• International Journal of Oral Biology
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• v.40 no.4
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• pp.167-173
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• 2015

The purpose of this study was to investigate the antibacterial effect of the low temperature atmospheric plasma device with needle tip designed for easy approach to the oral cavity and root canal against Streptococcus mutans, Enterococcus faecalis and Candida albicans. The antibacterial activities evaluated by measuring clear zone of agar plate smeared with each bacteria after plasma treatment. To quantify antibacterial effects, dilution plate method was used. In addition, scanning electron microscope (SEM) was used for observation of changes in bacterial morphology. As treatment time of plasma increased, the clear zone was enlarged. The death rate was more than 99%. The SEM results showed that the globular shape of bacteria was distorted. These results suggest that needle tip plasma could be an innovative device for prevention of dental caries, and treatment of apical infection and soft tissue diseases.

• Journal of Oral Medicine and Pain
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• v.35 no.1
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• pp.31-39
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• 2010

This study is to observe the bacteriocidal effect of the Low Level Laser (LLL) against oral microorganisms which are related to the occurrence of periodontal diseases and oral malodors. The Porphyromonas gingivalis 2561 (P. gingivalis 2561) and Prevotella intermedia (Pr. intermedia) were treated with photosensitizing substance-toluidine blue O (TBO; C.I. 52040) and then radiated with the LLL which has 650nm wavelength for 1, 2, 3 and 5mins. continuously upon varying distances of 1, 2 and 3cm for each experimental groups. The results are as follows; 1. The P. gingivalis 2561 which was treated with TBO and then radiated with LLL at a distance of 3cm for 1min. showed 99.99% higher antibacterial effect in comparison to the experimental group treated only with TBO. 2. The Pr. intermedia which was treated with TBO and then radiated with LLL at a distance of 3cm for 1min. showed 99.8% higher antibacterial effect in comparison to the experimental group treated only with TBO. 3. The bacteriocidal effect of the P. gingivalis 2561 treated with TBO was found to gradually increase as the radiation time of LLL extended from 1min. to 3min. at 1min. intervals. 4. A slight decrease in bacteriocidal effect of the P. gingivalis 2561 was found as the radiation distance of LLL increased from 1cm to 3cm at 1cm intervals. 5. The bacteriocidal effect of the Pr. intermedia was found to slightly decrease as the radiation distance of LLL increased from 1cm to 3cm at 1cm. intervals. As the results shown above suggest, the bacteriocidal effect of LLL was found to increase as the radiation time extended and the distance shortened. Moreover, even the experimental group radiated with LLL at 3cm distance for 1min. which showed the lowest level of bacteriocidal effect, was found to have 99.8% higher bacteriocidal effect than the experimental group which was treated only with TBO and, therefore, this clearly shows the bacteriocidal effect of LLL against oral microorganisms. Thus, the use of LLL is thought to become very useful for suportive treatment for periodontitis and implantitis, and controlling oral malodors as long as it is used within the limits where there is no side effect.

• Journal of Oral Medicine and Pain
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• v.33 no.2
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• pp.159-166
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• 2008

An ideal anti-bacterial medication for oral infection requires selective effect on pathogens causing dental caries and periodontal disease but not on normal flora. In addition, it should be less toxic for human and even for environment. This study was to seek such a natural anti-bacterial medication and thus anti-bacterial effect of Hamamelis virginiana was evaluated. Many recent researches on the anti-bacterial effect of natural plant extract and essential oil have reported that natural products can be used as medication for prevention and restrainment of dental caries, halitosis and periodontitis. It has been also reported that Hamamelis virginiana has anti-bacterial effect on Porphyromonas gingivalis, Fusobacterium nucleatum, Capnocytophaga gingivalis, Veilonella parvula, Eikenella corrodens, Peprostreptococcus micros, and Actinomyces odontolyticus. This study evaluated anti-bacterial effect of Hamamelis virginiana on Streptoccoccus mutans, Haemophylus actinomycetemcomitans, and Klebsiella pneumoniae to expand its anti-bacterial effect on other important oral pathogens and eventually to develop its oral care products or apply to clinical purpose. In this study, anti-bacterial tests for antibiotic disk susceptibility, minimal inhibitory concentration and minimal bactericidal concentration were performed to evaluate anti-bacterial effect of Hamamelis virginiana against Streptoccoccus mutans, Haemophylus actinomycetemcomitans, and Klebsiella pneumoniae. The results showed that Hamamelis virginiana has anti-bacterial effect on all pathogen strains tested in this study and furthermore Hamamelis virginiana possesses bactericidal effect other than bacteriostatic effect on Streptoccoccus mutans, Haemophylus actinomycetemcomitans, Klebsiella pneumoniae. This study indicates that a natural anti-bacterial medication for oral diseases can be developed using Hamamelis virginiana.

• Journal of dental hygiene science
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• v.12 no.3
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• pp.287-294
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• 2012

The aim of this study was to investigate the effect of standardized oral health care program by dental hygiene school students. Target population was 100 University students who attended standardized oral health care program by a dental hygiene school students. Present study was conducted during first and second semester in 2011 at E dental hygiene school. We analysed students' oral health related knowledge, attitude, behavior, and self-oral hygiene care ability after the program. Univariate analysis, Chi-square test and paired t-test were conducted using SAS version 9.2. University students' who attended standardized oral health care program by dental hygiene school students oral health related knowledge, attitude, behavior, and self oral-hygiene care ability were significantly improved whether they have attended oral health related lectures or not(p<.05). It is recommended provide standardized oral health care program to university students by dental hygiene school students to promote their oral health related knowledge, attitude, behavior, and self-oral hygiene care ability.

• International Journal of Oral Biology
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• v.41 no.3
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• pp.105-111
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• 2016

${\beta}-carotene$ is present in carrots, pumpkins, and sweet potatoes. It suppresses many types of cancers by regulating cellular proliferation and apoptosis through a variety of mechanisms. However, the effects of ${\beta}-carotene$ on oral cancer cells have not been clearly established. The main goal of this study was to investigate the effects of ${\beta}-carotene$ on cell growth and apoptosis in oral cancer cells. Our results demonstrate that treatment with ${\beta}-carotene$ induced inhibition of cell growth, and that the effect was dependent on ${\beta}-carotene$ treatment time and concentration in KB cells. Furthermore, treatment with ${\beta}-carotene$ induced nuclear condensation and fragmentation in KB cells. ${\beta}-carotene$ promoted proteolytic cleavage of procaspase-3, -7, -8 and -9 with associated increases in the concentration of cleaved caspase-3, -7, -8 and -9. In addition, the level of cleaved PARP was increased by ${\beta}-carotene$ treatment in KB cells. These results suggest that ${\beta}-carotene$ can suppress cell growth and induce apoptosis in KB human oral cancer cells, and that it may have potential usefulness in anti-cancer drug discovery efforts.

• Journal of Korean society of Dental Hygiene
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• v.21 no.4
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• pp.403-412
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• 2021

Objectives: The objectives of this study were to assess oral health knowledge and behavior levels of community pharmacists as a step toward projecting them to play the role of oral health partners and to confirm pharmacists' willingness to participate in oral health education programs. Methods: t-test and one-way analysis of variance were performed to analyze the data, and correlation analysis was also performed. Results: The mean score for pharmacists' oral health knowledge was 7.29 out of 10. Of the 12 questions asked, the correct answer rate was highest for the question about the effect of smoking cessation on periodontal disease prevention; conversely, the correct answer rate was lowest for the question about the effect of taking medication for gingival infections on periodontal disease prevention. The mean score for pharmacists' oral health behavior was 2.97 out of 4 points. Of all oral health behaviors, brushing twice a day was the most practiced, whereas immediately visiting a dentist in case of an oral health issue was the least practiced. Pharmacists' oral health knowledge and behavior levels showed a weak positive correlation with their intention to participate in oral health education programs. Conclusions: Oral health education programs are necessary to improve community pharmacists' oral health knowledge and behavior.

• Journal of Korean society of Dental Hygiene
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• v.18 no.1
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• pp.125-138
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• 2018

Objectives: The purpose of the study was to investigate the effect of gender in the association between the oral symptoms experience and health behavior. Methods: The subjects were 54,219 adolescents selected from the web-based survey of the 11th (2015) Korean Youth Risk Behavior of Korean Center for Disease Control. Data were analyzed using SPSS 18.0. The subjects consisted of 27,198 male students(50.2%) and 27,021 female students(49.8%) from 400 middle schools and 400 high schools. Results: Multiple logistic regression analysis after adjustment to demographic characteristics and oral health behavior showed experience with drinking and smoking in adolescents increased oral symptoms experienced by both male and female students (OR = 1.3, 95% CI = 1.20-1.34 / OR =1.3, 95% CI = 1.18-1.33), (OR = 1.3, 95% CI = 1.24-1.40 / OR = 1.4, 95% CI = 1.43-1.30). But the difference was not significant between genders. In terms of diet, consumption of carbonated beverages, snacks and fast food saw an increase in oral symptoms experience both gender compared with those whose "No(weekly)" in particular to women, alternatively vegetable, fruit consumption(weekly) were having less intake adolescents increase oral symptoms experience than "time daily(weekly)" intake adolescents both boy and girl especially to girl. Conclusions: There was a correlation between oral symptom experiences and health behaviors. There was also slight differences between genders, with more effect shown on female students. Based on this study, proper and systematic education of oral health management should be carried out at schools.

• International Journal of Oral Biology
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• v.41 no.1
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• pp.1-8
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• 2016

OSCC is currently the most common malignancy of the head and neck, affecting tens of thousands of patients per year worldwide. Natural flavonoids from plants are potential sources for novel anti-cancer drugs. Icariin is the active ingredient of flavonol glycoside, which is derived from the medical plant Herba Epimedii. A metabolite of icariin, icariside II exhibits a variety of pharmacological actions, including anti-rheumatic, anti-depressant, cardiovascular protective, and immunomodulatory functions. However, the exact mechanism causing the apoptosis-inducing effect of icariside II in OSCC is still not fully understood. In the present study, we assessed the anti-cancer effect of icariside II in OSCC cell lines by measuring its effect on cell viability, cell proliferation, and mitochondria membrane potential (MMP). Icariside II treatment of OSCC cells resulted in a dose- and time-dependent decrease in cell viability. Hoechst staining indicated apoptosis in icariside II-treated HSC cells. Icariside II inhibited cell proliferation and induced apoptosis in HSC cells, with significant increases in all present parameters in HSC-4 cells. The results clearly suggested that icariside II induced apoptosis via activation of intrinsic pathways and caspase cascades in HSC-4 cell lines. The collective findings of the study suggested that Icariside II is a potential treatment for OSCC; in addition, the data could provide a basis for the development of a novel anti-cancer strategy.

• Biomolecules & Therapeutics
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• v.1 no.2
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• pp.220-225
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• 1993

Effect of BR-900317 on the angiotensin I-induced pressor response in pithed rats and the effects of its single oral administration on plasma angiotensin converting enzyme (ACE) activities in normotensive rats and on the cardiovascular system in hypertensive model rats (SHR, RHR), were compared with those of captopril. BR-900317 attenuated the angiotensin I-induced pressor effects in pithed rats. In a single oral dose administration study, BR-900317 inhibited the plasma ACE activities in a dose-dependent fashion. Duration of the action of BR-900317 was similar to that of captopril. BR-900317 produced antihypertensive effect in spontaneously hypertensive rats and dose-dependent antihypertensive effect in 2-kidney Goldblatt hypertensive rats without affecting heart rate. These results suggest that the main mechanism of the antihypertensive effect of BR-900317 is the suppression of angiotensin II production due to the inhibition of the ACE.

• Journal of Korean society of Dental Hygiene
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• v.16 no.4
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• pp.617-623
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• 2016

Objectives: The purpose of this study is to investigate the effect of health behavior on oral health of Korean adolescents. Methods: The study subjects were 1,071 adolescents of 13-18 years old from Korea National Health and Nutrition Examination Survey (KNHANES) 2010-2012. Male students were 54.5% and female students were 45.5%. The study consisted of two groups: 13-15 years old and 16-18 years old groups. Data were analyzed by SAS 9.13 version. Results: Those who ingested alcohol had gingival bleeding in 41.0% and 31.0% in those who did not. Those who did not have regular oral examinations had 38.5% of gingival bleeding while those with regular oral examination had 29.7% of gingival bleeding (p<0.01). Students between 16 to 18 years old without oral care products tended to have more gingival bleeding than those who use auxiliary oral hygiene devices (OR=2.658, 95% CI=1.327-5.324). Conclusions: The oral health management of adolescents is closely related to health behavior. Cessation of alcohol ingestion and smoking is very important to improve the adolescent oral health.