• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.449-456
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• 2002

The objective of this study was to optimize medium composition of initial pH, tryptone, glucose, yeast extract, and mineral mixture for production of bacteriocin by Lactobacillus acidophilus ATCC 4356, using response surface methodology. A response surface approach including new statistical and plotting methods was employed for design and analysis of the experiment. An interiorly augmented central composite design was used as an experimental design. A normal-distribution log-link generalized linear model based on a subset fourth-order polynomial ($R^2$=0.94, Mean Error Deviance=0.0065) was used as an analysis model. This model was statistically superior to the full second-order polynomial-based generalized linear model ($R^2$=0.80, Mean Error Deviance=0.0140). Nonlinear programming determined the optimum composition of the medium as initial pH 6.35, typtone $1.21\%$, glucose $0.9\%$, yeast extract $0.65\%$, and mineral mixture $1.17\%$. A validation experiment confirmed that the optimized medium was comparable to the MRS medium in bacteriocin production, having the advantage of economy and practicality.

• Geomechanics and Engineering
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• v.19 no.6
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• pp.543-554
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• 2019

İzmir Bay reserves high amount of residual alluvial deposits generated by Meles River at its stream mouth. These carried sediments with high water content and low bearing capacity are unsuitable in terms of engineering purposes. In-situ soil stabilization with deep soil mixing method is considered to improve properties of soil in this location. This method is widely used especially over Scandinavia, Japan and North America. Basically, the method covers mixing appropriate binder into the soil to improve soil profile according to the engineering needs. For this purpose, soil samples were initially provided from the site, classification tests were performed and optimum ratios of lime and cement binders were determined. Following, specimens representing the in-situ soil conditions were prepared and cured to be able to determine their engineering properties. Unconfined compression tests and vane shear tests were applied to evaluate the stabilization performance of binders on samples with different curing periods. Scanning electron microscope was used to observe time-dependent bonding progress of binders in order to validate the results. Utilization of 4% lime and 4% cement mixture for the long-term performance and 8% lime and 8% cement mixture for short term performance were suggested for the stabilization of Meles Delta soils. Development of CSH and CAH in a gel form as well as CSH crystals were clearly observed on SEM images of treated specimens.

• Journal of Ginseng Research
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• v.40 no.2
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• pp.121-126
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• 2016

Background: Ginsenoside F1, a pharmaceutical component of ginseng, is known to have antiaging, antioxidant, anticancer, and keratinocyte protective effects. However, the usage of ginsenoside F1 is restricted owing to the small amount found in Korean ginseng. Methods: To enhance the production of ginsenoside F1 as a 10 g unit with high specificity, yield, and purity, an enzymatic bioconversion method was developed to adopt the commercial enzyme Cellulase KN from Aspergillus niger with food grade, which has ginsenoside-transforming ability. The proposed optimum reaction conditions of Cellulase KN were pH 5.0 and $50^{\circ}C$. Results: Cellulase KN could effectively transform the ginsenosides Re and Rg1 into F1. A scaled-up biotransformation reaction was performed in a 10 L jar fermenter at pH 5.0 and $50^{\circ}C$ for 48 h with protopanaxatriol-type ginsenoside mixture (at a concentration of 10 mg/mL) from ginseng roots. Finally, 13.0 g of F1 was produced from 50 g of protopanaxatriol-type ginsenoside mixture with $91.5{\pm}1.1%$ chromatographic purity. Conclusion: The results suggest that this enzymatic method could be exploited usefully for the preparation of ginsenoside F1 to be used in cosmetic, functional food, and pharmaceutical industries.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.39 no.4
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• pp.14-20
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• 2007

The effects of blending starches with different gelatinization temperatures on the development of ply-bond strength were systematically investigated using a three component mixture design technique. Oxidized corn starches with different gelatinization temperatures were blended with natural corn starch and sprayed for plybonding. Optimum blend ratio for maximizing plybond strength improvement for the starch blends was 40% of natural starch, 27% of oxidized starch with low gelatinization temperature and 33% of oxidized starch with high gelatinization temperature. Starch granules with the lowest gelatinization temperature gelatinizes at the lowest temperature, while the natural corn starch gelatinizes at later stage of drying. The improvement of plybond strength with starch blends were verified on machine trial as well. Plybond strength improvement obtained from the machine trial was lower than that achievable with handsheets, which was attributed to the lower internal bond strength of the linerboards made from recycled fibers.

• Food Science of Animal Resources
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• v.37 no.6
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• pp.813-822
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• 2017

The aim of this study was to prepare diacylglycerol (DAG) by enzymatic glycerolysis of lard. The effects of reaction parameters such as lipase type, reaction temperature, enzyme amount, substrate molar ratio (lard/glycerol), reaction time, and magnetic stirring speed were investigated. Lipozyme RMIM was found to be a more active biocatalyst than Novozym 435, and the optimal reaction conditions were 14:100 (W/W) of enzyme to lard substrate ratio, 1:1 of lard to glycerol molar ratio, and 500 rpm magnetic stirring speed. The reaction mixture was first incubated at $65^{\circ}C$ for 2 h and then transferred to $45^{\circ}C$ for 8 h. At the optimum reaction conditions, the conversion rate of triacylglycerol (TAG) and the content of DAG in the reaction mixture reached 76.26% and 61.76%, respectively, and the DAG content in purified glycerolized lard was 82.03% by molecular distillation. The distribution of fatty acids and Fourier transform infrared spectra in glycerolized lard samples were similar to those in lard samples. The results revealed that enzymatic glycerolysis and molecular distillation can be used to prepare more highly purified DAG from lard.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.25 no.6
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• pp.426-430
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• 2012

The properties of green sheet were investigated in order to understanding an effects of organic solvent mixture ratio for solid oxide fuel cells fabrication. The purpose of this work is to optimize the slurry condition using the design of experiment to improve green sheet properties. The elongation increased with increasing amount of binder and solvent. With increasing amount of solvent, the air permeability increased but the tensile strength decreased. The best properties of the green sheet appeared amount of the binder 17 wt%, solvent 35 wt% and powder 48 wt%. The optimum condition of green and sintered density for solid oxide fuel cells fabrication was obtained in the sample pressured at 800 $kgf/cm^2$.

• International Journal of Highway Engineering
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• v.19 no.2
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• pp.137-142
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• 2017

PURPOSES :The objective of this study is to evaluate the performance of asphalt mixtures containing inorganic additive and a high content of reclaimed asphalt pavement (RAP). METHODS : The laboratory tests verified the superior laboratory performance of inorganic additive compared to cement, in cold recycled asphalt mixtures. To investigate the moisture susceptibility of the specimens, tensile strength ratio (TSR) tests were performed. In addition, dynamic modulus test was conducted to evaluate the performance of cold recycled asphalt mixture. RESULTS :It was determined that NaOH solution mixed with $Na_2SiO_3$ in the ratio 75:10 provides optimum performance. Compared to Type B and C counterparts, Type A mixtures consisting of an inorganic additive performed better in the Indirect tensile strength test, tensile strength ratio test, and dynamic modulus test. CONCLUSIONS : The use of inorganic additive enhances the indirect strength and dynamic modulus performance of the asphalt mixture. However, additional experiments are to be conducted to improve the reliability of the result with respect to the effect of inorganic additive.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.10 s.175
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• pp.39-48
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• 2005

As the industrial development is accelerated, a new machining process and system are keenly required to achieve super precision surface finish. Especially to get ground surface finish fer complicated and narrow inner shape of molds, it is impossible with the existing methods so that a new method is being required to be developed. A new material, called Magic(MAGnetic Intelligent Compounds), is finally made and it is called Magic machining that uses this material. There is a way to make a material as follows, the mixture of magnetic particles, bonding material and particles of abrasive grain should be melt down by proper heat, and then this mixture put in a mold and cool down in magnetic field which has a uniform direction. This new polishing method is spotlighted as an excellent solution to the existing problems. However it hasn't reported any study about the influence of the machining conditions of polishing velocity, amplitude and polishing pressure to the surface roughness yet. This study would examine closely the influence of polishing conditions of the Magic polishing tool to the surface finish to decide the optimum polishing condition and to standardize the Magic polishing work.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.1
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• pp.22-27
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• 2005

To find out the optimum mixture ratio of ammonium and nitrate on rice plant, 4 rice varieties were examined during 14days after transplanting in hydroponics with the different ratio of ammonium to nitrate(100 : 0, 75: 25,50: 50, 25: 75 and 0: 100). The highest N uptake from solution and the maximum plant dry weight were $60\~70\%$ ammonium and $30\~40\%$ nitrate mixture treatment both in Japonica and Tongil type rice plants. And with the same varieties N-uptake and N use-efficiency were compared between 10.0 mM and 1.0 mM nitrogen using $70\%$ ammonium and $30\%$ nitrate for 24 days after transplanting. Rice plants absorbed more nitrogen$(131\~145\%)$ in 10.0mM than 1.0mM treatment but accumulated N in rice plants were almost the same in both treatment. Among the tested rice cultivars, dry matter production and total accumulative nitrogen in rice plants were much high in Tongil type than japonica type rice cultivars. N-recovery ratios of rice plants from uptake N were $90.8-99.0\%$ in low concentration N solution(1.0 mM), but $69.4-81.7\%$ were observed in high concentration N solution(10.0 mM). It means that suppling low concentration N steadily will be better to prevent loss of N without reducing of growth in rice plants.

• Journal of the Korean institute of surface engineering
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• v.36 no.3
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• pp.277-283
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• 2003

Electrochemical techniques were used to study the surface treatment for improving the pitting corrosion resistance of 304L stainless steel by inhibitors in chloride medium. Sodium molybdate (in concentration range : 0.005-80 g/l) , sodium nitrite (in concentration range : 0.001-50 g/l) and their mixture were used for this study. It was found that, molybdate and nitrite were good passivators for 304L stainless steel, but molybdate was not able to prohibit the pitting ; nitrite prevented pitting corrosion of 304L stainless steel only at the concentration more than 25 g/l. The relationship between pitting potentials and concentrations of inhibitors in the logarithm expression obeyed the linear function. It was found that the surface treatment by mixture of two inhibitors enables stainless steel to have increased the corrosion resistance , the pitting corrosion of 304L stainless steel was completely prohibited by the mixtures of molybdate and nitrite in ratio min, with $m\;\geq\;3\;and\;n\;\geq\;10$. The interesting cases on electrochemical measurement of threshold of inhibitors concentration combination for optimum surface treatment were described.