• BMB Reports
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• v.35 no.3
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• pp.313-319
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• 2002

N-Acetyl-$\beta$-D-hexosaminidase ($\beta$-HexNAc'ase) (EC 3.2.1.52) was purified from rice seeds (Oryza sative L. var. Dongjin) using ammonium sulfate (80%) precipitation, Sephadex G-150, CM-Sephadex, and DEAE-Sephadex chromatography, sequentially. The activities were separated into 7 fractions($F_1-F_7$) by CM-Sephadex chromatography. Among them, F6 was further purified to homogeneity with a 13.0% yield and 123.3 purification-fold. The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37.4 kDa on Sephacryl S-300 gel filtration. The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-$\beta$-D-hexosaminide (pNP-GlcNAc) and p-nitrophenyl-N-acetyl-$\beta$-D-hexosaminide (pNP-GalNAc) as substrates, which are typical properties of $\beta$-HexNAc'ase. The ratio of the pNP-GlcNAc'ase activity to the pNP-GalNAc'ase activity was 4.0. However, it could not hydrolyze chitin, chitosan, pNP-$\beta$-glucopyranoside, or pNP-$\beta$-glucopyranoside. The enzyme showed $K_m$, $V_{max}$ and $K_{cat}$ for pNP-GlcNAc of 1.65 mM, $79.49\;mM\;min^{-1}$, and $4.79{\times}10^6\;min^{-1}$, respectively. The comparison of kinetic values for pNP-GlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site. The purified enzyme exhibited optimum pH and temperature for pNP-GlcNAc of 5.0 and $50^{\circ}C$, respectively. The enzyme activity for pNP-GlcNAc was stable at pH 5.0-5.5 and $20-40^{\circ}C$. The enzyme activity was completely inhibited at a concentration of 0.1 mM $HgCl_$ and $AgNO_3$, suggesting that the intact thiol group is essential for activity. Chloramine T completely inhibited the activity, indicating the possible involvement of methionines in the mechanism of the enzyme.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.5B
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• pp.331-343
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• 2012

In this study, the site investigation for fish was performed in the 15 km of Hongcheon river including Oancheon stream. The river ecosystem health was evaluated using the field data for fish. The field survey was carried out at 9 sites, 4 times from August to November 2011. The ecological diversity, including dominance, evenness, and richness and the ecological health using IBI and QHEI were evaluated. The result shows that the mean IBI in the 9 sites is in good-common condition, but the downtown section has a common-worse condition. The result evaluated by QHEI shows optimum-good condition. Also, the habitat suitability index for Pseudopuntungia tenuicorpa, which is one of endangered species, was evaluated, and then the environment flow was calculated by using the PHABSIM model. The previous research in the literature reports that Acheilognathus signifer, one of the endangered species, inhabited in Hongchen river. However, the existence of Acheilognathus signifer was not found in the recent research and this study. Thus, the physical habitat condition for Acheilognathus signifer was evaluated using the field data in the previous study. Also, the habitat improvement for Acheilognathus signifer in Hongcheon river was proposed.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.6
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• pp.1161-1167
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• 2016

In this study, an attempt was made to conduct a case study on the development of ground expansive displacement due to lack of bearing capacity of original ground in spite of applying reinforcement treatments that intended to enhance the stability of big size high-speed rail tunnel in large fault zone. For the purpose of this, in-situ measurements made in the middle of excavation stage were analyzed in order to characterize ground responses and numerical analysis was performed to evaluate the effectiveness of reinforcement technique such as elephant foot method applied for this site via comparing with field monitoring measurements. In addition, further numerical studies were carried out to investigate the influence of leg pile installation angle and length, which is one of types of elephant foot method. The results revealed that the optimum condition for the leg pile installation is to maintain 45 degree of installation angle along with 6 meter of embedment depth.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.691-694
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• 2014

Background: Tumor length in patients with esophageal cancer (EC) has recently received great attention. However, its prognostic role for EC is controversial. The purpose of our study was to characterize the prognostic value of tumor length in EC patients and offer the optimum cut-off point of tumor length by reliable statistical methods. Materials and Methods: A retrospective analysis was conducted on 71 consecutive patients with EC who underwent surgery. ROC curve analysis was used to determine the optimal cut-off point for tumor length, measured with a handheld ruler after formalin fixation. Correlations between tumor length and other factors were surveyed, and overall survival (OS) rates were compared between the two groups. Potential prognostic factors were evaluated by univariate Kaplan-Meier survival analysis. A P value less than 0.05 was considered significant. Results: There were a total of 71 patients, with a male/female divide of 43/28 and a median age of 59. Characteristics were as follows: squamous/adenocarcinoma, 65/6; median tumor length, 4 (0.9-10); cut-off point for tumor length, 4cm. Univariate analysis prognostic factors were tumor length and modality of therapy. One, three and five year OS rates were 84, 43 and 43% for tumors with ${\leq}4cm$ length, whereas the rates were 75, 9 and 0% for tumors >4 cm. There was a significant association between tumor length and age, sex, weight loss, tumor site, histology, T and N scores, differentiation, stage, modality of therapy and longitudinal margin involvement. Conclusions: Future studies for modification of the EC staging system might consider tumor length too as it is an important prognostic factor. Further assessment with larger prospective datasets and practical methods (such as endoscopy) is needed to establish an optimal cut-off point for tumor length.

• Microbiology and Biotechnology Letters
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• v.16 no.4
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• pp.310-315
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• 1988

Two isoenzymes of chorismate mutase(E.C.5.4.99.5) designated as chorismate mutase I(CM I) and chorismate mutase II(CM II), were detected and partially purified from a sp. of intrasporangium isolated from soil. CM I and CM II had pH optima of pH 6.5 and 8.0, respectively and showed the same temperature optimum of 45$^{\circ}C$. The activation energy of the enzymatic reaction was estimated to be 14.7kcal/ mole with CM I and 10.8kcal/mole with CM II. The affinity of isoenzyme CM I for substrate(Km= 1.35mM) was almost the same level as that of CM II(Km = 1.22mM). Both isoenzymes were stable at pH values ranged from pH 6.5 to 9.0, but rapidly denaturated at temperatures above 45$^{\circ}C$. CM II was activated about 7$^{\circ}C$ of its activity by $Ba^{++}$ or $Mg^{++}$ while CM I was slightly inhibited by the same metal ions. Thiol compounds were found not to be necessary for stability of the two enzymes but Co$^{++}$ and EDTA had a little stabilizing effect on CM II only. p-Chloromercuribenzoate strongly inactivated the activities of both enzymes but the reducing agents such as dithiothreitol and L-cysteine protected them against the pCMB inhibition.

• The korean journal of orthodontics
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• v.34 no.6 s.107
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• pp.526-536
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• 2004

This study was undertaken to determine the effect of a 2.2mg/Kg/day intraoral administration of NaF on the amount of root resorption and osteoclastic activity during or after a rapid maxillary expansion procedure. Ten puerile female dogs were divided into two groups: a control group and a NaF-treated group. A fixed type maxillary expansion device was delivered to all dogs. The appliance was activated twice daily throughout a 20-day period, causing a 5-mm expansion of maxillary bone. After the expansion procedure, the animals were sacrificed at days 0, 15, 30, 45, and 60 of the retention period. The buccal surface of the root of each maxillary canine was examined by means of a surface electron microscope (SEM). Using SEM, web-like resorption lacunae were observed on the bone or the tooth surface at the site of osteoclastic activity; these observations were verified by histological methods. No peculiar resorption lacunae were found in the apical tip of the roots of either the control group or the NaF-treated group animals. The NaF-treated retention group was found to have less resorption lacunae formation on day 45 and day 60. The preventative effect of NaF on resorption lacunae formation on the surface of the bone covering the anchor tooth was confirmed. Larger areas of resorption lacunae were found on the surface of the bone covering the canines in the control group animals, as compared to those of the NaF-treated group, especially on day 30 and day 60. Using SEM, the present study revealed a difference between the control group and the NaF-treated group in the prevalence and the size of the resorption lacunae formation on the cemental root surface. The preventative effect of NaF on bone resorption was confirmed. Further studies concerned with the optimum concentration of NaF that has an effect in vivo are necessary.

• Korean Journal of Microbiology
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• v.45 no.2
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• pp.200-207
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• 2009

In this study, the characterization of purified erythritol 4-phosphate dehydrogenase, key enzyme of erythritol biosynthesis, produced by Penicillium sp. KJ81 was investigated. Optimum production conditions of erythritol 4-phosphate dehydrogenase was 1 vvm areration, 200 rpm agitation, at $37^{\circ}C$ for 8 days in the medium containing 30% sucrose, 0.5% yeast extract, 0.5% $(NH_4)_2SO_4$, 0.1% $KH_2PO_4$, and 0.05%$MgCl_2$. Erythritol 4-phosphate dehydrogenase was purified through ultrafiltration and preparative gel electrophoresis from cell extract of Penicillium sp. KJ81. This enzyme was especially active on erythrose 4-phosphate with 1.07 mM of Km value. It gave a single band on native polyacrylamide gel electrophoresis and an isoelectric point of 4.6. The enzyme had an optimal activity at pH 7.0 and $30^{\circ}C$. It was stable between pH 4.0 and 9.0, and also below $30^{\circ}C$. The enzyme activity was completely inhibited by 1mM $Cu^{2+}$ and 1 mM $Zn^{2+}$, but was not significantly affected by other cations tested. This enzyme was inactivated by treatment of tyrosine specific reagent, iodine and tryptophan specific reagent, N-bromosuccinimide. The substrate of the enzyme, erythrose 4-phosphate showed protective effect on the inactivation of the enzyme by both reagents. These results suggest that tryptophan and tyrosine residues are probably located at or near active site of the enzyme.

• Journal of the Korean Recycled Construction Resources Institute
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• v.2 no.1
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• pp.19-25
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• 2014

The purpose of this study is to establish experimentally the mechanical properties of fiber reinforced concrete using kenaf dosages and propose the usable method of kenaf fiber in the concrete industry as natural fiber materials. Kenaf fiber help make the concrete strength including tensile and flexural stronger, more resistant to plastic and drying shrinkage, less amount of carbon dioxide because of having a rough surface and excellent tensile strength of fiber and improving the concrete's corrosion resistance. It is to select the kenaf dosages of 4 cases (0, 0.3, 0.6 and $0.9kg/m^3$ and perform various tests including slump, air content, plastic and drying shrinkage, flexural and tensile strength for fiber reinforced concrete. The results of this study are as follows : In case of increasing kenaf fiber dosages, show the slump decrease and air content increase, also take effect results for increasing concrete strength including flexural and tensile, decreasing plastic and drying shrinkage. therefore, considered test results and cost, the optimum dosages of kenaf fiber is proposed about $0.6kg/m^3$ and need to study on the site application considering concrete quality and another compared tests.

• Journal of the Korean Wood Science and Technology
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• v.38 no.1
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• pp.66-74
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• 2010

This study was carried out to investigate the optimum condition for sap exudation of Acer mono Max. tree in a site of Mt. Baekun, Kwangyang city, Korea. Amount of sap exudation, air temperature, relative air humidity and tree diameter at breast height (DBH) were monitored for the period of January 5 through March 28, 2008, and correlation analysis of several factors affecting on sap exudation was carried out. As the diameter of Acer mono at breast height increased, the amount of sap was linearly proportional. Sap exudation initiated at February 18, and occurred intensively in the period of February 28 through March 10, resulting in 84% of total sap amount by volume. During sap exudation, the minimum temperature was averaged at $-2.4{\pm}1.5^{\circ}C$ and the maximum at $6.0{\pm}1.8^{\circ}C$, while there was no sap exudation whenever temperature was below or above $0^{\circ}C$ all the day long. The maximum temperature, range of temperature and the maximum, minimum and mean humidities in air were significant factors affecting on amount of sap. The maximum air temperature had the highest correlation coefficient with 0.768 (P < 0.01) and was also considered as the principal factor by partial-correlation analysis. These results showed that sap exudation required daily air-temperature fluctuation from below to above $0^{\circ}C$, and the amount of sap was strongly dependent on the highest daily-temperature and DBH of tree.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2005.06a
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• pp.137-144
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• 2005

Silica in primary system combines with an alkali grammatical particle metal and forms the zeolite layer which is hindering the heat transfer on the surface of the cladding. Zeolite layer becomes the cause of the damage in this way. The problems of the NPP's primary system have been issued steadily by EPRI. Through a series of experiments of the laboratory scale, we confirmed the applicability of NF membrane for silica removal, as silica rejection rate of NF membrane is about $60\;{\sim}\;70\%$ and boron rejection rate is about $10\;{\sim}\;20\%$. We accomplished a site experiment about four NF membranes manufactured by FilmTec and Osmonics Inc. In experiment using 400L of SFP water, when operation pressure is $10kg_{f}/cm^2$, we confirmed that the silica rejection rate of NF90-2540 manufactured by FilmTec Inc. is about $98\%$, boron rejection rate is about $43\%$. The silica rejection rate of NF270-2540 is about $38\%$, boron rejection rate is about $3.5\%$. Afterward, through additional experiments, such as long term characteristic experiments, we are going to design a optimum NF membrane system for silica removal.