• The Korean Journal of Mycology
• /
• v.44 no.4
• /
• pp.300-306
• /
• 2016

Recently, the incidence of oak wilt disease has been increasing in Korea, resulting in an increasing number of dead trees. In this study, we performed sawdust bag cultivation of shiitake (Lentinula edodes) using oak wilt-diseased logs and measured the antioxidant activities of the resulting mushrooms. For sawdust media, 4 types of logs (healthy, infected, dead, and fumigated ones) were used. As inocula, three strains of L. edodes (NIFoS 764, Sanjo 701ho, and Sanmaru 2ho) were used. The productivity of L. edodes from dead logs was similar to that from healthy logs. Except for the Sanmaru 2ho strain, fumigated logs and infected logs yielded a lower productivity of L. edodes compared to healthy logs. In terms of antioxidant activity, L. edodes grown on sawdust from fumigated logs showed higher values than those from other logs.

• Korean Journal of Remote Sensing
• /
• v.33 no.5_3
• /
• pp.879-888
• /
• 2017

This study is a preliminary research conducted in Buhansan mountain National Park to develop a management system to predict and control oak wilt disease by indicating spatial factors which affect diffusion of the disease. After analysing altitude factor during the estimation of spatial analysis of damaged area, it is indicated that damaged trees are mainly distributed at altitude of 200-500 m and number decreased drastically over the altitude of 500 m. The result showed that 92% of total damaged trees are on slope between 20~40 degrees and the number decreased drastically on slope steeper than 40 degrees. It is indicated that damaged area is mainly distributed on southern aspect. It is estimated by using CART that slope factor affected the diffusion of disease mostly but aspect factor did not. Surface temperature and altitude showed similar effect.By simulating possible diffusion scenario, it is estimated that the disease could spread to DO-BONG Mt., northeast of Bukhansan mountain.

• Research in Plant Disease
• /
• v.16 no.3
• /
• pp.219-223
• /
• 2010

Recently, the oak wilt diseases especially on Quercus mongolica, have been increasing in various districts of Korea. A collection of 38 strains of the oak wilt pathogen Raffaelea qeurcus-mongolicae and R. quecivora isolated from Quercus spp. in Korea and Japan was characterized by $\beta$-tubulin gene sequence and randomly amplified polymorphic DNA (RAPD) analysis. In cluster analysis based on $\beta$-tubulin gene sequence the strains were divided into 4 clusters, of which clusters 2 and 4 were composed of Japanese strains except for one Korean strain. RAPD analysis showed that they were also effectively differentiated by a strong RAPD fragments. On the basis of the two genetic analysis, significant differences were detected between Korean strains and Japanese strains.

• Korean journal of applied entomology
• /
• v.49 no.2
• /
• pp.145-149
• /
• 2010

Differences in the number of Platypus koryoensis (Murayama) (Coleoptera: Platypodidae) trapped in different trap types, colors and positions on the trunk were evaluated to develop a monitoring trap for Platypus koryoensis, a known vector of Korean oak wilt disease. The experiments were conducted in an oak forest located in Gwangtan-myeon, Paju-si, Gyeonggi-do, Korea using two types of trap: sticky trap and multi-funnel trap. Trapping efficiency of the two trap types was not significantly different. Trapping efficiency of the sticky trap was not affected by four trap colors: yellow, black, white and transparent. The number of beetle caught was higher in the traps facing the upper slope than the lower slope regardress of trap type. Our results showed that both traps type are useful for monitoring Platypus koryoensis.

• Korean Journal of Environment and Ecology
• /
• v.27 no.6
• /
• pp.704-717
• /
• 2013

This study aims to provide basic data for management and prevention of infection damage by Oak wilt disease through mapping method of status with infected level in damaged area of Bukhansan National Park. Survey was carried out in the distributed area of oak trees with mapping unit of polygon of actual vegetation and mapped of infection ratio and infection index applying weight according to infected level. Infection ratio of oak trees in Bukhansan National Park was 58.5%, and lightly damaged ratio was 29.6%, half of the damaged ratio was 16.1%, seriously damaged ratio was 8.8% and withered ratio was 4.1%. It was serious to be infected from Beomgol ridge in Wondobong district to Hyeongjaebong in Jeongrung district. Although the infected ratio of the western part of Songchu district, Sanseong district and Gugi district centering main ridge of Bukhansan National Park was low, it of ridge and main valley was high. Infection index of hardly damaged area was 39.1% of whole area, and slightly damaged area was 41.0%, half of the damaged area was 16.1%, seriously damaged area was 3.3% and alarmed withering area was 0.4%. Infection index was high around Musugol valley in Dobong district and Jaunbong, and it of Bohyunbong of Jeongrung district and the part of Hyojari valley of Sanseong district was serious. Predicted numbers of the trees affected Oak wilt disease compared to the distributed area of oak trees was 1,585,937 trees among 2,709,147 trees of Quercus spp. 352,931 trees among the 306,161 trees of oak were infected in Woi district, the most seriously infected area and 53,141 trees among the 145,747 trees of oak was infected in Gugi district, the most slightly infected area.

• 한국균학회소식:학술대회논문집
• /
• 2014.05a
• /
• pp.41-41
• /
• 2014

Pinewood nematode (PWN, Bursaphelenchus xylophilus) is a serious pathogenic worm that quickly dry pine trees to death. Recently, PWN has been devastating huge amounts of conifer trees in Korea. As a first step to explore the association and ecological roles of fungi in PWN life cycle in Korea, in this study we first isolated and indentified fungi from PWN-infested Korean pine and Japanese black pine wood sampled in Jinju, Sacheon, Pocheon, Chuncheon, Gwangju, and Hoengseong in Korea. A total of 144 fungal isolates were obtained from Japanese black pine wood and 264 fungal isolates from Korean pine wood. Their morphology and nucleotide sequences of the ITS rDNA and ♌-tubulin gene were examined for species identification. Ophiostoma ips, Botrytis anthophila, Penicillium sp., Hypocrea lixii, Trichoderma atroviride, O. galeiforme, Fusarium proliferatum were identified from Japanese black pine wood. Leptographium koreanum, L. pini-densiflorae, Ophiostoma ips, Penicillium raistrick, Trichoderma sp. were isolated from Korean pine wood. O. ips and L. koreanum were the major species on the two different PWN-infected pine tree. The cultivation of PWN on fungal mat of the identified species did some enhance PWN reproduction. The ambrosia beetle, Platypus koryoensis, is a serious pest of oak trees in Korea. In this study we investigated filamentous fungi present in the body of the beetle. Fourteen genera of filamentous fungi belonging to Ascomycota and Basidiomycota were isolated. All the obtained genera were isolated in the mitosporic state. The identified fungi were classified in 11 distinct orders including the Ascomycota (Eurotiales, Hypocreales, Microascales, Ophiostomatales, Pleosporales, and Sordiales) and Basidiomycota (Agaricales, Corticiales, Polyporales, and Russulales Xylariales). Within Ascomycota, 13 species were found. Meanwhile five species were found within Basidiomycota. The results showed the presence of diverse fungi in P. koryoensis. Among the isolated fungi, some were able to produce wood degrading enzymes. Further fungal isolation was performed with P. koryoensis infested Quercus mongolica trees sampled at Kumdan mountain in Hanam-Si, Gyeonggi province from June of 2009 to June of 2010. Penicillin spp. and Trichoderma spp. were the major species of mold fungi group. Pichia guilliermondii was the major species of mold yeast group. Raffaelea quercus-mongolicae was also isolated, but its isolation frequency was not high. Other species identified were Ambrosiella xylebori, Fusarium solani, Cryphonectria nitschke, Chaetomium globosum, and Gliocladium viride, Candida kashinagacola, C. maritima, C. vanderkliftii, Saccharomycopsis crataegensis.

• Journal of Bio-Environment Control
• /
• v.29 no.1
• /
• pp.96-102
• /
• 2020

Fusarium wilt caused by Fusarium oxysporum is a devastating disease limiting production of watermelon in Korea. The best way to control diseases is to use resistant gourd rootstock on watermelon. This study was conducted to establish an efficient screening method for resistant bottle gourd to Fusarium oxysporum f. sp. lagenaria. To develop an efficient inoculation method, incubation temperature after inoculation (15, 20, 25, and 30℃), inoculum concentration (1 × 10⁵, 5 × 10⁵, 1 × 10⁶, and 5 × 10⁶ conidia·mL^-1), and growth stages of seedlings (7, 10, 13, and 16 days) was investigated. Disease development of Fusarium wilt of bottle gourd was little affected by differences in incubation temperature and growth stages of seedlings. But resistant lines were more susceptible and appeared more severe symptoms at the higher inoculation level. Taken together, we suggest that an effective screening method for resistant gourd plant to Fusarium wilt is to dip the roots of 10-day old seedlings in spore suspension of 1 × 10⁵ - 1 × 10⁶ conidia·mL^-1, for 30 min, to transplant the seedlings into a non-infected soil, and then to incubate the inoculated plants in a growth room at 25℃ for 3 weeks to develop Fusarium wilt.

• Korean Journal of Environment and Ecology
• /
• v.23 no.4
• /
• pp.333-345
• /
• 2009

Carpinus laxiflora communities are known as the climax forest community of the temperate zone of Korea. There are three Carpinus laxiflora communities in Seoul. The purpose of this research is to analyze the characteristics of Carpinus laxiflora communities of Seoul and supply basic data for establishing of a management plan. The research sites are Sahmyook University, Mountain Dobong and Jinkwandong. This study considers investigation and the analysis of communities structure, soil characteristics and the growth increment of trees. As the result of study, we could know that the growth increment of the Carpinus laxiflora trees of Seoul is decreasing recently because of soil acidification and so on. Also the vegetation disturbance is increasing because of naturalized plant. Oak wilt disease has appeared in the oak forest around Sahmyook University that is the largest Carpinus laxiflora communities area in Seoul. For that reason the open gap was appeared in forest, and the spread of naturalized plants has been increasing in the gap. Furthermore, the Carpinus laxiflora trees are affected by the Platypus koryoensis which is a kind of oak wilt disease. And so, Unless there is a specific management plan, the survival of Carpinus laxiflora communities are uncertain in Seoul. Therefore the management plan of Carpinus laxiflora communities is necessary such as precise investigation, annual monitoring, etc. For the conservation of the Carpinus laxiflora communities, it is necessary to establish the management plan of the biodiversity for Seoul area.

• Korean journal of applied entomology
• /
• v.49 no.3
• /
• pp.211-218
• /
• 2010

Cuticular hydrocarbons (CHCs) of the pine sawyer (Monochamus saltuarius), Japanese pine sawyer (M. alternatus) and oak longicorn beetle (Moechotypa diphysis) were analyzed by GC, GC-MS and compared. Monochamus beetles are typical vectors of pine wilt disease but Moechotypa diphysis, which belongs to the same family, is not. They possess different CHCs in carbon number: 23-25 in M. saltuarius, 25-32 in M. alternatus, and 23-29 in M. diphysis. In comparison to inter-species, these three species of adult beetles have different numbers and chains of constituents of CHCs. In comparison between male and female in intra-species, the quantities of CHCs show the difference but constituents are not. Major constituent of M. saltuarius were analyzed as n-pentacosane > n-nonacosane > n-heptacosane; those of M. alternatus were n-nonacosene > n-pentacosane > n-nonacosane; and those of M. diphysis were n-heptacosane > 13-methylheptacosane > 3-methylheptacosane. From the body surface, most saturated carbohydrates of 3 species beetles are composed of n-alkane (40.2 - 65.7%) and followed by olefines > monomethylalkanes that one or two double bonds in M. saltuarius and M. alternatus. Otherwise, M. diphysis have the difference in order of monomethylalkanes > olefins.

• Research in Plant Disease
• /
• v.20 no.4
• /
• pp.270-274
• /
• 2014

Division of Forest Diseases & Insect Pests, Korea Forest Research Institute, Seoul 130-712, Korea The objective of this study was to evaluate the virulence of geographically different isolates of oak wilt pathogen, Raffaelea quercus-mongolicae and other Raffaelea species. In this study, mature trees of Quercus mongolica were inoculated with the various isolates of Raffaelea spp. and their virulence was evaluated by measuring the extent of sapwood discoloration resulting from the inoculation. The average length of discolored sapwood in a lateral direction was longest in the trees inoculated with the isolates from Korea (8.69 cm) followed by R. quercivora (7.51 cm) and the other Raffaelea spp. (3.35 cm). The lateral length of discolored sapwood caused by the inoculation with Korean strains varied from 4.71 to 14.90 cm indicating their differences in virulence. The area of discolored sapwood caused by the inoculation with Raffaelea spp. varied from 1.57 to $8.42cm^2$ indicating their differences in virulence. Based on the length and area of the discolored sapwoods, isolated YY and wj43 appeared to have the highest virulence among all the Raffaelea isolates tested. Each of the two isolates was obtained from Gangwon Province and Jeonbuk Province, respectively.