We investigated egg collection and the effects of water temperature (4, 7, 10, 13 and $16^{\circ}C$) on egg development, hatching and larval growth of Pacific cod Gadus macrocephalus under laboratory conditions. Fertilized eggs were round in shape ($1.01{\pm}0.03\;cm$) and adhesive to nylon nets. Fertilization rate was 68% by wet method. The time of egg development was negatively proportional to water temperature with the range of $4^{\circ}C$ to $13^{\circ}C$. Eggs hatched only at $7^{\circ}C$ after 288 hours of fertilization and $10^{\circ}C$ after 192 hours. Hatching rate was highest as 65% at $7^{\circ}C$ followed by $34.4^{\circ}C$ at $10^{\circ}C$. Survival rate was 18.3% at $7^{\circ}C$ and 5.2% at $10^{\circ}C$.
This study aimed to evaluate effects of 800 W microwave irradiation for 2, 4 and 6 min on chemical composition, antinutritional factors, ruminal dry matter (DM) and crude protein (CP) degradability, and in vitro CP digestibility of canola seed (CS). Nylon bags of untreated or irradiated CS were suspended in the rumen of three bulls from 0 to 48 h. Protein subfractions of untreated and microwave irradiated CS before and after incubation in the rumen were monitored by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Microwave irradiation had no effect on chemical composition of CS (p>0.05). There was a linear decrease (p<0.001) in the phytic acid and glucosinolate contents of CS as irradiation time increased. Microwave irradiation for 2, 4 and 6 min decreased the phytic acid content of CS by 8.2, 27.6 and 48.6%, respectively. The total glucosinolate contents of CS microwave irradiated for 2, 4 and 6 min decreased by 41.5, 54.7 and 59.0% respectively, compared to untreated samples. The washout fractions of DM and CP and degradation rate of the b fraction of CP decreased linearly (p<0.001) as irradiation time increased. Microwave irradiation for 2, 4 and 6 min decreased effective degradability (ED) of CP at a ruminal outflow rate of 0.05 $h^{-1}$ by 4.7, 12.3 and 21.0%, respectively. Microwave irradiation increased linearly (p<0.001) in vitro CP digestibility of ruminally undegraded CS collected after 16 h incubation. Electrophoresis results showed that napin subunits of untreated CS disappeared completely within the zero incubation period, whereas cruciferin subunits were degraded in the middle of the incubation period (16 h incubation period). In 4 and 6 min microwave irradiated CS, napin subunits were degraded after 4 and 16 h incubation periods, respectively, and cruciferin subunits were not degraded untile 24 h of incubation. In conclusion, it seems that microwave irradiation not only protected CP of CS from ruminal degradation, but also increased in vitro digestibility of CP. Moreover, microwave irradiation was effective in reducing glucosinolate and phytic acid contents of CS.
Kim, Ji Young;Yoon, Eun Kyung;Kim, Jong Soo;Seong, Nu Ri;Yun, Sang Soon;Jung, Yong Hyun;Oh, Jae Ho;Kim, Hyochin
Korean Journal of Environmental Agriculture
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v.38
no.4
/
pp.321-331
/
2019
BACKGROUND: Pinoxaden is the phenylpyrazoline herbicide developed by Syngenta Crop Protection, Inc. and marketed on 2006. The maximum residue levels for wheat and barley were set by import tolerance. Thus, Ministry of Food and Drug Safety (MFDS) official analytical method determining Pinoxaden residue was necessary in various food matrixes. Satisfaction of international guideline of CODEX (Codex Alimentarius Commission CAC/GL 40) and National Institute of Food and Drug Safety Evaluation-MFDS (2017) are additional pre-requirements for analytical method. In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was investigated to analyze residue of Pinoxaden (M4), which is defined as pesticide residue in Korea, in foods. METHODS AND RESULTS: Pinoxaden (M4) was extracted followed by acid digestion (2hr reflux with 1N HCl) and pH adjusting (pH 4-5 with 3% ammonium solution). To remove oil, additional clean-up step with hexane saturated with acetonitrile was required to high oil contained sample before purification. HLB cartridge and nylon syringe filter were used for purification. Then, samples were analyzed by LC-MS/MS using reserve phase column C18. Five agricultural group representative commodities (mandarin, potato, soybean, hulled rice, and red pepper) were used to verify the method in this study. The liner matrix-matched calibration curves were confirmed with coefficient of determination (r2) > 0.99 at calibration range 0.002-0.2 mg/kg. The limits of detection and quantitation were 0.004 and 0.01 mg/kg, respectively, which were suitable to apply Positive List System (PLS). Mean average accuracies of pinoxaden (M4) were shown to be 74.0-105.7%. The precision of pinoxaden and its metabolites were also shown less than 14.5% for all five samples. CONCLUSION: The method investigated in this study was suitable to CODEX (CAC/GL 40) and National Institute of Food and Drug Safety Evaluation-MFDS (2017) guideline for residue analysis. Thus, this method can be useful for determining the residue in various food matrixes in routine analysis.
Lee, Bo Eun;Jeon, Young Joon;Jang, You Lim;Kim, Jae Geun
Journal of Wetlands Research
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v.17
no.2
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pp.203-208
/
2015
To identify the effect of fallen cherry blossom on the artificial pond ecosystem, microcosm experiment was conducted into the aquatic decomposition of Prunus species petals. Petals were put in $1mm^2$ mesh nylon litter bags. For treatment group, one flower litter bag was placed into each pot microcosm ($27{\times}20{\times}8cm^3$) filled with influent water from the artificial pond, whereas control group microcosm contained pond water only. Decomposition time were set differently (4, 8, 12, 16 days) among treatment groups. At the end of experiment, most petals were decomposed and only 32.3% of initial dry weight remained with the decay rate (k) of $7.06{\times}10^{-2}day^{-1}$. $NO_3-N$ concentration of microcosm water decreased sharply from 1.90 mg/L at first to 0.02 mg/L, whereas $NH_4-N$ concentration increased from 0.03 mg/L to 2.85 mg/L continually. $PO_4-P$ concentration was 0.03 mg/L at first and increased to 2.39 mg/L by decomposition. Therefore, available phosphorus seems to have leached with higher rate than nitrogen from the petals litter. Increase about 0.02 mg/L in $PO_4-P$ concentration could be estimated in artificial pond from the calculation on the total quantity of fallen blossoms. This result suggests that available phosphorus from the decomposed Prunus petals could cause eutrophication in the artificial pond.
This study was conducted to clarify the effects of the Korean raisin (Hovenia dulcis Thunb) extracts and polyherbal extracts consisted of 6 types herbal extracts including HDT (HDTmix) on $CCl_4$ induced organ damages. Extracts were prepared by autoclave ($121^{\circ}C$, 15 psi, 3 hours) and filtered with nylon cloth and filter paper then freezing dried. Male Sprague-Dawley rats $(200^{\circ}{\ae}20g)$ were used as experimental groups, which were divided into 5 groups; intact control group (100 mg/kg), $CCl_4$ dosing group (100 mg/kg), $CCl_4$ dosing after HDT extract dosing group (100 mg/kg), $CCl_4$ dosing after HDTmix dosing group (100 mg/kg), Silymarin dosing group (5 mg/kg) after all test articles were orally dosed once a day for 28 days. They were sacrifiled under ether anesthesia. HDT extracts and HDTmix dramatically inhibits the $CCl_4$ intoxicated hepato/nephropathies with immuno-suppress changes on the spleen. They showed more dramatical protective effects on most of specific organs compared to that of Silymarin 5 mg/kg except for hepatoprotective effects in which, quite similar effects were detected. In addition, HDT extracts showed synergic effects with other types of herbal extracts because HDTmix showed more favorable protective effects on the all specific organs showing $CCl_4-related$ histopathological changes compared to HDT extracts.
A series of conductive filler were prepared with electroless plating method. Base conductive materials of the filler were nickel and copper. The cores were prepared with Nylon 6 and rayon in different aspect ratio. Also, various complexes were made with ABS resin and conductive filler with different filler feed ratio. The conductivity of the filler was measured with conductivity analyzer and the size distributions of fillers was measured with laser particle size analyzer. Electromagnetic wave shielding efficiency of each complex film was measured with flange circular coaxial transmission line sample holder within the 1MHz$\sim$1GHz bandwidth range. From this study, the conductivity of filers surpass that of other carbon films. It is available that the filler made of fibrous materials can be applied in plastic molding industry of electric appliances as a EMI filler.
The purpose of this study was to investigate the thermal resistance and the liquid/vapor water transfer characteristics of four waterproof and water vapor permeable coated fabrics with the ground fabric called nylon taffeta. In order to establish the experimental environment, outdoor temperature and humidity in Taegu during the last three years were examined and the experiment was performed at (1) $15^{\circ}C$, 50% R.H., (2) $20^{\circ}C$, 60% R.H., (3) $25^{\circ}C$, 65% R.H., which were the average standards in spring and fall. The test results were as follows ; 1. Among physical parameters, the thinner the thickness was, the higher the water vapor permeability was. But the porosity in thickness was not proportional to water vapor permeability linearly. 2. The thicker the thickness of specimens was and the smaller the bulk density and porosity were, the higher the thermal resistance. And the results also shown that the larger the temperature difference between the environmental temperature and the hot plate was, the more the difference of CLO values was apparent. 3. Since the contact angle of all specimens are above $90^{\circ}$, the all specimens have a good performance in waterproof. The more the specimens surface were rough, the higher the thermal resistance was. 4. According to the result of performing moisture transfer test using the simulating body skin-clothing-environment system, the humidity sensor placed in between the fabric and the environment detected the full saturation in 10 minutes after the experiment had began at $15^{\circ}C$, 50% R.H. and in 15 minutes after the experiment both at $20^{\circ}C$, 60% R.H. and at $25^{\circ}C$, 65% R.H. 5. ${\Delta}$ values of the humidity sensors placed in between the human body and the fabric and in between the fabric and the environment fluctuated repeatedly within the range of $20{\sim}40%$ at $20^{\circ}C$, 60% R.H., and $15{\sim}30%$ at $25^{\circ}C$, 65% R.H.
Park, kwanho;Kim, Wontae;Kim, Eunsun;Choi, Ji-Young;Kim, Sung-Hyun
International Journal of Industrial Entomology and Biomaterials
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v.33
no.2
/
pp.92-95
/
2016
The black soldier fly is economically important because its prepupae are used as feed for many animals, including fish and swine. In Korea, black-soldier-fly farms have attempted to increase annual breeding and mass egg production for use in animal feed, as well as the decomposition of organic waste. Such efforts require an understanding of optimal mating and oviposition techniques. Specifically, adult densities and cage size may both improve the efficiency of mass egg production. Our study used four sizes of nylon cages ($1.0{\times}1.0{\times}2.5m$, $1.5{\times}1.5{\times}2.5m$, $2.0{\times}2.0{\times}2.5m$, $2.5{\times}2.5{\times}2.5m$) and three density treatments (4 kg, 8 kg, and 10 kg of pupae) to investigate optimal habitat size and adult density. We found that cage size (independent of density) did not significantly influence female fecundity (number of egg clutches and egg weight), whereas higher densities increased egg number and weight regardless of cage size. Thus, we recommend manipulating adult density to enhance productivity in commercial black-soldier-fly farming. However, we also propose further detailed research to develop methods that account for seasonal changes and environmental conditions, as climatic variables (temperature, sunlight) likely influence female fecundity as well.
Journal of the Korea Fashion and Costume Design Association
/
v.18
no.1
/
pp.169-181
/
2016
This study analyzed the characteristics and patterns of men's drawers panties to get basic data for their design. For this, drawers panties in 7 different brands (4: domestic brands, 3: imported brands) in terms of a front-center shape were collected, and the shape of men's drawers panties, characteristics of the materials, dimensions & type of patterns by the part and front-center patterns were analyzed. The study results found the followings: First, in terms of the shape of men's drawers panties, the front-center part were designed to rest the penis and scrotum in a natural and comfortable manner without a pee slit. They were seamless on the sides except for one product with side seams. Second, the drawer panties were made of elastic polyurethane materials. Specifically, domestic brand items used warm, elastic and durable nylon, polyester and rayon and polyurethanes while imported brands were mostly made of absorbent and sanitary polyurethanes. Third, regarding a size of drawer panties by part, imported brands were large in waist circumference. In terms of waist breadth at the front center, there was difference depending on an oval type. In addition, the front length at the front center differed by product, revealing difference in three-dimensional effect at the front center. The side length also differed greatly by product, which means that there might be a great difference in wear comfort. Fourth, in terms of the patterns of men's drawers panties, there was difference in side seams, center back seam and front-center patterns. Among a total of 7 products, only one product had side seams, and center back seams were found in two products only. Fifth, in terms of front-center patterns, the drawer panties can be divided into three types, creating three-dimensional effect not to give any pressure on the crotch: i) the cutting line only at the bottom of the front center part where the penis is rested, ii) puff created at the front center by adding a transverse line to the center line, iii) cutting transverse line to separate the penis from the testicles.
Kim, Sug-Won;Chung, Yoon-Kyu;Kang, Sang-Yoon;Cho, Pil-Dong
Archives of Reconstructive Microsurgery
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v.10
no.1
/
pp.12-17
/
2001
Recovery of nerve injury is conditioned by various factors including physical state, injured site, cause of injury, and neurorrhaphy Many researchers have reported on regeneration of nerve using end to side neurorrhaphy. The purpose of this study was to examine regeneration of nerve in various conditioned side to side neurorrhaphy. Total of 25 male Sprague-Dawley rats weighing 220 to 250 gm were divided into five groups of five rats each. The group 1, sham group, composed of dissection only without nerve transaction. The group 2, control group, composed of nerve division only without neurorrhaphy or sural nerve graft. The group 3 composed of one segmental sural nerve graft between the tibial and peroneal nerve after division. Group 4 had two segment graft, and the group 5 with three segment graft, each segment being 6mm long and 5 mm apart. The side to side neurorrhaphy was performed between peroneal nerve and tibial nerve using segmental sural nerve graft in rats. We exposed the sciatic nerve, tibial nerve, peroneal nerve, and sural nerve on left side with prone position. The peroneal nerve was cut on the bifurcation site from tibial nerve and the side to side epineurial neurorrhaphy was performed between peroneal nerve and tibial nerve through 6 mm sural nerve segment graft with 11-0 nylon under operating microscope. The electromyography and the weight from ipsilateral tibialis anterior muscle was performed at one month after neurorrhaphy Peroneal and tibial nerve was examined at distal and proximal to the neurorrhaphy site by methylene blue stain under light microscope for histologic appearance. The number of nerve fibers were counted using the image analyzer. Statistically, both in electromyography and number of nerve fibers, the differences in values between the groups were significant.
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