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http://dx.doi.org/10.15207/JKCS.2021.12.8.187

Cytotoxicity(MTT) evaluation of dental instruments made of polymers  

Choi, Eun-Mi (Dept. of Dental Hygiene, Kyungdong University)
Publication Information
Journal of the Korea Convergence Society / v.12, no.8, 2021 , pp. 187-195 More about this Journal
Abstract
In order to assess the cell toxicity of 10 instruments made of polymers, the MTT assay which utilizes the L-929 cell was selected. Specimens were eluted at a temperature of 37℃ for 24 hours at a rate of 4g per 20mL, RPMI 1640, and then was positively and negatively contrasted with a control test solution, in accordance with the Notification No. 2020-12 Protocols of Medical Apparatus Biological Safety from the Ministry of Drug and Food Safety. As a result of 24 hours of incubation in 37℃, 5% CO2 Incubator and assessment using an ELISA reader, the results of Intraoral camera indiciated a cellular viability of more than 70% at a 50% eluate. But, the Plastic impression tray, 3D printing tweezer, Impression disposable syringe, Dental floss holder, Hand implant scaler, Surgical retractor, Oral scanner tip, Dental mirror, and the Water pick tip all reported a cellular viability of more than 70% at a 100% eluate, which indicates that do not exhibit cytotoxicity, thus allowing it to be used in contact with the mucous membrane of the oral cavity.
Keywords
Dental polymer; MTT assay; Cytotoxicity test; Plastic instruments; Dental materials;
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