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Powdery Mildew Resistance Phenotype Test & Genotype Test in C. moschata

  • Jong-Gyu Park
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.290-290
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    • 2022
  • Powdery mildew is known to be one of the serious diseases in C. moschata cultivation. Plants infected with powdery mildew cause damage to cultivation areas such as occurrence of deformity fruit and decrease in quantity. also, it has been reported that many farms have difficulties in controlling powdery mildew due to the outbreak under various conditions throughout the year. Therefore, this study intends to perform a phenotype test and a genotype test for C. moschata 60 lines grown in Jenong S&T. Podospareaxanthii, known as a pathogen that causes powder mildew disease in pumpkins in Korea, was collected and used as an inoculation source, phenotype test was performed by examining the infection area rate(%) of powdery mildew disease that occurred in leaves 25 days after inoculation. It was determined that 0% of the infection area rate was in the first stage, 1 to 5% in the second stage, 6 to 15% in the third stage, 16 to 30% in the fourth stage, and 31% or more in the fifth stage, The first and second stages were judged as resistance, the third as moderate resistance, and the fourth and fifth stages as sensitivity. As a result of the phenotype test, it was confirmed that the resistance was 21 points, moderate resistance was 14 points, and sensitivity was 25 points. After searching for the genes related to powdery mildew resistance resistance, pm-0, CmbHLH87, and LOC111453072, 21 points of resistance and 9 points of moderate resistance identified through phenotype tests were identified through gel electrophoresis after polymerase chain reaction(PCR) using 5 primers related to 3 genes. As a result of genotype testing of a total 30 points, the CmbHLH87 and LOC111453072 gene were found to be resistant bands in all points, PMR1 was identified as 20 points for resistance, 4 points for moderate resistance, and 6 points for sensitivity, PMR2 was not identified in the entire band, and PMR5 was identified as 18 point for resistance, 3 points for moderate resistance, and 9 points for sensitivity. As a result, when comparing the phenotype test results and genotype test results, CmbHLH87 and LOC111453072 genes was 100% consistent in resistance and moderate resistance, PMR1 was 95.2% in resistance, 44.4% in moderate resistance, and PMR5 was 90% in resistance and 33.3% in moderate resistance, PMR2 was not consistent in resistance and moderate resistance. Therefore, it is expected that more accurate PMR test will be possible by using molecular markers(PMR1, PMR5) and by developing CmbHLH87 and LOC111453072 gene-related molecular markers.

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A Study on the Design of Standard Code for Hazardous and Noxious Substance Accidents at Sea (해상 HNS 사고 표준코드 설계에 관한 연구)

  • Ha, Min-Jae;Jang, Ha-Lyong;Yun, Jong-Hwui;Lee, Moonjin;Lee, Eun-Bang
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.22 no.2
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    • pp.228-232
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    • 2016
  • As the quantity of HNS sea trasport and the number of HNS accidents at sea are increasing recently, the importance of HNS management is emphasized so that we try to develop marine accident case standard code for making HNS accidents at sea databased systemically in this study. First and foremost, we draw the related requisites of essential accident reports along with internal and external decrees and established statistics of classified items for conducting study, and we referred to analogous standard codes obtained from developed countries in order to research code design. Code design is set like 'Accident occurrence ${\rightarrow}$ The initial accident information ${\rightarrow}$ Accident response ${\rightarrow}$ Accident investigation' in accordance with the general flow of marine HNS accidents of in which the accident information is input and queried. We classified initial accident information into the items of five categories and constructed "Preliminary Information Code(P.I.C.)". In addition we constructed accident response in two categories and accident investigation in three categories that get possible after the accident occurrence as called "Full Information(F.I.C.)", including the P.I.C. It is represented in 3 kinds of steps on each topic by departmentalizing the classified majority as classified middle class and classified minority. As a result of coding marine HNS accident and of the code to a typical example of marine HNS accident, HNS accident was ascertained to be represented sufficiently well. We expect that it is feasible to predict possible trouble or accident henceforward by applying code, and also consider that it is valuable to the preparedness, response and restoration in relation to HNS accidents at sea by managing systemically the data of marine HNS accidents which will occur in the future.

Quantitative Analysis of the Structure and Dynamics of Benthic Marine Algal Communities at the Southern Coast of Korea 1. Yonhwa-do, near Chungmu (한국 남해안 동부 해역 해조 군집 구조 및 변동의 정량적 분석 1. 연화도)

  • SOHN Chul Hyun;LEE In Kyu;KANG Jae Won
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.3
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    • pp.265-273
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    • 1986
  • The marine algal communities of Yonhwa-do at the southern coast of Korea were investigated in aspects to quantitative analysis of their structure and dynamics. As a result, 114 species, including 2 blue-green, 69 red, 25 brown and 18 green algae, were identified. The R/P value(2.8) is higher than that of other regions of the southern coast of Korea. Sheet and filamentous groups of the functional groups of algae showed higher composition rate in spring and summer than in autumn and winter, but coarsely branched and thick leathery groups showed an opposite tendency. Cluster analysis based on species presence suggested the existence of 5 distinct groups in spring, but 3 groups in the other seasons. The species exhibiting important value above 20 were as follows: Porphyra suborbiculata, Corallina pilulifera, Hizikia fusiforme, Sargassum thunbergii, Undaria pinnatifida, Ishige okamurai, Chondria crassicaulis, and crustose coralline algae. The coverage of algal communities composed only of crustose coralline algal complex was higher than that of other algal complex, but occurrence frequency of these two communities was indifferent. It is suggested that the occurrence in high quantity of crustose coralline algae from this area is related to grazing by herbivorous animals.

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The Effects of Geological and Topographical Features on Landslide and Land-creep (지질(地質)과 지형(地形)이 산사태(山沙汰) 및 땅밀림에 미치는 영향(影響))

  • Jau, Jae-Gyu;Park, Sang-Jun;Son, Doo-Sik;Joo, Sung-Hyun
    • Journal of Korean Society of Forest Science
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    • v.89 no.3
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    • pp.323-334
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    • 2000
  • This study was carried out to investigate the effects of geological and topographical features on landslide and land-creep at the twenty four surveyed sites of Kyungpook province. According to the results obtained, it was concluded that continuous heavy rainfall was one of the primary factors to occur landslide and land-creep. Most of the landslides occurred in the past were concentrated in the granite and granitic gneiss zones, while land-creeps were mainly occurred in the mud-stone zones. Therefore, it was thought that the physical properties such as soil texture, solid phase, moisture contents, density, hardness and porosity rate of weathered granite and granitic gneiss could affect the occurrence of landslide and land-creep. Due to the holding of sand contents in the upper soil layers of weathered granite and granitic gneiss, rainfall could infiltrate into the soil easily. While lower soil layers contained much quantity of clay and silt contents, those soils saturated with rainfall cause to lose viscosity and shear strength. Therefore, it was seemed that landslide was occurred more easily and the saturation of those soils was made much easily by bed rocks under those soils. Landslide and land-creep are slided into lower place by gravitation and slope degree factors. Therefore, prediction of landslide occurrence is very difficult because landslide is occurred abruptly, and physical properties of the soil have to be understood and checking the existence of bed rocks under the soils is not easy, on the other hand, land-creep is progressed very slowly. Therefore, it was suggested that in a degree creeping could be protected by removing of several causing factors.

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Influence of Nutritional Supplementation to the Substrate on Vegetative and Reproductive Growth of Winter Mushroom, Flammulina velutipes (Curt. ex Fr.) Sing. and Chemical Changes of the substrates Produced during Growth of the Fungus (톱밥 배지(培地)에 대(對)한 영양첨가(營養添加)가 팽이버섯의 생장(生長)및 배지(培地)의 화학적(化學的) 성분(成分) 변화(變化)에 미치는 영향(影響))

  • Chang, Hak-Gil
    • The Korean Journal of Mycology
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    • v.4 no.1
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    • pp.31-44
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    • 1976
  • The studies were carried out to examine the effects of supplementation of nutritional substances and physical conditions in substrate on the mycelial growth and yield of fresh sporophores of winter mushroom, Flammulina velutipes(Curt. ex Fr.) Sing. and to obtain further informations on the nutritional requirements of the fungus with reference to improvement of substrate through [analysis of chemical composition of the substrates during the cultivation period. The results obtained are summarized as follows: 1. The best yield of fresh sporophores, 84.4 g per 280 g substrate in a bottle, was obtained from the mixture of poplar sawdust 10 and rice bran 3 by volume when Flammulina velutipes was cultivated on the poplar sawdust supplemented by rice bran, wheat bran, cattle manure and various combinations of these materials as nutrient sources. The substrates of poplar sawdust 10 plus rice bran 3 and 2 or wheat bran 3 with a higher yield of fresh sporophores showed a comparatively higher content of total nitrogen. total sugar, and potassium. 2. The mycelial growth of the fungus was compared on the substrates of poplar sawdust supplemented by the several nutrient sources and poplar sawdust alone. The fastest linear growth occurred on substrates of poplar sawdust alone and poplar sawdust plus cattle manure deficient in sugar and nitrogen sources, but mycelial density was more sparse on the substrates. Also, growth in a solution extracted from these substrates was very meager. 3. In the substrates which varied with bulk density and moisture content optimum bulk density and moisture content for mycelial growth was 0.2g/cc and 72% on a dry weight basis, respectively, but the highest yield of fresh sporophores was obtained at the bulk density of 0.3g/cc and moisture content of 67%. 4. By increasing the ratio of rice bran in poplar sawdust the loss of total weight and ash, content at each stage was increased, and during the cultivation period of 75 days, loss of total weight of the substrates at inoculation was 17.8 to 28.8% and ash content increased about 12%. 5. 11 to 14% of the cellulose and 3 to 4% of the lignin content per original substrate were decreased without a great difference depending of the mixing ratio of rice bran. The soluble glucose concentration in the substrates was increased during the same period. 6. In the process of vegetative and reproductive growth of the fungus upon the substrates, the total nitrogen was increased in quantity per dry weight of sample but was reduced in absolute quantity to a minute extent. There is no great changes in content of organic nitrogen including amino acid nitrogen, and hydrolysable ammonium nitrogen during the vegetative growth period, but occurrence of sporophores resulted in a decrease in the nitrogen content of these forms. On the one hand, by an increase of additive amounts of rice bran, nitrogen contents of these forms were higher and the reduction range during the reproductive growth period became wider. 7. Mycelial growth of the fungus was accelerated in various liquid media supplemented with organic nitrogen sources such as peptone and yeast extract in comparison with addition of inorganic nitrogen sources. Furthermore, mycelial growth was mere vigorous in the media with higher content of organic nitrogen sources.

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Population Characteristics of Echinosophora koreensis (Nakai) Nakai, a Endemic Plants in Korea (한반도 특산식물 개느삼의 개체군 특성)

  • Kim, Sodam;Moon, Ae-Ra;An, Jong-Bin;Jung, Ji-Young;Park, Wan-Geun;Son, Sungwon
    • Korean Journal of Environment and Ecology
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    • v.34 no.1
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    • pp.18-26
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    • 2020
  • Echinosophora koreensis (Nakai) Nakai an endemic plant with limited distribution in some parts of the Korean Peninsula, is designated as "Endangered" species on the IUCN Red List. The species is under the threat of deterioration in nature due to various environmental changes causing loss of natural habitats. We investigated the distribution pattern and population structure of E. koreensis to serve as a basic reference for identifying the dynamics and persistence of natural populations of this species in the future. To determine the characteristics of the E. koreensis population, we laid two to three large plots sized 20m×30m on the ridgeline as the reference in Yanggu, Chuncheon, and Hongcheon and laid four to seven small quadrats sized 1m×1m on the transect. A total of 530 plots were laid, 10 each at 2m interval, to measure the number of the stems, the number of fruiting, height, and other factors. The collected distribution information was compared with the existing IUCN assessed categories by applying IUCN Red List ver 3.1 Criteria B. The average population density of the three surveyed areas was 3.47 stem/㎡; the density of each area was 3.95 stems/㎡ in Yanggu, 3.37 stems/㎡ in Chuncheon, and 2.87 stems/㎡ in Hongcheon. The number of fruiting per stem was 0.0038, indicating only 7 fruits out of 1,837 stems and that vegetative reproduction is likely to be more dominant than sexual reproduction. The distribution tendency of population density in the small plots in three surveyed areas showed that the density of E. koreensis tended to decrease toward the center of the forest with low crown openness and around the mountain ridge with high crown openness. It indicated that the quantity of light could be a very important factor for the growth of individuals of this species. The analysis of the heights of individuals present in the surveyed plots showed a range of 2.3 ~ 68.5cm and an average of 20.1cm. An analysis on the stage-structure of the E. koreensis population based on the sieve height found that the appearance frequency decreased with increasing height above 15 ~ 20cm and that the percentage of individuals lower than 10cm, which were presumed to be seedlings, was severely low, indicating a necessity of study on the population sustainability based on the monitored data. The occupied area calculated with the collected species distribution information was 200㎢, indicating the EN category according to the IUCN Red List Reference B.

Field research of cultivation technique for stable production of common mushroom(Agaricus bisporus) (양송이 안정생산을 위한 생산기술 현장연구)

  • Jhune, Chang-Sung;Jang, Kab-Yeul;Jeong, Jong-Cheon;Lee, Chan-Jung;Kong, Won-Sik;Yoo, Young-Bok
    • Journal of Mushroom
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    • v.8 no.3
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    • pp.122-130
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    • 2010
  • Common mushroom production per area has been decreased and are up to less than 50% of the 1980 production. To determine the main reasons for the decrement, we performed this study. Two main reasons, which are mushroom disease and the low compost quality because of mechanized compost making, were assessed. In mechanized mushroom farms, nitrogen concentration in compost was lower than recommended and total compost quantity was about 100-150 $kg/3.3m^2$, which was also lower than usual. Our study revealed that higher nitrogen concentration (about 1.5%) in compost gave better production. Also, use of large amount of compost appeared to increase the mushroom production, although more insects and disease problems were observed. The relationships between the presences of microorganisms and occurrence of diseases were assessed by monitoring the microorganism densities near the mushroom farms. Higher number of microorganisms were observed near the mushroom farm area, compared to control region, Daechon beach. Most contaminating molds were found in the circulating fans, tunnel and culture room floor. The bacterial isolates were collected from the air in mushroom culture room and killed with 0.005% Benzalkonium solution, indicating treatment of Benzalkonium are the effective methods to sanitize the mushroom culture room.

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Effect of Gamma Irradiation and Cichorium Products on Oxidative Damage and Lipid Metabolism in Streptozotocin-Induced Diabetic Rats (감마선 전신 조사와 치커리 가공물 식이가 Streptozotocin 유발 당뇨쥐의 산화적 손상과 지질대사에 미치는 영향)

  • Woo, Hyun-Jung;Kim, Ji-Hyang;Kim, Jin-Kyu;Kim, Hee-Jung;Park, Ki-Beom
    • Korean Journal of Environmental Biology
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    • v.24 no.2 s.62
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    • pp.102-111
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    • 2006
  • The increased occurrence of hyperglycemia and oxidative stress in streptozotocin (STZ) induced type I diabetes has been implicated in the etiology and pathology of disease complication. STZ has known to be genotoxic in a variety of assays including tests for microbial mutagenesis and unscheduled DNA synthesis in rat kidney. Diabetes mellitus (DM) is a pathologic condition, resulting in severe metabolic imbalances and non-physiologic changes in many tissues. We examined the effect of gamma radiation and KWNP on preventing the development of insulin dependent diabetes mellitus using streptozotocin-induced Fisher 344 diabetic rats. The hematological values (red blood cell and white blood cell), serum biochemical constituents-alkaline phosphatase (ALP), total cholesterol, triglycerides and insulin-were checked and the organs (testis, spleen and kidney) were weighed. The gonad indices of the STZ treated groups were much lower than the value of the control group. But the gonad indices of the KWNP treated groups were higher than those of the treated groups. The ratio of the weight of kidney to the body weight of the STZ treated groups was higher than that of the control group. The value of the diabetic group treated with KWNP after irradiation (F group) was lower than the other STZ treated groups. The white blood cell and ALP values of the F group were lower than the other STZ groups, as well. The cholesterol and triglyceride values of all the KWNP treated groups were significantly lower than the other groups. A significant increase (about 10 times) of insulin was detected in the F group. The results of hematological assay showed the distinctive damage in the irradiated and STZ treated groups. The quantity of apoptotic cells in seminiferous tubule of testis confirmed a serious damage as assessed in the STZ treated groups. These experimental results have revealed that treatment of the products of KWNP after irradiation has the antidiabetic effect in the STZ-induced diabetic rats. But the F group showed higher recuperative power. These experimental results have revealed that treatment of the gamma irradiation and KWNP have the recovering effect in the STZ-induced diabetic rats.

Degree of Self-Understanding Through "Self-Guided Interpretation" in Yeoncheon, Hantan River UNESCO Geopark: Focusing on Readability and Curriculum Relevance (한탄강 세계지질공원 연천 지역의 자기-안내식 해설 매체를 통한 스스로 이해 가능 정도: 이독성과 교육과정 관련성을 중심으로)

  • Min Ji Kim;Chan-Jong Kim;Eun-Jeong Yu
    • Journal of the Korean earth science society
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    • v.44 no.6
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    • pp.655-674
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    • 2023
  • This study examined whether the "self-guided interpretation" media in the Yeoncheon area of the Hantangang River UNESCO Geopark are intelligible for visitors. Accordingly, two on-site investigations were conducted in the Hantangang River Global Geopark in September and November 2022. The Yeoncheon area, known for its diverse geological features and the era of geological attraction formation, was selected for analysis. We analyzed the readability levels, graphic characteristics, and alignment with science curriculum of the interpretive media specific to geological sites among a total of 36 self-guided interpretive media in the Yeoncheon area. Results indicated that information boards, primarily offering guidance on geological attractions, were the most prevalent type of interpretive media in the Yeoncheon area. The quantity of text in explanatory media surpassed that of a 12th-grade science textbook. The average vocabulary grade was similar to that of 11th- and 12th-grade science textbooks, with somewhat reduced readability due to a high occurrence of complex sentences. Predominant graphic types included illustrative photographs, aiding comprehension of the geological formation process through multi-structure graphics. Regarding scientific terms used in the interpretive media, 86.3% of the terms were within the "Solid Earth" section of the 2015 revised curriculum, with the majority being at the 4th-grade level. The 11th-grade optional curriculum terms comprised the second largest portion, and 13.7% of all science terms were from outside the curriculum. Notably, variations in the scientific terminology's complexity was based on geological attractions. Specifically, the terminology level on the homepage tended to be generally higher than that on information boards. Through these findings, specific factors impeding visitor comprehension of geological attractions in the Yeoncheon area, based on the interpretation medium, were identified. We suggest further research to effect improvements in self-guided interpretation media, fostering geological resource education for general visitors and anticipating advancements in geology education.

A Study on the long-term Hemodialysis patient중s hypotension and preventation from Blood loss in coil during the Hemodialysis (장기혈액투석환자의 투석중 혈압하강과 Coil내 혈액손실 방지를 위한 기초조사)

  • 박순옥
    • Journal of Korean Academy of Nursing
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    • v.11 no.2
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    • pp.83-104
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    • 1981
  • Hemodialysis is essential treatment for the chronic renal failure patient's long-term cure and for the patient management before and after kidney transplantation. It sustains the endstage renal failure patient's life which didn't get well despite strict regimen and furthermore it becomes an essential treatment to maintain civil life. Bursing implementation in hemodialysis may affect the significant effect on patient's life. The purpose of this study was to obtain the basic data to solve the hypotension problem encountable to patient and the blood loss problem affecting hemodialysis patient'a anemic states by incomplete rinsing of blood in coil through all process of hemodialysis. The subjects for this study were 44 patients treated hemodialysis 691 times in the hemodialysis unit, The .data was collected at Gang Nam 51. Mary's Hospital from January 1, 1981 to April 30, 1981 by using the direct observation method and the clinical laboratory test for laboratory data and body weight and was analysed by the use of analysis of Chi-square, t-test and anlysis of varience. The results obtained an follows; A. On clinical laboratory data and other data by dialysis Procedure. The average initial body weight was 2.37 ± 0.97kg, and average body weight after every dialysis was 2.33 ± 0.9kg. The subject's average hemoglobin was 7.05±1.93gm/dl and average hematocrit was 20.84± 3.82%. Average initial blood pressure was 174.03±23,75mmHg and after dialysis was 158.45±25.08mmHg. The subject's average blood ion due to blood sample for laboratory data was 32.78±13.49cc/ month. The subject's average blood replacement for blood complementation was 1.31 ±0.88 pint/ month for every patient. B. On the hypotensive state and the coping approaches occurrence rate of hypotension was 28.08%. It was 194 cases among 691 times. 1. In degrees of initial blood pressure, the most 36.6% was in the group of 150-179mmHg, and in degrees of hypotension during dialysis, the most 28.9% in the group of 40-50mmHg, especially if the initial blood pressure was under 180mmHg, 59.8% clinical symptoms appeared in the group of“above 20mmHg of hypotension”. If initial blood pressure was above 180mmHg, 34.2% of clinical symptoms were appeared in the group of“above 40mmHg of hypotension”. These tendencies showed the higher initial blood pressure and the stronger degree of hypotension, these results showed statistically singificant differences. (P=0.0000) 2. Of the occuring times of hypotension,“after 3 hrs”were 29.4%, the longer the dialyzing procedure, the stronger degree of hypotension ann these showed statistically significant differences. (P=0.0142). 3. Of the dispersion of symptoms observed, sweat and flush were 43.3%, and Yawning, and dizziness 37.6%. These were the important symptoms implying hypotension during hemodialysis accordingly. Strages of procedures in coping with hypotension were as follows ; 45.9% were recovered by reducing the blood flow rate from 200cc/min to 1 00cc/min, and by reducing venous pressure to 0-30mmHg. 33.51% were recovered by controling (adjusting) blood flow rate and by infusion of 300cc of 0,9% Normal saline. 4.1% were recovered by infusion of over 300cc of 0.9% normal saline. 3.6% by substituting Nor-epinephiine, 5.7% by substituting blood transfusion, and 7,2% by substituting Albumin were recovered. And the stronger the degree of symptoms observed in hypotention, the more the treatments required for recovery and these showed statistically significant differences (P=0.0000). C. On the effects of the changes of blood pressure and osmolality by albumin and hemofiltration. 1. Changes of blood pressure in the group which didn't required treatment in hypotension and the group required treatment, were averaged 21.5mmHg and 44.82mmHg. So the difference in the latter was bigger than the former and these showed statistically significant difference (P=0.002). On the changes of osmolality, average mean were 12.65mOsm, and 17.57mOsm. So the difference was bigger in the latter than in the former but these not showed statistically significance (P=0.323). 2. Changes of blood pressure in the group infused albumin and in the group didn't required treatment in hypotension, were averaged 30mmHg and 21.5mmHg. So there was no significant differences and it showed no statistical significance (P=0.503). Changes of osmolality were averaged 5.63mOsm and 12.65mOsm. So the difference was smaller in the former but these was no stitistical significance (P=0.287). Changes of blood pressure in the group infused Albumin and in the group required treatment in hypotension were averaged 30mmHg and 44.82mmHg. So the difference was smaller in the former but there is no significant difference (P=0.061). Changes of osmolality were averaged 8.63mOsm, and 17.59mOsm. So the difference were smaller in the former but these not showed statistically significance (P=0.093). 3. Changes of blood pressure in the group iutplemented hemofiltration and in the Uoup didn't required treatment in hypotension were averaged 22mmHg and 21.5mmHg. So there was no significant differences and also these showed no statistical significance (P=0.320). Changes of osmolality were averaged 0.4mOsm and 12.65mOsm. So the difference was smaller in the former but these not showed statistical significance(P=0.199). Changes of blood pressure in the group implemented hemofiltration and in the group required treatment in hypotension were averaged 22mmHg and 44.82mmHg. So the difference was smatter in the former and these showed statistically significant differences (P=0.035). Changes of osmolality were averaged 0.4mOsm and 17.59mOsm. So the difference was smaller in the former but these not showed statistical significance (P=0.086). D. On the changes of body weight, and blood pressure, between the group of hemofiltration and hemodialysis. 1, Changes of body weight in the group implemented hemofiltration and hemodialysis were averaged 3.340 and 3.320. So there was no significant differences and these showed no statistically significant difference, (P=0.185) but standard deviation of body weight averaged in comparison with standard difference of body weight was statistically significant difference (P=0.0000). Change of blood Pressure in the group implemented hemofiltration and hemodialysis were averaged 17.81mmHg and 19.47mmHg. So there was no significant differences and these showed no statistically significant difference (P=0.119), But in comparison with standard deviation about difference of blood pressure was statistically significant difference. (P=0.0000). E. On the blood infusion method in coil after hemodialysis and residual blood losing method in coil. 1, On comparing and analysing Hct of residual blood in coil by factors influencing blood infusion method. Infusion method of saline 200cc reduced residual blood in coil after the quantitative comparison of Saline Occ, 50cc, 100cc, 200cc and the differences showed statistical significance (p < 0.001). Shaking Coil method reduced residual blood in Coil in comparison of Shaking Coil method and Non-Shaking Coil method this showed statistically significant difference (P < 0.05). Adjusting pressure in Coil at OmmHg method reduced residual blood in Coil in comparison of adjusting pressure in Coil at OmmHg and 200mmHg, and this showed statistically significant difference (P < 0.001). 2. Comparing blood infusion method divided into 10 methods in Coil with every factor respectively, there was seldom difference in group of choosing Saline 100cc infusion between Coil at OmmHg. The measured quantity of blood loss was averaged 13.49cc. Shaking Coil method in case of choosing saline 50cc infusion while adjusting pressure in coil at OmmHg was the most effective to reduce residual blood. The measured quantity of blood loss was averaged 15.18cc.

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