and the performance evaluation form, the statistical analysis for the correlation of this with the metabolome, etc., is conducted. The time that is called minimum 1 year and a lot of expense are required to implement this model. Accordingly, the cooperative study by composing the consortium of the interdisciplinary and the interagency is desirable.

• 한국미생물·생명공학회지
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• 제24권6호
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• pp.664-670
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• 1996

The aim of the present research program was to develop a strain of actinomycetes producing antifungal substance. Soil samples were collected from various sites in Korea and a number of actinomycetes were isolated from the soil samples by applying selective agar for actinomycetes. Among over 440 isolates, a strain (NA-4803) producing antifungal substance against Trichophyton spp. Nannizzia otae and Pyricularia oryzae was selected. The strain NA-4803 was identified as strain similar to Streptoverticillium blastmyceticum with respect to morphological and physiological characteristics, lecithinase and lipolytic activity, degradation of organic compounds, resistance to antibiotics and utilization of carbon and nitrogen sources. But it showed some differences such as positive reaction of nitrate reduction, negative reaction of L-tyrosine degradation, resistance to cephaloridine, and utilization of I -rhamnose and inulin. The strain NA-4803 was named as Streptoverticillium sp. NA-4803.

• 대한의용생체공학회:의공학회지
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• 제38권5호
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• pp.242-247
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• 2017

This study presents a bio-chemical energy harvesting system which can generate electric power from bioorganic substance contained in vermicompost. It produced electricity by inoculating microbial fuel cell(MFC) with earthworm-composted food waste. The generated electricity was converted into usable voltage level for mobile electronics through power conditioning circuits. The implemented prototype showed $200{\mu}W$ of maximum output electric power, which successfully supplied a beacon device which continuously transmitted data to nearby smartphone without a battery. The proposed system can help develop portable or bio-mimetic energy supply for sustainable use with further improvement.

• KIEAE Journal
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• 제4권4호
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• pp.3-10
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• 2004

If move in in the new apartment usually, do that hundreds hazardous substance among air floats and is broken-down. In the case of the floor, organic compound turns in adhesives that is bitter when attach the reinforcement floor and material lumber and hazardous substance is exhausted much wallpaper and concrete. Therefore, to reveal maximum this research to apply on interior of building to live function of charcoal, at non combustible material two faces that can promise safety from fire, strong and satisfy light performance, and it is thing about development of sheathing that use recycling material for barren land of multi-function and surface treatment of charcoal board as well as can keep healthy indoor environment.

• 미생물학회지
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• 제23권4호
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• pp.241-247
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• 1985

Pseudomonas fluorescens에 의해 세포외에 생산된 furfural의 furoic acid로의 생물학적 전환을 촉진시키는 ninhydrin 반응에 양성인 대사물 (Ninhydrin positive Substance=NPS)을 ion exchange chromatography와 gel permeation chromatography 그리고 cellulose columm chromatography에 의하여 분리, 정제하였다. IR spectrophotometry와 $^H$-NMR spectrometry 그리고 $^{13}C-NMR$ spectrometry에 의해 이 NPS는 -$^H$ and $-NH_2$와 그리고 $-CH_2-OH$group을 갖는 유기물질로 추정된다.

• 한국환경복원기술학회지
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• 제8권3호
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• pp.13-20
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• 2005

This study was conducted to suggest the ecological restoration methods of the decomposed granite cut-slope by organic soil amendment materials. Field test carried out for the cut-slope with organic soil amendment materials method and other three revegetation methods in Cheongwon. Test revegetation plants were Festuca arundinacea, Lolium perenne, Dactylis glomerata, Lespedeza cyrtobotrya, and Arundinella hirta. The result of this study can be summarized as follows; 1. The soil hardness, the soil acidity, and the soil humidity of organic soil amendment materials method were at a suitable value for plants growth. And it was better as compared with other three revegetation methods of cutting-rock slopes. 2. The result of toxic substance investigation, all items were at a suitable for standard law. 3. During one year after seeding, most plants germinated and especially Festuca arundinacea and Dactylis glomerata grows well. Seedling numbers were 336.7 per $m^2$(after 6 months), 183.3 per $m^2$(after 10 months), and 353.3 per $m^2$(after 6 months). Ten months later after seeding, plants showed 80% ground coverage. Visual rate, plant height, and growth rate were excellence, Also, high plant growth in spring better than autumn.

• 한국물환경학회지
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• 제30권2호
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• pp.175-183
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• 2014

The influence of temperature on the treatment efficiency of chlorinated organic substances contained in groundwater by permeable reactive barrier which is composed of $Fe^{\circ}$ has been investigated by constructing the Pourbaix diagrams for Fe-$H_2O$ system at different temperatures based on thermodynamic estimation. In aerobic condition, the equilibrium potentials for $Fe^{\circ}/Fe^{2+}$ and $Fe^{2+}/Fe^{3+}$ were observed to increase, therefore, the dechlorination reaction for organic pollutants by $Fe^{\circ}$ was considered to decline with temperature due to the diminished oxidation of reactive barrier. The result for the variations of the ionization fraction of $Fe^{2+}$ and $Fe^{3+}$ ion in the pH range of 0 ~ 2.5 obtained by employing Visual MINTEQ program showed that the ionization fraction of $Fe^{2+}$ increased with pH, however, that of $Fe^{3+}$ decreased symmetrically and the extent of the variation of ionization fraction for both ions was raised as temperature rises. The equilibrium pH for $Fe^{3+}/Fe(OH)_3$ was examined to decrease with temperature so that the treatment efficiency of chlorinated organic substance was expected to decrease with temperature due to the enhanced formation of passivating film in aerobic condition. The change of the reactivity of a specific chemical species with temperature was defined quantitatively based on the area of its stable region in Pourbaix diagram and depending on this the reactivity of $Fe^{3+}$ was shown to decrease with temperature, however, that of $Fe(OH)_3$ was decreased monotonously as temperature is raised for $Fe^{3+}/Fe(OH)_3$ equilibrium system. In anaerobic condition, the equilibrium potential for $Fe^{\circ}/Fe^{2+}$ was observed to rise and the equilibrium pH for $Fe^{2+}/Fe(OH)_2$ were examined to decrease as temperature increases, therefore, similar to that for aerobic condition the efficiency of the dechlorination reaction for organic substances was considered to be diminished when temperature rises because of the reduced oxidation of $Fe^{\circ}$ and increased formation of $Fe(OH)_2$ passivating film.