• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1361-1365
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• 2015

Background: We aimed to discover potential gene biomarkers for gastric cancer (GC) diagnosis. Materials and Methods: Genechips of 10 GC tissues and 10 gastric mucosa (GM, para-carcinoma tissue, normal control) tissues were generated using an exon array of Affymetrix containing 30,000 genes. The differentially expressed genes (DEGs) between GC tissues and normal control were identified by the Limma package and analyzed by hierarchical clustering analysis. Gene ontology (GO) and pathway enrichment analyses were performed for investigating the functions of DEGs. Receiver operating characteristics (ROC) analysis was performed to measure the effects of biomarker candidates for diagnosis of GC. Results: Totals of 896 up-regulated and 60 down-regulated DEGs were identified to be differentially expressed between GC samples and normal control. Hierarchical clustering analysis showed that DEGs were highly differentially expressed and most DEGs were up-regulated. The most significantly enriched GO-BP term was revealed to be mitotic cell cycle and the most significantly enriched pathway was cell cycle. The intersection analysis showed that most significant DEGs were cyclin B1 (CCNB1) and cyclin B2 (CCNB2). The sensitivities and specificities of CCNB1 and CCNB2 were both high (p<0.0001). Areas under the ROC curve for CCNB1 and CCNB2 were both greater than 0.9 (p<0.0001). Conclusions: CCNB1 and CCNB2, which were involved in cell cycle, played significant roles in the progression and development of GC and these genes may be potential biomarkers for diagnosis and prognosis of GC.

• The Journal of Society for e-Business Studies
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• v.12 no.3
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• pp.1-17
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• 2007

A virtual enterprise (VE) can be mapped into a multi-agent system (MAS) that consists of various agents with specific role(s), communicating with each other to accomplish common goal(s). However, a MAS for enacting VE requires more advanced mechanism such as context that can guarantee autonomy and dynamism of VE members considering heterogeneity and complex structure of them. This paper is to suggest a context-based MAS as a platform for constructing and managing virtual enterprises. In the Context-based MAS a VE is a collection of Actor, Interaction (among Actors), Actor Context, and Interaction Context. It can raise the speed and correctness of decision-making and operation of VE enactment using context, i.e., information about the situation (e.g., goal, role, task, time, location, media) of Actors and Interactions, as well as simple data of their properties. The Context-based MAS for VE we proposed('VECoM') may consists of Context Ontology, Context Model, Context Analyzer, and Context Reasoner. The suggested approach and system is validated through an example where a VE tries to find a partner that could join co-development of new technology.

• Molecules and Cells
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• v.39 no.10
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• pp.728-733
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• 2016

Mesenchymal stem cells (MSCs) effectively reduce airway inflammation and regenerate the alveolus in cigarette- and elastase-induced chronic obstructive pulmonary disease (COPD) animal models. The effects of stem cells are thought to be paracrine and immune-modulatory because very few stem cells remain in the lung one day after their systemic injection, which has been demonstrated previously. In this report, we analyzed the gene expression profiles to compare mouse lungs with chronic exposure to cigarette smoke with non-exposed lungs. Gene expression profiling was also conducted in a mouse lung tissue with chronic exposure to cigarette smoke following the systemic injection of human cord blood-derived mesenchymal stem cells (hCB-MSCs). Globally, 834 genes were differentially expressed after systemic injection of hCB-MSCs. Seven and 21 genes, respectively, were up-and downregulated on days 1, 4, and 14 after HCB-MSC injection. The Hbb and Hba, genes with oxygen transport and antioxidant functions, were increased on days 1 and 14. A serine protease inhibitor was also increased at a similar time point after injection of hCB-MSCs. Gene Ontology analysis indicated that the levels of genes related to immune responses, metabolic processes, and blood vessel development were altered, indicating host responses after hCB-MSC injection. These gene expression changes suggest that MSCs induce a regeneration mechanism against COPD induced by cigarette smoke. These analyses provide basic data for understanding the regeneration mechanisms promoted by hCB-MSCs in cigarette smoke-induced COPD.

• Journal of Korea Spatial Information System Society
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• v.12 no.1
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• pp.100-104
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• 2010

Recently with advent of Web 2.0, RESTful Web Services are becoming increasing trend to emphasize Web as platform. There are already many services and the number of service increases in very fast pace. So it is difficult to find the service what we want by keyword based search. To solve this problem, we developed the search method using sem antic technologies about RESTful Web Services. For that, first we define the system structure and model the description format based on the integrated search system for OpenAPIs, and then we add Semantic Markup (tagging, semantic annotation) on the HTML description pages. Next we extract RDF document from them and store it in service repository. Based on the keywords that are extended by means of ontology, the developed system provides more purified and extended results than similarity-based keyword searching system.

• The Plant Pathology Journal
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• v.30 no.3
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• pp.236-244
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• 2014

The plant pathogen Fusarium graminearum causes Fusarium head blight in cereal crops and produces mycotoxins that are harmful to animals and humans. For the initiation and spread of disease, asexual and sexual reproduction is required. Therefore, studies on fungal reproduction contribute to the development of new methods to control and maintain the fungal population. Screening a previously generated transcription factor mutant collection, we identified one putative $C_2H_2$ zincfinger transcription factor, pcs1, which is required for both sexual and asexual reproduction. Deleting pcs1 in F. graminearum resulted in a dramatic reduction in conidial production and a complete loss of sexual reproduction. The pathways and gene ontology of pcs1-dependent genes from microarray experiments showed that several G-protein related pathways, oxidase activity, ribosome biogenesis, and RNA binding and processing were highly enriched, suggesting that pcs1 is involved in several different biological processes. Further, overexpression of pcs1 increased conidial production and resulted in earlier maturation of ascospores compared to the wild-type strain. Additionally, the vegetative growth of the overexpression mutants was decreased in nutrient-rich conditions but was not different from the wild-type strain in nutrient-poor conditions. Overall, we discovered that the pcs1 transcription factor positively regulates both conidiation and sexual reproduction and confers nutrient condition-dependent vegetative growth.

• Journal of Periodontal and Implant Science
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• v.41 no.4
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• pp.192-200
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• 2011

Purpose: The aim of this study is to compare the gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow for characterization of dental stem cells. Methods: We employed GeneChip analysis to the expression levels of approximately 32,321 kinds of transcripts in 5 samples of bone-marrow-derived mesenchymal stem cells (BMSCs) (n=1), periodontal ligament stem cells (PDLSCs) (n=2), and dental pulp stem cells (DPSCs) (n=2). Each cell was sorted by a FACS Vantage Sorter using immunocytochemical staining of the early mesenchymal stem cell surface marker STRO-1 before the microarray analysis. Results: We identified 379 up-regulated and 133 down-regulated transcripts in BMSCs, 68 up-regulated and 64 down-regulated transcripts in PDLSCs, and 218 up-regulated and 231 down-regulated transcripts in DPSCs. In addition, anatomical structure development and anatomical structure morphogenesis gene ontology (GO) terms were over-represented in all three different mesenchymal stem cells and GO terms related to blood vessels, and neurons were over-represented only in DPSCs. Conclusions: This study demonstrated the genome-wide gene expression patterns of STRO-$1^+$ mesenchymal stem cells derived from dental tissues and bone marrow. The differences among the expression profiles of BMSCs, PDLSCs, and DPSCs were shown, and 999 candidate genes were found to be definitely up- or down-regulated. In addition, GOstat analyses of regulated gene products provided over-represented GO classes. These data provide a first step for discovering molecules key to the characteristics of dental stem cells.

• Journal of Digital Contents Society
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• v.10 no.1
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• pp.1-10
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• 2009

One important issue to generalize the ubiquitous paradigm is the development of user-centralized and intelligent ubiquitous computing systems. Sharing knowledge and correct communication between users and devices are needed to be aware of continuous changed context information and infer services for which users are suited. The goal of this paper is to describe and manage effectively the meaning of services or data which each device offers for interaction between users and devices based on semantic relationships and reasoning. In this paper, we represent semantic data using OWL and design a ubiquitous based intelligent system. We propose some index structures and strategies to process queries classified by each subsystem and adopt labeling schemes to identify classes and resources in the semantic data. We can find devices which satisfies various user's requests exactly and quickly using the proposed strategies.

• Korean Journal of Computational Design and Engineering
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• v.19 no.4
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• pp.390-401
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• 2014

ISO 15926 is an international standard for integration of lifecycle data for process plants including oil and gas facilities. From the viewpoint of information modeling, ISO 15926 Parts 2 provides the general data model that is designed to be used in conjunction with reference data. Reference data are standard instances that represent classes, objects, properties, and templates common to a number of users, process plants, or both. ISO 15926 Parts 4 and 7 provide the initial set of classes, objects, properties and the initial set of templates, respectively. User-defined reference data specific to companies or organizations are defined by inheriting from the initial reference data and the initial set of templates. In order to support the extension of reference data and templates, an editor that provides creation, deletion and modification functions of user-defined reference data is needed. In this study, an editor for reference data based on ISO 15926 was developed. Sample reference data were encoded in OWL (web ontology language) according to the specification of ISO 15926 Part 8. iRINGTools and dot15926Editor were benchmarked for the design of GUI (graphical user interface). Reference data search, creation, modification, and deletion functions were implemented with XML (extensible markup language) DOM (document object model), and SPARQL (SPARQL protocol and RDF query language).

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.23 no.2
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• pp.5-25
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• 2012

The purpose of this study is to develop linked data of authority data in national archives of Korea as a cornerstone for linked data cloud of Korea. The study analyzed data structure of authority data as well as a retrieval system. It finally developed linked data based on RDF/OWL, Dublin Core, and SKOS. The study also employed TopBraid ComposerTM as a tool for ontology construction. The visualization of the tool provides users with flexible search and browsing between data as well as access of detail authority data. It complements the search of a current system in terms of flexible linking between records and authority data. The study also suggests future work to publish linked data of archival data set itself and make rich relationships among data in museums, libraries, and other archives.

• Journal of Digital Contents Society
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• v.6 no.4
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• pp.201-208
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• 2005

In high-technology digital age that change everyday fast, Web became most familiar and essential tool to public. Since creation of web, web began from the first step web that offer simple information and contents. and now, it have developed to step offer dynamic contents that various multimedia components are combined. In future, it will develop to the next generation, intelligence style Semantic Web that Meta Communication of meaning-base is possible. In this paper, we examine about concept and characteristic of Semantic web that is a point technology in next generation. Also, we studied about example and practical use in personalized contents side that is based on Semantic Meta-Communication system. Semantic web is value industry field that has infinite possibility and has necessary of a lot of studies and development yet. Therefore in digital age, we expect this paper will help for space construction of more mature communication.