• 분석과학
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• 제22권2호
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• pp.119-127
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• 2009

폐기물공정시험법과 내분비계 장애물질 시험방법을 이용하여 사업장폐기물소각재 24건 및 생활 쓰레기소각재 20건에 대하여 잔류성 유기오염물질의 분포특성을 확인하였다. 12종류의 잔류성 유기오염 물질중에서 7종을 GC-MSD (SIM-mode)로 정량분석을 하였다. 총 44건의 시료 중 21 시료에서 헥사클로로벤젠이 검출되었으며, 나머지는 모두 불검출이었다. 헥사클로로벤젠은 사업장폐기물의 소각재에서는 0.132-8.138 ng/g, 비산재에서는 0.195-5.765 ng/g, 그리고 생활쓰레기 소각장의 소각재에서는 0.270-1.828 ng/g, 비산재에서는 0.154-50.643 ng/g 정도로 각각 검출되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.357-365
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• 2019

Objective: The experiment was conducted to evaluate the effects of four fungal pretreatments on the nutritional value of Camellia seed residues, and to evaluate the feeding value of pretreated Camellia seed residues for ruminants. Methods: Camellia seed residues were firstly fermented by four lignin degrading fungi, namely, Phanerochaete chrysosporium (P. chrysosporium)-30942, Trichoderma koningiopsis (T. koningiopsis)-2660, Trichoderma aspellum (T. aspellum)-2527, or T. aspellum-2627, under solid-state fermentation (SSF) conditions at six different incubation times. The nutritional value of each fermented Camellia seed residues was then analyzed. The fermentation profiles, organic matter degradability and metabolizable energy of each pre-treated Camellia seed residue were further evaluated using an in vitro rumen fermentation system. Results: After 5 days of fermentation, P. chrysosporium-30942 had higher degradation of lignin (20.51%), consumed less hemicellulose (4.02%), and the SSF efficiency reached 83.43%. T. koningiopsis-2660 degraded more lignin (21.54%) and consumed less cellulose (20.94%) and hemicellulose (2.51%), the SSF efficiency reached 127.93%. The maximum SSF efficiency was 58.18% for T. aspellum-2527 and 47.61% for T. aspellum-2627, appeared at 30 and 15 days respectively. All the fungal pretreatments significantly improved the crude protein content (p<0.05). The Camellia seed residues pretreated for 5 days were found to possess significantly increased organic matter degradability, volatile fatty acid production and metabolizable energy (p<0.05) after the treatment of either P. chrysosporium-30942, T. koningiopsis-2660 or T. aspellum-2527. The fungal pretreatments did not significantly change the rumen fermentation pattern of Camellia seed residues, with an unchanged ratio of acetate to propionate. Conclusion: The fungi showed excellent potential for the solid-state bioconversion of Camellia seed residues into digestible ruminant energy feed, and their shorter lignin degradation characteristics could reduce loss of the other available carbohydrates during SSF.

• Bulletin of the Korean Chemical Society
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• 제34권5호
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• pp.1473-1476
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• 2013

Solution-based deposition of $CuIn_xGa_{1-x}Se_2$ (CIGSe) thin films is well known non-vacuum process for the fabrication of CIGSe solar cells. However, due to the usage of organic chemicals in the preparation of CIG precursor solutions, the crystallization of the polycrystalline CIGSe and the performance of CIGSe thin film solar cells were significantly affected by the carbon residues from the organic chemicals. In this work, we have tried to eliminate the carbon residues in the CIG precursor thin films efficiently by using soft-annealing process. By adjusting soft-annealing temperature, it is possible to control the amount of carbon residues in CIG precursor thin films. The reduction of the carbon residues in CIG precursors by high temperature soft-annealing improves the grain size and morphology of polycrystalline CIGSe thin films, which are also closely related with the electrical properties of CIGSe thin film solar cells.

• Applied Biological Chemistry
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• 제18권2호
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• pp.61-64
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• 1975

담배연작지의 토양중 토양살충제로 처리한 Heptachlor의 잔류분을 GLC로 분석하였다. Heptachlor의 토양중 잔류분은 모두 0.010ppm에 미달하였다. 한편 담배 연작지에 재배된 무우, 배추의 Heptachlor잔류량은 0.01 ppm이하로, 이수준은 같은 해인 3971년도 전국에서 수집, 분석한 두 채소의 Heptachlor 잔류량과 대등하였다. 무우 및 배추로 흡수, 이행하는 Hheptachlor잔류량은 토양 중 등 살충치의 농도에 비례하지 않었다.

• 한국유기농업학회지
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• 제6권1호
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• pp.45-50
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• 1997

Cultivation of peach by organic farming and cooperative form of work was attempted. The compost was made from egg shells and bread residues(bakery residues), falling leaves, and weeds. The cooperative teams of work were farmers, students and members of citizens' movement. The responses of participants for composting, though it's laboriousness and dirtiness, were favorable with feeling of doing good for social services. 20 chickens were raised in the peach orchard as one of the mixed farming. The pH and Ca content of ripened compost were 7.7 and 1.57 %, respectively. Controlling Aphids(plant louse)by ladybug was observed vividly. The size and sugar concentration were comparable to standard values even though rainy season and the number of damaged fruits were less than half(40%).

• 유기물자원화
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• 제17권2호
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• pp.81-92
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• 2009

본 연구에서 가축별 도살과정에서 발생되는 동물성잔재물의 발생 및 처리경로를 조사하고, 재활용업체의 재활용실태를 조사하여 관리상에 기초자료로서 활용하고자 수행하였다. 현재 동물성 잔재물은 동물별 전문화되어진 업종으로서 도살하고 있으며, 도살과정에서 발생되어진 각종 물질을 분리해체하여 사료 및 부산물퇴비로 이용되고 있는 것으로 조사되었다. 도축, 도살 잔재물은 식용으로 이용할 수 있는 것은 우선 이용하고, 식용으로 이용할 수 없는 뿔, 발톱, 유지 등은 재활용 처리되는 것으로 확인되었다. 도계 잔재물은 대부분 단미사료나 원형이용, 부산물비료로, 수산물 잔재물은 단미사료 등으로 재활용 되는 것으로 조사되었다. 동물성 잔재물을 재활용하는 업체는 발생원이 가까이 있는 전라남 북도와 경상남 북도 등에 다수 위치하는 것으로 나타났으며, 재활용업체도 이러한 도살장 부근에 위치하고 있는 것으로 조사되었다. 대부분의 재활용 업체는 10~60 ton/day의 시설용량을 가지고 있어서 시설규모에서 영세한 것으로 나타났으며, 재활용 처리상에 어려운 점은 부패에 따른 악취 등이 발생되어 이에 대한 대책이 요구되는 것으로 조사되었다. 재활용 대상물질에 대한 수집운반은 비용절감 및 원료확보 차원에서 직접 수집 운반하는 비중이 높은 것으로 조사되었다.

• Journal of Microbiology and Biotechnology
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• 제28권9호
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• pp.1502-1510
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• 2018

Organic solvent-tolerant (OST) enzymes are widely applied in various industries for their activity and stability in organic solvents, for their higher substrate solubility, and for their greater stero-selectivity. However, the criteria for identifying OST enzymes largely remain undefined. In this study, we compared the amino acid composition of 19 OST esterases with that of 19 non OST esterases. OST esterases have increased the ratio of Ala and Arg residues and decreased the ratio of Asn, Ile, Tyr, Lys, and Phe residues. Based on our amino acid composition analysis, we cloned a carboxylesterase (EstSP2) from a psychrophilic bacterium, Sphingomonas glacialis PAMC 26605, and characterized its recombinant protein. EstSP2 is a substrate specific to p-nitrophenyl acetate and hydrolyzed aspirin, with optimal activity at $40^{\circ}C$; at $4^{\circ}C$, the activity is approximately 50% of its maximum. As expected, EstSP2 showed tolerance in up to 40% concentration of polar organic solvents, including dimethyl sulfoxide, methanol, and ethanol. The results of this study suggest that selecting OST esterases based on their amino acid composition could be a novel approach to identifying OST esterases produced from bacterial genomes.

• Applied Biological Chemistry
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• 제41권1호
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• pp.73-77
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• 1998

두유박의 단백질 소재화를 위하여 셀룰레이즈(cellulase), 단백질 분해효소, 코지(koji), 효모 등의 복합처리에 의한 가용화 방법이 고형분 및 단백질 회수율, 아미노산, 유기산 조성에 미치는 영향을 고찰하였다. 셀룰레이즈와 단백질 분해효소를 복합 사용하여 두유박을 가수분해하는 것이 높은 고형분 회수율(80.2%) 및 단백질회수율(83.5%)을 얻을 수 있었고, 코지와 효모로 처리할 경우 유기산의 함량을 증대시킬 수 있었으며 양조간장 특유의 맛과 향을 내는데 효과적이었다. 특히 셀룰레이즈로 처리한 가수분해물에서 유기산 함량이 높았다. 유리 아미노산 함량은 코지보다 단백질 분해효소로 분해한 경우에서 높았으며 셀룰레이즈 처리는 유리 아미노산 함량에 큰 영향을 미치지 않았다. 두유박 가수분해물에 대한 관능검사 결과 코지로 가수분해한 시료가 관능적으로 우수하였으나 전체적인 기호도는 대조구로 사용된 양조간장에 비해 낮았다.

• Journal of Pharmaceutical Investigation
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• 제39권4호
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• pp.263-267
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• 2009

The purpose of this study is to evaluate the efficacy of stipulated cleaning process, and the prohibition of cross-contamination and microbiological contamination, which inadequate cleaning in multi-production could occur, through cleaning validation of multi-purpose facility used to produce five biopharmaceutical products as sterile injection. After production of five biopharmaceutical products such as hGH, rhGCSF, rhEPO, rhFSH and rhIFN using vial filling machine, the cleaning validation such as residual analysis of active ingredients or human serum albumin, measurement of total organic carbon (TOC), residual analysis of detergent and microbiological contamination were carried out. In the case of rhGH and rhGCSF clean validations, drug residues were not detected. Furthermore, in the case of rhEPO, rhFSH and rhIFN clean validations, human serum albumin residues were not detected. At TOC (total organic carbon) analysis, all clean validations gave the TOC of about average 137.93%, not more than 150% of acceptance criteria. At sodium analysis for the checking of residues of cleaning agent, sodium residues were not detected. In sterility test, they showed no microbiological contamination of bacteria and fungi. Thus, this cleaning validation was determined as successful in protection of cross-contamination and induction of safety in multi-purpose facility.

• 한국문화재보존과학회:학술대회논문집
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• 한국문화재보존과학회 2003년도 제18회 발표논문집
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• pp.38-41
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• 2003