• Journal of Plant Biotechnology
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• 제37권4호
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• pp.379-387
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• 2010

Oilseed rape (Brassica napus L.) is an important crop due to its high oil content in the seed. Recently, the demand for the improvement of crop for biodisel energy source is increased as oil prices in the world has increased dramatically. Until now, oilseed rape breeding was carried out by cross-hybridization between different varieties and related germplasms. However, like as many other crops, the application of tissue culture and gene transformation systems has been introduced into oilseed rape breeding program including the development of transgenic canola plants. In this study, we reviewed a history of tissue culture and genetic transformation research in oilseed rape plants and indicated some important aspects for the production of transgenic oilseed rape plants.

• 한국유기농업학회지
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• 제23권2호
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• pp.207-231
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• 2015

This study evaluate examines the efficiency and the improvement measurement of Oilseed crops (Sesame and Perilla). For this purpose, In the first stage, this study analyzes the current conditions of oilseed industry. In the second stage, this study evaluates the efficiency and super-efficiency of environmentally-friendly agricultural product producers. The result of this study show that: (1) Changes in annual wholesale price of Sesame and Perilla; (2) An efficiency and ranking of environmentally-friendly product producers; (3) The solutions and improvement measurements for inefficient producers.

• 한국유기농업학회지
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• 제23권2호
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• pp.159-183
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• 2015

This study examines the management performance of Oilseed crops (Sesame and Perilla). For this purpose, In the first stage, This study analyzes the current status of sesame and perilla industry. In the second stage, This study examines the management performance of environmental friendly agricultural products (Sesame and perilla). The result of this study show that : (1) Changes in annual wholesale price of Sesame and Perilla ; (2) Management performance of environmental friendly products (Sesame and perilla) ; (3) Feature comparison of productivity of oilseed crops.

• Journal of Ecology and Environment
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• 제36권3호
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• pp.167-172
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• 2013

A Two-year field experiment was carried out in 2009 and 2010 to evaluate the effects of different densities of wild oat and nitrogen (N) rates on oilseed rape yield and yield components. Experimental design was split plot with three replications. Increased nitrogen rates up to 150 kg N/ha caused an increase in oilseed rape grain yield in weed-free plots (4.26 t/ha; 1t = $10^3kg$), while even at the lowest density of wild oat (15 $plants/m^2$), increasing N fertilizer only up to 50 kg/ha led to a significant increase in grain yield. Wild oat growth was favored by high levels of N. Intraspecific competition reduced biomass, 100-seed weight and number of tillers of wild oat. Both lower N rate and intraspecific competition had negative effect on wild oat growth but the weed was still able to produce a considerable number of tillers and vigorous seeds. It showed that wild oat possess a notable adaptive plasticity and can allocate a greater proportion of its resource toward reproductive organs even under resource shortage conditions and, thus, it may increase the competitive ability of the weed over the crop.Our results suggest that effective weed management should be done to prevent wild oat seed production in crops preceding oilseed rape as well as to control the weed at early season in oilseed rape fields.

• 한국작물학회지
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• 제36권5호
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• pp.445-458
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• 1991

유지작물의 안전 생산과 품질향상을 위해 지금까지 유지작물에 발생되는 주요 기상재해에 대한 국내외 문헌을 종합 고찰하였다 유지작물에 발생되는 주요 기상재해는 타작물에서와 같이 냉해나 한해와 같은 온도에 의한 장해, 습해나 도복과 같은 풍수해, 그리고 기타재해 등 각종 재해가 발생되고 있는 것으로 보고되있다. 그러나 유지작물은 국내외를 막론하고 주요작물에 비해 비중이 낮고 재배면적도 적어 비중이 낮게 취급된 관계로 각종 재해에 대한 정확한 피해정도나 재해경감 대책이 밝혀지지 많은 부분이 많은 것으로 고찰되었다. 지금까지 조사 보고된 결과들 중에서는 참깨와 땅콩에 대한 결과들이 많고 그 중에서도 침관수해, 한충해, 도복피해 등 풍수해에 대한 연구결과가 많으며 이 같은 재해들은 피해정도도 매우 큰 것으로 보고되어 심한 경우는 90%이상의 수량감소를 초래하는 것으로 보고하였다. 이와 같은 재해에 대한 피서를 최소화하기 위해서는 우선 품종개량 면에서 각종 재해에 대한 내성을 가진 품종이 개발되어야 할 것이며, 재배기술적인 측면에서도 재해를 분산시킬 수 있는 방법이나 비닐멀칭재배법 등과 같이 기상재해의 사전예방 기술도 적용되어야 할 것으로 고찰되었다. 그러나 최근의 농산물유입 개방화에 따른 다품목 소량재배 추세와 다양한 식품의 안정적 생산공급 측면에서 점차 비중이 높아가고 있는 유서 작물의 안전생산과 품질향상을 위해서는 품종개량과 재배기술 면에서 보다 적극적인 기상재해 경감대책 연구와 노력이 필요한 것으로 전망되었다.

• 한국작물학회지
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• 제47권
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• pp.175-185
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• 2002

Rapeseed(Brassica napus L.) is an important oil crop as a vegetable oil, concentrated feed and industrial materials. The name "canola" was registered in 1979 by the Western Canadian Oilseed Crushers Association to describe "double-low" varieties. Double low indicates that the processed oil contains less than 2% erucic-acid and the meal less than 3mg/g of glucosinolates. Today annual worldwide production of rapeseed is approximately 35 million tons on 24 million hectares. China accounts for 33% of the world production and the European Economic Community for nearly 32%. Canola ranks 3rd in production among the world's oilseed crops following soybeans, sunflowers, peanuts and cottonseed. The recent advances in genomics and in gene function studies has allowed us to understand the detailed genetic basis of many complex traits, such as flowering time, height, and disease resistance. The manipulation of seed oil content via transgene insertion has been one of the earliest successful applications of modern biotechnology in agriculture. For example, the first transgenic crop with a modified seed composition to be approved for unrestricted commercial cultivation in the US was a lauric oil, rape-seed, grown in 1995. There were also some significant early successes, mostly notably the achievement of 40% to 60% lauric acid content in rapeseed oil, which normally accumulates little or no lauric acid. The name "$\textrm{Laurical}^{TM}$" was registered in 1995 by Calgene Inc. Nevertheless, attempts to achieve high levels of other novel fatty acids in seed oils have met with much less success and there have been several reports that the presence of novel fatty acids in transgenic plants can sometimes lead to the induction of catabolic pathways which break down the novel fatty acid, i.e. the plant recognizes the "strange" fatty acid and, far from tolerating it, may even actively eliminate it from the seed oil. It is likely that, in the future, transgenic oil crops and newly domesticated oil crops will both be developed in order to provide the increased amount and diversity of oils which will be required for both edible and industrial use. It is important that we recognize that both approaches have both positive and negative points. It will be a combination of these two strategies that is most likely to supply the increasing demands for plant oils in the 21st century and beyond.ant oils in the 21st century and beyond.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1991년도 추계 학술대회지
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• pp.20-21
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• 1991

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.21-21
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• 2022

• BMB Reports
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• 제40권2호
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• pp.247-260
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• 2007

Cinnamate 4-hydroxylase (C4H) is a key enzyme of phenylpropanoid pathway, which synthesizes numerous secondary metabolites to participate in development and adaption. Two C4H isoforms, the 2192-bp BnC4H-1 and 2108-bp BnC4H-2, were cloned from oilseed rape (Brassica napus). They both have two introns and a 1518-bp open reading frame encoding a 505-amino-acid polypeptide. BnC4H-1 is 57.73 kDa with an isoelectric point of 9.11, while 57.75 kDa and 9.13 for BnC4H-2. They share only 80.6% identities on nucleotide level but 96.6% identities and 98.4% positives on protein level. Showing highest homologies to Arabidopsis thaliana C4H, they possess a conserved p450 domain and all P450-featured motifs, and are identical to typical C4Hs at substrate-recognition sites and active site residues. They are most probably associated with endoplasmic reticulum by one or both of the N- and C-terminal transmembrane helices. Phosphorylation may be a necessary post-translational modification. Their secondary structures are dominated by alpha helices and random coils. Most helices locate in the central region, while extended strands mainly distribute before and after this region. Southern blot indicated about 9 or more C4H paralogs in B. napus. In hypocotyl, cotyledon, stem, flower, bud, young- and middle-stage seed, they are co-dominantly expressed. In root and old seed, BnC4H-2 is dominant over BnC4H-1, with a reverse trend in leaf and pericarp. Paralogous C4H numbers in Brassicaceae genomes and possible roles of conserved motifs in 5' UTR and the 2nd intron are discussed.

• 한국작물학회지
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• 제55권2호
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• pp.151-158
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• 2010

지금까지 양구슬냉이의 유전정보는 거의 연구되지 않았으므로 우리는 양구슬냉이의 잎으로부터 cDNA library를 제작하고 발현유전자의 종류와 기능별 분류를 조사하였다. 그 결과를 요약하면 다음과 같다. 1. cDNA library에서 1334개 의 클론들을 얻었고 삽입된 단편들의 염기서열의 평균길이는 736bp였다. 우리는 1269개의 high-quality expressed sequence tags (ESTs) 서열을 얻었다. 이러한 EST의 클러스터 분석결과 고유 염기서열(unigene)을 가진 유전자의 수는 851개를 나타냈다. 2. Unigene 476개는 GeneBank에 기능이 알려진 유전자들과 고도의 상동성을 나타내었다. 다른 375개의 unigene들은 기능이 알려지지 않은 것들이었다. 나머지 63개는 NCBI데이터베이스에 어떤 유전자와도 상동성을 보이지 않았고 이러한 유전자들은 아마도 양구슬냉이의 잎에서 발현되는 새로운 유전자일 것으로 보인다. 3. 데이터베이스에서 상동성을 나타낸 EST들을 기능별 주석에 따라서 17개의 카테고리로 분류하였다. 대표적으로 가장 분포도가 높은 카테고리는 결합 기능 또는 보조인자 요구의 단백질(27%), 대사(11%), 세포 소기관 위치(11%), 세포수송과 수송기관 그리고 수송 경로(7%), 에너지(6%), 대사와 단백질 기능의 조절(6%) 등이 있다. 이러한 우리의 연구 결과는 양구슬냉이의 유용한 유전적 자원과 전반적인 mRNA 발현 정보를 제공해 줌으로써 대체 에너지 작물로 떠오르는 양구슬냉이의 다양한 분자적 연구에 기여할 것으로 사료된다.