• 한국유기농업학회지
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• 제19권spc호
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• pp.6-8
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• 2011

In order to produce the organic foods in accord with international standard, organic seeds should be used in organic farming. This study was conducted to establish the stable production of organic seed of waxy com by examining the growing characteristics, seed yield, and the economy for seed production by organic farming. The optimal sowing timing for organic seed production of waxy com hybrid was within 10 days of the $1^{st}$ of May with yield of 88~90% of conventional seed production. The optimal planting density was 41,600 plants/ha ($80{\times}30$ cm) for organic seed production of waxy com. The weight of 100 seeds and seed productivity increased at the planting ratio of 2:1 mother plant:male plant. Growth and seed production were improved by removing male plant at 7~10 days after silking. Organic fertilizer (mixed oil cake) was applied at a rate of 4~6 Mg/ha before sowing. Black plastic mulching was used for weed control. In addition, sex pheromone trap and bio-control agents were used for safe pest control and low labour cost.

• 한국초지조사료학회지
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• 제12권4호
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• pp.211-217
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• 1992

This experiment was conducted over a 3-year period (1990-1992) to evaluate the production and quality of orchardgrass (Potomac)-red clover (Kenland) mixture when orchardgrass + red clover mixture was sown at seed rates of 20+0. 18+2, 16+4, 14+6 and 12+8 kg/ha. Total herbage dry matter, crude protein and digestible organic matter production and organic matter intake were markedly affected by increasing rcd clover seed rate, those increased with increasing red clovcr seed rate due to the cnhancernent of red clover percentage of total herbagc in all 3-year. but significant differences occured between the orchardgrass alone and orchardgrass-red clover mixture with a seed rate of 4,6 and 8 kg/ha red clover (P<0.01). But on the other hand increasing red clover seed rate increased red clover percentage in total herbage, the mean red clover percentage of total herbage at the last cutting ranged from 54 to 65% in first year. 58 to 60% in second year, and 62 to 69% in third year, particularly it was observed that red clover was sown at seed rates of 6 and 8 kg/ha for red clover sown dominated strongly as the cutting times were progressed. Considering the maintenance of good botanical composition balance and the enhancement of production and quality of herbage, the optimized seed rate for orchardgrass-red clover mixtures, 16 kg/ha orchardgrass and 4 kg/ha red clover seems to be considered adequate.

• KSBB Journal
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• 제18권6호
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• pp.435-439
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• 2003

형질 전환된 담배 seed에서 담배 식물체를 유도하여 White 액체 배지에서 기관 배양하였다. 암조건, sucrose 2%의 조건에서 좋은 growth pattern을 나타내었고, 계대 배양은 외마디법을 이용하여 2주마다 하였다. 기관 배양에서 hGM-CSF production pattern을 보면, intracellular에서는 큰 변화 없이 약 30 ng/g의 일정한 농도를 나타내었다. Extracellular에서 hGM-CSF 농도는 배양 6일 이후부터 급속하게 증가하기 시작하여 배양 12일째에 약 0.2ng/$m\ell$의 농도를 나타낸다. 기관배양은 다른 식물세포 배양 시스템에 비해 생산되어진 단백질의 안정성이 크다는 장점에 비해 세포 내에서 배지 내로의 단백질 분비가 적다는 단점이 있다. 이를 극복하기 위해 다양한 permeabilizing agents를 투여하여 담배 세포의 permeability를 증가시키고자 하였다. 그 결과, Pluronic F-68과 PEG8000을 첨가한 경우 담배 세포에서 배지 내로의 단백질 분비가 원활해졌음을 확인할 수 있었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권2호
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• pp.156-156
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• 2001

The objective of this study was to investigate the effects of nutrients and culture conditions on morphology during the seed culture of C. acremonium ATCC 20339 Morphological factors such as hyphal length number of tips number of arthrospores were observed to investigate the relationship between seed morphology and CPC production. During the time course of seed culture, hyphal length was shortened and the number of arthrospores increased rapidly On the other hand the number of tips deceased rapidly and this was closely related to the hyphal length Mixed nitrogen sources of 3% solybean meal and 1% cotton seed flour were determined as the proper organic nitrogen sources, in terms of the morphological factors in the seed culture. This fact was proven in batch culture for the production of Cephalosporin C. It was also found that a proper agitation speed enhanced the morphological differentiation of C. acremonium ATCC 20339, thus improving the production of Cephalosporin C.

• Plant Resources
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• 제8권1호
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• pp.13-16
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• 2005

This study was conducted to develop effective production system in greenhouse for leaves and stems of Angelica acutiloba by fertilizing of Sta-Green in pots. The results obtained are summarized as follows. Germination rate of Angelica acutiloba seeds collected in 2003 was $13\%$, while germination rate of seeds Collected in 2004 was above $91\%$. Seed germination rate and plant biomass of Angelica acutiloba collected in 2004 were higher than seed gathering in 2003. Especially, plant growth and yield of Angelica acutiloba grown in pot(The pots was filled with soil mixtures of Sta-Green and Peat Moss mixed with 45:55 ratio.) was the highest. These results indicate that leaf and stem production of Angelica acutiloba can be improved by fertilizing of Sta-Green in pots and optimizing seed collecting time in greenhouse.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.357-365
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• 2019

Objective: The experiment was conducted to evaluate the effects of four fungal pretreatments on the nutritional value of Camellia seed residues, and to evaluate the feeding value of pretreated Camellia seed residues for ruminants. Methods: Camellia seed residues were firstly fermented by four lignin degrading fungi, namely, Phanerochaete chrysosporium (P. chrysosporium)-30942, Trichoderma koningiopsis (T. koningiopsis)-2660, Trichoderma aspellum (T. aspellum)-2527, or T. aspellum-2627, under solid-state fermentation (SSF) conditions at six different incubation times. The nutritional value of each fermented Camellia seed residues was then analyzed. The fermentation profiles, organic matter degradability and metabolizable energy of each pre-treated Camellia seed residue were further evaluated using an in vitro rumen fermentation system. Results: After 5 days of fermentation, P. chrysosporium-30942 had higher degradation of lignin (20.51%), consumed less hemicellulose (4.02%), and the SSF efficiency reached 83.43%. T. koningiopsis-2660 degraded more lignin (21.54%) and consumed less cellulose (20.94%) and hemicellulose (2.51%), the SSF efficiency reached 127.93%. The maximum SSF efficiency was 58.18% for T. aspellum-2527 and 47.61% for T. aspellum-2627, appeared at 30 and 15 days respectively. All the fungal pretreatments significantly improved the crude protein content (p<0.05). The Camellia seed residues pretreated for 5 days were found to possess significantly increased organic matter degradability, volatile fatty acid production and metabolizable energy (p<0.05) after the treatment of either P. chrysosporium-30942, T. koningiopsis-2660 or T. aspellum-2527. The fungal pretreatments did not significantly change the rumen fermentation pattern of Camellia seed residues, with an unchanged ratio of acetate to propionate. Conclusion: The fungi showed excellent potential for the solid-state bioconversion of Camellia seed residues into digestible ruminant energy feed, and their shorter lignin degradation characteristics could reduce loss of the other available carbohydrates during SSF.

• 한국초지조사료학회지
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• 제15권2호
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• pp.124-131
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• 1995

In order to find out optimum seed production date according to different defoliation and flooding period of Italian ryegrass, this studies with Tetrone were canied out on the experimental field of Honam Crop Experiment Station from 1986 to 1988. Treatments included cutting date of Nov. 20 and Apr. 10 and flooding period of 5-25 days. In soil after experiment, organic matter, phosphate and silicate content increased, but potassium content decreased 0.16% than that before experiment. Heading and maturing date of Nov. 20 cutting were same with those of non cutting, those of Apr. 10 cutting lates 6 days to heading date and 2 days to maturing date. Plant height and culm length of Nov. 20 cutting were sirniller to those of non cutting, those of Apr. 10 cutting were shorter and panicle length have no difference between non cutting and cutting. Lodging of cutting treatment was reduced than that of non cutting. Lodgin was increased as flooding period was long, also loding of all treatment occured at 30 days after heading. Two cutting times of Nov. 20 and Apr. 10 have the most fresh yield, while non cutting have the most dry matter yield. Optimun seed productin date was considered to suitable when 35 days after heading (Jun. 14), at this time, seed production was 1,640 to 2,640 kg/ha. Also if flooding j u r y have, seed production was good between 10 days and 15 days after flooding.

• 아시안잔디학회지
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• 제5권2호
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• pp.81-86
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• 1991

The sludge, a waste of brewery industries, was examined for potentials as a natural organic fertilizer (or soil conditioner) for lawn. Trial products were measured for changes of physical, chemical properties in laboratory and seed germination and seedling growth in green house were also tested. The results are as the following:1The sludge from distilled liquor brewery contained high quantity of organic matter which had proper physical and chemical properties for lawn fertilizer (natural organic fertilizer, soil conditioner, top-dressing mix) . It showed good characteristics in handling and capabilities to be developed as commercial products for golf courses. 2.Sludge from beer company needs proper treatment to improve physical properties for futher degradiation. It is because aggregation of the sludge particles prevented microbial activities and changing to soluble form. 3.Green carbon can be used as carbon source for organic fertilizer production using brewery sludge, but it should not contain wood extract which inhibit seed germination and seedling growth.

• 한국유기농업학회지
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• 제27권1호
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• pp.1-16
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• 2019

The questionnaire survey was conducted on 225 farmers in Gyeonggi-do, Jeollanam-do and Jeollabuk-do. A total of 189 (84%) farmers responded. 72% of the respondents were males, 50.3% were aged 60 or older, and 51.3% had less than 5 years of farming experience. 78.8% of the respondents are pesticide-free, and 44.4% of respondents have less than 0.5 ha of farming scale. 61.4% of the cultivated crops were vegetable crops. The order of seeds and seedlings to buy was tomato (23.3%), cucumber (12.2%) and pepper (10.6%). The cost of purchasing seeds ranged from a minimum of 100,000 won to a maximum of 5 million won. 78.3% of respondents answered that they well-knew or knew about organic seeds. 78.3% of respondents answered that they knew or knew about organic seeds. Of the positive effects of mandatory use of organic seeds, 41.3% of respondents said they would increase confidence in organic certification. However, 41% of respondents who opposed the mandatory use of organic seeds said that "The strengthening of regulations will make organic agriculture more difficult." When the use of organic seeds is mandatory, 43.4% of the respondents favor direct support for the purchase of organic seeds, which should be supported politically by the state. When organic seeds were supplied, the disease resistant seeds (53.4%) was the preferred characteristic of organic seeds. For the optimal price of organic seeds, 38.6% of respondents wanted the same price as the commercialized conventional seed. In this study, the questionnaire was conducted for three major organic farming regions, but many of the respondents were judged to have a legal position on the mandatory use of organic seeds. Therefore, the results of this study can be used as a basic data for reviewing the legislation on the organic seed production and distribution suitable for the situation of Korean organic farming.

• 한국응용곤충학회지
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• 제22권2호
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• pp.130-137
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• 1983

A wide variety of pathogens are known io be seedborne, carried either as infectious mycelium internally or as contaminants on the seed coat. When seed is infected with a pathogen, the seed nay be rendered nonviable or it may remain viable but produce weak seedling. In some cases, the Infected seedling nay not be severely weakened, but nay serve as a source of primary inoculum within a community of plants. A recent problem nay be the dissemination of seedborne pathogens occurring as a result of the massive movements of seed, as a part of the 'Green revolution' Disease of great danger to agriculture may be introduced with seed from other parts of world. Seed treatment with organic mercury compounds in liquid form had become popular since about 1955. Organic mercury compounds contributed considerably to the increase in production of many crops and vegetables. In 1975, however, the use of organic mercury compound was forbidden because of doubts regarding their residual mammalian toxicity in agricultural products. Benomyl-thiram mixture, thiophanate methyl-;hiram mixture and TCMB have now been registered as seed disinfectants for the use of rice blast, brown spot and Bakanae disease. Oxathiinsthiram mixture has been registered as seed disinfectant for barley and wheat loose smut and leaf stripe of barley. Agricultural techniques have made such rapid progress that the nursery methods changed from the use of paddy nursery to box nursery designed for machine-transplanting. The spread of rice transplanting machines has caused increase of seedborne diseases. Among seedborne diseases, Bakanae disease has remarkably increased and causes much damage recently. In order to counter this trend, seed disinfectants must also be diversified. First, effective non-selective disinfectants need to be developed, and second, appropriate control methods always need to be prepared in parallel with the development of new techniques for cultivation.