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Antimicrobial Effect of Low Temperature Atmospheric Plasma against Oral Pathogens

  • Kim, Young Min (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Choi, Byul Bo Ra (Department of Dental Hygiene, Dongseo University) ;
  • Park, Sang Rye (Department of Dental Hygiene, Kyungnam College of Information & Technology) ;
  • Kim, Ji Young (Department of Dental Hygiene, Kyungnam College of Information & Technology) ;
  • Kim, Gyoo Cheon (Department of Oral Anatomy, School of Dentistry, Pusan National University)
  • Received : 2015.10.13
  • Accepted : 2015.11.24
  • Published : 2015.12.31

Abstract

The purpose of this study was to investigate the antibacterial effect of the low temperature atmospheric plasma device with needle tip designed for easy approach to the oral cavity and root canal against Streptococcus mutans, Enterococcus faecalis and Candida albicans. The antibacterial activities evaluated by measuring clear zone of agar plate smeared with each bacteria after plasma treatment. To quantify antibacterial effects, dilution plate method was used. In addition, scanning electron microscope (SEM) was used for observation of changes in bacterial morphology. As treatment time of plasma increased, the clear zone was enlarged. The death rate was more than 99%. The SEM results showed that the globular shape of bacteria was distorted. These results suggest that needle tip plasma could be an innovative device for prevention of dental caries, and treatment of apical infection and soft tissue diseases.

Keywords

References

  1. Banerjee A, Watson TF, Kidd EA. Dentine caries excavation: A review of current clinical techniques. Br Dent J. 2000;188:476-482. doi: 10.1038/sj.bdj.4800515.
  2. Kidd EA, Role of chlorhexidine in the management of dental caries. Int Dent J. 1991;41:279-286.
  3. Han X, Klas M, Liu Y, Stack MS, Ptasinska S. DNA damage in oral cancer cells induced by nitrogen atmospheric pressure plasma jets. Appl Phys Lett. 2013;23:233703. doi:10.1063/1.4809830.
  4. Kalghatgi SU, Fridman G, Cooper M, Nagaraj G, Peddinghaus M, Balasubramanian M, Vasilets VN, Gutsol AF, Fridman A, Friedman G. Mechanism of blood coagulation by nonthermal atmospheric pressure dielectric barriers discharge plasma. IEEE Tans Plasma Sci. 2007;5:1559-1566. doi: 10.1109/TPS.2007.905953.
  5. Isbary G, Stolz W, Shimizu T, Monetti R, Bunk W, Schmidt HU, Morfill GE, Klämpfl TG, Steffes B, Thomas HM, Heinlin J, Karrer S, Landthaler M, Zimmermann JL. Cold atmospheric argon plasma treatment may accelerate wound healing in chronic wounds: Results of an open retrospective randomized controlled study in vivo. Clin Plasma Med. 2013;1:25-30. doi: 10.1016/j.cpme.2013.06.001.
  6. Lee HW, Kim GJ, Kim JM, Park JK, Lee JK, Kim GC. Tooth bleaching with nonthermal atmospheric pressure plasma. J Endod. 2009;35:587-591. doi: 10.1016/j.joen.2009.01.008.
  7. Sladek REJ, Filoche SK, Sissons CH, Stoffels E. Treatment of Streptococcus mutans biofilms with a nonthermal atmospheric plasma. Lett Appl Microbiol. 2007;45:318-323. doi: 10.1111/j.1472-765X.2007.02194.x.
  8. Marchal F, Robert H, Merbahi N, Fontagné-Faucher C,Yousfi M, Romain CE, Eichwald O, Rondel C, Gabriel B. Inactivation of gram-positive biofilms by low-temperature plasma jet at atmospheric pressure. J Phys D: Appl Phys. 2012;45:345202. doi: 10.1088/0022-3727/45/34/345202.
  9. Collins CH, Lyne PM. Microbiological methods. 5th ed. London: Butterworths; 1984.
  10. Bystrom A, Claesson R, Sundqvist G. The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endod Dent Traumatol. 1985;1:170-175. doi:10.1111/j.1600-9657.1985.tb00652.x.
  11. Distel JW, Hatton JF, Gilespie MJ. Biofilm formation in medicated root canals. J Endod. 2002;28:689-93. doi:10.1097/00004770-200210000-00003.
  12. Dunavant TR, Regan JD, Glickman GN, Solomon ES, Honeyman AL. Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilims. J Endod. 2006;32:527-531. doi: 10.1016/j.joen.2005.09.001.
  13. Akpan A, Morgan R. Oral candidiasis. Postgrad Med J. 2002;78:455-459. doi: 10.1136/pmj.78.922.455.
  14. Gutteridge JM, Quinlan GJ, Kovacic P. Phagomimetic action of antimicrobial agent. Free Radic Res. 1998;28:1-14. doi:10.3109/10715769809097871.
  15. Grabowski LR. Pulsed corona in air for water treatment [dissertation]. Eindhoven: Eindhoven University of Technology; 2006.
  16. Park HS, Min KJ, Cha GC, Song JW, Son JC. Antimicrobial activities against oral microbes and growth-inhibitory effect on oral tumor cell by extract of Paeonialacti flora. Kor J Env Hlth. 2007:33;21-29. doi: 10.5668/JEHS.2007.33.1.021.
  17. Byström A, Sundqvist G. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J. 1985;18:35-40. doi: 10.1111/j.1365-2591.1985.tb00416.x.
  18. White RR, Hays GL, Janer LR. Residual antimicrobial activity after canal irrigation with chlorhexidine. J Endod. 1997;23:229-231. doi: 10.1016/S0099-2399(97)80052-0.
  19. Sjogren U, Figdor D, Spangberg L, Sundqvist G. The antimicrobial effect of calcium hydroxide as a short-term intra canal dressing. Int Endod J. 1991;24:119-125. doi:10.1111/j.1365-2591.1991.tb00117.x.
  20. Matsuo T, Shirakami T, Ozaki K, Nakanishi T, Yumoto H, Ebisu S. An immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions. J Endod. 2003;29:194-200. doi: 10.1097/00004770-200303000-00008.
  21. Pan J, Sun K, Liang Y, Sun P, Yang X, Wang J, Zbang J, Zbu W, Fang J. Cold plasma therapy of a tooth root canal infected with Enterococcus faecalis biofilms in vitro. J Endod. 2013;39:105-110. doi: 10.1016/j.joen.2012.08.017.
  22. Kantheti LPC, Reddy BVR, Ravikumar S, Anuradha CH, Chandrasekhar P, Rajeswari MR. Isolation, identification, and carriage of candida species in PHLAs and their correlation with immunological status in cases with and without HAART. J Oral Maxillofac Pathol. 2012;16:38-44. doi: 10.4103/0973-029X.92971.
  23. Galle F, Sanguinetti M, Colella G, Onofrio VD, Torelli R, Rossano F, Liguori G. Oral candidosis: characterization of a sample of recurrent infections and study of resistance determinants. New Microbiol. 2011;34:379-389.
  24. Koban I, Matthes R, Hubner NO, Welk A, Meisel P, Holtfreter B, Sietmann R, Kindel E, Weltmann K, Kramer A, Kocher T. Treatment of Candida albicans biofilms with lowtemperature plasma induced by dielectric barrier discharge and atmospheric pressure plasma jet. New J Phys. 2010;12:073039. doi: 10.1088/1369-2630/12/7/073039.
  25. Maisch T, Shimizu T, Isbary G, Heinlin J, Karrer S, Klämpfl TG, Li Y, Morfill G, Zimmermann JL. Contact-free inactivation of Candida albicans biofilm by coldatmospheric air plasma. J Appl Environ Microbiol. 2012;78:4242-4247. doi: 10.1128/AEM.07235-11.
  26. Burkes EJ, Hoke J, Gomes E, Wolbarsht M. Wet versus dry enamel ablation by Er:YAG laser. J Prosthetic Dentistry. 1992;67:847-851. doi: 10.1016/0022-3913(92)90599-6.
  27. Zach L, Cohen G. Pulp response to externally applied heat. Oral Surg Oral Med Oral Pathol. 1965;19:515-530. doi: 10.1016/0030-4220(65)90015-0.
  28. Lu X, Ye T, Cao Y, Sun Z, Xiong Q, Tang Z, Xiong Z, Hu J, Jiang Z, Pan Y. The roles of the various plasma agents in the inactivation of bacteria. J Appl Phys. 2008;104:053309. doi: 10.1069/1.2977674.
  29. Vleugels M, Shama G, Deng XT, Greenacre E, Brocklehurst T, Kong MG. Atmospheric plasma inactivation of biofilmforming bacteria for food safety control. IEEE Trans Plasma Sci. 2005;33:824-828. doi: 10.1109/TPS.2005.844524.
  30. Pelletier J. La sterilisation par le procede plasma. Agressologie. 1993;33:105-110.
  31. Perni S, Shama G, Hobman JL, Lund PA, Kershaw CJ, Hidalgo-Arroyo GA, Penn CW, Deng XT, Walsh JL, Kong MG. Probing bactericidal Mechanisms induced by cold atmospheric plasma with Escherichia coli mutants. Appl Phys Lett. 2007;90:073902. doi: 10.1063/1.2458162.
  32. Laroussi M. Non-thermal decontamination of biological media by atmospheric pressure plasma: review, analysis and prospects. IEEE Trans Plasma Sci. 2002;30:1409-1415. doi: 10.1109/TPS.2002.804220.