Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Isolation and Phylogenetic Analysis of $Botryococcus$ $braunii$ (Trebouxiophyceae) from Korean Freshwaters

한국산 $Botryococcus$ $braunii$ (Trebouxiophyceae)의 분리 및 계통분류학적 분석

  • Lee, Seung-Hoon (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Chan-Hee (Kyungpook National University (KNU)) ;
  • Jo, Beom-Ho (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ahn, Chi-Yong (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Han-Soon (Kyungpook National University (KNU)) ;
  • Oh, Hee-Mock (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 이승훈 (한국생명공학연구원 환경바이오연구센터) ;
  • 이찬희 (경북대학교 생물학과) ;
  • 조범호 (한국생명공학연구원 환경바이오연구센터) ;
  • 안치용 (한국생명공학연구원 환경바이오연구센터) ;
  • 김한순 (경북대학교 생물학과) ;
  • 오희목 (한국생명공학연구원 환경바이오연구센터)
  • Received : 2012.02.05
  • Accepted : 2012.02.24
  • Published : 2012.03.01
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Abstract

Recently, energy security is one of the most important world-wide issues. Biodiesel derived from microalgae has received much attention as a renewable bioenergy. The green colonial alga, $Botryococcus$ $braunii$, is characterized by the ability to produce and accumulate large amounts of hydrocarbons and fatty acids. In this study, we have isolated 5 strains of $B.$ $braunii$ from Korean surface waters using a microcapillary-pipetting method and identified them by morphological features and phylogenetic analysis of the 18S rRNA gene. Phylogenetic analysis indicates that 5 strains of $B.$ $braunii$ are placed in the class of Trebouxiophyceae, and strains belong to race A type producing hydrocarbons which are alkadienes and alkatrienes. In addition, we need further studies to find out optimal growth conditions for producing biodiesel.

한국 내 전국적으로 5곳의 담수에서 2010년 4월부터 2011년 6월에 걸쳐 담수시료를 채취한 후, 각 담수시료에서 $Botryococcus$ $braunii$의 출현을 확인하고 microcapillary-pipetting method를 이용하여 5주를 분리하였다. 분리된 $B.$ $braunii$의 18S rRNA sequencing 후, BLAST search를 통한 분석 결과는 $B.$ $braunii$와 가장 높은 상동성을 나타낸 것으로 확인되어 형태적 동정결과와 일치하였다. 진화, 계통분류학적 분석결과, 분리된 5주의 $B.$ $braunii$는 단일 계통인 Trebouxiophyceae으로 분류되었다. 18S rRNA sequence의 계통 분류학적 분석에서 $B.$ $braunii$ KNU6는 다른 분리된 $B.$ $braunii$와 같은 분류군 내에서 거리의 차이가 있었고, 또한 세포크기, 점액질의 양 등의 형태적 특징도 차이를 보였다. 한국에서 분리된 5주의 $B.$ $braunii$는 모두 race A type의 분류군에 포함되며, 탄소수 $C_{23}-C_{33}$의 지방산에서 유래된 alkadienes과 alkatrienes를 생산하는 것으로 사료되며, $B.$ $braunii$를 이용한 바이오디젤을 생산하기 위한 최적의 배양조건을 찾는 연구가 필요하다.

Keywords

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