• 미생물학회지
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• 제23권4호
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• pp.265-270
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• 1985

Brevibacterium flavum과 Corllnebac ter ium glutamicum세포로부터의 원형질쳐l 형성과정, 형성된 원형질체의 미세구조, 이들 원형질체간의 융합 및 세포벽 재생을 투과전자현미경을 사용하여 관찰하였다. 원형질쳐1의 형성과 정은 특이하게 관찰되었다. 즉, penicillin G와 lysozyme의 작용을 받아 세포벽의 한 부위가 파괴되어 생긴 細孔응 통하여 원형질이 빠져나가 구형의 원형질체를 형성하였닥 융합과정에서는 원형질체들이 서로 접촉하여 접촉부꾀 를 형성하고 이어서 이 부위의 셰포막이 얼부 소실되며 양 원형질쳐1간에 내부의 울질들이 이동하였다 융합 원 행질체들은 비융합 원형질체에 비하여 세포벽의 재생이 다소 더디게 일어나는 것으로 관찰되었으며, 융합 원형질 쳐l들이 비융합 원형질체들 보다 그 크기가 월등히 컸다.

• Journal of Yeungnam Medical Science
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• 제38권2호
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• pp.152-156
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• 2021

Diplopia is a rare complication of spine surgery. The abducens nerve is one of the cranial nerves most commonly related to diplopia caused by traction injury. We report a case of a 71-year-old woman who presented with diplopia developing from abducens nerve palsy after C1-C2 fixation and fusion due to atlantoaxial subluxation with cord compression. As soon as we discovered the symptoms, we suspected excessive traction by the instrument and subsequently performed reoperation. Subsequently, the patient's symptoms improved. In other reported cases we reviewed, most were transient. However, we thought that our rapid response also helped the patient's fast recovery in this case. The mechanisms by which postoperative diplopia develops vary and, thus, remain unclear. We should pay attention to the fact that the condition is sometimes an indicator of an underlying, life-threatening condition. Therefore, all patients with postoperative diplopia should undergo thorough ophthalmological and neurological evaluations as well as careful observation by a multidisciplinary team.

• 공학교육연구
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• 제25권2호
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• pp.32-41
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• 2022

The purpose of this study was in a pandemic situation caused by COVID-19 to explore the online distance experiments and interaction of engineering freshmen, and to identify practical difficulties, resulting in to derive implications. Seven freshmen from engineering college participated in the interview, of which data were analyzed based on phenomenological analysis methods. The types of non-face-to-face experiments experienced by students were complete non-face-to-face experiment, mixed face-to-face experiment, and fusion face-to-face experiment. Students were completely isolated in time and space in complete non-face-to-face experiment. In biweekly mixed face-to-face experiment, isolation was halved. In fusion face-to-face experiment, isolation was removed. Non-face-to-face interactions can be characterized by restrictions on simultaneous activities, on rapport formation, and on observation opportunities. Based on these results, three implications were derived: First, it is necessary to allow students to manage time and space constraints on their own in non-face-to-face experiments. Second, support is needed to solve the difficulty of forming rapport, which is a characteristic of non-face-to-face interaction. Third, an opportunity to observe the interaction between other students and professors should be provided.

• Nuclear Engineering and Technology
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• 제52권4호
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• pp.827-834
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• 2020

Damage of tungsten due to helium ions of a PF device was studied. The tungsten was analyzed by SEM and AFM after irradiation. SEM revealed fine bubbles of helium atoms with diameters of a few nanometers, which join and form larger bubbles and blisters on the surface of tungsten. This observation confirmed the results of molecular dynamics simulation. SEM analysis after etching of the irradiated surface indicated cavities with depth range of 35-85 nm. The average fluence of helium ion of the PF device was calculated about 5.2 × 10¹⁵ cm^-2 per shot, using Lee code. Energy spectrum of helium ions was estimated using a Thomson parabola spectrometer as a function of dN/dE ∝ E^-2.8 in the energy range of 10-200 keV. The characteristics of helium ion beam was imported to SRIM code. SRIM revealed that the maximum DPA and maximum helium concentration occur in the depth range of 20-50 nm. SRIM also showed that at depth of 30 nm, all of the tungsten atoms are displaced after 20 shots, while at depth of higher than 85 nm the destruction is insignificant. There is a close match between SRIM results and the measured depths of cavities in SEM images of tungsten after etching.

• 한국측량학회지
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• 제36권4호
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• pp.263-270
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• 2018

지표면 온도는 지면-대기의 상호 순환을 이해하는데 중요한 요소로 알려져 있지만 시공간적 변동성이 크기 때문에 정규적인 관측은 거의 이루어지지 못하고 있다. 기존의 지표면 온도는 위성 영상을 이용하여 관측하고 있지만 위성의 특성상 긴 재방문주기와 낮은 정확도의 한계를 가지고 있다. 본 연구에서는 기존의 위성 영상을 활용한 지표면 온도 관측의 대체가능성을 확인하기 위해 무인항공기에 열적외선 센서를 탑재하여 단일 영상을 취득하였다. 취득된 영상은 JPEG 영상에서 TiFF 영상으로 변환하여 정사영상을 제작하였으며 정사영상의 DN값을 이용하여 실제 지표면 온도로 계산하였다. 계산된 피복별 지표면 온도의 정확도를 평가하기 위해 영상촬영과 동시에 적외선 온도계로 직접 관측한 지표면 온도와 비교하였다. 두 가지 방법으로 관측한 지표면 온도를 비교 했을 때, 모든 피복들에 대해서 정확도가 열적외선 센서의 관측 정확도 이하로 나타났다. 따라서 무인항공기에 탑재된 열적외선 센서를 이용하여 기존의 지표면 온도 관측 방법인 위성 영상의 대체 가능성을 확인하였다.

• 대한수의학회지
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• 제29권2호
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• pp.99-108
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• 1989

In order to observe the pathological changes of kidney in rabbits infected with the viral haemorrhagic disease, the kidney tissues from the 91 rabbits infected with the viral haemorrhagic disease were examined by light and electron microscopy. The results observed were as follows: 1. On light microscopic observation, the kidney lesions were identified as haemorrhagic glomerular necrosis(33.0%), membranous glomerulonephritis(20.9%), thrombotic glomerulopathy(19.8%), membranoproliferative glomerulonephritis(8.8%), mesangial proliferative glomerulonephritis(8.8%) ischemic acute tubular necrosis(7.7%), and acute serous glomerulitis(6.6%). 2. On electron microscopic observation, cytoplasmic degeneration of mesangial cells, and irregular thickening of basement membranes with electron dense granular materials were observed. In podocytes swelling of mitochondria, dilatation of endoplasmic reticulum and extensive fusion of foot processes were also observed. Nonenveloped round icosahedral picornaviral particles with a diameter of 28~33nm were detected in the cytoplasm of degenerative endothelial cells, polymorphonuclear leucoytes, and monocytes.

• Journal of Microbiology and Biotechnology
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• 제6권6호
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• pp.468-473
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• 1996

The genome of tomato spotted wilt virus (TSWV) is composed of three RNA segments, S, M, and L RNA and the 5.0 kb M RNA encodes two glycoproteins Gl, G2 and NSm protein of unknown function. In an effort to investigate the function of the NSm protein, antibody was raised against NSm fusion protein overexpressed in Escherichia coli. This antibody was used to detect the NSm protein by using western blot analysis and electron microscopic observation after immunogold labelling. For the cloning of the NSm gene, total RNA extracted from a TSWV infected plant was used for cDNA synthesis and polymerase chain reaction (PCR) instead of going through time-consuming virus purification. A protein band specifically reacting to the NSm antibody was detected from TSWV inoculated plants. The NSm protein was detected in the cell wall fraction and in pellet from low speed centrifugation when the infected plant tissue was fractionated into 4 fractions. In the immuno-electron microscopic observation, gold particles were found around the plasmodesmata of infected plant tissue. These results suggest that the NSm protein of TSWV plays some role in cell-to-cell movement of this virus.

• 폴리머
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• 제32권2호
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• pp.109-115
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• 2008

탈미네랄화된 골분(DBP)은 사이토카인과 같은 다양한 생리활성분자를 가지기 때문에 조직공학분야에서 널리 사용되는 생체재료이다. 본 연구에서는 DBP를 함유한 2차원적 DBP/PLGA 필름이 추간판디스크 세포의 부착, 증식 및 표현형유지에 미치는 영향에 대해 연구하였다. DBP 함량에 따른 DBP/PLGA 필름은 용매증발법으로 제조하였으며 제조된 PLGA 및 DBF/PLGA 필름은 시차주사현미경을 통해 표면을 분석하였다. PLCA 필름은 매끄러운 표면을 가지며, DBF의 함량이 증가할수록 DBP/PLGA 필름의 표면은 거침도가 증가하는 것을 확인하였다. 섬유륜(AF) 및 수핵(NP)세포를 PLGA 및 DBP/PLCA 필름 표면에 파종하여 배양한 후, 세포의 계수 및 SEM 관찰을 통하여 이들의 부착과 증식을 평가하였다. 세포 계수와 SEM 관찰 결과, DBP의 함량이 10 및 20%인 DBP/PLGA 필름에서 높은 초기부착도 및 증식률을 보였다. 세포 계수 결과를 바탕으로 RT-PCR을 통하여 DBF 10%와 20%의 DBP/PLGA 필름에서의 디스크 세포의 특이적 유전자 발현확인 결과, DBF의 함량이 20%인 DBP/PLCA 필름에서 세포의 표현형이 유지되며 지속적인 세포외기질이 발현될 것으로 예상되었다. 따라서 적절한 천연재료의 함량이 세포의 부착과 증식에 더욱 적합하며 이는 조직공학적 디스크 재생의 기초 자료로 사용될 것으로 사료된다.

• Journal of the Korean Data and Information Science Society
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• 제18권4호
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• pp.879-890
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• 2007

Statistical matching is a method of data integration for data sources that do not share the same units. It could produce rapidly lots of new information at low cost and decrease the response burden affecting the quality of data. This paper proposes a statistical matching technique combining k-NN (k-nearest neighborhood) and regression methods. We select k records in a donor file that have similarity in value with a specific observation of the common variable in a recipient file and estimate an imputation value for the recipient file, using regression modeling in the donor file. An empirical comparison study is conducted to show the properties of the proposed method.

• 천문학회지
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• 제44권3호
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• pp.73-79
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• 2011

We collect 24 times of light maxima data from sources in the literature, unpublished data and open databases, and investigate the variations of the observed and calculated (O-C) values for light maxima of V1719 Cyg. We found no evidence of the variations in the (O-C) values. We estimate the effective temperature and surface gravity using both the Kurucz and MARCS/SSG grids for different metallicity values [A/H]=0.0 and +0.5 for V1719 Cyg. It is confirmed that the temperature is almost the same, but, in the case of surface gravity, the MARCS/SSG grid gives the value closest to that obtained from the period-gravity relation derived by using the pulsation-evolution theory. We obtain two spectra of V1719 Cyg from spectroscopic observation which permitted us to find the effective temperature and the surface gravity of the star directly. We estimate the metallicity and it is found that the abundance of iron is equal to the solar value.