• Horticultural Science & Technology
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• v.29 no.2
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• pp.123-129
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• 2011

To identify unintended vertical gene-transfer rates from the developed transgenic plants, rapid and unequivocal techniques are needed to identify event-specific markers based on flanking sequences around the transgene and to distinguish zygosity such as homo- and hetero-zygosity. To facilitate evaluation of zygosity, a polymerase chain reaction technique was used to analyze a transgenic pepper line B20 (homozygote), P915 wild type (null zygote), and their F1 hybrids, which were used as transgene contaminated plants. First, we sequenced the 3'-flanking region of the T-DNA (1,277 bp) in the transgenic pepper event B20. Based on sequence information for the 3'- and 5'-flanking region of T-DNA provided in a previous study, a primer pair was designed to amplify full length T-DNA in B20. We successfully amplified the full length T-DNA containing 986 bp from the flanking regions of B20. In addition, a 1,040 bp PCR product, which was where the T-DNA was inserted, was amplified from P915. Finally, both full length T-DNA and the 1,040 bp fragment were simultaneously amplified in the F1 hybrids; P915 ${\times}$ B20, Pungchon ${\times}$ B20, Gumtap ${\times}$ B20. In the present study, we were able to identify zygosity among homozygous transgenic event B20, its wild type P915, and hemizygous F1 hybrids. Therefore, this novel zygosity identification technique, which is based on PCR, can be effectively used to examine gene flow for transgenic pepper event B20.

• The Journal of the Acoustical Society of Korea
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• v.19 no.3
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• pp.51-56
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• 2000

Is this paper, we studied on the automatic speech control system in real-time windows environment using voice recognition. The applied reference pattern is the variable DMS model which is proposed to fasten execution speed and the one-stage DP algorithm using this model is used for recognition algorithm. The recognition vocabulary set is composed of control command words which are frequently used in windows environment. In this paper, an automatic speech period detection algorithm which is for on-line voice processing in windows environment is implemented. The variable DMS model which applies variable number of section in consideration of duration of the input signal is proposed. Sometimes, unnecessary recognition target word are generated. therefore model is reconstructed in on-line to handle this efficiently. The Perceptual Linear Predictive analysis method which generate feature vector from extracted feature of voice is applied. According to the experiment result, but recognition speech is fastened in the proposed model because of small loud of calculation. The multi-speaker-independent recognition rate and the multi-speaker-dependent recognition rate is 99.08% and 99.39% respectively. In the noisy environment the recognition rate is 96.25%.

• Journal of Radiation Industry
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• v.7 no.1
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• pp.69-74
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• 2013

This study examined radiation detection characteristics of spices (cumin, caper and turmeric), other small fruits (hut-gae berry and boxthorn), and pollen extract product. Each samples were irradiated at dose of 1, 5, and 10 kGy and analyzed by the thermoluminescence (TL) and electron spin resonance (ESR) methods. To compare between non-irradiated and irradiated food, all samples were irradiated with $^{60}Co$ gamma-ray source. In TL analysis, most of samples could be applied to detect irradiated foods because of showing TL ratio above 0.1. The glow curves examined by TL method were estimated in the range of $150{\sim}250^{\circ}C$ in irradiated samples. In ESR measurements, the intensity of ESR signal (single-line) increased as the increase of irradiation dose. In particular, the specific ESR signals of irradiation-induced radical were detected in hut-gae berry and pollen extract product. As a results, it is considered that TL and ESR methods can be used to detect both hut-gae berry and pollen extract product. But cumin, caper, turmeric and boxthorn irradiated with gamma ray could be detected only by TL method. It is concluded that TL and ESR methods are suitable for detection of irradiated samples and a combined method is recommendable for enhancing the reliability of detection results.

• Journal of the Korean Society for Nondestructive Testing
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• v.25 no.2
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• pp.95-102
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• 2005

There are many tube and pipeline in nuclear power plant under high temperature and high pressure. Erosion and corrosion defects were expected on these tube and pipe-line by environmental and mechanical factors. These erosion and corrosion defects ran be evaluated by ultrasonic technique. In these study, Scanning Laser Source(SLS) technique was applied to detect defect and construct image. This technique also makes detection possible on rough and curved surfaces such as tube and pipe-line by scanning. Conventional ultrasonic scanning technique requires immersion of specimen or water jet for transferring ultrasonic wave between transducer and specimen. However, this SLS technique does not need contacting and couplant to generate surface wave and to get flaw images. Therefore, this SLS technique has several advantages, for complicated production inspection, non-contact, remote from specimen, and high resolution. In this study, SLS images were obtained with various conditions of generation laser ultrasound and receiving in order to enhance detectability of flaws on the tube. Stress corrosion cracks were produced on tube and images of stress corrosion cracks were constructed by using SLS technique.

• Quality Improvement in Health Care
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• v.4 no.1
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• pp.36-49
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• 1997

Background : Hypertension is one of the most important risk factors of the cerebrovascular accident and coronary artery disease which are the major causes of mortality in Korea. In Korea, the quality of care provided by office-based physicians has not been evaluated formally. The purpose of this study is to assess the quality of hypertension management of office-based physicians. Method : Self-administered questionnaires were mailed to the office-based physicians with the speciality of internal medicine, general surgery, family medicine, and general practitioners. Among 2,045 physicians, 981 doctors(48.0%) replied the questionnaires. Contents of questionnaires were based on the recommendation from the JNC-V report(the Fifth Report of the Joint National Committee on Detection, Evaluation, and Treatment of High Blood Pressure), and included the criteria of diagnosis, treatment, follow-up interval, and other characteristics of physicians(age, sex, type of speciality, and location of practice). Results : Eighty four percent of the office-based physicians made diagnosis of hypertension with less than 3 times of blood pressure measurements. The performance rate of required examination for hypertensives was very low in most items. Rate of fundoscopic examination is the lowest one among them(5.9%). The performance rate of laboratory examination was also low in most items. Internists tended to order more frequent laboratory examinations than any other type of physicians. Only 11.4% of the physicians did appropriate treatments for the mild hypertension case. The antihypertensives selected by the physicians as a first line drug were in the order of beta blocker(26.4%), calcium channel blocker(23.4%), diuretics(23.1%), ACE inhibitors(14.3%). The visit interval for established hypertensives was very short. Proportion of physicians with follow-up interval longer than 4 weeks was only 4.3%. Conclusions : The overall quality of hypertension management of office-based physicians in Korea is very problematic in many aspects. So further investigations to find out the reasons of low quality arid quality of care should be initiated.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.149-152
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• 2005

A new methodology to detect forest fire burnt scars at near real time using MODIS (Moderate-resolution Imaging Spectroradiometer) data is presented here with a goal of introducing a new and improved capability to detect forest fire burnt scars in Thailand. This new technology is expected to increase the efficiency and effectiveness of the forest fire tackling resources distribution and management of the country. Using MODIS data in burnt scars detection has two major advantages - high availability of data and high resolution per performance ratio. Results prove the near real time algorithm suitable and working well in order to monitor the forest fire dynamic movement. The algorithm is based on the threshold separated linear equation of burnt and un-burnt. A ground truth experiment confirms the burnt and un-burnt? areas characteristics (temperature and NDVI). A threshold line on a scatter plot of Band I and Band 2 is determined to separate the burnt from un-burnt pixels. The different threshold values of NDVI and temperature use to identify pixels' anomaly, abnormal low NDVI and high temperature. The overlay (superimpose) method is used to verify burnt pixels. Since forest fire is a dynamic phenomenon, MODIS burnt scars information is suiting well to fill in the missing temporal information of LANDSAT for the forest fire control managing strategy in Thailand. This study was conducted in the Huai-Kha-Kaeng (HKK) Wildlife Sanctuary, Thailand

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.253.2-253.2
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• 2014

Micro hollow cathode discharges (MHCDs) are high-pressure, non-equilibrium discharges. Those MHCDs are useful to produce an excimer radiation. A major advantage of excimer sources is their high internal efficiency which may reach values up to 40% when operated under optimum conditions. To produce strong excimer radiation, the optimisation of the discharge conditions however needs a detailed knowledge of the properties of the discharge plasma itself. The electron density and temperature influence the excitation as well as plasma chemistry reactions and the gas temperature plays a major role as a significant energy loss process limiting efficiency of excimer radiation. Most of the recent spectroscopic investigations are focusing on the ultraviolet or vacuum ultraviolet range for direct detection of the excimer. In our experiments we have concentrated on investigating the micro hollow cathodes from the near UV to the near infrared (300~850 nm) to measure the basic plasma parameters using standard plasma diagnostic techniques such as stark broadening for electron density and the relative line intensity method for electron temperature. Finally, the neutral gas temperature was measured by means of the vibrational rotational structures of the second positive system of nitrogen.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.9B
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• pp.1259-1265
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• 2010

Recently, on-line social network has been increasing according to development of internet. The most representative service is blog. A Blog is a type of personal web site, usually maintained by an individual with regular entries of commentary. These blogs are related to each other, and it is called Blog Network in this paper. In a blog network, posts in a blog can be diffused to other blogs. Analyzing information diffusion in a blog world is a very useful research issue, which can be used for predicting information diffusion, abnormally detection, marketing, and revitalizing the blog world. Existing studies on network analysis have no consideration for the passage of time and these approaches can only measure network activity for a node by the number of direct connections that a given node has. As one solution, this paper suggests the new method of measuring the blog network activity using logistic curve model and Cosine-similarity in key words by the Map/Reduce programming model.

• Proceedings of the KIEE Conference
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• 1998.07c
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• pp.1166-1169
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• 1998

The Load characteristic of electric railway requires the power demand of the high capacity which amplitude is spacial-temporally fluctuated due to frequent starting and stopping with large tractive force. The conventional electric railway mainly consists of the resistance controlled and the thyristor controlled locomotives, are compensated for their bad characteristics of the power factor$(70\sim80%)$ with installation of another capacitor improving power factor at the substation. Since 1994, VVVF train car with good characteristics of power factor(100%) have been introduced and operated in Kwa-Chon Line. From the present technical tendency, it is judged that introduction of the locomotive with various controlled methods is necessary. The protective equipments installed at the substation are complicated and various aspects to detect faults and reduce their extension, so the universal countermeasures are required. Specially in the case of the fault occurrence it is difficult to calculate the fault location because of the change in the contactline constant according to modifying the characteristics of the contactline (the dualized catenary wire and extension, etc), so much time is required for the detection of fault location. In BT-fed method distance-relays and fault-locators are not installed, we have so many difficulties in the quick accident recovery.

• The Korean Journal of Physiology and Pharmacology
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• v.10 no.6
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• pp.349-354
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• 2006

The aromatic hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates many of the biological and toxicological effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, dioxin) and related chemicals. The application of recombinant reporter plasmid such as the firefly luciferase gene has proven to be a very effective method to detect these chemicals. The bioassay system, CALUX, is sensitive in directly detecting AhR-agonists from a variety of environmental and biologic materials. However, responses of the AhR-dependent bioassays are dependent on the cell types used. Thus, we developed a sensitive bioassay using the recombinant mouse hepatoma cell (Hepa1c1c7) for the determination of dioxins. The recombinant cell line was stably transfected with firefly luciferase reporter gene (pGudLuc1.1). The transfected cells showed the highest induction of luciferase activity at 4.5 hr and a decrease beyond this time point. The system showed the highest sensitivity of detection ever reported. Upon TCDD exposure cells showed 2 fold increase at 10 pM and 7 fold increase at 100 pM, respectively. The passage number after the transfection played an important role in the sensitivity. The increase of passage number tended to increase the sensitivity of the cells up to 15. The media without phenol red showed a higher induction rate than with phenol red, suggesting the preferable use of phenol red-free media for the bioassay. Since each of the assays has unique characteristics that make them suitable for some screening applications and not others, development of sensitive bioanalytical methods based on a variety of cellular systems in a key to the successful determination of dioxins. The bioassay system developed in this study will contribute to further development of successful screening the AhR agonists among the environmental mixture. In addition, the rapid and sensitive nature of this cellular system can be applied as a valuable tool to screen the dioxin-like moieties among the prodrugs at the initial stage, thereby expediting the new drug discovery.