• Journal of Microbiology and Biotechnology
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• 제20권3호
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• pp.467-473
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• 2010

To improve the expression efficiency of recombinant endo-$\beta$-1,4-glucanase in P. pastoris, the endo-$\beta$-1,4-glucanase (egI) gene from Aspergillus niger was synthesized using optimized codons. Fourteen pairs of oligonucleotides with 15 bp overlap were designed and the full-length syn-egI gene was generated by two-step PCR-based DNA synthesis. In the synthesized endo-$\beta$-1,4-glucanase gene syn-egI, 193 nucleotides were changed, and the G+C content was decreased from 54% to 44.2%. The syn-egI gene was inserted into pPIC9K and transformed into P. pastoris GS115 by electroporation. The enzyme activity of recombinant P. pastoris stain 2-7# reached 20.3 U/ml with 1% barley $\beta$-glucan and 3.3 U/ml with 1% carboxymethylcellulose (CMC) as substrates in shake flasks versus 1,270.3 U/ml and 220.7 U/ml for the same substrates in 50-1 fermentors. The molecular mass of the recombinant protein was approximately 40 kDa as determined by SDS-PAGE analysis, the optimal temperature for recombinant enzyme activity was $70^{\circ}C$, and the optimal pH was 5.0 when CMC was used as the substrate.

• 한국통신학회논문지
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• 제35권4A호
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• pp.360-370
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• 2010

본 논문에서는 다수의 송수신기 쌍과 다수의 중계 노드로 구성된 분산 빔포밍 Amplify-and-Forward (AF) 중계 네트워크를 다루고 있다. 제안한 방법에서는 빔포머 수의 자유도를 높이기 위하여 첫 번째 단계에서 송신기들이 직교 채널을 이용하여 중계기로 신호를 보낸다. 두 번째 단계에서 각 중계기는 송신기로부터 받은 신호에 복소 가중치를 곱하여 신호를 증폭하고 역방향 채널에 의해 생긴 위상 변화를 조정하여 하나의 채널로 수신기에 신호를 보낸다. 최적의 빔포밍 벡터는 서비스 품질 수준을 만족시키기 위해 수신기 각각의 신호 대 간섭 및 잡음비가 특정 문턱값보다 크면서 전체 중계 전송 전력을 최소화시키도록 구하였다. 모의실험을 통해 기존의 직교 분할 다중화 방법과 공간 분할 다중화 방법에 비하여 적당히 낮은 데이터 속도 범위에서 중계 송신 전력을 줄일 수 있음을 확인하였다.

• 천문학회지
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• 제54권4호
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• pp.113-119
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• 2021

It is difficult for observers to conduct an optical alignment at an observatory without the assistance of an optical engineer if optomechanical parts are to be replaced at night. We present a practical tilt correction method to obtain the optimal optical alignment condition using the symmetricity of optical aberrations of a wide-field on-axis telescope at night. We conducted coarse tilt correction by visually examining the symmetry of two representative star shapes obtained at two guide chips facing each other, such as east-west or north-south pairs. After coarse correction, we observed four sets of small stamp images using four guide cameras located at each cardinal position by changing the focus positions in 10-㎛ increments and passing through the optimum focus position in the range of ±200 ㎛. The standard deviation of each image, as a function of the focus position, was fitted with a second-order polynomial function to derive the optimal focus position at each cardinal edge. We derived the tilt angles from the slopes converted by the distance and the focus position difference between two paired guide chip combinations such as east-west and north-south. We used this method to collimate the on-axis wide-field telescope KMTNet in Chile after replacing two old focus actuators. The total optical alignment time was less than 30 min. Our method is practical and straightforward for maintaining the optical performance of wide-field telescopes such as KMTNet.

• 방송공학회논문지
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• 제28권2호
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• pp.194-212
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• 2023

다수의 색상 및 거리 순서쌍으로 구성된 몰입형 영상 압축을 위한 MPEG immersive video (MIV) 표준은 시점 간 중복 영역 제거 후 잔여 영상을 병합하여 높은 압축률을 확보하였다. 비슷한 영역을 표현하는 시점 간 그룹화를 통해 품질 향상 및 선택적 스트리밍 구현이 가능하나, 최근 그룹 기반 MIV 부호화 기술은 활발히 논의되고 있지 않다. 본 논문은 최신 MIV 참조 소프트웨어에서 그룹 기반 부호화 기술을 이식하고, 최적의 그룹 별 시점 및 영상 개수 산출을 위한 실험을 진행하였으며, 출력 영상 내 잔여 영상의 비율을 기반으로 전역적 영상 표현을 위한 최적의 출력 영상 수를 결정하는 기법을 제안한다.

• Journal of information and communication convergence engineering
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• 제4권4호
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• pp.141-146
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• 2006

In these days, wireless data services are becoming ubiquitous in our daily life because they offers several fundamental benefits including user mobility, rapid installation, flexibility, and scalability. Moreover, the requests for various multimedia services and the Quality of Service (QoS) support have been one of key issues in wireless data communications. Therefore the research relative to Medium Access Control (MAC) has been progressing rapidly. Especially a number of QoS-aware MAC schemes have been introduced to extend the legacy IEEE 802.11 MAC protocol which has not guaranteed any service differentiation. However, none of those schemes fulfill both QoS features and channel efficiency although these support the service differentiation based on priority. Therefore this paper studies a novel MAC scheme, referred to as Enhanced Distributed Coordination Function with Network Adaptation (EDCF-NA), for enhancements of both QoS and medium efficiency. It uses a smart factor denoted by ACK rate and Network Load Threshold (TH). In this paper, we study how the value of TH has effect on MAC performance and how the use of optimal TH pair improves the overall MAC performance in terms of the QoS, channel utilization, collision rate, and fairness. In addition, we evaluate and compare both the performance of EDCF-NA depending on several pairs of TH and the achievement of various MAC protocols through simulations by using Network Simulator-2 (NS-2).

• 대한전자공학회논문지SP
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• 제41권3호
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• pp.1-8
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• 2004

본 논문은 영상에서 등광도선 정보를 사용한 상표 및 자막영역의 원영상 복원 방법을 제안한다. 제안한 방법은 유사한 밝기값을 가진 픽셀들을 연결한 등광도선을 영상복원을 위한 정칙화 연산자로 사용하고, 이 정칙화 연산자를 가진 비용 함수를 유전자 알고리즘을 사용하여 최소화하는 픽셀값을 추정하여 영상을 복원한다. 제안한 방법을 영상에 포함된 상표 및 자막영역과 노이즈를 제거하고 원영상으로 복원하는데 적용한 결과, 정칙화 이론에서 사용하는 Laplacian과 같은 미분 연산자보다 등광도선 연산자가 ISNR의 향상과 복원효과가 뛰어남을 알 수 있다.

• Journal of Electrical Engineering and Technology
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• 제12권2호
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• pp.654-664
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• 2017

This paper proposed an unified dual-phase-shift (UDPS) control for dual active bridge (DAB) converters in order to improve efficiency for a wide output power range. Different operating modes of UDPS are characterized with respect to the reactive current distribution. The proposed UDPS has the same output power capability with conventional phase-shift (CPS) method. Furthermore, its implementation is simple since only the change of the leading phase-shift direction is required for different operating power range. The proposed UDPS control can minimize both the inductor rms current and the circulating reactive current for various voltage conversion ratios and load conditions. The optimal phase-shift pairs for two bridges of DAB converter are derived with respect to the comprehensive reactive power loss model, including the reactive components delivered from the load and back to the source. Simulation and experimental results are illustrated and explained with details. The effectiveness of the proposed method is verified in terms of reactive power losses minimization and efficiency improvement.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제14권4호
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• pp.1648-1672
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• 2020

There are several types of image registration in the sense of stitching separated images that overlap each other. One of these is feature-based registration by a common feature descriptor. In this study, we generate a mosaic of images using feature-based registration for drone aerial images. As a feature descriptor, we apply the scale-invariant feature transform descriptor. In order to investigate the authenticity of the feature points and to have the mapping function, we employ the sample consensus method; we consider the sensed image's inherent characteristic such as the geometric congruence between the feature points of the images to propose a novel hypothesis estimation of the mapping function of the stitching via some optimally chosen initial candidate inliers in the sample consensus method. Based on the experimental results, we show the efficiency of the proposed method compared with benchmark methodologies of random sampling consensus method (RANSAC); the well-ordering property defined in the context and the extensive stitching examples have supported the utility. Moreover, the sample consensus scheme proposed in this study is uncomplicated and robust, and some fatal miss stitching by RANSAC is remarkably reduced in the measure of the pixel difference.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 1999년도 Proceedings of International Symposium on Remote Sensing
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• pp.474-478
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• 1999

SAR interferometry (InSAR) using the space-borne Synthetic Aperture Radar (SAR) have recently become one of the most effective tools monitoring surface changes caused by landslides, earthquakes, subsidences or volcanic eruption. This study focuses on examining the feasibility of InSAR using the RADARSAT data. Although the RABARSAT SAR with its high resolution and variable incidence angle has several advantages for repeat-pass InSAR, it has two key limitations: first, the orbit is not precisely known; and second, RADARSAT's 24-day repeat pass interval is not very favourable for retaining useful coherence. In this study, two pairs of RADARSAT data in the Nahanni area, NWT, Canada have been tested. We will discuss about the special consideration required on the interferometric processing steps specifically for RADARSAT data including image co-registration, spectral filtering in both azimuth and range, estimation of the interferometric baseline, and correction of the interferogram with respect to the "flat earth" phase contribution. Preliminary results can be summarized as: i) the properly designed azimuth filter based upon the antenna characteristic improves coherence considerably if difference in Doppler centroid of the two images is relatively large; ii) the co-registration process combined by fringe spectrum and amplitude cross-correlation techniques results in optimal matching; iii) the baseline is not always possible to be estimated from the definitive orbit information.

• Current Research on Agriculture and Life Sciences
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• 제32권1호
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• pp.43-52
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• 2014

Reactive oxygen and nitrogen species (ROS and RNS, respectively) are messengers that carry signals to alter the redox state in order to activate plant responses and other physiological processes, such as differentiation, aging, senescence, and pathogen defense. Quite a large number of genes are involved in this signaling and lead to oxidative stress in plants. Although the role of ROS/RNS during stress conditions is well documented, a comprehensive list of genes and comparative study of these genes has not yet been completed. Accordingly, the in silico identification of oxidative stress-related genes was performed for soybeans and Arabidopsis. These genes were also studied in relation to multiple domain prediction. The presence of domains like dehydogenase and ATPase suggests that these genes are involved in various metabolic processes, as well as the transportation of ions under optimal environmental conditions. In addition to a sequence analysis, a phylogenetic analysis was also performed to identify orthologous pairs among the soybean and Arabidopsis oxidative stress-related genes based on neighbor joining. This study was also conducted with the objective of further understanding the complex molecular signaling mechanism in plants under various stress conditions.