• KDI Journal of Economic Policy
• /
• v.12 no.3
• /
• pp.125-151
• /
• 1990

This paper reviews the economic liberalization experiences of the Southern Cone countries and draws some lessons from their experiences. The Southern Cone countries-Chile, Argentina and Uruguay-followed the different sequences in liberalization. Chile implemented the fiscal reform and the following comprehensive trade reform in the beginning of liberalization, but capital controls were maintained until 1979. Argentina and Uruguay placed more emphasis on the financial reform with the goods market reformed afterwards, but the fiscal sector was never reformed in Argentina. Since the serious inflation plagued the Southern Cone countries, they combined the economic liberalization scheme with the economic stabilization programmes which are based on the monetarist model. Although economic situations in the Southern Cone countries are quite different from those of Korea, we can learn many lessons from their experiences. First, the monetary and fiscal policies should consist of strict financial discipline to bring in the stable domestic inflation. Without the domestic stabilization, the financial liberalization could disturb the domestic economy as the capital inflows in particular generate a real exchange rate appreciation. Second, the monetary approach which is based on the full purchasing power parity and perfect capital mobility make stabilization as simple as a matter of the appropriate exchange rate policy and the proper rate of domestic credit creation. The unsuccessful experiences with monetarist stabilization in the Southern Cone countries suggest that the monetarist model cannot make real exchange rate and real interest rate stable with the trade and financial reform. Third, both the theory and practice have not yet provided a precise solution on the optimal sequencing and speed of the goods and financial market. Nonetheless, it seems desirable to keep the real exchange rate and the real interest rate stable by gradually opening up the current account and then the capital account.

• Korean Journal of Environmental Biology
• /
• v.30 no.1
• /
• pp.31-38
• /
• 2012

Recently, energy security is one of the most important world-wide issues. Biodiesel derived from microalgae has received much attention as a renewable bioenergy. The green colonial alga, $Botryococcus$ $braunii$, is characterized by the ability to produce and accumulate large amounts of hydrocarbons and fatty acids. In this study, we have isolated 5 strains of $B.$ $braunii$ from Korean surface waters using a microcapillary-pipetting method and identified them by morphological features and phylogenetic analysis of the 18S rRNA gene. Phylogenetic analysis indicates that 5 strains of $B.$ $braunii$ are placed in the class of Trebouxiophyceae, and strains belong to race A type producing hydrocarbons which are alkadienes and alkatrienes. In addition, we need further studies to find out optimal growth conditions for producing biodiesel.

• Journal of Life Science
• /
• v.26 no.5
• /
• pp.571-583
• /
• 2016

Biogenic amines are produced primarily by microorganisms found in fermented foods and are often implicated in poisoning incidents in humans. In this study, 620 strains of microorganisms were isolated from traditional Korean fermented food in Sunchang in order to screen for non-biogenicamine-producing microorganisms present in these foods. One strain was identified and named Bacillus subtilis SCJ1, by using 16S rRNA sequencing and biochemical characterization. We investigated the cell growth of this organism in order to understand its potential for industrial application. To this end, we optimized the culture medium constituents by using the response surface methodology. The Plackett-Burman experimental design was used for screening of the medium constituents, such as molasses, yeast extract and peptone, for improving cell growth. In order to determine the optimal concentration of each constituent, we used a central composite design. Consequently, the optimized concentrations of molasses, yeast extract and peptone were predicted to be 27.5 g/l, 7.5 g/l and 17.5 g/l, respectively. By model verification, we confirmed that a 1.49-fold increase in dry cell weight compared to the basal medium-from 1.32 g/l, to 1.9722 g/l-was achieved.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.11 no.6
• /
• pp.2021-2029
• /
• 2010

This paper suggested the method to design and analyze FMC robot's dispatching rule using the Simulation and Sequential Patterns. To do this, first of all, we built FMC using simulation and then, extracted signals that facilities call a robot, saved it as the log type. Secondly, we built robot's optimal path using the Sequential Pattern Mining with the results of analyzing the log and relationship between machine and robot actions. Lastly, we adapted it to the A corp.'s manufacturing line for verifying its performance. As a result of applying the new dispatching rule in FMC, total throughput and total flow time decrease because of decreasing material loss time and increasing robot utility. Furthermore, because this method can be applied for every manufacturing plant using simulation, it can contribute to advance total FMC efficiency as well.

• Korean Journal of Microbiology
• /
• v.51 no.1
• /
• pp.48-60
• /
• 2015

612 strains isolated from Korean traditional fermented food in Sunchang and their investigated biochemical characterization and ability of biogenic amines non-producing. We selected the SCJ4 having various activity by measurement of extracellular enzyme, antioxidant and antimicrobial activities. Selected strain SCJ4 by 16S rRNA sequencing and biochemical characterization was named Bacillus subtilis SCJ4. And then, we investigated cell growth of SCJ4, and optimized of culture medium constituents using response surface methodology as statistically method. Response surface methodology used Plackett-Burman experimental design for screening of medium constituent. Tryptone, peptone and $MgSO_4$ as medium constituent improving cell growth selected. In order to find out optimal concentration on each constituent, we carried out central composite design. Consequently, optimized concentrations of tryptone, peptone and $MgSO_4$ were predicted to be 15.35 g/L, 12.235 g/L, and 3.5 g/L respectively. Through the model verification, we confirmed about 1.28-fold improvement of the dried cell weight from 0.8767 g/L to 1.1222 g/L when compared to basal medium.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.42 no.3
• /
• pp.226-232
• /
• 2015

Critical pathway (CP) defines the optimal care process, sequencing and timing of intervention by multidisciplinary health care teams for a particular diagnosis and procedure. The aim of the study was to evaluate the clinical usefulness and the satisfaction of patients and dental staff after implementation of a critical pathway for the dental treatment of disabled children and adolescents under conscious sedation. Thirty patients are divided in two groups (Pre-CP and CP) at the department of Pediatric Dentistry of Pusan National University Dental Hospital. The satisfaction levels of patients/guardians and the staff members were collected by survey questionnaire. The parents' satisfaction was significantly improved after the implementation of CP. Also, medical/dental staff members were highly satisfied with its usefulness. The application of a critical pathway for disabled children and adolescents might be useful and improve the satisfaction of the parents and medical/dental staff members.

• Korean Journal of Microbiology
• /
• v.38 no.2
• /
• pp.74-80
• /
• 2002

The purpose of this work was to investigate the characterization and sequence of catechol 1,2-dioxygenase (Cl,2O) purified from Acinetobacter sp. KS-1 which was grown on benzoate as a sole carbon source. Cl,2O demonstrated its enzyme activity to catechol and 4-methylcatechol. The optimum temperature of Cl,2O was $35^{\circ}C$, and the optimal pH was in the range from pH 7.5 to 9.0. $Ag^{+}$, $Hg^{+}$, and $Cu^{2+}$ showed inhibitory effect on the activity of Cl,2O. Molecular weight of the enzyme was determined to approximately 36 kDa by SDS-PAGE and 7-terminal amino acid sequence of Cl,2O was analyzed as $^{1}MNYQQIDALVKQMNVDTAKG^{20}$and exhibited 95% sequence homology with that of Cl,2O from Acinetobacter radioresistens In addition, trypsin digestion and peptide mapping were performed for internal sequencing analysis. Molecular weights of three digested peptide fragments were analyzed as 966.3 Da, 1933.8 Da and 2081.7 Da by MALDI-TOF, which were matched with each internal sequences $^{1}SQSDFNLRR^{9}\, ^{1}HGNRPSHVHYFNSAPGYR^{18}\, ^{1}TIEGPLYVAGAPESVGFAR^{19}$) of. A. radioresistens. PCR product was amplified with the degenerated primers derived from N-terminal and each internal amino acid sequences.

• Journal of Life Science
• /
• v.16 no.4
• /
• pp.555-560
• /
• 2006

A bacterium producing novel lipolytic enzyme was isolated from house sewage and identified as Acinetobacter sp. BD5 based on physiological characterization and 16S rDNA sequencing. The lipolytic activity of Acinetobacter sp. BD5 was tested using an EL agar medium and CE agar medium supplemented with 1% tributyrin and olive oil, respectively. The formation of a clear zone around the colony was detected by agar medium supplemented with 1% tributyrin and olive oil, respectively and Acinetobacter sp. BD5 formed powder-like zone around the colony on LB agar medium containing Tween 20. The quantitative lipolytic activity was determined by using p-NP butyrate as substrate. Acinetobacter sp. BD5 secreted the lipolytic enzyme during exponential growth phase, reaching a maximum amount after 6 hours of incubation. The lipolytic enzyme was found to be optimally active at $60^{\circ}C$ and retained more than 70% at $70-80^{\circ}C$. It displayed a high degree of activity in a pH of 7.0 to 10.6, with an optimal pH of 9.0.

• Journal of Life Science
• /
• v.16 no.7 s.80
• /
• pp.1158-1163
• /
• 2006

Bisphenol A (BPA), 2,2-bis(4-hydroxyphenyl) propane, has been widely used as a monomer for production of epoxy resins and polycarbonate plastics, and final products of BPA include adhesives, protective coatings, paints, optical lens, building materials, compact disks and other electrical parts. Since BPA is a toxic chemical to elicit acute cell cytotoxicity and chronic endocrine disrupting activity, the degradation of BPA has been focused during last decades. To overcome the problem of photo-, and chemical-degradation of BPA, in this study, a bacterium that is able to biodegrade BPA, was isolated. The bacterium, isolated froln the soil of plastic factory, was identified as Acinetobacter calcoaceticus (strain BP-2) based on physiological and 16S rDNA sequencing analysis. A. calcoaceticus BP-2 was able to grow in the presence of $1140{\mu}g\;ml^{-1}$ BPA. Biodegradation experiments showed that BP-2 mineralized BPA via 4-hydroxybenzoic acid and 4-hydroxyacetophenone, and average degradation rate was $53.3{\mu}g\;ml^{-1}\;day^{-1}$ under optimal conditions (pH 7 and $30^{\circ}C$). In high density resting cell $(3.5g-dcw.1^{-1})$ experiments, the maximal degradation rate was increased to $89.7{\mu}g\;ml^{-1}\;h^{-1}$. Our results suggest that BP-2 has high potential as a catalyst for practical BPA bioremediation.

• Journal of Life Science
• /
• v.23 no.2
• /
• pp.259-266
• /
• 2013

To isolate the fibrinolytic enzyme, 268 strains from 21 samples were morphologically isolated from Cheonggukjang collected from Korea and Japan. Among the 268 strains, protease-producing bacteria were isolated in nutrient agar medium including 1% skimmed milk. As a result of this, 22 strains were isolated. Apiweb site was used to identify these strains based on their biochemical properties. In addition, 16S rRNA sequencing was performed to identify the strain. Most of the identified strains were Bacillus subtilis and B. amyloliquefaciens. Fibrinolytic enzyme activity was measured with the fibrin plate method. Five strains were finally selected: A2-14, A2-20, C1-05, C1-09, and F2-01. Of those five strains, the A2-20 strain, which is close to B. amyloliquefaciens, showed the strongest fibrinolytic activity. The fibrinolytic enzyme produced by the A2-20 strain was partially purified from culture supernatant by gel filtration and ion exchange chromatography. The optimal pH and temperature values of the partially purified enzyme were 7.0 and $35^{\circ}C$, respectively. Purified protein analysis was carried out with SDS-PAGE and zymography. A genetic analysis was also conducted by PCR based on the consensus sequence of fibrinolytic enzyme. Corresponding genes with a partial sequence of the A2-20 strain were identified.