• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.60 no.3
• /
• pp.300-307
• /
• 2015

In order to develop optimum paddy-upland rotation system, we evaluated the 1st and the 2nd upland growth and yield characteristics of foxtail millet, proso millet, sorghum rotated from paddy field and rice rotated from upland in paddy-upland rotation. Average number of ears per hill was 3.3 in the 2nd upland cultivation. The value was greater by 1 ear as compared to 1st upland cultivation (2.2 ears per hill). In average yield per 10a, the 2nd upland cultivation showed 220.3 kg, 23% increased yield compared to the 1st upland cultivation (179 kg per 10a). In average number of ears per hill, the 2nd upland cultivation showed 8.3 ears, increased 4 ears compared to the 1st upland cultivation (4.2 ears per hill). In average yield per 10a, the 2nd upland cultivation showed 152.8 kg, 16.8% increased yield compared to the 1st upland cultivation (130.8 kg per 10a). In average days from seeding to heading of 5 sorghum varieties, there were no significant difference between the 1st (68.6 days) and the 2nd (67.4 days) upland cultivation rotated from paddy field. In the average number of grains per ears, the 2nd upland cultivation showed 2,931.6 grains per ear, 12% increased compared to the 1st upland cultivation (2,619.6 grains per ears). Average yield per 10a of sorghum in the 2nd upland cultivation showed 242.3 kg, 4.6% increased compared to the 1st upland cultivation (231.7 kg per 10a). In growth and yield characteristics of rice in paddy-upland rotation, culm length in paddy-upland-paddy plot showed 82.9 cm, 7.3 cm longer compared to the continuous rice paddy field (75.6 cm). Ear length was also 1 cm longer than that of the continuous rice paddy field. In average number of ears per hill, paddy-upland-paddy plot showed 25.0 ears, 4.3 ears more than that of the continuous rice paddy field (20.7 ears per hill). In average yield of rice per 10a, the paddy-upland-paddy rotation plot showed 526.8 kg, 9.8% higher yield compared to the continuous rice paddy field (479.9 kg per 10a).

• Microbiology and Biotechnology Letters
• /
• v.37 no.2
• /
• pp.118-124
• /
• 2009

Esterase EM2L8 gene isolated from deep sea sediment was expressed in Escherichia coli BL21 (DE3) and the esterase activity of the cell-free extract was assayed using p-nitrophenyl butyrate-spectrophotometric method. Its optimum temperature was $40-45^{\circ}C$ and 45% activity of the maximum activity was retained at $15^{\circ}C$. The activation energy at $15-45^{\circ}C$ was calculated to be 4.9 kcal/mol showing that esterase EM2L8 was a typical cold-adapted enzyme. Enzyme activity was maintained for 6 h and 4 weeks at $30^{\circ}C$ and $4^{\circ}C$, respectively. When each ethanol, methanol, and acetone was added to the reaction mixture to 15% concentration, enzyme activity was maintained. In the case of DMSO, enzyme activity was kept up to 40% concentration. (S)-4-Chloro-3-hydroxy butyric acid is a chiral intermediate for the synthesis of Atorvastatin, a hyperlipemia drug. When esterase EM2L8 (40 U) was added to buffer solution (1.2 mL, pH 9.0) containing ethyl-(R,S)-4-chloro-3-hydroxybutyrate (38 mM), it was hydrolyzed into 4-chloro-3-hydroxy butyric acid with a rate of $6.8\;{\mu}mole/h$. The enzyme hydrolyzed (S)-substrate more rapidly than (R)-substrate. When conversion yield was 80%, e.e.s value was 40%. When DMSO was added, hydrolysis rate increased to $10.4\;{\mu}mole/h$. The plots of conversion yield vs e.e.s in the presence or absence of DMSO were almost same, implying that the reaction enantioselectivity was not changed by the addition of DMSO. Taken together, esterase EM2L8 had high activity and stability at low temperatures as well as in various organic solvents/aqueous solutions. These properties suggested that it could be used as a biocatalyst in the synthesis of useful pharmaceuticals.

• KSBB Journal
• /
• v.8 no.5
• /
• pp.495-503
• /
• 1993

To investigate the characteristics of growth and oil production of peppermint cells during a batch culture, cells derived from peppermint callus was cultivated in an air bubble reactor. During the batch culture, effects of inoculum size, abiotic stress, yeast elicitor, and two stage culture on the cell growth, the productivity of oleolesin, and the formation of flavor components were determined and also the sugar concentrations and kinetics of cell growth were analyzed. Among the various sizes of inoculum, the culture with 2.0% packed cell volume inoculum showed the optimum condition for cell growth in the proposed bioreactor, and the cell yield and essential oil production reached to 5.7g/1 and 0.109g/1, respectively. When the abiotic stress of daily 8hr dark and $10^{\circ}C$ cold treatments were given to the culture cell growth decreased but essential oil production increased to 0.546g/l. In a modified Lin-Staba medium in which 100mg/l yeast extract as an elicitor was added to the culture, the cell growth and oil production increased, and menthol content was 22.5% of oil. In the two stage culture, in which the basic culture conditions of 27$^{\circ}C$, light, and without elicitor were employed during the first six days followed by the second stage with daily 8hr treatment of cold and dark condition, and also with yeast extract as an elicitor, cell growth decreased after eight days, essential oil production was not increased, and menthol was not detected. Dry cell yield was 0.38g dry cell/g sugar and specific growth rate was 0.25 day-1. The major terpenoid in the oil was not the menthol but pulegone and piperitone, precursors of menthol were accumulated. However, when yeast elicitor was added, menthol was produced to the level of 22.5% which was the highest value in the peppermint cell culture reported so far.

• Food Science and Preservation
• /
• v.12 no.6
• /
• pp.522-528
• /
• 2005

Effects of packaging and storage temperature on the quality and shelf life of mungbean sprouts(vigna radiata (L.) Wilczek) were studied Mungbean sprouts were packaged in polypropylene films(PP) and oriented polypropylene films(OPP) with 200 g, 250 g, and 300 g and stored at $4^{\circ}C,\;8^{\circ}C$ and $12^{\circ}C$, respectively. The deterioration of quality of mungbean sprouts during storage was caused by wilting of hypocotyl, abscission of cotyledon and softening of tissue. Total weight loss never exceeded $1\%$ and no visible signs of shrivelling of mungbean sprouts were observed. At $4^{\circ}C,\;30{\mu}m$ of OPP film per 250 g mungbean sprouts provided the optimal atmosphere composition(i.e. $3\%\;\O_2\;and\;5\%\;CO_2$). A shelf life of 6 days was achieved with these conditions. Hardness of hypocotyl, when deterioration in freshness began, was about 1,027.2 g when considerably deteriorated Hunter b value was 13 in deteriorated hypocotyl, vs. 11 for hypocotyl of fresh mungbean sprouts was accelerated by fluctuating storage temperature by the increment of storage time. It also was found that the optimum shelf life period was estimated to be 6, 2 and 2 days for 4, 8 and $12^{\circ}C$, respectively.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.37 no.7
• /
• pp.921-926
• /
• 2008

Wild grapes contain lots of organic acids, vitamins and inorganic salts compared to other fruits. Also, it has known to have excellent effects on preventing cancer and heart disease. Wild grapes are supposed to be superior material for fermentation of wine. Tannin contents of wine, which is an important functional factor in flavor of wine, were enhanced to develop quality of Korean wild grape wine. In this study, we investigated tannin extraction conditions and analyzed quality properties of tannin-enhanced wild grape wine. The most suitable tannin extraction condition for enhancing tannin of wild grape wine was extraction of tannin from green tea using spirits at 4oC. For producing high quality wild grape wine, added concentration and time of tannin extract were 6.5 mg/mL before wild grape wine fermentation. Tannin contents of tannin-enhanced wild grape wine was $7.4{\pm}0.05\;mg/mL$. Quality properties of tannin-enhanced wild grape wine fermented under optimized fermentation conditions were analyzed. pH, acidity and alcohol contents were $3.69{\pm}0.01$, $0.95{\pm}0.01%$ and $12.2{\pm}0.03%$, respectively. Total sugar, tannin, polyphenol and resveratrol contents of tannin-enhanced wild grape wine were $60.00{\pm}1.15\;mg/mL$, $79.50{\pm}0.55\;mg/mL$, $7.40{\pm}0.05\;mg/mL$ and $5.00{\pm}0.11\;mg/mL$, respectively showing significantly higher value than that of commercial wine. Production of high quality wild grape wine is expected with the establishment of optimum fermentation condition and tannin-enhancing process of wild grape wine.

• Korean Journal of Environmental Agriculture
• /
• v.19 no.3
• /
• pp.194-200
• /
• 2000

The amount of swine wastewater reaches about $197,000m^3$ per day at live-stock houses in the whole country. A half of the swine wastewater resources are too small to be restricted legally. This untreated wastewater causes the eutrophication in the water bodies. In case of swine wastewater treatment, the solid-liquid separation must be performed because feces(solid phase) and urine(liquid phase) have large differences in nitrogen and phosphorus concentration. It is necessary to assess exactly the concentration of the pollutants in swine wastewater for planning the wastewater treatment facilities. A full-scale operation was carried out in K city and the plant is consists of conventional plant, the supplementary flocculation basin of chemical treatment process and $anaerobic{\cdot}aerobic$ basin for nitrogen removal. The improved full-scale swine wastewater treatment plant removed the $1,500{\sim}3,000mg/l$ of total-nitrogen(T-N) to 120mg/l of T-N and $131{\sim}156mg/l$ of total-phosphorus(T-P) to $0.15{\sim}1.00mg/l$ of T-N. Accordingly, as a results of operational improvement, the removal efficiencies of T-N and T-P were over $92{\sim}96%$, 99%, respectively. The continuous supply of organic carbon sources and the state of pH played important roles for the harmonious metabolism in anaerobic basin and the pH value of anaerobic basin maintained at about 9.0 for the period of the study.

• Journal of Bio-Environment Control
• /
• v.20 no.3
• /
• pp.211-215
• /
• 2011

This study aimed to determine optimum leaves per shoot in 'Campbell Early' grape through investigating changes of fruit quality after veraison 70~75 berries of first cluster and 35~40 berries of second cluster at 30 days after full bloom. Soluble solids content (SSC) increased dramatically during 28 days after veraison and afterwards it was stabilized. 15 leaves per shoot treatment showed fast increase of SSC from early veraison and reached to over 16.0oBx at 28 days after veraison. No considerable increase of SSC were occurred on either 5 or 0 leaves per shoot treatments after veraison. Titratable acidity (TA) was decreased continuously during 28 days of berry ripening in the all treatments. In 15 leaves per shoot treatment, TA decreased fast during first 7 days after veraison and continuously decreased to 0.5% by harvest time. While, 5 and 0 leaves per shoot treatments showed TA decrease slightly only at the early veraison. Hunter L and b values of 15 leaves per shoot treatment decreased fast during first 7 days of veraison, and gradually decreased by 14 days, whereas Hunter a value increased fast during the first 7 days from veraison. Total anthocyanin content in 15 leaves per shoot treatment increased fast from 7 days of veraison and the increase became slow after 28 days of veraison. Other treatments showed no significant difference from veraison to harvest time.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.30 no.2
• /
• pp.115-120
• /
• 2010

This study was carried out to determine the forage production and quality of Italian ryegrass (IRG) and forage barley sown in early spring in Suwon, 2009. The five treatments used in this experiment were IRG Kowinearly (early maturity), IRG Kowinmaster (medium maturity), Yuyeon barley, Kowinearly + Yuyeon mixture, and Kowinmaster + Yuyeon mixture. The lodging was observed in IRG, but no lodging was found IRG + barley mixtures. The heading date of Kowinearly and Kowinmaster were 16 May and 22 May, respectively, and that of barley was 13 May. The dry matter (DM) percentage at harvest was 22.2~27.6%. The forage quality among treatments were similar, but the crude protein (CP) content of IRG was higher than that of barley, and in vitro DM digestibility was a little low in Kowinmaster. The yields of DM, CP and digestible DM were high in Kowinearly + Yuyeon barley mixtures as a 13,816 kg, 1,384 kg and 10,387 kg per ha, respectively (p<0.05). In conclusion, the mixture cultivation of IRG and forage barley was very effective, because of preventing of IRG lodging, increasing of forage yield, and stable production of forages. The optimum harvest date for silage manufacture of IRG and barley sown in early spring was recommended early June instead of May.

• Korean Journal of Soil Science and Fertilizer
• /
• v.16 no.2
• /
• pp.131-155
• /
• 1983

Though it has been widely known the nitrogen effects are influenced by soils, varieties, and mineral nutrients in the rice culture, few analyses in relation to the factors increasing nitrogen effect have been studied in Korea. The effects of potassium and silica on the factors increasing nitrogen effects in paddy soils were investigated in accordance with soil improvement practices and nitrogen application methods for the cultivated varieties. The results obtained are as follows. 1. For 413 paddy fields, the yield from soils without nitrogen application ranged from 200 to 850kg/10a and that from nitrogen application did 350 to 1,051kg/10a. The yield increament by nitrogen application varied 50 to 650kg/10a depending on soils. 2. Soil chemical characteristics for high yield were different between with nitrogen and without nitrogen application. In the without nitrogen application, however, contents of organic matter, phosphorous, potassium and calcium of high yield soils were lower than those of low yield, while the available silica content was higher in the former. 3. The yield increased with nitrogen application up to 22.4kg/10a and thereafter it decreased. These phenomena were supposed to be not be decrease of nitrogen uptake but by lowered silica uptake. 4. Clay soil incorporation, deep plough, and inorganic constituents control such as Ca, Mg, and $Sio_2$ were effective as soil improvement praitices. It was appeared that increases of silica content and Ca/Mg ratio were important to increase nitrogen effects. 5. For the correlation between yield and yield components, it was high between yield and panicle in low nitrogen level and so was it between grain yield and ripening rate in high nitrogen. 6. In the urea and super granule urea application plot, recovery rate of nitrogen by plant and soil was high and yield was remarkable high. 7. Regardless of fertilizer types such as ammonium sulfate and urea, the residual nitrogen was about 4kg/10a in both plots of 5.8 and 11.6kg/10a. N applied. 8. The potassium application to soil enhanced the nitrogen efficiency. It was more effective in low potassium soil. 9. Optimum pH value for gel formation in the 4% sodium silicate solution was approximately 6.6. 10. It was suggested that silica could affect to rice plant growth as the inorganic and organic chemical components.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.25 no.2
• /
• pp.125-130
• /
• 2005

This study was conducted from March 16 to July 6 in 2004 at Jeju Island to investigate the influences of sowing dates(on March 16, March 26, April 5, April 15 and April 25) on creeping bentgrass vegetation. The result obtained were summarized as follows; Plant height was 22.7 cm at March 16 planting. It was longest but after that planting, plant height gradually shorted. Then it was shortest at April 25 planting(16.6 cm). Root length and Minolta SPAD-502 chlorophyll reading value were directly proportional plant height response. Leave and root weight were greatest at March 16 planting. It were 1,373 kg /10a and 2,374 kg /10a, respectively. These weight decreased gradually as planting was delayed from March 16 to April 25. Degree land cover and density of creeping bentgrass were $98.0\%$ and $99.3\%$, respectively, at March 16. After that planting they were decreased ($97.5\%$, $98.7\%$). But degree land cover and density of weed tended to increased gradually as the planting was delayed. The number of weed species were increased from March 16 to April 25. It showed increase that Poa annua, Stellaria media and Chenopodium album var. centrorubrum(at March 16 planting), Poa annua, Digitaria adscendens and Chenopodium album var. centrorubrum(at March 26 planting), Digitaria adscendens, Chenepodium album var. centrorubrum and Stellaria media(at April 5 planting), Digitaria adscendens, Stellaria media and Chenopodium album var. centrorubrum(at April 15 planting), Digitaria adscendens, Polygonum hydropiper, Chenopodium album var. centrorubrum(at April 25 planting). Based on the these findings, optimum sowing date for growth of creeping bentgrass seems to be about early seeding in atmospheric phenomena and volcanic ash soils of Jeju island.