High-Level Expression of an Aspergillus niger Endo--1,4-Glucanase in Pichia pastoris Through Gene Codon Optimization and Synthesis |
Zhao, Shumiao
(State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University)
Huang, Jun (State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University) Zhang, Changyi (State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University) Deng, Ling (State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University) Hu, Nan (College of Life Science and Pharmacy, Nanjing University of Technology) Liang, Yunxiang (State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Huazhong Agricultural University) |
1 | Bauer, M. W., L. E. Driskill, W. Callen, M. A. Snead, E. J. Mathur, and R. M. Kelly. 1999. An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes -1,4 bonds in mixed-linkage (1→3),(1→4)-TEX>$\beta$-D-glucans and cellulose. J. Bacteriol. 181: 284-290. |
2 | Henrissat, B. 1991. A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280: 309-316. |
3 | Palomer, X., E. Dominguez-Puigjaner, M. Vendrell, and I. Llop-Tous. 2004. Study of the strawberry Cel1 endo-(1,4)-glucanase protein accumulation and characterization of its in vitro activity by heterologous expression in Pichia pastoris. Plant Sci. 167: 509-518. DOI ScienceOn |
4 | Peilong, Y., P. Shi, Y. Wang, Y. Bai, K. Meng, H. Luo, T. Yuan, and B. Yao. 2007. Cloning and overexpression of a Paenibacillus -glucanase in Pichia pastoris: Purification and characterization of the recombinant enzyme. J. Microbiol. Biotechnol. 17: 58-66. |
5 | Rose, S. H. and W. H. van Zyl. 2002. Constitutive expression of the Trichoderma reesei -1,4-xylanase gene (xyn2) and the -1,4-endoglucanase gene (egI) in Aspergillus niger in molasses and defined glucose media. Appl. Microbiol. Biotechnol. 58: 461-468. DOI ScienceOn |
6 | Saloheimo, A., B. Henrissat, A. M. Hoffren, O. Teleman, and M. Penttila. 1994. A novel small endoglucanase gene, egl5, from Trichoderma reesei isolated by expression in yeast. Mol. Microbiol. 13: 219-228. DOI ScienceOn |
7 | Vervoort, E. B., A. Van Ravestein, N. N. van Peij, J. C. Heikoop, P. J. van Hasstert, G. F. Verheijden, and M. H. Linskens. 2000. Optimizing heterologous expression in Dictyostelium: Importance of 5' codon adaptation. Nucleic Acids Res. 28: 2069-2074. DOI |
8 | Dienes, D., A. Egyhazi, and K. Reczey. 2004. Treatment of recycled fiber with Trichoderma cellulases. Ind. Crop. Prod. 20: 11-21. DOI ScienceOn |
9 | Cazemier, A. E., J. C. Verdoes, H. J. M. Op den Camp, J. H. P. Hackstein, and A. J. J. van Ooyen. 1999. A -1,4-endoglucanaseencoding gene from Cellulomonas pachnodae. Appl. Microbiol.Biotechnol. 52: 232-239. DOI ScienceOn |
10 | David, M. H. and L. Jacek. 2002. DNAWorks: An automated method for designing oligonucleotides for PCR-based gene synthesis. Nucleic Acids Res. 30: e43 DOI |
11 | Duan, X. Y., S. Y. Liu, W. C. Zhang, Q. X. Zhang, and P. J. Gao. 2004. Volumetric productivity improvement for endoglucanase of Trichoderma pseudokoingii S-38. J. Appl. Microbiol. 96: 772-776. DOI ScienceOn |
12 | Huang, Y., G. Krauss, S. Cottaz, H. Driguez, and G. Lipps. 2005. A highly acid-stable and thermostable endo--glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus. Biochem. J. 385: 581-588. DOI ScienceOn |
13 | Lynd, L. R., J. Paul, P. J. Weimer, W. H. van Zyl, and I. S. Pretorius. 2002. Microbial cellulose utilization: Fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66: 506-577. DOI ScienceOn |
14 | Nakamura, Y., T. Gojobori, and T. Ikemura. 1999. Codon usage tabulated from the international DNA sequence database; its status 1999. Nucleic Acids Res. 27: 292. DOI |
15 | Hu, S., L. Li, J. Qiao, Y. Guo, L. Cheng, and J. Liu. 2006. Codon optimization, expression, and characterization of an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris. Protein Expr. Purif. 47: 249-257. DOI ScienceOn |
16 | Chang, S. W., G. C. Lee, and J. F. Shaw. 2006. Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase. J. Agric. Food Chem. 3: 815-822. |
17 | Ooi, T., A. Shinmyo, H. Okada, S. Murao, T. Kawaguchi, and M. Arai. 1990. Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (F1-CMCase). Nucleic Acids Res. 18: 5884. DOI ScienceOn |
18 | Sakamoto, S., G. Tamura, K. Ito, T. Ishikawa, K. Iwano, and N. Nishiya. 1995. Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae. Curr. Genet. 27: 435-439. DOI ScienceOn |
19 | Huang, H., P. Yang, H. Luo, H. Tang, N. Shao, T. Yuan, Y. Wang, Y. Bai, and B. Yao. 2008. High-level expression of a truncated 1,3-1,4--D-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentation. Appl. Microbiol. Biotechnol. 78: 95-103. DOI ScienceOn |
20 | Kitamoto, N., M. Go, T. Shibayama, T. Kimura, Y. Kito, K. Ohmiya, and N. Tsukagoshi. 1996. Molecular cloning, purification and characterization of two endo-1,4--glucanases from Aspergillus oryzae KBN616. Appl. Microbiol. Biotechnol. 46: 538-544. DOI |
21 | Ajithkumar, A., R. Andersson, M. Siika-aho, M. Tenkanen, and P. Aman. 2006. Isolation of cellotriosyl blocks from barley -glucan with endo-1,4--glucanase from Trichoderma reesei. Carbohyd. Polym. 64: 233-238. DOI ScienceOn |
22 | Shuyan, L., D. Xinyuan, L. Xuemei, and G. Peiji. 2006. A novel thermophilic endoglucanase from a mesophilic fungus Fusarium oxysporum. Chinese Sci. Bull. 51: 191-197. DOI ScienceOn |
23 | Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428. DOI |
24 | Teng, D., Y. Fan, Y. L.Yang, Z. G. Tian, L. Luo, and J. H. Wang. 2007. Codon optimization of Bacillus licheniformis -1,3-1,4-glucanase gene and its expression in Pichia pastoris. Appl. Microbiol. Biotechnol. 74: 1074-1083. DOI ScienceOn |
25 | Feng, Y., C. J. Duan, H. Pang, X. C. Mo, C. F. Wu, Y. Yu, et al. 2007. Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases. Appl. Microbiol. Biotechnol. 75: 319-328. DOI ScienceOn |
26 | Hale, R. S. and G. Thompson. 1998. Codon optimization of the gene encoding a domain from human type 1 neurofibromin protein results in a threefold improvement in expression level in Escherichia coli. Protein Expr. Purif. 12: 185-188. DOI ScienceOn |
27 | Macauley-Patrick, S., M. L. Fazenda, B. McNeil, and L.M. Harvey. 2005. Heterologous protein production using the Pichia pastoris expression system. Yeast 22: 249-270. DOI ScienceOn |
28 | Nikolay, S. O., J. S. Willem, and A. J. Maarten. 2002. Optimization of the expression of equistatin in Pichia pastoris. Protein Expr. Purif. 24: 18-24. DOI ScienceOn |
29 | Xiong, A. S., Q. H. Yao, and R. H. Peng. 2004. A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences. Nucleic Acids Res. 32: e98. DOI |
30 | Beguin, P. and J. P. Aubert. 1994. The biological degradation of cellulose. FEMS Microbiol. Rev. 13: 25-58. DOI ScienceOn |
31 | Hong, J., T. Hisanori, A. Shunichi, K. Yamamtot, and H. Kumagai. 2001. Cloning of a gene encoding a highly stable endo--1,4-glucanase from Aspergillus niger and its expression in yeast. J. Biosci. Bioeng. 92: 434-439. DOI ScienceOn |
32 | Teather, R. M. and P. J. Wood. 1982. Use of Congo redpolysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 8: 777-780. |
33 | Invitrogen: Pichia Expression Kit: A manual of methods for expression of recombinant proteins in Pichia pastoris. Version M. Invitrogen, San Diego, CA, 2002 |
34 | Sheppard, P. O., F. J. Grant, P. J. Oort, C. A. Sprecher, D. C. Foster, F. S. Hagen, A. U. Upshall, G. L. McKnight, and P. J. OHara. 1994. The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum. Gene 150: 163-167. DOI ScienceOn |
35 | Sinclair, G. and F. Y. M. Choy. 2002. Synonymous codon usage bias and the expression of human glucocerebrosidase in the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 26: 96-105. DOI ScienceOn |
36 | Bhat, M. K. 2004. Cellulases and related enzymes in biotechnology. Biotechnol. Adv. 18: 355-383. |