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http://dx.doi.org/10.5423/RPD.2010.16.3.219

Genotypic Characterization of Oak Wilt Pathogen Raffaelea quercus-mongolicae and R. quercivora Strains  

Seo, Sang-Tae (Korea Forest Research Institute)
Kim, Kyung-Hee (Korea Forest Research Institute)
Lee, Sang-Hyun (Korea Forest Research Institute)
Kwon, Yong-Nam (Korea Forest Research Institute)
Shin, Chang-Hoon (Halla Arboretum)
Kim, Hye-Jeong (Dept. of Forest Environment Protection, Kangwon National University)
Lee, Sang-Yong (Dept. of Forest Environment Protection, Kangwon National University)
Publication Information
Research in Plant Disease / v.16, no.3, 2010 , pp. 219-223 More about this Journal
Abstract
Recently, the oak wilt diseases especially on Quercus mongolica, have been increasing in various districts of Korea. A collection of 38 strains of the oak wilt pathogen Raffaelea qeurcus-mongolicae and R. quecivora isolated from Quercus spp. in Korea and Japan was characterized by $\beta$-tubulin gene sequence and randomly amplified polymorphic DNA (RAPD) analysis. In cluster analysis based on $\beta$-tubulin gene sequence the strains were divided into 4 clusters, of which clusters 2 and 4 were composed of Japanese strains except for one Korean strain. RAPD analysis showed that they were also effectively differentiated by a strong RAPD fragments. On the basis of the two genetic analysis, significant differences were detected between Korean strains and Japanese strains.
Keywords
Raffaelea qeurcus-mongolicae; R. quecivora; RAPD; $\beta$-tubulin;
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