• 제목/요약/키워드: optimum growth condition

검색결과 605건 처리시간 0.033초

장류용 강력국균 연구 2 (Studies on Koji for Optimum Conditions of Growth and Idenfication of Aspergillus spp.(Part2))

  • 이계호;장건형
    • 미생물학회지
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    • 제2권1호
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    • pp.17-18
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    • 1964
  • Ke Ho Lee and Kun Hyung Chang (Army Research and Testing Lab.): Studies on koji for optimum condition of growth and identification of Aspergillus spp. Cpart 2). Kor. J. Microbiol. Vol. 2, No. 1, p. 17-18 (1964). The experiments reported in this paper are concerned with the identifications, the growth conditions and lethal temperatures of the three strains of Aspergillus spp. which have been isolated from the soils and Meju (fermented soy bean in Korea). The results obtained in the light of the manual of Raper and Thom for identification of the Genus, Aspergillus, have been shown that the three strains of Aspergillus spp. are pertained to Aspergillus flavusoryzae group. The optimum temperature, pH and the lethal temperatures of the Aspergillus spp. have been measured.

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국내 자생 그늘쑥 (Artemisia sylvatica MAX) 모상근의 대량배양을 위한 최적조건 (Optimum Condition for Mass Culture of Hairy Roots from Artemisia sylvatica MAX)

  • 신선희;양덕조
    • Journal of Plant Biotechnology
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    • 제30권1호
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    • pp.65-71
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    • 2003
  • 본 실험은 그늘쑥을 Agrobacterium을 통해 형질전환체인 모상근을 유기하고 모상근의 대량배양을 위해 모상근이 가장 잘 자라는 생장 조건을 찾기 위해 수행되었다. 실험 결과를 종합해 보면 선발된 그늘쑥 모상근 세포주의 대량 생산을 위한 최적 배지는 MS 기본 배지임을 확인하였다. 또한 최적의 havesting time 모상근의 성장률이 좋고 갈변과 배지의 미생물 오염도가 적은 4주가 적당한 것으로 판단된다. 그리고 광 처리 하에서 모상근을 배양하는 것보다는 암상태에서 모상근을 키우고 pH는 5.8 정도가 탄소원은 sucrose로 3.5%가 모상근 을 대량배양시 최적조건임을 확인되었다. 미량원소인 Fe$^{2+}$은 0.1 mM을 처리하는 것이 모상근의 성장을 촉진하였으며 spermine과 spermidine은 각각 10 mM와 100 mM로 처리하는 것이 모상근의 성장을 높일 수 있다. 앞으로 연구는 이 결과를 토대로 모상근 대량 배양을 위한 scale up조건과 bioreator의 설계에 관한 연구가 필요하다.

수소 발효에 의한 폐수처리 및 바이오가스 생산(I): 최적 수소 생산 조건 (Wastewater Treatment and Biogas Production by Hydrogen Fermentation(I): Optimum Condition for Hydrogen Production)

  • 선용호;한정우박돈희조영일
    • KSBB Journal
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    • 제6권4호
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    • pp.351-361
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    • 1991
  • This study is on the investigation of hydrogen production and substrate removal by photosynthetic bacteria. After using of Rhodospillum rubrum KS-301 and IFO 3986, which are photosynthetic bacteria as strains, R. rubrum KS-301 was turned out a better strain. And result of experiment in which glucose and sodium lactate, components of wastewater, were used limiting substrates, showed that the productivity of hydrogen was indifferent with the kind of substrates. In batch experiments using free cells and immobilized whole cells, the decrease in hydrogen productivity was observed in the latter case. From the results of these experiments, specific growth rate of cells, specific utilization rate of glucose, and specific production rate of hydrogen were calculated. And each rate was expressed in the form of Monod equation of which parameters were estimated. Also the optimum condition of hydrogen production for free cells was $30^{\circ}C$, pH 7, and 12,000 Lux, and the optimum immobilized condition was as follows: initial immobilized cell concentration 1.0g/L, sodium alginate concentration 2% and light intensity 12,000 Lux.

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광합성 세균의 분리 동정 및 두부 유청 폐수에서의 배양조건 검토 (Isolation of Photosynthetic Bacteria and the Cell Growth on Soybean-Curd Whey Wastes)

  • 지근억;이계호
    • 한국식품과학회지
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    • 제25권2호
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    • pp.142-147
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    • 1993
  • 일광 에너지를 고정하고 식품공장의 폐수를 이용할 목적으로 56점의 분리원으로부터 광합성 세균 179주를 분리하고 그중 두부유청에서 제일 잘 자라는 균주를 선정하여 동정하고 두부유청을 기질로 하여 배양 최적조건을 조사하였다. 선정균주 B-Ps-106은 Rhodopseudomonas sphaeroides의 변종 또는 근연균으로 동정되었고 호기 배양보다 광혐기 배양에서 더 양호한 성장을 나타냈다. 최적 pH는 $8.5{\sim}9.0$이었고 최적온도는 $30^{\circ}C$ 이었고 최적광량은 $0.72\;ca1/cm^2/min$ 이상이었다. $K_2HPO_4$$(NH_4)_2SO_4$ 농도를 달리하여 각각 첨가한 결과 양쪽에서 모두 0.9 g/l 첨가했을 때 비교적 양호한 성장을 나타냈다.

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기수산 물벼룩, Diaphanosoma celebensis의 대량배양을 위한 최적 염분 및 수온 조건 (Optimum Salinity and Temperature Condition for Mass Culture of the Brackish Water Flea, Diaphanosoma celebensis)

  • 박진철;박흠기
    • 한국수산과학회지
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    • 제43권2호
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    • pp.139-145
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    • 2010
  • This study investigated the optimum salinity and temperature conditions for mass culture of the brackish water flea, Diaphanosoma celebensis. Community and individual cultures of flea were maintained in 1 L beakers and 3 mL vessels (of a 12-well culture plate), respectively, and fed green algae, Tetraselmis suecica. In salinity experiments ranging from 5 to 34 psu, continuous growth of flea populations was found up to 34 psu. However, the specific growth rate and life span of females showed decreasing tendencies with the increase of salinity. The highest maximum density and offspring number were 33.6 individuals (ind.)/mL and 55.3 ind. at 10 psu, respectively. In the temperature experiments ranging from 20 to $40^{\circ}C$, population growth of D. celebensis increased continuously until $35^{\circ}C$ and then decreased over $40^{\circ}C$. The specific growth rate was significantly higher at 25 and $30^{\circ}C$ than at 20 and $40^{\circ}C$. Female life span tended to decrease with temperature increase. The highest maximum density and offspring number were 52.3 ind./mL and 46.0 ind. at $30^{\circ}C$, respectively. These results suggest that the optimum salinity and temperature for mass culture of D. celebensis may be 10 psu and $30^{\circ}C$, respectively.

Growth and fatty acid composition of three heterotrophic Chlorella species

  • Kim, Dae Geun;Hur, Sung Bum
    • ALGAE
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    • 제28권1호
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    • pp.101-109
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    • 2013
  • Some Chlorella species grow heterotrophically with organic substrate in dark condition. However, heterotrophic Chlorella species are limited and their optimum culture conditions are not fully known. In this study, three heterotrophic Chlorella species, two strains (C4-3 and C4-4) of C. vulgaris and one Chlorella sp. (C4-8) were examined on optimum culture conditions such as carbon source, temperature, and concentrations of nitrogen and phosphorus in Jaworski's medium (JM). And the growth and fatty acid composition of Chlorella were analyzed. For three heterotrophic Chlorella species, glucose (1-2%) as a carbon source only increased the growth and the range of optimum culture temperature was $26-28^{\circ}C$. Doubled concentrations of the nitrogen or phosphorus in JM medium also improved the growth of Chlorella. Chlorella cultured heterotrophically showed significantly higher growth rate and bigger cell size than those autotrophically did. C. vulgaris (C4-3) cultured heterotrophically showed the highest biomass in dry weight ($0.8g\;L^{-1}$) among three species. With respect to fatty acid composition, the contents of C16:0 and n-3 highly unsaturated fatty acid (HUFA) were significantly higher in autotrophic Chlorella than in heterotrophic one and those of total lipid were not different between different concentrations of nitrogen and phosphorus in JM medium. Among three Chlorella species in this study, C. vulgaris (C4-3) appeared to be the most ideal heterotrophic Chlorella species for industrial application since it had a high biomass and lipid content.

광합성 홍색세균에 의한 5-Aminolevulinic acid생산에서의 조도의 영향

  • 민나영;윤종선;위영중;김진남;류화원
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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    • pp.295-296
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    • 2000
  • Effect of light intensity on ALA production was, investigated. The culture condition and medium optimization were also examined for the biosynthesis of ALA using Rhodobacter sphaeroides, non-sulfur bacteria, and investigated for enhancement of the production of ALA. In the dark condition, extracellular ALA formation and cell growth were not observed. Optimum light intensity for cell growth and ALA production were 4 kLux and 5 kLux, respectively.

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Growth of Spirulina platensis in Effluents from Wastewater Treatment Plant of Pig Farm

  • Hong, Seok-San
    • Journal of Microbiology and Biotechnology
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    • 제3권1호
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    • pp.19-23
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    • 1993
  • Spirulina platensis NIES 39 was grown in effluents from a wastewater treatment plant utilizing activated sludge process at a pig farm to reduce pollutants and to produce feed resources. The addition of 2 g/l NaCl was required for the growth of S. platensis in the effluents with about 100 mg/l ammonia nitrogen concentration. The growth was stimulated by the addition of 2 g/l sodium bicarbonate and 4 mg/l ferrous sulfate. The microorganism grew well at pH 8~11, and the optimum pH for the growth was 10. The algal concentration of 1.1 g/l was attained after 8 days of growth at the optimum condition with the removal of 95% ammonia nitrogen and 34% phosphorus.

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생태방음벽에 개발에 사용되는 식물종의 성장에 관한 연구 (Development of Ecological Sound Proof Wall by the germination of plant species at different Environmental Condition)

  • 사리타 버셀;조해용;한세권
    • 한국산학기술학회:학술대회논문집
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    • 한국산학기술학회 2009년도 추계학술발표논문집
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    • pp.100-102
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    • 2009
  • Effect of temperature, pH and soil depth on germination of Dianthus chinensis, Dianthus barbatus, and Perennial pennant were investigated in growth chamber and soil condition at the ratio of 7:3 (natural soil and organic soil) in laboratory condition. the optimum temperature for seed germination was recorded for $20^{\circ}C-\;25^{\circ}C$. Maximum germination was observed for Dianthus barbatus (76%) where as in soil condition Perennial pennant (51%) showed maximum germination at 1 cm soil depth. Similarly, optimum pH for seed germination was at pH 6 in all the species. So in lower pH (at pH4) seed germination was inhibited. Germination of these selected species at different environmental condition help to construct the ecological sound proof wall to mitigate the noise especially in urban areas.

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유기산의 저해를 조절하기 위한 Escherichia coli의 전기투석배양 (Control of Organic Acid Inhibition in Escherichia coli Culture with Eledtroudialysis)

  • 김인호;윤태호
    • 한국미생물·생명공학회지
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    • 제23권6호
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    • pp.747-754
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    • 1995
  • E. coli growth is inhibited by organic acids produced in the broth. In order to reduce the inhibition, an electrodialysis unit was used. Model solutions (acetic acid plus distilled water or M-9 medium) were tested in the unit for investigating the optimum condition of current and voltage. Electrodialysis cultures were performed with the optimum condition where the highest current efficiency could be attained. The distilled water plus acetic acid gave us a higher current efficiency than the M-9 plus acetic acid. Electrodialysis efficiently removed acetic acid and so enhanced the specific growth rate of E. coli compared with the control experiment without clectrodialysis.

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