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EVALUATING TWO METHODS FOR FINGERPRINTING GENOMES FOR STREPTOCOCCUS MUTANS IN CHILDREN : A COMPARISON WITH AP-PCR AND SOUTHERN BLOT RFLP (유전자형에 따른 Streptococcus mutans의 subtyping: Southern blot RFLP와 AP-PCR을 이용한 비교)

  • Jeong, Tae-Sung;Kim, Shin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.25 no.2
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    • pp.292-303
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    • 1998
  • The arbitrary primer polymerase chain reaction(AP-PCR) and Southern blot restriction fragment length polymorphism(RFLP) were used to genotype the cariogenic pathogen S. mutans in children. Following the morphologic chracteristics of colony on selective medium for S. mutans, total genomic DNA from 155 strains was extracted by conventional methods. Among 155 strains, 143 strains (92.3%) were confirmed S. mutans by PCR with dexA gene and 114 strains were used in this study. Three random sequence 10-base oligonucleotide primers were chosen for AP-PCR. The amplified DNA products were separated electrophoretically in a 2% agarose gel containing ethidium bromide and the banding patterns were compared among different strains. For RFLP analysis, DNA was digested with EcoRI and BamHI, separated on a 0.7 % agarose gel and transferred to a nylon membrane. The membrane was probed with a previously characterised 1.6 kilobases (kb) DNA fragment cloned from gtf B gene of S. mutans. The probe was labeled with isotope[$^{32}P-{\alpha}CTP$], and hybridized fragments were detected with intensifying screen. AP-PCR produced 4-8 DNA bands in the 0.25-10 kb regions and distinguished 9, 10 or 12 genotypes, depending on the specific primer used. Southern blot RFLP analysis revealed 2 hybridization patterns consisting of 1 DNA fragments 450, 500 bp. These results indicate that AP-PCR is more discriminative method for genotyping of S. mutans.

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화학복필름접착을 위한 최적화된 플라즈마 조건 확립

  • Park, Pyeong-Gyu;Choe, Yeong-Deok;Kim, Ui-Yong;Go, Jae-Seon;Yun, Byeong-Seon
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2009.05a
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    • pp.59.2-59.2
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    • 2009
  • 화학보호복은 독성이 있는 화학물질 및 미세분진등에 대해 공기를 차단하며 완전 밀폐형으로 공기호흡기 및 에어라인 같은 호흡보호장치와 함께 착용하여 신체부위를 보호한다. 그 예로 생물/화학보호복은 유독하고 해로운 생물/화학물질로 부터 인체를 보호해준다. 이들 보호복은 다양한 환경이 노출되어 장시간 작업을 위해서 오랜시간 보호성능을 유지해야한다. 특히, 이런 원단의 구성은 플라스틱과 고무류의 다층구조로 구성되어있다. 플라스틱류(폴리에틸렌, PTFE 등)는 표면장력이 너무 낮아 접착하는데 어려운 점이 많이 대두된다. 일반적인 표면처리방법은 크게 물리화학적 방법으로 4가지로 분류한다. 화학적산화, 불꽃처리, 플라즈마처리, UV 방사법 등이 있다. 이들 중에서 가장 간단한 산화처리는 플라즈마처리다. 이처리는 상온/상압하에서 대기 중 또는 가스내에 방전에 의해 플라즈마를 형성하고 이 플라즈마가 대상물의 표면분자와 격렬히 반응하게 하여 표면의 분자구조를 변화시킴에 따라 소수성의 표면에 Carboxyl, hydroxyl과 carbonyl과 같은 친수성으로 변하여 결합능력을 증가 시켜 표면장력을 높여주는 가장 효과적인 방법이다. 플라즈마 표면처리를 하고 나면 육안으로 표면의 변화를 감지할 수 없지만 접착, 잉크, 코팅을 잘 받아들이는 결과를 가져온다. 플라즈마 표면처리의 효과는 주로 부도체의 필름이나 합성수지 계열의 인쇄성과 접착성을 향상시키고자 많이 활용되고 있는 실정이다. 특히, 화학보호복과 같은 플라스틱류인 다양한 고분자 합성수지(Polyethylene, polypropylene, nylon, vinyl, PVC, PET 등)에 적용가능하다. 본 연구에서는 플라즈마처리조건에 영향을 주는 변수들을 고려하여 실험계획법(DOE, RSM)을 이용하여 최적화된 플라즈마 공정을 향상시키고자한다.

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Study on the Applicability of the Air Cushion Material for Impact Relief through Thermal Bonding of High Strength Fabrics (고강력 직물의 열융착 라미네이팅을 통한 충격 완화용 에어쿠션 소재로의 적용 가능성 검토 연구)

  • Kim, Ji Yeon;Kim, Hun Min;Min, Mun Hong
    • Textile Coloration and Finishing
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    • v.32 no.3
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    • pp.176-183
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    • 2020
  • In order to study wearable air cushion materials capable of responding to massive impact in high-altitude fall situation, high tenacity woven fabrics were bonded by heat only depending on various type of thermoplastic films and then mechanical properties were measured. Tensile strength, elongation, and 100% modulus measurement results for 4 types of films show that TPU-2 has higher impact resistance and easier expansion than PET-1. After thermal bonding, the combination with the highest tensile strength was a material with a TPU-2 film for nylon and a PET-2 film for PET, so there was a difference by type of fabric. The tear strength of the bonded materials were increased compared to the fabric alone, which shows that durability against damage such as tearing can be obtained through film adhesion. All of the peel strengths exceeded the values required by automobile airbags by about 5 times, and the TPU-2 bonded fabric showed the highest value. The air permeability was 0 L/dm2 /min. For both the film and the bonded material, which means tightness between the fabric and the film through thermal bonding. It is expected to be applied as a wearable air cushion material by achieving a level of mechanical properties similar to or superior to that of automobile airbags through the method of bonding film and fabric by thermal bonding.

New Tie-over Dressing Method Using Hooks and Rubber Bands (갈고리와 고무밴드를 이용한 새로운 봉합고정드레싱법)

  • Kim, Gook-Jin;Lee, Youn-Jung;Kim, Nam-Gyun;Kim, Jun-Sik;Lee, Kyung-Suk
    • Archives of Plastic Surgery
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    • v.38 no.4
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    • pp.552-554
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    • 2011
  • Purpose: Though its general usage, traditional tie-over dressing using suture has a few drawbacks such as difficulty in re-fixation after its opening especially when hematoma or seroma has occurred. It is rather difficult to maintain a stable dressing on curvy parts of body like flank, buttocks and that leads to unsatisfactory results of the surgery. Authors recommend a quick and repeatedly doable method, tie-over dressing that incorporates the usage of hooks and rubber bands. Methods: Debridement was done at a recipient site to be able to do skin graft. A right size of skin graft was prepared and placed upon the defect site with suture. Enough number of hooks were attached using Blue nylon at the normal skin of the edge of grafted area. We Applied dressing with ointment and fluffy gauze then fixed the dressing by attaching a rubber band at the hook to give a certain amount of tension. One or two days after the surgery, we opened the tie-over dressing and repeated the tie-over dressing by reusing the hooks and rubber band. Results: The skin grafts were all successfully taken and by repeating tie-over dressing using hooks and rubber, we could take the advantage of shortened the dressing time and eliminate the inconvenience of the patient and the surgeon by using bandages and fixing tapes. Conclusion: The advantage of tie over dressing using hooks and rubber bsnds are its easy re-doability early detection of probable complications, preventability of reoccurence of hematoma and seroma. Therefore, authors report this as considering the tie-over dressing using hooks and rubber bands is recommendable.

Studies on the Utilization of Arkshell 1. Preparation and Quality Stability during Storage of Powdered Dried Arkshell for Instant Soup (피조개의 이용에 관한 연구 1. 피조개 분말수우프의 제조 및 저장중의 품질안정성)

  • Kim, Hee-Yun
    • Journal of Food Hygiene and Safety
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    • v.3 no.4
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    • pp.217-223
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    • 1988
  • For the Effective utilization of the fish resources in coastal regions, investigations on preparation of powdered dried arkshell instant soup., quality stability of the products during storage and utilization as a food material were carried out with arkshell, Anadara broughtonii. Three kinds of powdered instant soup were prespared as 0% table salt(A), 5% table salt (B), 15% table salt(C) and packed with vacuum in laminated film bag. (polyester/nylon: 85${mu}ell$/15${mu}ell$, 18$\times$27cm) Their processing conditions and quality stability during storage at room temperature for 90 days were examined. Powdered instant soup was made by washing raw arkshell to remove visceral, clay, sand and blood, hot air drying(60$\pm$1$^{\circ}C$, 20 hrs) after draining and pulverizing dried arkshell to 35 mesh. Powdered instant soup was made by adding 2% sugar 0% table salt (5% and 15% table salt), 10.5% monosodium glutamate, 0.3% black pepper and 0.3% garlic powder to the pulverized dried arkshell. The condition of moisture and water activity of the products were 5.9-6.9% and 0.42-0.43, respectively. The moisture content s , water activity and pH of the products were showed little change and volatile basic nitrogen of them increased slightly during storage. Thiobarbituric acid value increased up to 60 days of storage and then decreased slightly. In solubility, powdered instant soup were showed no remarkable difference comparing with goods on the market. The color value of th products were showed little change during storage, In sensory evaluation, product B were scored slightly higher, in most cased, in flaver, color. taste and overall acceptability comparing with product A or product C during storage. Judiging form the sensory evaluation, powdered instant soup of 5% table salt (B) were the most desirable, and the quality of the products was stable for 90 days at 20$\pm$3$^{\circ}C$.

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Microbiological and Physicochemical Quality of Irradiated Ground Beef as Affected by Added Garlic or Onion

  • Rico, Catherine W.;Kim, Gui-Ran;Jo, Cheo-Run;Nam, Ki-Chang;Kang, Ho-Jin;Ahn, Dong-Uk;Kwon, Joong-Ho
    • Food Science of Animal Resources
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    • v.29 no.6
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    • pp.680-684
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    • 2009
  • The effects of garlic and onion on the microbiological and physicochemical properties of irradiated ground beef patties were evaluated. Ground beef was mixed with 0.5%(w/w) minced garlic or 2.5%(w/w) minced onion, vacuum-packed in oxygen-impermeable nylon/PE bags and then electron beam-irradiated at 2.5 kGy. All samples were kept at $4^{\circ}C$ for 8 d. Irradiation resulted in a 2-log CFU/g reduction in both aerobic and coliform bacteria. The microbial counts gradually increased during storage, but those in beef that contained garlic or onion were 1-log CFU/g lower than those of the control samples after 8 d of storage. The pH value decreased during storage in all meat samples, but this decrease was greater in non-irradiated beef than in irradiated beef. The lipid oxidation, volatile basic nitrogen content and Hunter color values of raw patties were generally not affected by irradiation or the addition of garlic and onion. Sensory evaluation of cooked patties showed that the off-odor was less pronounced in samples that contained added garlic or onion than in control samples, and that the overall acceptability of beef formulated with garlic was the highest. Overall, the results of this study indicate that the addition of garlic or onion in combination with irradiation treatment enhanced the microbial quality and improved the sensory quality of irradiated ground beef.

Analyzing Drift Patterns of Spray Booms with Different Nozzle Types and Working Pressures in Wind Tunnel (풍동실험에 의한 붐식 살포 농약의 노즐형태와 분사압력에 따른 비산 특성 분석)

  • Park, Jinseon;Lee, Se-Yeon;Choi, Lak-Yeong;Jeong, Hanna;Noh, Hyun Ho;Yu, Seung-Hwa;Song, Hosung;Hong, Se-woon
    • Journal of The Korean Society of Agricultural Engineers
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    • v.63 no.5
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    • pp.39-47
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    • 2021
  • With rising concerns about pesticide spray drifts, this study analyzed the drift patterns of two typically-used nozzles, XR nozzle and AI nozzle, concerning their working pressures and wind speeds by wind tunnel experiments. AI nozzle showed low drift potential with larger droplet sizes compared to XR nozzle. Airborne and deposition drifts of XR nozzle were two times higher than those of AI nozzle under high wind speeds (≥2 m s-1). In all cases, higher working pressures decreased the droplet sizes, thereby increasing the airborne and deposition drifts. Higher wind speeds also resulted in more airborne drifts, while ground deposition was increased under lower wind speeds. These effects of working pressures and wind speeds on the airborne and deposition drifts were observed at leeward distances less than 4 m from the nozzles. However, the airborne and deposition drifts were barely affected by the working pressures and wind speeds at leeward distances more than 11 m. The measurements were fitted to regression models of the drift curve with acceptable R2 values greater than 0.8, demonstrating that further studies will be useful to settle domestic issues of spray drifts.

Capillarity-Driven Self-Assembly of Silver Nanowires-Coated Fibers for Flexible and Stretchable Conductor

  • Li, Yi;Chen, Jun;Han, Xiao;Li, Yinghui;Zhang, Ziqiang;Ma, Yanwen
    • Nano
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    • v.13 no.12
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    • pp.1850146.1-1850146.9
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    • 2018
  • The rapid development of smart textiles requires the large-scale fabrication of conductive fibers. In this study, we develop a simple, scalable and low-cost capillary-driven self-assembly method to prepare conductive fibers with uniform morphology, high conductivity and good mechanical strength. Fiber-shaped flexible and stretchable conductors are obtained by coating highly conductive and flexible silver nanowires (Ag NWs) on the surfaces of yarn and PDMS fibers through evaporation-induced flow and capillary-driven self-assembly, which is proven by the in situ optical microscopic observation. The density of Ag NWs and linear resistance of the conductive fibers could be regulated by tuning the assembly cycles. A linear resistance of $1.4{\Omega}/cm$ could be achieved for the Ag NWs-coated nylon, which increases only 8% after 200 bending cycle, demonstrating high flexibility and mechanical stability. The flexible and stretchable conductive fibers have great potential for the application in wearable devices.

The Effect of Manufacturing Conditions of Coated Yarn Using Anti-Static Thermoplastic Polyurethane M/B on Anti-Static Resistance (대전방지 열가소성폴리우레탄 M/B를 이용한 코팅사 제조 조건이 대전방지성에 미치는 영향)

  • Yedam Jeong;Jieun Kwon;Sunmin Kwon;Seehyeon Chae;Hyunjea Cho;Wooseok Kim;Mikyung Kim;Jongwon Kim
    • Textile Coloration and Finishing
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    • v.35 no.1
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    • pp.20-28
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    • 2023
  • In this study, TPU resin for coating was prepared by varying the mixing ratio of antistatic TPU and recycled TPU to manufacture permanent antistatic materials. The coated yarn was prepared by coating on the nylon yarn, and then the thermal, rheological, mechanical properties and antistatic properties were analyzed. In addition, antistatic properties and durability were confirmed after manufacturing UD fabrics using coated yarns. The mixing ratio of antistatic TPU and recycled TPU was most appropriate at 4:6, and the antistatic property had a surface resistance of 2.20 × 109 Ω and a static charge of 398 V. In the coating process, the coating speed was most appropriate at 0.21 m/s, and the surface resistance of the UD fabric manufactured with the coated yarn manufactured under this condition was 6.80 × 109 Ω and the static charge was 484 V. The UD fabric had a surface resistance of 7.21 × 109 Ω and a static charge of 517 V after washing 10 times, and it was confirmed that the permanent antistatic property was excellent.

Magneto-Mechano-Triboelectric Generator Enabled by Ferromagnetic-Ferroelectric Composite (강자성-강유전성 복합체를 활용한 자기-기계-마찰전기 변환 발전소자)

  • Yeseul Lim;Geon-Tae Hwang
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.37 no.1
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    • pp.112-117
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    • 2024
  • The Internet of Things (IoT) device is a key component for Industry 4.0, which is the network in homes, factories, buildings, and infrastructures to monitor and control the systems. To demonstrate the IoT network, batteries are widely utilized as power sources, and the batteries inevitably require repeated replacement due to their limited capacity. Magneto-mechano-electric (MME) generators are one of the candidate to develop self-powered IoT systems since MME generators can harvest electricity from stray alternating current (AC) magnetic fields arising from electric power cables. Herein, we report a magneto-mechano-triboelectric generator enabled by a ferromagnetic-ferroelectric composite. In the triboelectric nylon matrix, a ferromagnetic carbonyl iron powder (CIP) was introduced to induce magnetic force near the AC magnetic field for MME harvesting. Additionally, a ferroelectric ceramic powder was also added to the MME composite material to enhance the charge-trapping capability during triboelectric harvesting. The final ferromagnetic-ferroelectric composite-based MME triboelectric harvester can generate an open-circuit voltage and a short-circuit current of 110 V and 8 μA, respectively, which were enough to turn on a light emitting diode (LED) and charge a capacitor. These results verify the feasibility of the MME triboelectric generator for not only harvesting electricity from an AC magnetic field but also for various self-powered IoT applications.