• Journal of Oral Medicine and Pain
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• 제9권1호
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• pp.127-138
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• 1984

The author had tried to identify the sex from single tooth by detecting F-body of Y-chromosome in the nucleus of the dental pulp cells of 70 persons aged from 4 to 61 years under a fluorescent miscroscope. The results were as follows : 1. In the cell nuclei of male and female dental pulp at refrigeratory, the rate of F-body appearancd ranged 42-86%(average 61.06%) in male, while it was 0-6%(average 1.86%) in female, indicationg that male could be distinctly differentiated from female by F-body. 2. With male and female dental pulp puterfide by leaving in at room temperature, the rate of F-body appearance ranged 35-58%(average 48.20%) in male, 1-3%(average1.70%) in female, indicating that it was possible to distinguish male and female by F-body. 3. Even in heat-treated male teeth at $100^{\circ}C$,10 mins, the rate of F-body appearace proved to be 32-56%(averaged 42.50%), also indicating the possibility of identifying male. 4. When detecting of F-body in process of time, the rate of F-body appearance did not show major charges. 5. It was reaffirmed that F-body detection method was a positive determination method of male.

• Clinical and Experimental Pediatrics
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• 제57권9호
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• pp.384-395
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• 2014

Febrile seizure (FS) is the most common seizure disorder of childhood, and occurs in an age-related manner. FS are classified into simple and complex. FS has a multifactorial inheritance, suggesting that both genetic and environmental factors are causative. Various animal models have elucidated the pathophysiological mechanisms of FS. Risk factors for a first FS are a family history of the disorder and a developmental delay. Risk factors for recurrent FS are a family history, age below 18 months at seizure onset, maximum temperature, and duration of fever. Risk factors for subsequent development of epilepsy are neurodevelopmental abnormality and complex FS. Clinicians evaluating children after a simple FS should concentrate on identifying the cause of the child's fever. Meningitis should be considered in the differential diagnosis for any febrile child. A simple FS does not usually require further evaluation such as ordering electroencephalography, neuroimaging, or other studies. Treatment is acute rescue therapy for prolonged FS. Antipyretics are not proven to reduce the recurrence risk for FS. Some evidence shows that both intermittent therapy with oral/rectal diazepam and continuous prophylaxis with oral phenobarbital or valproate are effective in reducing the risk of recurrence, but there is no evidence that these medications reduce the risk of subsequent epilepsy. Vaccine-induced FS is a rare event that does not lead to deleterious outcomes, but could affect patient and physician attitudes toward the safety of vaccination.

• 대한디지털의료영상학회논문지
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• 제5권1호
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• pp.138-149
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• 2002

During the period from may to June 2000, a questionnaire study was carried out on 65 male radiological technologist who had been engaged at VDT work, to find out correlation their symptoms to VDT syndrome factors, work environment and condition. The results were as follows : 1. The mean age was 41.0 years, the mean relay working time was 3.75 hours and the mean duration of using computer monitor was 5.9 years, the satisfactory of working was 44.6% the others 55.4%, among these, 3.1% had symptoms before using the VDT and the others had not. 2. The prevalence of symptoms was the highest on wholebody tired, followed by eye, neck, low back, in decreased order and the lowest was fatique of wrist. 3. There was not significant correlation of symptoms to age, but significant to working time, working duration. 4. There was not significant correlation of symptoms to satisfactory. 5. Working desk, monitor, illuminations, temperature, and noise were significantly related to symptoms. 6. There was significant relation of symptoms to CT, MRI workers.

• Journal of Pharmaceutical Investigation
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• 제33권2호
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• pp.91-97
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• 2003

During the preparation of decoction from the mixture of Coptidis Rhizoma and Scutellariae Radix, insoluble copreciptate was formed. The coprecipitated product (COP) was composed of berberine and baicalin which was the active ingredient of Coptidis Rhizoma and Scutellariae Radix, respectively. COP was slightly soluble in water and could not be well absorbed after oral administration. This poor bioavailibility might be associated with its poor aqueous solubility. With the purpose of increasing the solubility and bioavailibility of COP, hydrolysis of COP by ${\beta}-glucuronidase$ was carried out. Hydrolyzed products (HOP) of COP were identified and assayed for active ingredients. The partition coefficient study, in situ absorption test, and pharmacokinetic study after oral administration were also performed. COP was found to be consisted of berberine and baicalin with molecular ratio of 1 to 1. This compound was hydrolyzed to berberine and baicalin by ${\beta}-glucuronidase$. The rate of hydrolysis was higher at higher temperature up to $50^{\circ}C$ and higher concentration of ${\beta}-glucuronidase$ up to 2500 unit under our experimental conditions. Baicalein, which is more liphophilic than baicalin, showed greater absorption in small intestine than baicalin did. The plasma concentrations of berberine and baicalein after oral administration of HOP were significantly higer than those of COP. The possible mechanism of increased bioavailibility of berberine and baicalein could be the hydrolysis of COP by ${\beta}-glucuronidase$. On the basis of the above results, it might be said that HOP should be a suitable preparation for increasing the bioavailibility of Coptidis Rhizoma and Scutellariae Radix.

• Journal of Oral Medicine and Pain
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• 제21권2호
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• pp.351-367
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• 1996

In the field Of individual identification in forensic Science, if the body is charred, it is sometimes impossible to identify the morphologic changes and charred tissue such as blood, muscle and bone can not be identified by forensic microbiologic method such as DNA typing. So the author used the characteristics of teeth which is relatively firm compare to other organs and stable to external environment such as heat and also possess cells needed for the DNA typing. The author conducted the experiment on teeth to detect DNA related to individual identification regarding to temperature in which other charredorgans can not be detected. The experiment was done on 64 extracted third molars consisted of unheated ones, and heated teeth to $100^{\circ}C$, $150^{\circ}C$, $200^{\circ}C$ for 45 min, 90 min, and 120 min respectively and to $250^{\circ}C$ for 45 min. DNA was extracted from each tooth and amplified fragment length polymorphism procedure(AMP-FLPs) using polymerase chain reaction(PCR) was applied and observed for the matching DNA in HumTH01 and HumCD4 locus and the followings Are the results : 1. It was able to detect matching DNA in HumTH01 and HumCD4 locus in every teeth which no heating has been done. 2. It was able to detect matching DNA in HumTH01 and HumCD4 locus in every teeth heated to $100^{\circ}C$ for 45, 90 and 120 min. 3. It was able to detect matching DNA in HumTH01 and HumCD4 locus in teeth heated to $l00^{\circ}C$, $200^{\circ}C$ for 45, 90, 120 min. 4. It was impossible to detect matching DNA in HumTH01 and HumCD4 locus in teeth heated to $250^{\circ}C$. So, it is possible to extract DNA from teeth that otherwise can not be extracted from other organs in the charred body and it can be concluded that teeth are highly reliable and applicatable as forensic odontology for individual identification.

• Journal of Applied Biological Chemistry
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• 제63권1호
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• pp.61-67
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• 2020

식용 식물 35종의 생리활성 부위를 대상으로 70% 에탄올 추출물의 구강 세균(Streptococcus mutans, Lactobacillus casei, Staphylococcus epidermidis, Klebsiella pneumoniae)에 대한 항균 활성을 확인하였다. 35종의 식물추출물 중 항균 스펙트럼이 가장 넓고 최소저해농도가 낮은 히비스커스 꽃 추출물을 구강 세균에 대한 항균 소재로 최종 선발하였다. 히비스커스 추출물의 항균 활성은 산성 조건에서 안정하였고, 열 안정성이 우수하였다. 히비스커스 추출물의 총 폴리페놀 및 총 플라보노이드 함량은 130.5 및 25.7 mg/g으로 나타났고, 유리라디칼 소거활성과 환원력의 항산화 활성이 우수하였다.

• Journal of Pharmaceutical Investigation
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• 제40권2호
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• pp.85-90
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• 2010

Docetaxel-loaded liposomes were prepared by emulsion-solvent evaporation method, then coated with chitosan at room temperature and lyophilized. This system was designed in order to improve solubility and stability of docetaxel in the GI tract for oral drug delivery. The solubilizing effect of some frequently used solubilizers and/or liposome was determined. Among the results docetaxel-loaded liposomes prepared with 0.5% TPGS as a solubilizer showed 100-fold higher solubility than docetaxel. In a stability test, mean particle size of different liposome formulations was measured by a particle size analyzer in simulated gastric fluid (SGF) and in simulated intestinal fluid (SIF). The particle size of uncoated liposomes was significantly increased compared with that of chitosan-coated liposomes in SGF, however, there was no significant difference between coated and uncoated liposome in SIF. It is evident that chitosan-coated liposomes were more stable in GI conditions. The release characteristics of docetaxel-loaded liposomes were also investigated in three buffer solutions (pH 1.2, 4.0, 6.8). Docetaxel release did not occur in pH 1.2 for 4 hrs. However, in pH 4.0 and 6.8 conditions, docetaxel was gradually released over 24 hrs as a sustained release. It seems that aggregation and precipitation of particles by electrostatic interaction might protect docetaxel from being released. In Conclusion, the results from this study show that the chitosan-coated liposomes may be useful in enhancing solubility and GI stability of docetaxel.

• Restorative Dentistry and Endodontics
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• 제23권2호
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• pp.682-689
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• 1998

The purpose of this study was to evaluate the sealing ability of the Microseal$^{(R)}$, which was new obturation system made by Tycom company, U.S.A. Forty-five extracted single-rooted human teeth were resected at cemento-enamel junction and divided three groups. All canals were prepared using Profile system, and then each group was obturated by lateral condensation technique (group 1), vertical condensation technique (group 2) and Microseal$^{(R)}$ condensation technique (group 3) with root canal sealer. Teeth were immersed in resorcinol-formaldehyde resin for 5 days at $4^{\circ}C$ and the resin was allowed to polymerize completely for 4 days at room temperature. Teeth were resected horizontally at 1 mm (level I), 2 mm (level II), 3 mm (level III) from the anatomical root apex using low speed microtome and examined with Image analyzer (IBASR, Zeiss co., Germany.) at ${\times}25$ magnification. The gab between the canal wall and the filling material, which was filled with the resin, was measured at each of the three levels. Each ratio of leakage was expressed percentage by calculating the ratio of the area of the resin to the total area of the canal and was analyzed statistically (one-way ANOVA). The results were as follows; 1. The mean ratio of leakage (%) was 6.46% at group 1, 3.06% at group 2, 11.27% at group 3. 2. When evaluating the ratio of leakage at the three levels, there was level I> level II> level III in all groups. Especially, the difference between level I and level III was statistically significant (p<0.05). 3. When evaluating the ratio of leakage at the three groups, there was group 3> group 1> group 2 at all levels. Especially the difference between group 2 and group 3 was statistically significant (p<0.05).

• 대한치과보철학회지
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• 제41권6호
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• pp.762-771
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• 2003

Statement of problem. In dentistry, the minimally prepared inlay resin-bonded fixed partial denture (FPD) made of new ceromer / fiber-reinforced composite (FRC) was recently introduced. However, the appropriate dimensions for the long-term success and subsequent failure strength are still unknown. Purpose. The aim of this study was to investigate the most fracture-resistible thickness combination of the ceromer / FRC using a universal testing machine and an AE analyzer. Material and Methods. A metal jig considering the dimensions of premolars and molars was milled and 56-epoxy resin dies, which had a similar elastic modulus to that of dentin, were duplicated. According to manufacturer's instructions, the FRC beams with various thicknesses (2 to 4 mm) were constructed and veneered with the 1 or 2 mm-thick ceromers. The fabricated FPDs were luted with resin cement on the resin dies and stored at room temperature for 72 hours. AE (acoustic emission) sensors were attached to both ends, the specimens were subjected to a compressive load until fracture at a crosshead speed of 0.5 mm/min. The AE and failure loads were recorded and analyzed statistically. Results. The results showed that the failure strength of the ceromer/FRC inlay FPDs was affected by the total thickness of the connectors rather than the ceromer to FRC ratio or the depth of the pulpal wall. Fracture was initiated from the interface and propagated into the ceromer layer regardless of the change in the ceromer / FRC ratio. Conclusion. Within the limitations of this study, the failure loads showed significant differences only in the case of different connector thicknesses, and no significant differences were found between the same connector thickness groups. The application of AE analysis method in a fiber-reinforced inlay FPD can be used to evaluate the fracture behavior and to analyze the precise fracture point.

• 한국융합학회논문지
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• 제12권8호
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• pp.187-195
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• 2021

최근 치과에서 사용되는 10종의 폴리머 기구에 대한 세포독성을 평가하기 위하여 L-929 세포를 이용한 MTT 시험을 시행하였다. 검체를 4g 당 20mL의 비율로 제조한 37℃의 RPMI 1640 용액에서 24시간 동안 용출한 후 식품의약품안전처 고시 제2020-12호 의료기기 생물학적 안전에 관한 공통기준규격에 따라 검체 용출액과 공시험액, 음성 및 양성대조를 사용하여, 37℃, 5% CO₂ Incubator에서 24시간 배양하여 ELISA reader로 판정한 결과, Intraoral camera는 용출물 농도 약 50%에서 약 70% 이상의 세포 생존율을 나타냈나 Plastic impression tray, 3D printing tweezer, Impression disposable syringe, Dental floss holder, Hand implant scaler, Surgical retractor, Oral scanner tip, Dental mirror, Water pick tip은 모두 용출물 농도 100%에서 70% 이상의 세포 생존율로 세포독성을 나타내지 않아 구강점막에 직접 접촉하여 사용이 가능한 기구로 평가되었다.