• 제목/요약/키워드: optical density

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활혈거어약(活血祛瘀藥)이 질내(膣內) 미생물(微生物)에 미치는 영향(影響) (Antibiotic Effects of blood-activating stasis-dispelling medicinals on Vaginal Microorganisms)

  • 조정훈;장준복;이경섭;류갑순
    • 대한한방부인과학회지
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    • 제19권4호
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    • pp.17-32
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    • 2006
  • Purpose : The aim of this study is to investigate the antibiotic effects of 14 herbs among blood-activating stasis-dispelling medicinals on vaginal microorganisms. Methods : Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Candida albicans, and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Lactobacillus gasseri, Streptococcus spp. and Escherichia coli HB101 were used for vaginal normal flora. The blood-activating stasis-dispelling medicinals, Mucunae Caulis, Salviae Radix, Persicae Semen, Myrrha, Zedoariae Rhizoma, Achuranthis Radix, Leonuri Herba, Melandrii Herba, Gleditsiae Spina, Lycopi Herba, Scirpi Rhizoma, Caesalpiniae Lignum, Corydlais Tuber and Polygoni Cuspidati Radix were used in this study. In vitro antibiotic activities were observed by optical density and colony test. Results : The optical density and colony test showed that Gleditsiae Spina, Scirpi Rhizoma, Corydlais Tuber, Polygoni Cuspidati Radix and Melandrii Herba of herbs among blood-activating stasis-dispelling medicinals had antimicrobial effect. Gleditsiae Spina had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis and MRSA. Scirpi Rhizoma had antimicrobial susceptibility and selective toxicity in Staphylococcus aureus and MRSA. Corydlais Tuber had antimicrobial susceptibility and selective toxicity in MRSA. Polygoni Cuspidati Radix had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis, Staphylococcus aureus and MRSA. Melandrii Herba had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis. Conclusion : According to the above results, we could suggest that Gleditsiae Spina, Scirpi Rhizoma, Corydlais Tuber, Polygoni Cuspidati Radix and Melandrii Herba of herbs among blood-activating stasis-dispelling medicinals be available to antimicrobial agent of vaginal pathogenic microbial species in vitro.

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평판형 고밀도 유도결합 건식 식각시 Optical Emission Spectroscopy를 이용한 $BCl_3$$BCl_3$/Ar 플라즈마의 분석 (Diagnosis of $BCl_3$ and $BCl_3$/Ar Plasmas with an Optical Emission Spectroscopy during High Density Planar Inductively Coupled Dry Etching)

  • Cho, Guan-Sik;Wantae Lim;Inkyoo Baek;Seungryul Yoo;Park, Hojin;Lee, Jewon;Kuksan Cho;S. J. Pearton
    • 한국재료학회:학술대회논문집
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    • 한국재료학회 2003년도 춘계학술발표강연 및 논문개요집
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    • pp.88-88
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    • 2003
  • Optical Emission Spectroscopy(OES) is a very important technology for real-time monitoring of plasma in a reactor during dry etching process. OES technology is non-invasive to the plasma process. It can be used to collect information on excitation and recombination between electrons and ions in the plasma. It also helps easily diagnose plasma intensity and monitor end-point during plasma etch processing. We studied high density planar inductively coupled BCl$_3$ and BCl$_3$/Ar plasma with an OES as a function of processing pressure, RIE chuck power, ICP source power and gas composition. The scan range of wavelength used was from 400 nm to 1000 nm. It was found that OES peak Intensity was a strong function of ICP source power and processing pressure, while it was almost independent on RIE chuck power in BCl$_3$-based planar ICP processes. It was also worthwhile to note that increase of processing pressure reduced negatively self-induced dc bias. The case was reverse for RIE chuck power. ICP power and gas composition hardly had influence on do bias. We will report OES results of high density planar inductively coupled BCl$_3$ and BCl$_3$/Ar Plasma in detail in this presentation.

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년근별 인삼추출물의 사포닌함량, 물리성 및 색도의 변화 (Variation of Effective Constituents Contents, Physical Properties and Color Intensities of Extracts from White Ginseng Roots of Different Cultivating Years.)

  • 김동철;장상문;최정
    • Applied Biological Chemistry
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    • 제38권1호
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    • pp.67-71
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    • 1995
  • 인삼추출물의 품질은 유효성분인 사포닌의 함량뿐만 아니라 추출물의 물리성 및 색도도 주요한 요인이다. 따라서 년근별 원료삼에서 추출한 추출물의 사포닌함량, 물리성 및 색도를 조사함으로써, 인삼추출물 제조시 우량원료삼을 선택을 위한 기초자료를 제시하고자 본 연구를 수행하였다. 원료삼의 년수가 증가할수록 추출물 중의 전당 및 sucrose의 함량과 추출물의 수율은 증가하였으나 조saponin함량은 년근 간에 유의성이 없었다. 조회분함량은 4년근의 추출물에서 가장 많았으며, 5년근의 추출물에서 가장 낮았다. 또한 인삼추출물의 탁도는 인삼근의 년수가 증가할수록 감소되었으나, 점도는 년근별 유의차가 인정되지 않았다. 갈변반응의 중간생성물의 흡광도는 인삼근의 년수가 증가할수록 감소되었으며, 5년근 추출물의 명도 및 황색도가 가장 높았다.

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청열약(淸熱藥)의 질내(膣內) 미생물(微生物)에 대(對) 항균효과(抗菌效果) (Antibiotic Effect of Heat-Clearing Medicinals on Vaginal Microorganisms)

  • 조정훈;장준복;이경섭;김경직
    • 대한한방부인과학회지
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    • 제19권3호
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    • pp.175-190
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    • 2006
  • Purpose : This study was conducted to investigate the in vitro inhibitory effects of heat-clearing medicinal on common bacterias in gynecology. Methods : The heat-clearing medicinals ( Trichosanthis Radix, Sophorae Fructus, Phragmitis Rhizoma, Buddleiae Flos, Bambusae Folium, Anemarrhenae Rhizoma, Celosiae Semen, Gardeniae Fructus, Prunellae Spica, Sophorae Radix, Dictamni Radicis Cortex, Coptidis Rhizoma, Gentianae Scabrae Radix, Scutellariae Radix, Phellodendri Cortex) were used in this study. Staphylococcus aureus, MRSA, Candida albicans and Gardnerella vaginalis were used for vaginal pathogenic microorganisms. Streptococcus spp., Escherichia coli HB101, Lactobacillus gasseri were used for normal vaginal florae. We evaluated antibiotic effect by the optical density and the colony test. Results : The optical density and colony test showed that Celosiae Semen, Prunellae Spica, Scutellariae Radix and Phellodendri Cortex of herbs among heat-clearing medicinal had antimircobial effect. Celosiae Semen and Prunellae Spica had antimicrobial susceptibility and selective toxicity in MRSA. Scutellariae Radix and Phellodendri Cortex had antimicrobial susceptibility and selective toxicity in Gardnerella vaginalis. Conclusion : According to the above results, we could suggest that Celosiae Semen, Prunellae Spica, Scutellariae Radix and Phellodendri Cortex among heat-clearing medicinal be available to antimicrobial agent of vaginal pathogenic microbial species in vitro.

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효소면역법에 의한 닭 전염성 후두기관염 바이러스 항체 측정에 관한 연구 (Detection of Antibody to Infectious Laryngotracheitis Virus by Enzyme Linked Immunosorbent Assay)

  • 임숙경;위성하;최정옥;고홍남
    • 한국동물위생학회지
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    • 제15권1호
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    • pp.32-45
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    • 1992
  • In order to establish and enzyme-linked immunosorbent assay to ILTV, field virus strain of ILTV was propagated in chorioallantoic membrane of the embryonated eggs. purified and used as antigen. The antisera selected from the field samples and immunized chickens based on serum neutralization test were used as the standard positive and negative sera in all tests. It was found that optimal antigen concentration was $2{\mu}g$ of protein per well and a 1 : 100 dilution of standard serum showed low background optical density with negative serum and high P/N values of positive sera. A 1 : 500 dilution of the rabbit anti-chicken IgG peroxidase conjugate produced a high P/N values and thirty minutes was chosen as suitable time to read the optical density of the enzyme substrate reaction and optical density was consistent during the 16 hours after stopper was treated. When coated antigen was kept on microplate for varying time up to 16 hours at $4^{\circ}C$ or $37^{\circ}C,$ no significant difference was observed between the treatment. The coated antigen could be kept without change of antigenicity for at least one month at $-70^{\circ}C,\; -20^{\circ}C,\; 4^{\circ}C$ and room temperature. When blocking buffer contanining bovine serum albumin was mixed directly with conjugate and serum at 10% level induced higher P/N values compared to blocking antigen coated microplate with the same blocking buffer. The coefficience of correlation between ELISA and SN test was 0.577. When antibody response of chickens, vaccinated with ILTV, was examined by ELISA and SN test, antibody rising and decay pattern between the two test was similar until 11 weeks of age. However 12 weeks onward antibody titer checked on by SN test was slightly lower than that tested by ELISA.

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Differential changes of nicotinamide adenine dinucleotide phosphate-diaphorase, neuropeptide Y and vasoactive intestinal peptide in the cerebral cortex of the rat after repeated electroacupuncture

  • Kim, Yong-Suk;Kim, Jong-In;Kim, Chang-Hwan;Yoo, Jin-Hwa;Huh, Young-Buhm
    • Journal of Acupuncture Research
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    • 제22권2호
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    • pp.13-18
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    • 2005
  • This study was undertaken to investigate the effects of electroacupuncture(EA) on Choksamni(ST36), a well-known acupuncture site, on nicotinamide adenine dinucleotide phosphate-diaphorase(NADPH-d), neuropeptide Y(NPY) and vasoactive intestinal peptide(VIP) in the cerebral cortex of spontaneously hypertensive rats(SHR). EA on Choksamni was applied using 2Hz electrical biphasic pulses of 10min, 3 times a week for a total of 10 sessions. Thereafter we evaluated changes in NADPH-d-positive neurons histochemically and changes in NPY and VIP-positive neurons immunohistochemically. The optical density of NADPH-d-positive neurons in the Choksamni group was significantly lower in all areas of the cerebral cortex than in the control group. However, the optical density of NPY-positive neurons in the Choksamni group was similar to that of the controls in most areas of the cerebral cortex, with the exception of the primary motor and visual cortices. The optical density of VIP-positive neurons in the Choksamni group was significantly decreased as compared to the control group in most areas of the cerebral cortex, with the exception of the cingulate cortex. The present results demonstrated that EA on Choksamni changes the activity of the NO system, and that stimulation at the same level, causes selective changes within the peptidergic system in the cerebral cortex of SHR.

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Statistical Characteristics of Polarization - Sensitive Optical Coherence Tomography for Tissue Imaging

  • Oh, Jung-Taek;Kim, Beop-Min;Kim, Seung-Woo
    • Journal of the Optical Society of Korea
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    • 제7권4호
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    • pp.211-215
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    • 2003
  • Statistical characteristics of the backscattered light from turbid tissues obtained by polarizationsensitive optical coherence tomography are investigated. The amplitude of the backscattered light is found to faithfully follow the Rayleigh distribution predicted by the scattering theory of electromagnetic waves in random media. The probability density function of the phase difference between the two orthogonal polarization components of the backscattered light is explicitly derived and then verified in comparison with the experimental data measured from in-vitro tissues of porcine ligament.

광 디스크 드라이브의 강인한 데이터-클럭 등화기 필터계수 선정 (A Method of Selecting Filter Coefficient for Robust Data to Clock Equalizer in Optical Disc Drive)

  • 염동회;김진규;주영훈
    • 전기학회논문지
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    • 제59권4호
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    • pp.793-796
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    • 2010
  • The equalizer compensates a signal distorted by transmission lines and amplifying stages, so the signal can have uniform characteristics over all frequency range. The equalizer in ODD(Optical Disc Drive) improves the stability of the extracted clock from a received signal and the readability of an inserted disc by suppressing noise and ISI(Inter-Symbol Inference). The length of marks-spaces and track pitch on discs becomes shorter as the recording density of an optical media is higher, which causes noise and ISI. And, the sensitivity about the fluctuation of physical systems is higher as the optical devices become more complicate. This paper proposes a method to select the coefficient of built-in equalizer of ODD in order to maintain the quality of signals against noise and ISI caused by system fluctuation.

단열층이 DVD 기판의 복굴절 및 전사성에 미치는 영향 (Effect of Insulation Layer on Birefringence and Land-groove Pattern in DVD-RAM Substrate)

  • 김영민;성기병;강신일
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2001년도 춘계학술대회논문집C
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    • pp.432-437
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    • 2001
  • It is necessary to improve mechanical and optical porperties in the optical disk substrates as the information storage devices with high storage density using blue laser are being developed. Injection compression molding is regarded as the most suitable process to manufacture optical disk substrates for quality recording and read-out. In the present research, the effects of processing conditions and the insulation layer thickness on gapwise birefringence and the land-groove pattern were investigated. It was found that the values of the birefringence distribution were very sensitive to mold temperature history, and the level of birefringence reduced and, furthermore, the quality of replication was improved due to the insulation layer.

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치수공차가 고려된 방진마운트의 동특성 예측 (Prediction of Dynamic Characteristics of Rubber Mount far Anti-Vibration Considering the Dimensional Tolerance)

  • 김국원;김남웅
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 2002년도 춘계학술대회 논문집
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    • pp.829-832
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    • 2002
  • With the increase of storage density, high rotational speed and high access technologies in optical disk drive, mechanical issues, mainly noise and vibration, become critical. Up to now the researches of rubber mount for anti-vibration focused on how to calculate the static and the dynamic stiffness of rubber mount and leaved out of consideration of the dimensional tolerance of rubber mount for anti-vibration. This paper presents the effects of dimensional tolerance of rubber mount for anti-vibration on the dynamic characteristics of optical disk drive by finite element analysis and dynamic test. The relation between dimensional tolerance and dynamic characteristics of optical disk drive is observed and discussed.

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