• 전기학회논문지
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• 제61권6호
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• pp.879-884
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• 2012

Photoacoustic Tomography (PAT) is a promising medical imaging modality by reason of its particularity. It combines optical imaging contrast of optical imaging with the spatial resolution of ultrasound imaging and can demonstrate change of biological feature in an image. For that reason, many studies are in progress to apply this technic for diagnosis. But, real-time PAT system is necessary to confirm a biological reaction induced by external stimulation immediately. Thus, we developed a real-time PAT system using linear array transducer and self-developed Data acquisition board (DAQ) resources, To evaluate the feasibility and performance of our proposed system, two type of phantom test were also performed. As a result of those experiments, the proposed system shows enough performance and confirm its usefulness.

• 한국전기전자재료학회논문지
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• 제20권8호
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• pp.736-741
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• 2007

Low level laser therapy has various therapy effects, This paper performed the basic study for fabricating the low level laser therapy apparatus, and one of the goals of this paper was to make this apparatus used handily. The apparatus has been fabricated using the laser diode and microprocessor unit. The apparatus used a 660nm, 785nm and 850nm laser diode for laser medical therapy and was designed for a pulse width modulation type to increase stimulation effects. To raise the stimulus effect of the human body, the optical irradiation frequency could be set up. In result, the $1{\sim}10\;Hz$ irradiation frequency could be controlled stably.

• 한국광학회:학술대회논문집
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• 한국광학회 2008년도 동계학술발표회 논문집
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• pp.455-456
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• 2008

근적외선 분광법(Near-infrared spectroscopy, NIRS)은 대뇌피질에서의 혈류변화(oxy-, deoxyhemoglobin의 농도변화)를 비침습적으로 측정할 수 있는 방법이다. 본 논문에서는 향후 뇌-컴퓨터 접속기술(Brain computer interface)에 적용하기위한 초기 연구단계로, 쥐의 수염을 자극시 활성화되는 대뇌수염피질 영역에서의 혈류변화 및 전기신호를 동시에 측정하고 두 신호의 패턴을 분석한다.

• The Journal of Korean Physical Therapy
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• 제21권3호
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• pp.87-93
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• 2009

Purpose: TThis study examined the effect of repetitive magnetic stimulation (RMS) on the viability and proliferative response of human adipose tissue-derived stromal cells (hATSCs) in vitro. Methods: The hATSCs were cultured primarily from human adipose tissue harvested by liposuction and incubated in a $37^{\circ}C$ plastic chamber. The cells were exposed to a repetitive magnetic field using a customized magnetic stimulator (Biocon-5000, Mcube Technology). The RMS parameters were set as follows: repetition rate=10Hz, 25Hz (stimulus intensity 100%= 0.1 Tesla, at 4cm from the coil), stimulated time= 1, 5, and 20 minutes. Twenty four hours after one application of RMS, the hATSCs were compared with the sham stimulation, which were kept under the same conditions without the application of RMS. The cells were observed by optical microscopy to determine the morphology and assessed by trypan blue staining for cell proliferation. The apoptosis and viability of the hATSCs were also analyzed by fluorescence-activated cell sorting (FACS) analysis of Annexin V and MTT assay. Results: After RMS, the morphology of the hATSCs was not changed and the apoptosis of hATSCs were not increased compared to the sham stimulation. The viability of the cells was similar to the cells given the sham stimulation. Interestingly, the level of hATSC proliferation was significantly higher in all RMS groups. Conclusion: The application of RMS may not cause a change in morphology and viability of hATSCs but can increase the level of cell proliferation in vitro. RMS might be useful as an adjuvant tool in combination with stem cell therapy without adverse effects.

• 분석과학
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• 제31권1호
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• pp.7-13
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• 2018

이 연구에서는 구석기 고토양층 석영의 시간분해 광자극 냉광 신호를 측정한 후 측정된 냉광신호로부터 시료의 냉광수명을 산출하였다. 냉광수명의 방사선 조사 후 열전처리 의존성 및 조사선량 의존성, 광자극 펄스 의존성을 모두 고려한 결과, 고토양층 석영의 시간분해 광자극 냉광은 방사선량 100 Gy 조사 후 별도의 열전처리 없이 펄스 주기 $250{\mu}s$, 펄스 폭 $10{\mu}s$, 반복수 100,000번인 광 자극 펄스를 가하여 측정할 때 정확한 냉광수명이 산출됨을 알 수 있었다. 냉광수명의 측정온도 의존성으로부터 산출된 열소광 활성화 에너지 ${\Delta}E$, thermal assistance 활성화 에너지 $E_a$는 각각 $0.60{\pm}0.14eV$, $0.053{\pm}0.029eV$ 이었으며, 이 결과는 기존에 보고된 연구 성과와 매우 잘 일치하였다. 결론적으로 이 연구에서 산출된 고토양층 석영의 운동학 변수 값은 매우 신뢰할 수 있다고 판단된다.

• Journal of Acupuncture Research
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• 제18권4호
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• pp.116-124
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• 2001

Background and Objetive : The aim of this study was to investigate the effect of various electroacupuncture stimulation on NADPH-diaphorase in cerebral cortex, brain stem, cerebellum of spontaneously hypertensive rats. Materials and Methods : We evaluated the changes of NADPH-d-positive neurons using a histochemical method. The staining intensity of NADPH-d-positive neurons was assessed in a quantitative fashion using a microdensitometrical method based on optical density by means of an image analyzer. Results and Conculsion : The average optical density of NADPH-d-positive neurons of 100 Hz (bipolar square wave 0.2 ms duration and 100 Hz frequency) electroacupuncture treatment group significantly increased in most cortical areas comparison between the manual acupuncture and 2 Hz (bipolar square wave 0.2 ms duration and 2 Hz frequency) electroacupuncture groups. In the brain stem, the optical density of NADPH-d-positive neuron at only superficial gray layer of the superior colliculus area was same as cerebral cortex. We conclude that the morphological evidence for NADPH-d-positive neurons may be have regional change in cerebral cortex brain stem and cerebellum according to various electroacupuncture stimulations.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1991년도 춘계학술대회
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• pp.28-30
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• 1991

뇌의 시세포를 전기적으로 자극할때 작고 환한 섬광을 보게된다. 이 원리를 이용하여 전기 핀 배열판으로 축구 경기장의 전광판 같은 이미지를 만들어 맹인을 위한 기능적 시각, 걷기 혹은 글 읽기를 가능케 할 수 있다. 유타대학 생체공학과는 인공시각 연구에서 최고의 역사를 가지고 있다. 최근에 개발된 실리콘 전극 배열판은 가까운 장래의 인체 실험을 남겨놓고 있다.

• The Journal of Korean Physical Therapy
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• 제17권2호
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• pp.11-22
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• 2005

Purpose: The purpose of this study was to investigate the effects of aquatic health exercise program for the old. Methods: 20 healthy female with an average age of 71years($71{\pm}4.2$), were participated in this study. The aquatic health exercise program that include warm-up, stretching, strengthening exercise, WATSU and cool-down was performed in the pool two times a week for eight weeks(40min per one session). Body composition, strength of the both knee flexors and extensors, balance ability(sway area and path), whole body reaction time and flexibility(forward reaching test in long sitting) were measured before and after exercise. The data was analyzed with paired t-test to determine significant differences of all suggested factors between pre and post-exercise by make use of the SPSS(ver 10.0) package program. Results: The strength of the both knee flexors and extensors were increased significantly(right knee; p<0.01, left knee; p<0.05). Anteroposterior sway area (p<0.01), and sway path (p<0.001) of both leg were reduced significantly with eyes closed and opened. Whole body reaction time by optical stimulation was increased significantly (p<0.01) but whole body reaction time by auditory stimulation was no significant difference. The flexibility was increased significantly (p<0.01). Conclusion: Aquatic health exercise program can improve muscle strength, balance, whole body reaction, and flexibility.

• 대한의용생체공학회:의공학회지
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• 제26권3호
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• pp.145-150
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• 2005

A new gait detection system using both FSR (force sensing resistor) sensors and a gyrosensor was developed to detect various gait patterns. FSR sensors were put in self-designed shoe insoles and a gyrosensor was attached to the heel of a shoe. An algorithm was also developed to determine eight different gait transitions during four gait phases: heel-strike, foot-flat, heel-off and swing. The developed system was evaluated from nine heathy mans and twelve hemiplegic patients. Healthy volunteers were asked to walk in various gait patterns: level walking, fore-foot walking and stair walking. Only the level walking was performed in hemiplegic patients. The gait detection system was compared with a optical motion analysis system and the outputs of the FSR sensors. In healthy subjects, the developed system detected successfully more than $99\%$ for both level walking and fore-foot walking. For stair walking, the successful detection rate of the system was above$97\%$. In hemiplegic patients, the developed system detected approximately 98% of gait transitions. The developed gait phase detection system will be helpful not only to determine pathological gait phases but also to apply prosthetics, orthotics and functional electrical stimulation for patients with various gait disorders.

• 한방재활의학과학회지
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• 제20권1호
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• pp.27-36
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• 2010

목 적 : 본 연구는 전침 자극에 의한 척수내 N-methyl-D-aspartate(NMDA) 수용체의 NR1 및 NR2B subunit 인산화에 미치는 세포내 칼슘 저해제 bis-(2-aminophenoxy)-ethane-N,N,N',N'-tetraaceticacid(BAPTA)의 영향을 조사하였다. 방 법 : 인체의 족삼리(足三里)(ST36)와 삼음교(三陰交)(SP6)에 해당하는 혈자리에 2 Hz전침 자극을 1.0mA 세기로 30분 동안 자극하였다. 결 과 : 전침 무통각을 측정한 결과 높은 농도의 BAPTA 복강내주사 처리군에서 저하가 관찰되었다. 전침 처치 후 60분 후 분리한 $L_{4-5}$ 척수 분절에서 C-fos 발현 신경세포 수는 BAPTA 처리에 의해 감소하는데 특히 고유핵에서 저하가 현저하였다. 평균 integrated optical density로 비교한 NR1과 NR2B subunit에 대한 면역조직화학적 발현을 보면 전침 자극은 정상군에 비해 얕은 층판에서 증가하였다. NR1과 NR2B subunit의 인산화형에 대한 발현을 보면 NR1 인산화형은 척수 배각 전 부위에서, NR2B 인산화형은 얕은 층판에서 증가하였으며 BAPTA 처리에 의해 NR1 인산화형은 얕은 층판과 목 부위에서 NR2B 인산화형은 얕은 층판에서 현저한 감소를 보였다. western blot로 살펴본 BAPTA 처리에 의한 NR1 및 NR2B 인산화형 변화는 면역조직화학적 방법과 유사한 결과를 보여 주었다. 결 론 : 전침 무통각은 세포내 칼슘에 의한 척수 내 NMDA 수용체의 인산화가 중요한 요인으로 작용할 가능성이 있다.