The Journal of Korean Physical Therapy

The Korean Society of Physical Therapy (대한물리치료학회)
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Effect of Repetitive Magnetic Stimulation on Proliferation and Viability of Adipose Tissue-Derived Stromal Cells

반복자기자극이 지방유래 중간엽 줄기세포 증식과 활성에 미치는 영향

  • Kim, Su-Jeong (Institute of Medical Science, Yeungnam University) ;
  • Park, Hea-Woon (Department of Rehabilitation Medicine, School of Medicine, Catholic University of Deagu) ;
  • Cho, Yun-Woo (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University) ;
  • Lee, Joon-Ha (Department of Biochemistry and Molecular Biology, Yeungnam University) ;
  • Seo, Jeong-Min (Institute of Biomedical Engineering, Yeungnam University) ;
  • Shin, Hyoun-Jin (Institute of Biomedical Engineering, Yeungnam University) ;
  • Kang, Jae-Hoon (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University) ;
  • Ahn, Sang-Ho (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University)
  • 김수정 (영남대학교 의과학연구소) ;
  • 박해운 (대구가톨릭대학교 의과대학 재활의학교실) ;
  • 조윤우 (영남대학교 의과대학 재활의학교실) ;
  • 이준하 (영남대학교 생화학분자생물학교실) ;
  • 서정민 (영남대학교 의료공학연구소) ;
  • 신현진 (영남대학교 의료공학연구소) ;
  • 강재훈 (영남대학교 의과대학 재활의학교실) ;
  • 안상호 (영남대학교 의과대학 재활의학교실)
  • Received : 2009.08.10
  • Accepted : 2009.09.11
  • Published : 2009.09.25
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Abstract

Purpose: TThis study examined the effect of repetitive magnetic stimulation (RMS) on the viability and proliferative response of human adipose tissue-derived stromal cells (hATSCs) in vitro. Methods: The hATSCs were cultured primarily from human adipose tissue harvested by liposuction and incubated in a $37^{\circ}C$ plastic chamber. The cells were exposed to a repetitive magnetic field using a customized magnetic stimulator (Biocon-5000, Mcube Technology). The RMS parameters were set as follows: repetition rate=10Hz, 25Hz (stimulus intensity 100%= 0.1 Tesla, at 4cm from the coil), stimulated time= 1, 5, and 20 minutes. Twenty four hours after one application of RMS, the hATSCs were compared with the sham stimulation, which were kept under the same conditions without the application of RMS. The cells were observed by optical microscopy to determine the morphology and assessed by trypan blue staining for cell proliferation. The apoptosis and viability of the hATSCs were also analyzed by fluorescence-activated cell sorting (FACS) analysis of Annexin V and MTT assay. Results: After RMS, the morphology of the hATSCs was not changed and the apoptosis of hATSCs were not increased compared to the sham stimulation. The viability of the cells was similar to the cells given the sham stimulation. Interestingly, the level of hATSC proliferation was significantly higher in all RMS groups. Conclusion: The application of RMS may not cause a change in morphology and viability of hATSCs but can increase the level of cell proliferation in vitro. RMS might be useful as an adjuvant tool in combination with stem cell therapy without adverse effects.

Keywords

References

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