• 정보관리학회지
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• 제28권2호
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• pp.11-36
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• 2011

1990년대부터 계량정보학은 정보학자들 사이에서 주목을 받는 분야로 발전해오면서 현재 계량서지학, 사이언토메트릭스 등 모든 계량학을 포괄하는 개념으로 인식되고 있다. 계량정보학의 역동적인 발전을 조명하기 위하여 이 연구에서는 계량정보학 연구출판물을 기반으로 하여 이 분야의 지적구조를 분석하고자 하였다. 적용된 기법은 통계적 기법과 프로파일링 기법이다. 데이터 수집을 위해서는 SCI 데이터베이스를 이용하였으며 2001년부터 2011년까지의 Web of Knoewledge 데이터베이스에서 다음과 같은 5개의 질의를 가지고 데이터를 수집하였다: informetrics', bibliometrics', scientometrics', webometrics', citation analysis.' 프로파일링 기법으로 주요 주제, 저자, 저널별로 지적구조도를 제시하였으며 계량정보학 연구를 수행한 주요 국가, 기관, 저자도 분석되었는데 미국과 영국이 이 분야 연구를 주도하고 있으며 M. Thelwall이 10년동안 생산성이 가장 높은 저자인 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.5-14
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• 2008

The deep subseafloor rock in oil reservoirs represents a unique environment in which a high oil contamination and a very low biomass can be observed. Sampling this environment has been a challenge owing to the techniques used for drilling and coring. In this study, the facilities developed by the Brazilian oil company PETROBRAS for accessing deep subsurface oil reservoirs were used to obtain rock samples at 2,822-2,828 m below the ocean floor surface from a virgin field located in the Atlantic Ocean, Rio de Janeiro. To address the bacterial diversity of these rock samples, PCR amplicons were obtained using the DNA from four core sections and universal primers for 16S rRNA and for APS reductase (aps) genes. Clone libraries were generated from these PCR fragments and 87 clones were sequenced. The phylogenetic analyses of the 16S rDNA clone libraries showed a wide distribution of types in the domain bacteria in the four core samples, and the majority of the clones were identified as belonging to Betaproteobacteria. The sulfate-reducing bacteria community could only be amplified by PCR in one sample, and all clones were identified as belonging to Gammaproteobacteria. For the first time, the bacterial community was assessed in such a deep subsurface environment.

• 대한지역사회영양학회지
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• 제20권4호
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• pp.281-290
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• 2015

Objectives: Obesity is known to influence physical and mental health as well as the general quality of life. The aim of this study was to evaluate the effect of obesity related quality of life on selecting a goal for weight management in overweight and obese female patients. Methods: A total of 140 overweight or obese (Body mass index $23kg/m^2$) female outpatients aged ${\geq}20$ and ${\leq}60$ years from one clinic participated in this study. Patients' desired weight (goal weight, ideal weight, satisfactory weight, acceptable weight and disappointed weight) and obesity related quality of life measures were evaluated. Univariate and multivariate analysis were performed to evaluate the effect of obesity related quality of life on goal weight reduction (%) and goal body mass index (BMI). Results: Mean BMI of overweight group, mild obesity group and severe obesity group were $62.0{\pm}4.8kg$, $68.5{\pm}5.5kg$ and $83.5{\pm}9.6kg$, respectively. Mean weight loss expectations of the three groups were $16.4{\pm}4.7%$, $19.5{\pm}5.3%$ and $30.2{\pm}6.8%$, respectively and goal weight was significantly different among the three groups. Severe obesity group had a lower total quality of life score including physical, work-related, daily living domains than overweight or mild obesity groups. In univariate and multivariate regression analysis, psychosocial domain of quality of life had an effect on goal weight reduction (%) and goal BMI. Conclusions: The results of this study demonstrated that the obese patients with poorer quality of life and psychosocial health tended to choose higher goal weight reduction and lower goal BMI.

• 한국축산식품학회지
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• 제30권3호
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• pp.385-391
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• 2010

This study was conducted to compare proteins expressed in M. longissimus from Hanwoo and Holstein steers immediately after slaughter. Two-dimensional electrophoresis (2DE)/LC-MS/MS analysis revealed that the total number of detectable protein spots from longissimus muscle tissues was slightly higher in Hanwoo ($575{\pm}65$) than Holstein ($534{\pm}13$) steers, but that these numbers were not statistically significant due to large variation between replicates. A total of twelve protein spots did not match between sample groups, eight of which were expressed in the Hanwoo sample and four that were expressed in the Holstein sample. The protein spots detected in the Hanwoo sample included smooth muscle and non-muscle myosin alkali light chain 6B isomers, ${\alpha}B$ crystallin isomers, hemoglobin ${\beta}$-A chains, slow myosin heavy chains, and slow skeletal muscle troponin T chains. Collectively, these proteins are a class of slow-twitch muscle fiber and mirror that Hanwoo muscle tissue sampled for the current study contained more slow-twitch muscle fibers than Holstein one. Conversely, proteins detected from the Holstein sample included ankyrin repeat domain 2 and creatin kinase isomers. Given that creatin kinase isomers are related to the fast-twitch muscle, these results likely indicate that Holstein muscle tissue sampled for the current study contained more fast-twitch muscle fibers than Hanwoo beef.

• 한국수자원학회논문집
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• 제41권1호
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• pp.75-85
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• 2008

피라미는 국내 하천에서 서식하며 국지적인 회유를 하는 대표적인 어종이다. 피라미와 같이 작은 몸집의 국지회유성 어종은 서식처간의 이동이 생존을 위해 필수적이다. 그러나 어류 서식처간의 연결부가 배수암거(culvert)로 설치되어 있는 경우에는 평상시 빠른 유속이 발생되어 어류 이동에 많은 장애를 주고 있다. 외국에서는 배수암거의 설계유량과 유속기준이 홍수기간과 어류가 상류로 올라가는 기간별로 필요한 기능에 따라 제시되어 있어 홍수시에는 홍수소통기능, 평상시에는 어도기능이 발휘되도록 설계하고 있다. 암거식 어도의 설치는 암거의 어도기능을 향상시키는 방법 중 하나이다. 그러나 국내에서는 배수암거의 설계유량과 유속기준이 홍수소통기능에 대해서만 제시되어 있어 어도기능은 고려하지 못하고 있다. 본 연구에서는 옵셋배플 설치후 수리특성 및 어류이동 효과의 실험을 실험수로 내에서 일정간격의 유속단계별로 옵셋배플 설치전과 비교하며 분석하였다. 실험 결과 유속 1.2m/s 경우에서는 높이 5cm의 옵셋배플 설치 후 유속이 저감됨에 따라 피라미가 암거식 어도를 따라서 상류로 거슬러 올라가는 성공률, 즉 소상성공률이 최대 20% 개선됨을 확인하였다. 특히 직각으로 설치된 짧은 배플 구간이 피라미가 이동하는 주 이동경로로 나타났다. 그리고 FLOW-3D 모형을 이용한 3차원 수치해석을 수행하여 어도 설계시 수치모형실험의 활용가능성을 검토하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권7호
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• pp.942-948
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• 2005

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

• 대한전기학회논문지:시스템및제어부문D
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• 제53권4호
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• pp.277-285
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• 2004

We present a new hybrid stereo matching technique in terms of the co-operation of area-based stereo and feature-based stereo. The core of our technique is that feature matching is carried out by the reference of the disparity evaluated by area-based stereo. Since the reference of the disparity can significantly reduce the number of feature matching combinations, feature matching error can be drastically minimized. One requirement of the disparity to be referenced is that it should be reliable to be used in feature matching. To measure the reliability of the disparity, in this paper, we employ the self-consistency of the disunity Our suggested technique is applied to the detection of 3D line segments by 2D line matching using our hybrid stereo matching, which can be efficiently utilized in the generation of the rooftop model from urban imagery. We carry out the experiments on our hybrid stereo matching scheme. We generate synthetic images by photo-realistic simulation on Avenches data set of Ascona aerial images. Experimental results indicate that the extracted 3D line segments have an average error of 0.5m and verify our proposed scheme. In order to apply our method to the generation of 3D model in urban imagery, we carry out Preliminary experiments for rooftop generation. Since occlusions are occurred around the outlines of buildings, we experimentally suggested multi-image hybrid stereo system, based on the fusion of 3D line segments. In terms of the simple domain-specific 3D grouping scheme, we notice that an accurate 3D rooftop model can be generated. In this context, we expect that an extended 3D grouping scheme using our hybrid technique can be efficiently applied to the construction of 3D models with more general types of building rooftops.

• IMMUNE NETWORK
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• 제3권2호
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• pp.126-135
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• 2003

Background: One of the important factors in the prognosis of chronic hepatitis B patient is the degree of replication of hepatitis B virus (HBV). It has been known that HBV DNA polymerase plays the essential role in the replication of HBV. HBV DNA polymerase is composed of four domains, TP (Terminal protein), spacer, RT (Reverse transcriptase) and RNaseH. Among these domains, tyrosine, the $65^{th}$ residue of TP is an important residue in protein-priming reaction that initiates reverse transcription. If monoclonal antibody that recognizes around tyrosine residue were selected, it could be applied to further study of HBV replication. Methods: To produce TP-specific scFv (single-chain Fv) by phage display, mice were immunized using synthetic TP-peptide contains $57{\sim}80^{th}$ amino acid residues of TP domain. After isolation of mRNA of heavy-variable region ($V_H$) and light-chain variable region ($V_L$) from the spleen of the immunized mouse, DNA of $V_H$ and $V_L$ were obtained by RT-PCR and joined by a DNA linker encoding peptide (Gly4Ser)3 as a scFv DNA fragments. ScFv DNA fragments were cloned into a phagemid vector. scFv was expressed in E.coli TG1 as a fusion protein with E tag and phage gIII. To select the scFv that has specific affinity to TP-peptide from the phage-antibody library, we used two cycles of panning and colony lift assay. Results: The TP-peptide-specific scFv was isolated by selection process using TP-peptide as an antigen. Selected scFv had 30 kDa of protein size and its nucleotide sequences were analyzed. Indirect- and competitive-ELISA revealed that the selected scFv specifically recognized both TP-peptide and the HBV DNA polymerase. Conclusion: The scFv that recognizes the TP domain of the HBV DNA polymerase was isolated by phage display.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.315-315
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• 2017

In Japan, more than 80 % of soybean growing area is converted fields and excess water is one of the major problems in soybean production. For example, recent study (Yoshifuji et al., 2016) suggested that in the fields of shallow groundwater level (GWL) (< 1m depth), rising GWL even in a short period (e.g. 1 day) causes inhibition of soybean growth. Thus it becomes more and more important to predict GWL and soil moisture in detail. In addition to conventional surface drainage and underdrain, FOEAS (Farm Oriented Enhancing Aquatic System), which is expected to control GWL in fields adequately, has been developed recently. In this study we attempted to predict GWL and soil moisture condition at the converted field with FOEAS in Biwa lake reclamation area, Shiga prefecture, near the center of the main island of Japan. Two dimensional HYDRUS model (Simuinek et al., 1999) based on common Richards' equation, was used for the calculation of soil water movement. The calculation domain was considered to be 10 and 5 meter in horizontal and vertical direction, respectively, with two layers, i.e. 20cm-thick of plowed layer and underlying subsoil layer. The center of main underdrain (10 cm in diameter) was assumed to be 5 meter from the both ends of the domain and 10-60cm depth from the surface in accordance with the field experiment. The hydraulic parameters of the soil was estimated with the digital soil map in "Soil information web viewer" and Agricultural soil-profile physical properties database, Japan (SolphyJ) (Kato and Nishimura, 2016). Hourly rainfall depth and daily potential evapo-transpiration rate data were given as the upper boundary condition (B.C.). For the bottom B.C., constant upward flux, which meant the inflow flux to the field from outside, was given. Seepage face condition was employed for the surrounding of the underdrain. Initial condition was employed as GWL=60cm. Then we compared the simulated and observed results of volumetric water content at depth of 15cm and GWL. While the model described the variation of GWL well, it tended to overestimate the soil moisture through the growing period. Judging from the field condition, and observed data of soil moisture and GWL, consideration of soil structure (e.g. cracks and clods) in determination of soil hydraulic parameters at the plowed layer may improve the simulation results of soil moisture.

• Asian-Australasian Journal of Animal Sciences
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• 제29권1호
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• pp.126-133
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• 2016

A gene from Actinomyces sp. Korean native goat (KNG) 40 that encodes an endo-${\beta}$-1,4-glucanase, EG1, was cloned and expressed in Escherichia coli (E. coli) $DH5{\alpha}$. Recombinant plasmid DNA from a positive clone with a 3.2 kb insert hydrolyzing carboxyl methyl-cellulose (CMC) was designated as pDS3. The entire nucleotide sequence was determined, and an open-reading frame (ORF) was deduced. The ORF encodes a polypeptide of 684 amino acids. The recombinant EG1 produced in E. coli $DH5{\alpha}$ harboring pDS3 was purified in one step using affinity chromatography on crystalline cellulose and characterized. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis/zymogram analysis of the purified enzyme revealed two protein bands of 57.1 and 54.1 kDa. The amino terminal sequences of these two bands matched those of the deduced ones, starting from residue 166 and 208, respectively. Putative signal sequences, a Shine.Dalgarno-type ribosomal binding site, and promoter sequences related to the consensus sequences were deduced. EG1 has a typical tripartite structure of cellulase, a catalytic domain, a serine-rich linker region, and a cellulose-binding domain. The optimal temperature for the activity of the purified enzyme was $55^{\circ}C$, but it retained over 90% of maximum activity in a broad temperature range ($40^{\circ}C$ to $60^{\circ}C$). The optimal pH for the enzyme activity was 6.0. Kinetic parameters, $K_m$ and $V_{max}$ of rEG1 were 0.39% CMC and 143 U/mg, respectively.