• 한국축산식품학회지
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• 제39권5호
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• pp.844-861
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• 2019

Over the last few years, marine environment was found to be a source of surplus natural products and microorganisms with new bioactive secondary metabolites of interest which can divulge nutritional and biological impact on the host. This study aims to assess the possible, inherent and functional probiotic properties of a novel probiotic strain Enterococcus durans F3 (E. durans F3) isolated from the gut of fresh water fish Catla catla. Parameters for evaluating and describing the probiotics described in FAD/WHO guidelines were followed. E. durans F3 demonstrated affirmative results including simulated bile, acid and gastric juice tolerance with exhibited significant bactericidal effect against pathogens Staphylococcus aureus, Salmonella Typhi, Escherichia coli and Pseudomonas aeruginosa. This can be due to the enterocin produced by E. durans F3 strain, which was resolute by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) gel with amplification of the anticipated fragment of a structural gene; enterocin A, followed by antibiotic susceptibility assessment. Effective antioxidant potentiality against ${\alpha}$-diphenyl-${\alpha}$-picrylhydrazyl free radicals including lipase, bile salt hydrolase activity with auto-aggregation and cell surface hydrophobicity was similarly observed. Results are proving the potentiality of E. durans F3, which can also be used as probiotic starter culture in dairy industries for manufacturing new products that imparts health benefits to the host. Finding the potent and novel probiotic strains will also satisfy the current developing market demand for probiotics.

• 약학회지
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• 제50권4호
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• pp.220-227
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• 2006

The therapeutic effect of tissue cultured root of mountain ginseng (Panax ginseng) (tcMG) on 2,3,7,8-tetrachlorodaibenzo-p-dioxin (TCDD) induced toxicity in rat was investigated. The rats were assigned into three groups (10 rats/group), control, TCDD exposed group and tcMG treated group after TCDD exposed. $50\;{\mu}g/kg$ of TCDD was injected by i.p. for TCDD exposed group and 30 mg/kg of tcMG saponin was administered for 4 weeks by oral gavage for tcMG treated group. The weights of body, spleen, kidney, thymus, testes and epididymides were decreased in the single TCDD treatment. However these organs was significantly recovered by tcMG saponin except thymus (p<0.05). tcMG decreased the level of hepatic demage maker enzymes, AST and ALP. It also lowered total cholesterol and triglyceride. The level of serum triglyceride was significantly decreased in tcMG saponin treated group compared with the control. Histopathological examination revealed morphological change in the liver spleen, thymus and testes of TCDD treated rats. However they were relatively well preserved in the tcMG treatment group. In conclusion, TCDD induced toxicity was some repaired by tcMG. tcMG may be useful for prevention and treatment of TCDD induced toxicity.

• 비교문화연구
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• 제35권
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• pp.7-34
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• 2014

This essay explores how Leslie Marmon Silko's Ceremony and Storyteller encourage human's sympathetic relationship with the nonhuman animal-Other, paying attention to her posthumanist voices against anthropocentric mistreatment of animals which is inseparable from white Americans' environmental and racio-ethnic subjugation of nature and Natives in the colonialist history of the United States. As a way of dissolving the problematic anthropocentrism and embracing the animal-Other as a fellow creature, Silko employs and transforms Native American oral tradition in her own idiosyncratic posthumanist storytelling. In order to highlight the ethico-political examination of the animal issue in her storytelling, this essay refers to contemporary posthumanist thinkers such as Jacques Derrida, Giorgio Agamben, and Gilles Deleuze who are all in their own ways critically engaged with Western metaphysical anthropocentrism. Arguably, in a similar vein with the posthumanist critics, Silko disrupts the mischievous hierarchical opposition of humans/animals that have directly or obliquely warranted violence against the animal-Other. In order to demonstrate Silko's ethico-politicality concerning the animal issue, this essay inquires her critical perception of humans' misunderstanding (or misbehavior) toward animals in terms of the suffering and death of animals. Besides, Silko's posthumanist storytelling of the animal's gaze (as Derrida notes as an event of revealing human aporia and vulnerability) and "in-between" (as a reification of crossing the boundary of humans/animals) is discussed with the exemplification of Tayo's encounter with a mountain lion and a bear-man Shush. The posthumanist approach to thinking about the animal-Other in Ceremony and Storyteller would shed light on the ethico-political significances of Silko's storytelling in our time in peril of losing the tie between humans and nonhuman animals.

• 기록학연구
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• 제49호
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• pp.299-325
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• 2016

이 글에서는 마을기록물의 중요성과 유형 및 소장처, 수집과 보존, 활용 방안에 대해서 살펴보았다. 마을기록물은 주민의 일상 활동을 실증하며 마을의 역사와 문화를 규명하는 데 기여하며 구술의 불완전성을 보완하는 마을문화의 자원이다. 마을기록물을 수집하기 위해서는 그것의 유형과 소장처, 존재방식에 대한 이해가 있어야 한다. 마을기록물은 자치조직의 활동에서 생산된 기록물, 주민의 개인기록물, 마을 안팎에 존재하며 마을사를 증거해 줄 수 있는 기록물을 전체적으로 고려해야 한다. 마을기록물의 수집방법은 정기적 수집과 비정기적 수집 그리고 일반적 수집과 적극적 수집으로 분류할 수 있다. 마을기록물은 마을기록관에 보존하는 것을 지향해야 한다. 마을기록물은 주민의 삶의 질과 정서함양에 도움을 주며 책 자, 포스터, 달력, 홍보물, 상징물, 학술 및 교육, 전시, 디지털콘텐츠, 마을사의 사료 및 히스토리마케팅의 자료로 활용될 수 있다.

• Journal of Microbiology and Biotechnology
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• 제29권5호
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• pp.704-712
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• 2019

Although nanometric dead Lactobacillus plantarum has emerged as a potentially important modulator of immune responses, its underlying mechanism of action has not been fully understood. This study aimed to identify the detailed biochemical mechanism of immune modulation by micronized and heat-treated L. plantarum LM1004 (MHT-LM1004, <$1{\mu}m$ in size). MHT-LM1004 was prepared from L. plantarum LM1004 via culture in a specifically designed membrane bioreactor and heat treatment. MHT-LM1004 was shown to effectively induce the secretion of $TNF-{\alpha}$ and IL-6 and the mRNA expression of inducible nitric oxide synthase (iNOS). MHT-LM1004 enhanced the expression of TLR-2, phosphorylation of MAPKs (ERK), and nuclear translocation of $NF-{\kappa}B$ in a dose-dependent manner. Oral administration of MHT-LM1004 ($4{\times}10^9$ or $4{\times}10^{11}cells/kg$ mouse body weight) increased the splenocyte proliferation and serum cytokine levels. These results suggested that MHT-LM1004 effectively enhances early innate immunity by activating macrophages via the TLR-2/MAPK/$NF-{\kappa}B$ signalling pathway and that this pathway is one of the major routes in immune modulation by the Lactobacillus species.

• 대한두개안면성형외과학회지
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• 제20권2호
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• pp.89-93
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• 2019

Background: Prophylactic antibiotic administration after surgery for a nasal bone fracture is performed due to concerns about infection-related complications, such as, toxic shock syndrome. To evaluate the validity and efficacy of antibiotic use, we compared the results obtained and the bacterial profiles of nasal packing materials in patients that underwent closed reduction for a nasal bone fracture with or without prophylactic antibiotic administration. Methods: Thirty consecutive patients with a nasal bone fracture, but without an open wound, that underwent closed reduction during March to August 2017 were included in the present study. Fifteen of these 30 patients were randomly assigned to a control group, members of were administered postoperative intravenous antibiotics once at the day of surgery and then oral antibiotics for 4 days. The other 15 patients were assigned to an experimental group and not administered any antibiotic postoperatively. Antibiotic ointment was not applied to nasal packing in either group. Nasal packing was removed on postoperative day 4 in all cases. Removed nasal gauze packings were culture tested and strains identified in the two groups were compared. Results: Bacterial strain types cultured from packings were similar in the experimental and control groups and no patient showed signs of clinically significant infection. Conclusion: The findings of this study suggest postoperative prophylactic antibiotic use is not clinically required after closed reduction of a nasal bone fracture. Furthermore, the non-use of postoperative antibiotics is biologically beneficial, as it reduces the occurrence of resistant strains and medical costs, and is more convenient for patients.

• Journal of Veterinary Science
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• 제19권6호
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• pp.759-770
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• 2018

Fowl adenovirus (FAdV) is distributed worldwide and causes economic losses in the poultry industry. The objectives of this study were to determine the hexon and fiber gene changes in an attenuated FAdV isolate from Malaysia in specific pathogen-free chicken embryonated eggs (SPF CEE) and its infectivity in commercial broiler chickens. SPF CEE were inoculated with 0.1 mL FAdV inoculum via the chorioallantoic membrane (CAM) for 20 consecutive passages. The isolate at passage 20 (E20), with a virus titer of $10^{8.7}TCID_{50}/mL$ ($TCID_{50}$, 50% tissue culture infective dose), was inoculated (0.5 mL) into one-day-old commercial broiler chicks either via oral or intraperitoneal routes. The study demonstrated that 100% embryonic mortality was recorded from E2 to E20 with a delayed pattern at E17 onwards. The lesions were confined to the liver and CAM. Substitutions of amino acids in the L1 loop of hexon at positions 49 and 66, and in the knob of fiber at positions 318 and 322 were recorded in the E20 isolate. The isolate belongs to serotype 8b and is non-pathogenic to broiler chickens, but it is able to induce a FAdV antibody titer. It appears that molecular changes in the L1 loop of hexon and the knob of fiber are markers for FAdV infectivity.

• 영미문화
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• 제14권2호
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• pp.207-232
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• 2014

Scholars have considered Chaucer's House of Fame mostly as an ars poetica, in which the poet explores new poetic principles and subject matters, while making few attempts to understand the poem in its historical and social contexts. Investigating the nature of the "tidings" that Chaucer suggests as the new source of his poetic inspiration, this paper argues that the house of Rumor was modeled after late fourteenth century English society that experienced increased appetite for news. The political upheaval during the period from the English Rising in 1381 to the reign of Henry IV in the early fifteenth century produced an unprecedented amount of written and oral propaganda. The proliferation of seditious rumors as well as protests and promulgations during this period indicates how seriously medieval society was engaged with the circulation of news. Particularly, the case of John Shirle in 1381 and the legend about the survival of Richard II demonstrate the subversive power of medieval rumor that often served as a political discourse with which people expressed their oppositions to government. Conspicuous in the activities of both the government and late medieval political protestors was the extensive use of writing. The posting of bills in public places continued until the fifteenth century, when such activities became so common and dangerous that the government had to issue proclamations forbidding the circulation of such seditious writings. The number of extant royal proclamations, written protests, and pamphlets demonstrates that already in the late fourteenth and fifteenth centuries the notion of a discursive public space began to emerge. Whether written or orally transmitted, news and rumor circulated in late medieval England, creating a social space in which people shared their political opinions before the introduction of the early modern print culture. In The House of Fame Chaucer calls attention to the subversiveness of rumor, its potential as a public discourse, and the power of written communication in creating truth in order to appropriate these characteristics for his English poems.

• 한국축산식품학회지
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• 제42권5호
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• pp.903-914
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• 2022

Probiotics are currently considered as one of tools to modulate immune responses under specific clinical conditions. The purpose of this study was to evaluate whether oral administration of three different probiotics (Lactiplantibacillus plantarum CJLP243, CJW55-10, and CJLP475) could evoke a cell-mediated immunity in immunodeficient mice. Before conducting in vivo experiments, we examined the in vitro potency of these probiotics for macrophage activation. After co-culture with these probiotics, bone marrow derived macrophages (BMDMs) produced significant amounts of proinflammatory cytokines including interleukin-6 (IL-6), IL-12, and tumor necrosis factor-α (TNF-α). Levels of inducible nitric oxide synthase (inos) and co-stimulatory molecules (CD80 and CD86) were also upregulated in BMDMs after treatment with some of these probiotics. To establish an immunocompromised animal model, we intraperitoneally injected mice with cyclophosphamide on day 0 and again on day 2. Starting day 3, we orally administered probiotics every day for the last 15 d. After sacrificing experimental mice on day 18, splenocytes were isolated and co-cultured with these probiotics for 3 d to measure levels of several cytokines and immune cell proliferation. Results clearly indicated that the consumption of all three probiotic strains promoted secretion of interferon-γ (IFN-γ), IL-1β, IL-6, IL-12, and TNF-α. NK cell cytotoxicity and proliferation of immune cells were also increased. Taken together, our data strongly suggest that consumption of some probiotics might induce cell-mediated immune responses in immunocompromised mice.

• Pediatric Infection and Vaccine
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• 제29권3호
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• pp.173-178
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• 2022

Streptococcus intermedius는 입안, 상부 호흡기, 위장관 등에 정상 상재균 중 하나로 존재하는 그람 양성 산소내성 혐기성 알균이다. 면역저하나 심장질환자에서 외상 또는 침습적 치료 후에 발생하는 기회 감염의 원인이 될 수 있다. 저자들은 최근 침습적 치료나 외상 등 특이 과거력이 없이 건강했던 7세 소아에서 S. intermedius에 의해 발생한 엉덩관절 화농 관절염을 경험하였기에 보고하고자 한다. 환자는 내원 3주전부터 왼쪽 엉덩관절 통증이 있었으며, 본원 내원 당일부터 발열이 발생하였다. 엉덩관절 자기공명영상에서 왼쪽 엉덩관절의 급성 화농 관절염으로 진단한 후 수술적 배농을 시행하였고, 혈액과 흡인 관절액에서 S. intermedius가 배양되었다. 수술 후 2주간의 ceftriaxone 정맥주사 후 3주간의 amoxicillin 경구 항생제로 치료한 후 재발없이 안정된 상태를 보이고 있다.