• Korean Journal of Medicinal Crop Science
• /
• v.9 no.1
• /
• pp.76-82
• /
• 2001

This study was conducted to find out the optimum ranges of soil physical properties for wasabi growth by the relationship of soil physical properties and plant growth. Soil bulk density and hardness were higher in decomposition of granite and river sand than hydroball. This root distribution of surface layer was higher in decomposition of granite and river sand than hydroball. Growth characteristics and yield were higher in hydroball than decomposition of granite and river sand. In inlet site, the marketable rhizome weight in decompasition of granite, river sand, hydroball were 298kg/10a, 401kg/10a, 766kg/10a, respectively. But outlet, the weight in three soils were 251kg/10a, 256kg/10a, 633kg/10a, respectively.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.1
• /
• pp.89-97
• /
• 2015

Bacillus subtilis HK176 with high fibrinolytic activity was isolated from cheonggukjang, a Korean fermented soyfood. A gene, aprE176, encoding the major fibrinolytic enzyme was cloned from B. subtilis HK176 and overexpressed in E. coli BL21(DE3) using plasmid pET26b(+). The specific activity of purified AprE176 was 216.8 ± 5.4 plasmin unit/mg protein and the optimum pH and temperature were pH 8.0 and 40℃, respectively. Error-prone PCR was performed for aprE176, and the PCR products were introduced into E. coli BL21(DE3) after ligation with pET26b(+). Mutants showing enhanced fibrinolytic activities were screened first using skim-milk plates and then fibrin plates. Among the mutants, M179 showed the highest activity on a fibrin plate and it had one amino acid substitution (A176T). The specific activity of M179 was 2.2-fold higher than that of the wild-type enzyme, but the catalytic efficiency (k_cat/K_m) of M179 was not different from the wild-type enzyme owing to reduced substrate affinity. Interestingly, M179 showed increased thermostability. M179 retained 36% of activity after 5 h at 45℃, whereas AprE176 retained only 11%. Molecular modeling analysis suggested that the 176^th residue of M179, threonine, was located near the cation-binding site compared with the wild type. This probably caused tight binding of M179 with Ca²⁺, whichincreased the thermostability of M179.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.12
• /
• pp.2087-2097
• /
• 2016

Most cold-adapted enzymes possess higher $K_m$ and $k_{cat}$ values than those of their mesophilic counterparts to maximize the reaction rate. This characteristic is often ascribed to a high structural flexibility and improved dynamics in the active site. However, this may be less convincing to cold-adapted metabolic enzymes, which work at substrate concentrations near $K_m$. In this respect, cold adaptation of a shikimate kinase (SK) in the shikimate pathway from psychrophilic Colwellia psychrerythraea (CpSK) was characterized by comparing it with a mesophilic Escherichia coli homolog (EcSK). The optimum temperatures for CpSK and EcSK activity were approximately $30^{\circ}C$ and $40^{\circ}C$, respectively. The melting points were $33^{\circ}C$ and $45^{\circ}C$ for CpSK and EcSK, respectively. The ${\Delta}G_{H_2O}$ (denaturation in the absence of denaturing agent) values were 3.94 and 5.74 kcal/mol for CpSK and EcSK, respectively. These results indicated that CpSK was a cold-adapted enzyme. However, contrary to typical kinetic data, CpSK had a lower $K_m$ for its substrate shikimate than most mesophilic SKs, and the $k_{cat}$ was not increased. This observation suggested that CpSK may have evolved to exhibit increased substrate affinity at low intracellular concentrations of shikimate in the cold environment. Sequence analysis and homology modeling also showed that some important salt bridges were lost in CpSK, and higher Arg residues around critical Arg 140 seemed to increase flexibility for catalysis. Taken together, these data demonstrate that CpSK exhibits characteristics of cold adaptation with unusual kinetic parameters, which may provide important insights into the cold adaptation of metabolic enzymes.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.19 no.3
• /
• pp.313-319
• /
• 2009

The blasting has a lot of economic efficiency and speediness but it can damage to a neighbor structure, a domestic animal and a cultured fish due to the blasting vibration, then the public grievance is increased. Therefore, we need to manage the blasting vibration efficiently. The prediction of the correct vibration velocity is not easy because there are lots of different kinds of the scale of blasting vibration and it has a number of a variable effect. So we figure the optimum line through the least-squares regression by using the vibration data measured in hard rock blasting and compared with the design vibration prediction equation. As a result, we confirm that the vibration estimated in this paper is bigger than the design vibration prediction equation in the same charge and distance. If there is a Gaussian normal distribution data on the left-right side of the least squares regression, then we can estimate the vibration prediction equation on reliability 50%(${\beta}=0$), 90%(${\beta}=1.28$), 95%(${\beta}=1.64$). 99.9%(${\beta}=3.09$). As a result, it appears to be suitable that the reliability is 99% at the transverse component, the reliability 95% is at the vertical component, the reliability 90% is at the longitudinal component and the reliability is 95% at the peak vector sum component.

• International Journal of Highway Engineering
• /
• v.17 no.5
• /
• pp.67-73
• /
• 2015

PURPOSES : This study was conducted to derive the optimum mixing ratio of phosphorescent pigment for the development of phosphorescent line marking. METHODS: In this study, we utilized a literature review and case study methodology, to describe the domestic and foreign state of practice for the production and mixing of phosphorescent pigment for use in line marking. The optimal mixing ratio was derived by comparing the reduction in luminance over time for the various phosphorescent pigment mixing ratios identified in the literature. In addition, performance and construction characteristics were analyzed using field testing techniques. RESULTS : The results were as follows: 1) the results of the luminance performance standards tests showed that all of the phosphorescence test specimens satisfied the phosphorescent fire protection standard. As the phosphorescent pigment mixing ratio increased, the luminance value increased, 2) the luminance reduction rate was minimum at the mixing ratio of 50%. However, when compared to a mixing ratio 40%, a small difference was recorded, the luminance reduction rate from the mixing ratio of 40% is judged as being converged. Therefore, in view of the economic efficiency, it was determined that the optimal mixing ratio was 40%, 3) as a result of construction on the field, a mixing ratio of 40% was found to have a higher luminance value than the general line marking for up to three hours after sunset, 4) it was found that the phosphorescent line markings without glass beads spraying had a higher luminance value than the phosphorescent line markings with glass beads spraying. CONCLUSIONS : Through the results of the basic experiments of the line markings obtained by blending a phosphorescent pigment, the results could be applied to play an important role in the development of phosphorescent line marking paint technology and in establishing application planning for on-site construction characteristics.

• Korean Journal of Soil Science and Fertilizer
• /
• v.34 no.5
• /
• pp.333-341
• /
• 2001

The Compost-decomposing-bacteria was isolated from livestock compost containing sawdust. The isolated bacteria was identified as Bacillus subtilis LYH201 by the method of the composition of the fatty acid with MIDI system and Bergey's manual. Cloning of CMCase encoding gene was accompanied by shotgun method. The pLK100 have yellow activity ring on CMC medium, that was carried 2.2 kb insert DNA in pBluescript II $SK^+$ vector, named BglC gene. The BglC was very similar to Pectobacterium carotovorum Gun_CLOAB(P15704) with score of 57% identity and 71% homology over 508 aa. The BglC was measured molecular weight 56 kDa by CMC-SDS-PAGE. Optimum cellulase activity Bacillus subtilis LYH201 was temperature $50^{\circ}C$ and pH 7.

• Korean Journal of Community Nutrition
• /
• v.18 no.2
• /
• pp.177-186
• /
• 2013

The purpose of this study was to investigate factors associated with Bone Mineral Density (BMD) in Korean postmenopausal women. The data from 2008-2010 Korean National Health and Nutrition Examination Survey (KNHANES) were used for data analysis. Subjects were 2,701 postmenopausal women aged ${\geq}50$ years. BMDs at whole body, total femur, femoral neck, and lumbar spine were measured by Dual-energy X-ray absorptiometry (DXA). Dietary data from 24-hour dietary recall and a food frequency questionnaire containing 63 food items were used. The proportions of osteopenia at total femur, femoral neck, and lumbar spine were 37.4%, 54.5%, and 45.4%, respectively. The proportions of osteoporosis at total femur, femoral neck, and lumbar spine were 6.2%, 25.6%, and 34.3%, respectively. Age, anthropometric index including height, weight, and Body Mass Index (BMI), parathyroid hormone, and physical activity were related to BMD, but the relationships were site specific. Total femur BMD was explained by age, weight, parathyroid hormone and intakes of carbohydrate and fruits. Femoral neck BMD was related to age, weight, parathyroid hormone and intakes of riboflavin and fruits. Lumbar spine BMD was associated with age, weight, milk and dairy products, calcium intake, and exercise. These results indicated that adequate intakes of milk and dairy products, fruits, carbohydrate, calcium, riboflavin and exercise as well as weight maintenance might play an important role in maintaining optimum bone health in Korean postmenopausal women.

• The Journal of Engineering Geology
• /
• v.16 no.3 s.49
• /
• pp.265-273
• /
• 2006

Face mapping during tunnel construction is useful and critical to predict the characteristics and stability condition of following tunneling sections and to select optimum support pattern. Therefore, a detailed geological survey of the tunnel faces, as important as a routine underground survey and a RMR evaluation, should provide critical information of the tunnel face condition in terms of the engineering geological condition and the safety of working environment for the following tunneling section. But the results of the face investigation have not been applied satisfactorily during tunneling due to limitation of technique, experience and time. This study analyze problems of face mapping in tunnel construction site by using statistical results of face mapping sheets obtained from completed tunnels, and suggest several opinions to improve face mapping during tunnel construction.

• Applied Chemistry for Engineering
• /
• v.10 no.2
• /
• pp.259-262
• /
• 1999

The copper-catalyzed reaction of silicon with methanol was carried out in a mixed bed reactor to obtain tetramethyl orthosilicate (TMOS). In order to determine the kinetics of the reaction per active site on the silicon surface, a flow rate transition technique was employed. A kinetic study showed the reaction was in Linear relationship with the amount of contact mass and independent on the concentration of methanol. This result indicated that the rate-determining step was not the chemical process involving methanol, but the formation of silicon intermediate on the contact mass. On the basis of optimum experimental conditions, the maximum TMOS formation rate per g-silicon is 0.030 (g/min) at $210^{\circ}C$, in which activation energy was 8.5 kcal/mol and reaction rate equation was $k=4.09{\times}10^4\;exp$ ($-4.73{\times}10^3/T$).

• Journal of the Korean Society for Railway
• /
• v.12 no.5
• /
• pp.788-792
• /
• 2009

Railroad is well known for eco-friendly transportation system. But, for past few decades, there might be happened many contamination acts in railway facility sites. Industrial and municipal solid wastes produced to maintain and fix trains were dumped to underground of railroad depot area. To develop and reconstruct this area, we should remediate the contaminated soil and ground water. This study was conducted to evaluate the soil pollution status of railroad depot and propose the optimum remediation processes. Our investigation showed that main pollutants sources were TPH and some heavy metals from the dump site. The surveying results for the soil under rail track and crossing nose areas showed TPH contamination from crossing nose area causing lubricant agent. It could be use and rehabilitate the railroad facility areas to an intended purpose with an application of well designed in-situ and ex-situ remediation processes.