• Parasites, Hosts and Diseases
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• 제61권3호
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• pp.282-291
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• 2023

Despite the recent progress in public health measures, malaria remains a troublesome disease that needs to be eradicated. It is essential to develop new antimalarial medications that are reliable and secure. This report evaluated the pharmacokinetics and antimalarial activity of 1,2,6,7-tetraoxaspiro[7.11]nonadecane (N-89) using the rodent malaria parasite Plasmodium berghei in vivo. After a single oral dose (75 mg /kg) of N-89, its pharmacokinetic parameters were measured, and t_1/2 was 0.97 h, T_max was 0.75 h, and bioavailability was 7.01%. A plasma concentration of 8.1 ng/ml of N-89 was maintained for 8 h but could not be detected at 10 h. The dose inhibiting 50% of parasite growth (ED₅₀) and ED₉₀ values of oral N-89 obtained following a 4-day suppressive test were 20 and 40 mg/kg, respectively. Based on the plasma concentration of N-89, we evaluated the antimalarial activity and cure effects of oral N-89 at a dose of 75 mg/kg 3 times daily for 3 consecutive days in mice harboring more than 0.5% parasitemia. In all the N-89-treated groups, the parasites were eliminated on day 5 post-treatment, and all mice recovered without a parasite recurrence for 30 days. Additionally, administering oral N-89 at a low dose of 50 mg/kg was sufficient to cure mice from day 6 without parasite recurrence. This work was the first to investigate the pharmacokinetic characteristics and antimalarial activity of N-89 as an oral drug. In the future, the following steps should be focused on developing N-89 for malaria treatments; its administration schedule and metabolic pathways should be investigated.

• Journal of Korean Academy of Oral Health
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• 제42권4호
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• pp.109-117
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• 2018

Objectives: The purpose of this study was to evaluate the customized oral health promotion program for North Korean defectors (NKDs). Methods: Using a pre-survey comprising phases 1-5 of the PRECEDE-PROCEED model, a customized oral health promotion program was created. The participants, a total of 129 NKDs, were allocated to the experimental group (n=64; 43 females and 21 males) or the control group (n=65; 46 females and 19 males). After the interventions, phases 7 to 9 of the PRECEDE-PROCEED model were executed to determine the effect of the program. Results: The results for phase 7 revealed high satisfaction with the program, with an average of 2.89 out of 3 points. In phase 8, concerning the impact evaluation, it was found that oral health beliefs and oral health knowledge were improved, with statistically significance differences before and after the experiment. Phase 9, concerning the outcome evaluation, showed that there were statistically significant differences in the average within groups before and after education in oral health beliefs (OHB), O'Leary index scores, and CPI. In terms of the difference between the groups, there were statistically significant improvement in OHB, O'Leary index scores, and CPI after the education program. Conclusions: The oral health promotion program developed in this study has made a positive contribution to improve the oral health status of NKDs.

• Archives of Pharmacal Research
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• 제26권1호
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• pp.89-94
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• 2003

In vivo clearance of BMS-182874 was primarily due to metabolism via stepwise N-demethylation. Despite in vivo clearance approached ca 50％ of the total liver plasma flow, BMS-182874 was completely bioavailable after oral administration in rats. Saturable first-pass metabolism and the role of extrahepatic tissue were evaluated as possible reasons for complete oral bioavailability despite extensive metabolic clearance. Pharmacokinetic parameters were obtained after an intravenous and a range of oral doses of BMS-182874 in rats. Bile and urine were collected from bile-duct cannulated (BDC) rats and the in vivo metabolic pathways of BMS-182874 were evaluated. Pharmacokinetics of BMS-182874 were also compared in nephrectomized (renally impaired) vs. sham-operated control rats. Oral bioavailability of BMS-182874 averaged 100％, indicating that BMS-182874 was completely absorbed and the first-pass metabolism (liver or intestine) was negligible. The AUC and C/sub max/ values increased dose-proportionally, indicating kinetics were linear within the oral dose range of 13 to 290 mmole/kg. After intravenous administration of BMS-182874 to BDC rats, about 2％ of intact BMS-182874 was recovered in excreta, indicating that BMS-182874 was cleared primarily via metabolism in vivo. The major metabolite circulating in plasma was the mono-N-desmethyl metabolite and the major metabolite recovered in excreta was the di-N-desmethyl metabolite. In vivo clearance of BMS-182874 was significantly reduced in nephrectomized rats. These observations suggest saturable first-pass metabolism is unlikely to be a mechanism for complete oral bioavailability of BMS-182874. Reduced clearance observed in the nephrectomized rats suggests that extrahepatic tissues (e.g., kidneys) may play an important role in the in vivo clearance of xenobiotics that are metabolized via N-demethylation.

• Journal of Periodontal and Implant Science
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• 제28권4호
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• pp.659-678
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• 1998

본 연구에서는 한국인 성인성 치주염의 관련세균 중에서 구강 스피로헤타의 분포를 조사하기 위하여 배양하지 않고도 구강 스피로헤타를 분리할 수 있는 16S rRNA에 의거한 올리고뉴클레오타이드 소식자를 사용하였다. 성인성 치주염 환자 29명을 대상으로 한 사람당 6mm 이상의 탐침 깊이를 보이는 부위 4곳(실험군)과 3mm 이하의 탐침 깊이를 보이는 건강한 부위 1곳(대조 1군), 건강한 치주조직을 가진 학생 20명을 대상으로 한 사람당 5부위로부터 치은연하 치태를 채취한 뒤(대조 2군) 중합효소연쇄반응과 점상블롯보합결합법을 시행하였다. 소식자로는 구강 스피로헤타의보편 소식자 및 현재 배양이 되는 구강 스피로헤타중에서 T. denticola, T. pectinovorum, T. socranskii, T. vincentii, T. maltophilum에 대한 종 특이 소식자 TDEN, TPEC, TSOC, TVIN, TMAL과 현재 배양이 되지않은 구강스피로헤타중에서 I-VII군에 대한 군 특이 소식자 TRE I-TRE VII을 이용하여 다음과 같은 결론을 얻었다. 1. 위상차 현미경으로 본 결과는 실험군, 대조 1군에서 각기 91.37%, 14.28%의 구강스피로헤타가 관찰되었으며 대조 2군에서는 관찰되지 않았다. 2. 보편 소식자를 사용한 경우는 실험군, 대조 1군, 대조 2군에서 각기 98.27%, 46.42%, 22.0%의 구강 스피로헤타가 관찰되었다. 3. 특이 소식자를 사용한 경우는 실험군, 대조 1군, 대조 2군에서 각기 95.68%, 35.71%, 19.0%의 구강 스피로헤타가 관찰되었다. 4. 종 특이 소식자를 사용한 경우는 T. socranskii가 가장 많이 관찰되었으며 (81.89%), 그다음이 T. maltophilum(50.0%), T. vincentii(36.20%), T. denticola(13.79%)순이었고, 군 특이 소식자를 사용한 경우는 TREIV(85.34%), TRE II(77.58%), TREI(56.89%), TRE III(25.86%), TREVI(5.17%), TRE V(2.58%) 순이었다. 5. T. vincentii는 치주염에 이환된 부위에서만 관찰되었고, 건강한 부위에서는 관찰되지 않았다. 6. T. pectinovorum과 VII군에 속하는 구강스피로헤타는 어느 표본에서도 관찰되지 않았다. 이상의 결과에서 16S rRNA에 의거한 올리고뉴클레오타이드 소식자로 구강 스피로헤타의 성인성 치주염과의 연관성과 분리되지 않은 구강 스피로헤타를 확인하였으며, 인종 및 치주염의 형태에 따른 구강 스피로헤타의 분포 차이, T. vincentii의 병원성, 치료 전후의 구강 스피로헤타의 분포 변화등의 보다 세분화된 연구가 필요하다고 생각된다.

• Journal of Oral Medicine and Pain
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• 제39권3호
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• pp.83-89
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• 2014

Purpose: Chronic nicotine administration induce various effects in whole organs of the body; however, little is known about salivary gland. In the present study, we pursued the links between systemic nicotine and the histomorphological changes of the salivary gland in mice. Methods: Twenty-five C57BL6 mice were allocated into two groups. The control group (n=9) received distilled water only for 8 weeks by gavage. The experimental nicotine group (n=16) was administered nicotine $5{\mu}g/g$ with distilled water. Animals were sacrificed at 8 weeks; then, submandibular glands were excised and processed for histologic evaluation. Volumetric changes in acinar cells were evaluated by H&E staining. The expression of calponin-positive myoepithelial cells and Ki-67-positive proliferating acinar cells were evaluated by immunohistochemistry. Results: The nicotine group showed significantly decreased number of calponin-positive myoepithelial cell process compared with the control group. There were no significant differences in average volume of acinar cell and the number of Ki-67-positive acinar cells between both groups. Conclusions: These findings suggested that chronic nicotine administration may cause decreased function of myoepithelial cells in submandibular glands of mice, and these can partly explain xerostomic conditions in chronic smokers.

• Biomolecules & Therapeutics
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• 제6권1호
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• pp.45-49
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• 1998

The pharmacokinetic properties and antiinflammatory activity of 3% ketoprofen lotion (ID-lotion), formulated with poloxamer 407, were evaluated using rats. For the pharmacokinetic study, the lotion, at the dose of 4.5 mg/kg, was applied on the dorsal skin of rats and the drug concentration in plasma was determined using an HPLC method. As references, ketoprofen suspended in saline was administered orally, and E-lotion, which is a 3% ketoprofen lotion in the Japanese market was applied transdermally. Following the transdermal application of ID-lotion and E-lotion, $C_{max}$ were 316 $\pm$22.3 ng/ml and 163 $\pm$ 12.2 ng/ml, respectively, at the same Tma of 2 hours postdose, while $C_{max}$ and $T_{max}$ after oral administration of the drug were 1,030$\pm$89.1 ng/ml and 0.25 hours, respectively. Relative bioavailabilities of ID-lotion and I-lotion were 69.3% and 34.2%, respectively. The antiinflammatory activity of the two 3% ketoprofen lotions was evaluated with carrageeneninduced edema method after 50 mg of the lotions was applied on the paw of rats. ID-lotion showed 67.6% inhibition of the edema formation, while I-lotion showed 34.\\\\`r%. The calculated ED5o after transdermal application of ID-lotion was 2.5 mg/kg, while that after oral administration was 7.0 mg/kg. Based on these results, the relative equiponderal availability of ID-lotion was 296% compared to the oral administration of ketoprofen.n.n.n.

• 한국학교ㆍ지역보건교육학회지
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• 제18권3호
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• pp.55-68
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• 2017

Objectives: Republic of Korea has entered an aged society, recently. As chronic diseases increase, elderly inpatients has been increasing and they have used caregivers for convenience. Accordingly, this study aims to investigate the oral health knowledge and attitude of caregivers on oral health management of elderly inpatients, which would affect the general health and quality of life. Methods: A survey questionnaire was distributed to the caregiver(n=165), at a neurosurgery or orthopedic hospital inpatient ward in Seoul, Gyeonggi-do from April 1 to 14, 2017. We analyzed the data with descriptive statistics analysis, chi-squared analysis and logistic regression analysis by using SPSS 23.0. Results: The response rate to the questionnaire was 92%. Oral health management provided by caregivers to inpatients in the elderly was prevention of oral diseases(92.8%), tooth brushing (89.5%), use of oral care products(68.4%), denture cleaning(90.8%) and oral cleaning(90.8%). Frequency analysis of oral health management in accordance with the educational needs of caregivers was all confirmed statistically significant(p<0.05). In the logistics regression model, oral health knowledge was significantly associated with low oral cleaning(OR=1.58) and oral health attitude was significantly associated with high denture cleaning(OR=1.29) and oral cleaning(OR=1.28) after adjustment for other covariates including gender, age, certification, education level, ward, working years. Conclusions: It is necessary to expand the scope and improve the quality of education that can change the attitudes and behaviors of the caregivers on the oral health management of the elderly on the basis of the current oral health education.

• 한국학교보건학회지
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• 제21권1호
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• pp.33-41
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• 2008

Purpose : The purpose of this study was to provide the basic data for p lanning oral health education in elementary schol. Methods conducted using a postal, self-administered questionnaire. A questionnaire containing 19 questions about oral health education which was provided by health teachers in elementary schol was used for data colle ction. Total response rate was 64.3% (119 out of 185). Results : Oral health education except one through textbooks was conduc of which taught oral health themselves. The contents of oral he alth education through special clas was mainly focusing on the tothbrushing method, dental caries, and the use of fluo ride. Acording to the grades clasified by the level of education, the order was the 3rd, 1st, and 2nd grade, which mea ns that the lower grades got more training than the h was acquired through the internet (72.2%), health-related organizations or academies (51.5%). Also, materi als for oral health education were obtained from health-related organizations or academies (67%), self-productio n (49.5%). According to the data during the past 5 years it was found that 13.4% health teachers had oral health-related that more traing neded to be conducted from 89.7% health tea chers. The most dificult problem in oral health education were insufficient time (56.7%). Conclusion : For effective oral health education, there needs a developmen t of targeted goal to achieve a systemic oral health education for each grades as wel as a ned for as health teachers to eficiently acquire knowledge and materials for oral health education, it is necessary to hold regular workshops for health teachers, and develop and distribute appro priate educational materials.

• International Journal of Oral Biology
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• 제36권3호
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• pp.123-128
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• 2011

The notion that dental amalgam is a potential source of mercury exposure remains a controversial issue. However, there are few epidemiological analyses that have addressed whether this occurs in children. We aimed in our current study to identify the relationship between dental amalgam filling surfaces and the blood mercury levels in a cohort of 711 South Korean children aged between 8-9 years. Oral examinations were conducted to detect the number of amalgam filling surfaces on the teeth of these individuals. Blood samples were also taken from these children to assess the levels of mercury accumulation in the body. The amalgam filling surfaces were classified into four groups based on their number: 0 (n = 368), 1-5 (n = 219), 6-10 (n = 89), and 11+ (n = 35). The blood mercury levels in the children with more than 10 amalgam surfaces was 0.47 ${\mu}g$/L higher on average than those with no amalgam surfaces after adjusting for the frequency of fish or seafood consumption, age, and gender (P < 0.05). We found from our data that a higher number of dental amalgam fillings correlated with a higher blood mercury level in Korean children. Further studies are needed to investigate whether these elevated mercury levels exert neurotoxic or nephrotoxic effects.

• 치과방사선
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• 제7권1호
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• pp.16-18
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• 1977

The purposes of this investigation was to determine the appearance rate of nutrient canals and the aspect of alveolar bone trabecular patterns. 1949 subjects, averages 23 years old, who have normal occlusion were used. The film used in this investigation was full mouth roentgenographic survey. The obtained results were as follows: 1. In the trabecular pattern, coarse form was 35.17% and fine one was 64.14% in both jaws. 2. The appearance rate of nutrient canals was much higher in mandibular anterior segment(89.13%) than in the maxillary posterior segment(0.86%). 3. In the trabecular forms, small trabecular space and horizontal relation appeared in the mandible, and vertical relation in the maxilla.