• 한국임상약학회지
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• 제15권2호
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• pp.118-126
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• 2005

In our study, osteoporosis was induced by ovariectomized in female Sprague-Daweley (SD) rats, and the prevention and treatment efficacy of the climacteric disease the ostmenopausal type I pattern was examined by using the experimental substance soybeans and arowroot. No clinical signs and mortality after continuous oral administration of experimental substances for 14 weeks were not observed, however, the weight of experimental animals underwent ovariectomized was increased significantly in comparison with control group (p<0.01). The atrophy of the uterus due to T3 group was 23.64% after 7 weeks, 24.44% after 14 weeks (in comparison with N.C. group), and it was statistically significantly increased. In regard to the change of blood cells, it was observed that platelets were statistically significantly reduced in the ovariectomized group, and administered orally experimental substances continuously for 7 weeks, in all groups administered experimental substances, it was found that platelets had the tendency to be increased more than N. C. group. In regard to He change of blood biochemistry, removed the ovary, the concentration of ALP showed the tendency to be increased than control group, and particularly in T3 group, it was increased significantly. In regard to the concentration of cholesterol, in comparison with negative control group, it was reduced 68% at 7 weeks and 35% at 14 weeks. After ovariectomized, the amount of estrogen was found to be reduced by 21.37% in comparison with control group, it showed the tendency to be increased by 4.49% in T1 group and 7.62% in T2 group, the concentration of estrogen in each group showed the tendency to be increased than negative control group, and in T3 group, it was increased to 100.46% and 117.65% in T4 group, and it was increased more than control group. Based on the above experimental results, in the experimental animals female rats, because of the hormonal imbalance induced by ovariectomized, a large mount of fat is accumulated in the body and due to it osteoporosis, obesity, hypertension, hyperlipidemia, fat of the liver, arteriosclerosis, diabetes, and other metabolic diseases were developed. Hence, when the experimental substance Extraction of ferment arrowroot was orally administered continuously for 14 weeks, it was thought that a certain proportion of the hormonal balance was maintained that functioned as a substance interfering the accumulation of fat, and it was considered to be of help in the treatment of not only osteoporosis Type I, but also for the prevention and treatment of various endocrinal diseases.

• 동의생리병리학회지
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• 제19권5호
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• pp.1296-1302
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• 2005

Laminariae Thallus has been known to be effective for the treatment of obesity. Te show the effectiveness of Laminariae Thallus in a more scientific way, Laminariae Thallus extract was prepared and evaluated in high fat diet rats by measuring the changes of body weight and lipid metabolism as described briefly below. 200 g of crushed Laminariae Thallus was extracted with methyl alcohol. The extract was evaporated under reduced pressure to give 14.4g. For 10 weeks, control group rat were ingested high fat diet, while the test group rat were fed with a fat diet pius Laminariae Thallus extract. Normal group was fed with normal diet. 150 mg of Laminariae Thallus extract per 1 kg of body weight was added to the diet in a test group rats. Control group rats on a high fat diet gained weight significantly, whereas the test group rats on a high fat diet plus Laminariae Thallus extract gained weight less. Significant increase of liver weight caused in a high fat diet was also inhibited by the Laminariae Thallus extract treatment. Total lipid, triglyceride, phospholipid and total cholesterol levels of serum in the high fat diet rats were remarkably increased, whereas their levels on a high fat diet plus Laminariae Thallus extract were less increased. While serum HDL-cholesterol levels was remarkably decreased in a high fat diet, its level was less decreased in a high fat diet plus Laminariae Thallus extract. Furthermore, we observed that the activities of hepatic acetyl-CoA carboxylase and fatty acid synthetase increased in a high fat diet, while their activities in a high fat diet plus Laminariae Thallus extract were getting back nearly to the normal levels of normal diet rats. These result showed that the obesity caused by a high fat diet was effectively inhibited by a Laminariae Thallus extract. Our results also showed that the abnormal lipid metabolism caused by a high fat diet was effectively cured by adding Laminariae Thallus extract.

• Nutrition Research and Practice
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• 제12권4호
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• pp.298-306
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• 2018

BACKGROUND/OBJECTIVES: Obesity is a global health problem of significant importance which increases mortality. In place of anti-obesity drugs, natural products are being developed as alternative therapeutic materials. In this study, we investigated the effect of Brassica juncea L. leaf extract (BLE) on fat deposition and lipid profiles in high-fat, high-cholesterol diet (HFC)-induced obese rats. MATERIALS/METHODS: Male Sprague-Dawley rats were divided into four groups (n = 8 per group) according to diet: normal diet group (ND), high-fat/high-cholesterol diet group (HFC), HFC with 3% BLE diet group (HFC-A1), and HFC with 5% BLE diet group (HFC-A2). Each group was fed for 6 weeks. Rat body and adipose tissue weights, serum biochemical parameters, and tissue lipid contents were determined. The expression levels of mRNA and proteins involved in lipid and cholesterol metabolism were determined by reverse transcription polymerase chain reaction and western blot analysis, respectively. RESULTS: The HFC-A2 group showed significantly lower body weight gain and food efficiency ratio than the HFC group. BLE supplementation caused mesenteric, epididymal, and total adipose tissue weights to decrease. The serum levels of triglyceride, total cholesterol, and low-density lipoprotein cholesterol were significantly reduced, and high-density lipoprotein cholesterol was significantly increased in rats fed BLE. These results were related to lower glucose-6-phosphate dehydrogenase, acetyl-coA carboxylase, and fatty acid synthase mRNA expression, and to higher expression of the cholesterol $7{\alpha}$-hydroxylase and low density lipoprotein-receptor, as well as increased protein levels of peroxisome proliferator-activated receptor ${\alpha}$. Histological analysis of the liver revealed decreased lipid droplets in HFC rats treated with BLE. CONCLUSIONS: Supplementation of HFC with 3% or 5% BLE inhibited body fat accumulation, improved lipid profiles, and modulated lipogenesis- and cholesterol metabolism-related gene and protein expression.

• 한국식품영양학회지
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• 제32권6호
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• pp.711-716
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• 2019

Red Chinese cabbage is rich in anthocyanins, with beneficial effects on anti-obesity and lipid profiles. The purpose of this study was to examine the anti-obesity effects of red Chinese cabbage. Four-weeks old Spague-Dawley male rats were divided into four groups for six weeks as follow: Normal Diet (ND), High Fat Diet (HFD), HFD + Red Chinese Cabbage Diet (RCD), HFD + Red Chinese Cabbage Juice (RCJD). After six weeks, the liver weight was significantly higher in the HFD group than in the other groups, and the RCD and RCJD groups showed similar values to the ND group. The epididymal fats were significantly higher in all high fat diets (HFD, RCD, RCJD) than in the ND group and there was statistical significance in the post-tests when comparing the RCD and RCJD groups with the HFD group. Triglycerides significantly decreased in the RCD group. In conclusion, red Chinese cabbage is rich in anthocyanins, with beneficial effects on organ weight and lipid profiles.

• 한국자원식물학회지
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• 제29권5호
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• pp.620-624
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• 2016

본 연구에서 국내 자생식물 추출물중 비만관련 특허가 없는 식물원료를 대상으로 지방세포의 분화억제 효과를 Wnt/β-catenin 신호전달계 활성측정방법으로 탐색하여 비만치료를 위한 기능성소재 응용가능성을 검토하였다. HEK 293-TOP세포의 luciferase 리포터 상대적인 활성은 무 처리 대조군에 비하여 지유(전초), 측백나무(줄기)가 각각 152%, 130%로 높은 활성을 나타내었으며, 피마자(전초)는 약 90%대의 활성을, 해당화(줄기), 괴화(전초)의 경우는 약 80%, 초피나무(줄기), 칡(줄기), 까마중(전초)은 약 70%대의 높은 활성을 나타내었다. 또한, 신경줄기 세포에 대한 어떠한 독성도 보이지 않음으로써 안전한 물질인 것으로 사료되었다. 이 결과는 Wnt/β-catenin 신호전달 활성 측정방법이 향후 High throughput screening 기반기술로 활용될 수 있을 것으로 판단되며, 지방세포 분화 억제활성 후보로 선별된 식물추출물들에 대한 추가적인 실험을 통하여 항비만 소재 개발 응용 가능성을 타진할 것이다.

• Journal of Nutrition and Health
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• 제57권2호
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• pp.185-195
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• 2024

Purpose: The carbohydrate-restricted diet has been recognized to be effective into preventing and alleviating lifestyle-related diseases, such as obesity and type 2 diabetes. The rare sugar ᴅ-allulose is a functional monosaccharide with anti-obesity effects. In the present study, we examined the effects of dietary ᴅ-allulose on body fat accumulation in rats fed severely carbohydrate-restricted diets containing high concentrations of different fats, beef tallow, or soybean oil. Methods: Male Wistar rats (n = 35, 3-week-old) were divided into 5 groups: One chow-fed control (C) group, and four carbohydrate-restricted groups, namely, beef tallow (B), beef tallow + ᴅ-allulose (BA), soybean oil (S), and soybean oil + ᴅ-allulose (SA), with free access to the diet and water for 8 weeks. The B and BA diets contained 23% beef tallow and 2% soybean oil, whereas the S and SA diets contained 25% soybean oil. Furthermore, the BA and SA diets contained 5% ᴅ-allulose. Results: The final body weight, weight gain, and food intake were significantly higher, and food efficiency was significantly lower in the control group compared to the other carbohydrate-restricted groups. Intra-abdominal adipose tissue, carcass fat, and total body fat weights were not influenced by dietary fat type or ᴅ-allulose supplementation, except for the epididymal adipose tissue weight. In contrast, carbohydrate restriction suppressed body weight gain in rats, but remarkably increased body fat accumulation. Conclusion: Under carbohydrate-restricted conditions, no anti-obesity effects of dietary ᴅ-allulose were observed, regardless of the dietary fat type. The causes of these effects are unknown. However, they may be influenced by a very low carbohydrate and high protein diet. Further research is required to elucidate the effects of ᴅ-allulose under various nutrient compositions with different fat, carbohydrate, and protein energy ratios.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.242-247
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• 2005

Caseinomacropeptide (CMP) is a glycopeptide of 64 amino acid residues derived from the C-terminal of mammalian milk K-casein. Recently, human CMP (hCMP) was produced from the recombinant yeast Saccharomyces cerevisiae. In this study, the antiobesity activity of the recombinant hCMP (rhCMP) was investigated in vivo using Sprague-Dawley rats. The rhCMP did not affect the rats fed with a normal fat diet (fat content, $5.0\%$), but decreased the body weight gain of the rats fed with a high fat diet (fat content, $20\%$) by up to $19\%$. Autopsies revealed that the weights of the liver, kidney and adipose tissues decreased when the rats were given the rhCMP, which also reduced the lipid concentrations in the plasma and liver, but enhanced the fecal excretion of lipids. These results suggest that rhCMP prevent the accumulation of lipid by stimulating its fecal excretion, so could be used as a food supplement to alleviate the obesity problem caused by a high fat diet.

• Toxicological Research
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• 제21권3호
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• pp.255-261
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• 2005

Gami-Samhwang-San, a herbal prescription for obesity treatment, is composed of seven crude herbs such as Rehmanniae Radix Preparata, Ephedrae Herba, Scutellariae Radix, Acori Gramineri Rhizoma, Polygalae Radix, Typhae Pollen, Armeniacae Semen, Menthae Herba. In this study, marker substances in n-butanol fraction (SH-21-B) from Gami-Samhwang-San were analyzed by high performance liquid chromatography (HPLC) and acute toxicity of standardized SH-21-B was evaluated by good laboratory practices (GLP) guideline of Korea Food and Drug Administration. Therefore we confirmed that there were baicalin of 15.92%, amygdalin of 6.57% and ephedrine of 2.49% in SH-21-B. SH-21-B was administered in rats at dose of 0 mg/kg, 2,000 mg/kg, and 5,000mg/kg. Clinical signs of both sexes of rats were observed daily for 14 days after single oral administration. Two female rats one administered at 2,000 mg/kg and the other administered at 5,000 mg/kg, died, but no dead animal was observed among male rats. Therefore $LD_{50}$ in the female rat is observed to be 8,710 mg/kg, and MLD (Minimun Lethal Dose) of the male rat is observed to be more than 5,000 mg/kg.

• 생명과학회지
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• 제19권9호
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• pp.1265-1270
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• 2009

능이버섯의 항비만 건강식품으로서의 이용가능성을 분석하였다. 고지방식이에 의해 유도된 비만쥐에 6주 동안 능이버섯 분말을 섭취시켜, 능이버섯의 효과를 알아보기 위하여 비만지수, 혈청지질, 장기무게 등의 여러 가지 요인들을 분석하였다. 고지방식이를 먹인 쥐 혈청의 중성지방, 총콜레스테롤 및 Ldl은 유의적으로 증가하였으나 능이버섯분말을 먹인 쥐는 증가하지 않았다. 고지방식이 쥐에서 R$\ddot{o}$hrer index는 대조군에 비해 유의적으로 증가하였으나 버섯 분말을 섭취한 쥐는 R$\ddot{o}$hrer index가 유의적으로 감소하였다. 특히 몸무게에 대한 간 무게의 비율은 대조군에 비해 비만식이 쥐에서 높았다. 이러한 결과들은 버섯을 먹인 쥐에서의 비만지수와 장기무게의 유의적인 변화는 혈청에서 중성지방의 감소 때문인 것으로 생각된다. 그렇기 때문에 능이버섯은 비만과 관련된 질병을 막을 수 있는 건강식품의 하나로 이용 될 수 있는 가능성이 있다.

• 동의생리병리학회지
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• 제23권3호
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• pp.562-566
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• 2009

The aim of this study was to investigate the effects of herbal remedy using onion (HRO-1) supplementation on body weight change and cholesterol, triglyceride. free fatty acid, total lipid, phospholipid serum level in obesity mice. This study classified 32 of 4 weeks-old male C57BL/6 mice which have obesity caused by high fat fed into four groups of 8 mice and made the subjects medicated them HRO-1 for 6 weeks. Male C57BL/6 mice were divided into four groups; a normal group, a high-fat group, a high-fat group supplemented with HRO-1 (500 mg/kg), a high-fat group supplemented with HRO-1 (1000 mg/kg). In respect to the body weight change, there was statistically significant difference between the HRO-1 (1000 mg/kg) group compared to high-fat group (p<0.05). In the total cholesterol and LDL cholesterol level, there was statistically significant difference between the HRO-1 (1000 mg/kg) group compared to high-tat group (p<0.05). In the triglyceride and free fatty acid level, there was statistically significant difference between the normal group compared to high-fat group (p<0.05). In the total lipid and phospholipid level, there was statistically significant difference between the normal group compared to high-fat group (p<0.05). Also in the total lipid level, there was statistically significant difference between the HRO-1 group compared to high-fat group (p<0.05). The above results suggests that HRO-1 medicated is effective to prevention and treatment of obesity.