• Journal of Microbiology and Biotechnology
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• v.23 no.11
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• pp.1500-1508
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• 2013

Ebosin, a novel exopolysaccharide produced by Streptomyces sp. 139, has obvious antirheumatic arthritis activity in vivo, and its biosynthesis gene cluster (ste), consisting of 27 open reading frames, has been identified. This paper reports our study of the gene functionality of ste8, the predicted protein product of which is homologous to some bacterial chain length determinant Wzz proteins. For characterization of Ste8, ste8 was cloned and expressed in the mutant strain E. coli 086:H2 (${\Delta}wzz$). The functional complementation of wzz by ste8 was demonstrated by the restoration of wild-type lipopolysaccharide biosynthesis and increased levels of serum resistance of E. coli 086:H2 (${\Delta}wzz$) (pET30a-ste8). To examine the function of ste8 in ebosin biosynthesis, the gene was knocked out with a double crossover via homologous recombination. The molecular weight of the ebosin derivative EPS-8m produced by the mutant Streptomyces sp. 139 ($ste8^-$) was much lower than that of ebosin, and the binding activity of EPS-8m for IL-1R decreased significantly compared with ebosin. These results demonstrate that ste8 encodes a chain length determinant (Wzz) that functions in ebosin biosynthesis.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.17 no.4
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• pp.86-98
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• 2018

Since 2015, the Korea Expressway Corporation has provided predicted travel time information, which is reproduced from DSRC systems over the extended expressway network in Korea. When it is open for public information, it helps travelers decide optimal routes while minimizing traffic congestions and travel cost. Although, sutiable evaluations to investigate the reliability of travel time forecast information have not been conducted so far. First of all, this study seeks to find out a measure of effectiveness to evaluate the reliability of travel time forecast via various literatures. Secondly, using the performance measurement, this study evaluates concurrent travel time forecast information in highway quantitatively and examines the forecast error by exploratory data analysis. It appears that most of highway lines provided reliable forecast information. However, we found significant over/under-forecast on a few links within several long lines and it turns out that such minor errors reduce overall reliability in travel time forecast of the corresponding highway lines. This study would help to build a priority for quality control of the travel time forecast information system, and highlight the importance of performing periodic and sustainable management for travel time forecast information.

• Journal of the Korean Geographical Society
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• v.51 no.1
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• pp.143-166
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• 2016

This study aims to consider spiritual therapeutic landscapes thought the case of catholic sacred places in Korea. The catholic sacred places, which have been a series of persecution, are distributed in 300 all over the country and can be divided into a formational type, a selective type, and a participatory type. In the formational type, the catholic sculptures are scattered here and there; but it seems that there are limits somewhat to feel the emotional stability and the experience of caring by God. Because the formational type is deserted or is built up in unnoticeable urban areas. The religious factors, for example, museums, special pray, Station of the Cross, Rosary way, statues of the Virgin Mary, catholic sculptures, etc. in the selective type are placed in the corners of the catholic sacred places to provide the opportunity of holy experience to pilgrims. But the level and the intensity of healing depends on the matter as to how much pilgrims experience religious factors voluntarily. Lastly, the participatory type means the experience of various religious factors following scheduled programs. The experience is for the group of pilgrims, not a person; and the human relationship via the religious experience spiritualizes the network of interest towards other participants. Furthermore, the spiritualized open mind towards other people becomes the power that can arrive at the emotional stability, the relief of stress, and the in-depth and authentic healing. Ultimately, the spiritual healing through religious factors is to realize human finitude and dedicate one's own self to God; in addition, the spiritual therapeutic landscapes are the place that can carry out such a spiritual ritual without any restraint.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.36 no.9
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• pp.931-935
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• 2012

In this study, we fabricate and investigate low-temperature solid oxide fuel cells with a ceramic substrate/porous metal/ceramic/porous metal structure. To realize low-temperature operation in solid oxide fuel cells, the membrane should be fabricated to have a thickness of the order of a few hundreds nanometers to minimize IR loss. Yttrium-doped barium zirconate (BYZ), a proton conductor, was used as the electrolyte. We deposited a 350-nm-thick Pt (anode) layer on a porous substrate by sputter deposition. We also deposited a 1-${\mu}m$-thick BYZ layer on the Pt anode using pulsed laser deposition (PLD). Finally, we deposited a 200-nm-thick Pt (cathode) layer on the BYZ electrolyte by sputter deposition. The open circuit voltage (OCV) is 0.806 V, and the maximum power density is 11.9 mW/$cm^2$ at $350^{\circ}C$. Even though a fully dense electrolyte is deposited via PLD, a cross-sectional transmission electron microscopy (TEM) image reveals many voids and defects.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.7
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• pp.829-835
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• 2010

This paper presents a disposable power generator for microfluidic devices; the power generator has a tubular PEMFC and a $H_2$ generator. The tubular PEMFC has a tubular MEA (diameter: 1.52 mm) that is supported by a spiral wire electrode. The $H_2$ generator supplied $H_2$ to the tubular PEMFC; $H_2$ was generated via the reaction of Al foil (27 mg) and 5 M NaOH (0.12 ml). The open circuit voltage and power density of a unit cell of the tubular PEMFC were 0.81 V and $16.4\;mW/cm^2$ (0.35 V), respectively. The $H_2$ generator generated 11.6 ml $H_2$ for 15min. The power generator was continuously operated for 15 min at 0.64 mW (0.71 V) and for 10 min at 1.06 mW (0.46 V). We experimentally verified that it is feasible to use the proposed power generator as a power source for microfluidic devices; in the experiment, an LED (2.5 mW; 1.8 V) was lit for 10 min by using three serially connected TPEMFCs and one $H_2$ generator.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.11 no.6
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• pp.235-245
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• 2011

EBooks refer to electronic versions of books which are accessible via internet with forms of digital texts. In recent years, Amazon's Kindle digital eBooks has revealed the possibilities of success of the e-book market, which leads other companies to launch eBook service such as Google's eBook stores and Apple's iPad and eBook service. These reveal that the eBook market is finally showing a substantial amount of growth. Although the issue of technical support of eBook copyright protection emerges from the fast growing eBook marketplace, current technic of commercial DRM for protecting eBook copyright protection still has problems of non-comparability. Therefore, with the current technical status, DRM comparability problems, which have already occurred in music DRM environment, would also happen in eBook environment. This study suggests a standard framework to support eBook DRM comparability. When development of the standard reference software for eBook DRM comparability is completed, the sources will be registered as shareware to be open to public.

• Bulletin of the Korean Chemical Society
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• v.32 no.2
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• pp.417-423
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• 2011

A series of new quinoxaline-based thiophene copolymers (PQx2T, PQx4T, and PQx6T) was synthesized via Yamamoto and Stille coupling reactions. The $M_ws$ of PQx2T, PQx4T, and PQx6T were found to be 20,000, 12,000, and 29,000, with polydispersity indices of 2.0, 1.2, and 1.1, respectively. The UV-visible absorption spectra of the polymers showed two distinct absorption peaks in the ranges 350 - 460 nm and 560 - 600 nm, which arose from the ${\pi}-{\pi}^*$ transition of oligothiophene units and intramolecular charge transfer (ICT) between a quinoxaline acceptor and thiophene donor. The HOMO levels of the polymer ranged from -5.37 to -5.17 eV and the LUMO levels ranged from -3.67 to -3.45 eV. The electrochemical bandgaps of PQx2T, PQx4T, and PQx6T were 1.70, 1.71, and 1.72 eV, respectively, thus yielding low bandgap behavior. PQx2T, PQx4T, and PQx6T had open circuit voltages of 0.58, 0.42, and 0.47 V, and short circuit current densities of 2.9, 5.29 and 9.05 mA/$cm^2$, respectively, when $PC_{71}BM$ was used as an acceptor. For the solar cells with PQx2T-PQx6T:$PC_{71}BM$ (1:3) blends, an increase in performance was observed in going from PQx2T to PQx6T. The power conversion efficiencies of PQx2T, PQx4T, and PQx6T devices were found to be 0.69%, 0.73%, and 1.80% under AM 1.5 G (100 mW/$cm^2$) illumination.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.20 no.2
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• pp.113-125
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• 2007

Improved numerical method to obtain the exact stiffness matrices is newly proposed to perform the spatially coupled elastic and stability analyses of non-symmetric and open/closed thin-walled beam on elastic foundation. This method overcomes drawbacks of the previous method to evaluate the exact stiffness matrix for the spatially coupled stability analysis of thin-walled beam-column This numerical technique is accomplished via a generalized eigenproblem associated with 14 displacement parameters by transforming equilibrium equations to a set of first order simultaneous ordinary differential equations. Next polynomial expressions as trial solutions are assumed for displacement parameters corresponding to zero eigenvalues and the eigenmodes containing undetermined parameters equal to the number of zero eigenvalues are determined by invoking the identity condition. And then the exact displacement functions are constructed by combining eigensolutions and polynomial solutions corresponding to non-zero and zero eigenvalues, respectively. Consequently an exact stiffness matrix is evaluated by applying the member force-deformation relationships to these displacement functions. In order to illustrate the accuracy and the practical usefulness of this study, the numerical solutions are compared with results obtained from the thin-walled beam and shell elements.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.575-584
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• 2015

Drought stress is a crucial environmental factor determining crop survival and productivity. The goal of this study was to clearly identify a new drought stress-tolerance gene in Brassica rapa. From KBGP-24K microarray data with the B. rapa ssp. pekinensis inbred line 'Chiifu' under drought stress treatment, a gene which was named BrDSR (B. rapa Drought Stress Resistance) was chosen among 738 drought-responsive unigenes. BrDSR function has yet to be determined, but its expression was induced over 6-fold by drought. To characterize BrDSR, the gene was isolated from B. rapa inbred line 'CT001' and found to contain a 438-bp open reading frame encoding a 145 amino acid protein. The full-length cDNA of BrDSR was used to construct an over-expression vector, 'pSL100'. Tobacco transformation was then conducted to analyze whether the BrDSR gene can increase drought tolerance in plants. The BrDSR expression level in T1 transgenic tobacco plants selected via PCR and DNA blot analyses was up to 2.6-fold higher than non-transgenic tobacco. Analysis of phenotype clearly showed that BrDSR-expressing tobacco plants exhibited more tolerance than wild type under 10 d drought stress. Taking all of these findings together, we expect that BrDSR functions effectively in plant growth and survival of drought stress conditions.

• Korean Security Journal
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• no.31
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• pp.125-155
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• 2012

Terrorist organization has shown the trend of secret organization and it is harder to cope with terrorism because of uncertainty of terrorism. Homegrown terrorism is the one, whose preparation, execution and effect are restricted to domestic area. By the way, in the worldwide economic depression, violence and radical demonstration have shown the expansion trends as in Middle East, political revolution of Africa, anti-social resistance of Europe and Wall Street Occupation of USA. Homegrown terrorism is occurring in various countries such as UK and Spain as well as USA. Specialists warn homegrown terrorism in Korea. The purpose of this study was to prospect the possibility of homegrown terrorism that can be generated in the transfer to multi-culture society as various foreigners come to Korea rapidly and suggest the method to cope with the trend. The study analyzed environment and analysis of homegrown terrorism that Korea faces now. The methods to cope with homegrown terrorism are as follows. First, distribution of radical homegrown terrorism via internet should be prevented. Second, the connection between terrorist organization and homegrown terrorist should be prevented. Third, there should be a cooperation among government, residents and religious group. Fourth, there should be an open approach against multi-culture society. Fifth, there should be a systematic control for cause of new conflict. Finally, there should be a long-term approach to cause of new conflict. If we do not make an effort to prevent homegrown terrorism, terrorism environment may face new aspect and national and social cost for it will increase.