• Title/Summary/Keyword: nutrients components

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Proteases and Protease Inhibitors Produced in Streptomycetes and Their Roles in Morphological Differentiation

  • KIM DAE WI;KANG SUNG GYUN;KIM IN SEOP;LEE BYONG KYU;RHO YONG TAIK;LEE KYE JOON
    • Journal of Microbiology and Biotechnology
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    • v.16 no.1
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    • pp.5-14
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    • 2006
  • Streptomycetes are Gram-positive microorganisms producing secondary metabolites through unique physiological differentiation [4]. The microbes show unusual morphological differentiation to form substrate mycelia, aerial mycelia, and arthrospores on solid medium [19]. Substrate mycelium growth is sustaining with sufficient nutrients in the culture medium. The concentration of a specific individual substrate in the culture environment is the most important extracellular factor allowing vegetative mycelia growth, where extracellular hydrolytic enzymes participate in the utilization of waterinsoluble substrates. However, with starvation of nutrients in the culture medium, the vegetative mycelia differentiate to aerial mycelia and spores. It has been considered that shiftdown of essential nutrients for mycelia growth is the most important factor triggering morphological and physiological differentiation in Streptomyces spp. Since proteineous macromolecule compounds are the major cellular components, these are faced to endogenously metabolize following a severe depletion of nitrogen source in culture nutrients (Fig. 1). Various proteases were identified of which production was specifically related with the phase of mycelium growth and also morphological differentiation. The involvement of proteases and protease inhibitor is reviewed as a factor explaining the mycelium differentiation in Streptomyces spp.

Within- and Between-Individual Variation in Nutrient Intakes with Day of the Week and Season in Korean Adults (계절별, 요일별로 측정한 성인 남녀의 영양소 섭취량에서의 개인간 변이와 개인내 변이)

  • Kwon Eun Sil;Ahn YounJhin;Shim Jae Eun;Paik HeeYoung;Park Chan;Kimm Kuchan;Ju Young Su;Kim Dong Hyun
    • Journal of Nutrition and Health
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    • v.37 no.10
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    • pp.917-927
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    • 2004
  • This study was performed to examine component of variance in nutrient intakes and to estimate the level of accuracy with varying degree of precision in order to achieve estimates of usual nutrient intakes. Three-day dietary records including both weekdays and weekends were collected every 4 season over a I-year period from 36 males and 93 females aged 40 - 65 y. For each nutrient, we partitioned total intake variance into weekly (weekday vs weekend), seasonal, within- and between- individual variation as components of variance, using analysis of variance. It was found that major components of variance were within- and between-individual variation. Particularly, within-individual variation (57.2 - 87.1 %) was greater than between-individual variation (12.2 - 37.4%) for all nutrients. Weekly and seasonal variation contributed small components of variance for most nutrients. For protein, fat and carbohydrate, there were a little significant weekly variation (0.00 - 1.35%) in females but not in males. For some micronutrients, there were moderately significant seasonal variation (0.15 - 5.48%) in both sexes. Ratio of within- to between- individual variation ranged 1.4 (vitamin B$_2$) -4.5 (vitamin B$_1$) in males and 1.6 (carbohydrate) -2.9 (fat) in females. With total 12-day dietary records data, the maximum percentage deviation of observed intakes from usual (true) intakes ranged 12-37%. To estimate usual individual intakes within 20% of the true mean with 90% confidence level, 3 - 9 days of dietary survey were required for energy, protein, carbohydrate, phosphorus and iron, 13 - 19 days for fat and calcium, 25 - 29 days for vitamin A and vitamin C. Correlation coefficients between observed and true nutrient intakes were 0.71 - 0.91 for males, 0.81-0.91 for females. In conclusion, mean intakes of several nutrients can be reliably measured with the record method, using a limited number of days. Both nutrients of interest and the primary objectives should be taken account when planning method of assessment and number of replicates.

Study on the Establishment of Nutrient Requirements for Commercial Supplementary Foods for Infants and Young Children (국내 시판 이유식류의 영양성분규격 설정 방안)

  • 김동연;김경희;최혜미
    • Korean Journal of Community Nutrition
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    • v.2 no.4
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    • pp.624-632
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    • 1997
  • This study was conducted to evaluated the nutrition quality of the commercial supplementary foods for infants and young children and to seek a solution to the establishment of standards of nutrient requirements for supplementary foods in Korea. Information on food ingredients, nutrient contents, claims about usefulness of food components and instructions for feeding preparation were obtained from the labels of 33 commercial supplementary foods manufactured by 4 different domestic companies. According to the standard of supplementary foods for infants and young children described in the Korean Food Code, the commercial supplementary foods were categorized into two different types, weaning food and baby food. All the commercial weaning foods were in powder form and mainly composed of cereals, whereas all the baby foods were mainly composed of fruits in the form of canned juice. The weaning foods contained more nutrients than the baby foods did, and the nutrient levels of the weaning foods expressed as nutrient density on energy basis were higher than the RDA for infants aged 5 to 11 months, suggesting that the commercial weaning foods provide adequate amounts of nutrients. If one followed the instructions for feeding preparation appearing on the label, however, recommended amounts of intake of the weaning foods would provide too much energy as well as nutrients. There were many differences in nutrient standards of weaning foods between the Korean Food Code and Codex international food standard. In conclusion, the establishment of standards for nutrient requirements for the supplementary foods requires significant scientific studies on what nutrients are the most inadequate in Korean infants and young children feeds and what levels of nutrients should be added to the foods in order to supplement their nutrition. In addition, it is very important to have a strong scientific basis to support our standard when discrepancies exist between our standard and the international standard. (Korean J Community Nutrition 2(4) : 624-632, 1997)

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Effects of the Application of Different Fertilizers on the Forage Productivity and Quality on Newly Reclaimed Hilly Soil II. Changes in the contents and yields of mineral and general components (신개간 산지토양에서 초지조성 비종별 목초의 생산성 및 품질 비교 II, 목초 중 무기양분 및 일반성분의 함량과 양분수량)

  • 정연규;임요섭
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.21 no.1
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    • pp.7-14
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    • 2001
  • This pot experiments was conducted to find out the forage productivity and quality in a grass/clover sward as affected by the application of three different fertilizers; double superphosphate(DS), fused Mg-phosphate(FP), and complex fertilizer(CF) on newly reclaimed hilly soil. This 2nd part was concerned with the contents and yields of mineral nutrients and general components. The results obtained are summarized as follows: 1. The concentrations of P, Ca. Mg, and Na by the forage species were relatively higher in the CF plots than in the FP and DS plots. The yields of mineral nutrients by the forage species were high in the order: CF>FP>DS plots. Especially. these were higher in the ladino clover and mixed forages than in orchardgrass. 2 The contents of crude protein and crude ash were highest with the CF plots, compared with the FP and DS plots. The yields of general components in the ladino clover and mixed forages were apparently highest with the CF plots. whereas these in orchardgrass not showed consistent differences among the plots. It was recognized that the positive effects on the concentrations and yields of mineral nutrients and general components in forages were higher in the order: CF>FP>DS plots.

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A Study of the Nutritional Composition of the Dandelion by Part (Taraxacum officinale) (민들레의 부위별 영양성분 함량 비교)

  • 이성현;박홍주;한귀정;조수묵;이승교
    • The Korean Journal of Community Living Science
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    • v.15 no.3
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    • pp.57-61
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    • 2004
  • Many studies have presented results about the antioxidative and antimicrobial activities of Dandelions (Taraxacum officinale). There has yet to be a study which makes comparisons of nutrients based on the parts of the Dandelion. To identify the nutrient composition by part of dandelion, nutrient contents were analyzed. Dandelions were taken from Songpa-gu in Seoul and the nutrient composition of the flower, leaf and root were measured. The nutrient content of each part was analysed by using the method developed by the Association of Official Analytical Chemists (AOAC). The proximate components(moisture, protein, fat, fiber, ash, and carbohydrates), minerals and vitamins of the dandelion were analysed. The nutrient composition of the dandelions showed many significant differences among the parts when the differences were determined by using Duncan's multiple range test. The leaf contains more protein, ash, Ca, K, Mg, Zn, vitamin A, B$_1$, B$_2$, and C than the other parts. The root has much more fiber, carbohydrate, P and Fe content. The results demonstrate that dandelions could be used as a food source supplement for fiber, Ca, Fe and vitamin B$_2$ which are common nutritional deficiencies in Korea. It is recommended that more research for other bio-functional factors besides nutrients composition is needed to enhance the utilization of the dandelion.

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Sources of Variability of Multiple Self-reported 24-hour Recalls in Dietary Survey (식이조사방법중 자기 기록식 24시간 회상 반복 조사의 변이원에 관한 연구)

  • 김연희
    • Journal of Nutrition and Health
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    • v.30 no.3
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    • pp.349-359
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    • 1997
  • This study was designed to study sources of variability(subjects, day by day, season, day of week, sequence, number of repeated days, compliance etc.) in multiple 24-hour dietary recall method. Dietary intakes of college women in Seoul were obtained using 24-hour dietary recalls, consecutive 7 days, 4 times for 1 year. The result of this study shows that 1) the reproducibility of multiple 24-hour recalls is low(intraclass correlation coefficient<0.3), 2) the intraindividual variations of all nutrients are more than 80%, 3) the major components of variation are interindividual vriability and intraindividual variability(within person variability and methodologic error). There were small but statistically significant season and compliance effects. 4) the ratio within-person varation to between-person variation for absolute and transformed nutrient intakes except some nutrients such as vitamin A, vitamin C, energy from protein and fat etc, was less than 1.7 the minimum numbers of days needed in 24-hour dietary recalls to estimate usual intake for this group were 1 to 21 days. And the numbers of days needed to get the usual intake of an individual varied among individuals and within individuals for different nutrients, ranged from 1 days for log carbohydrate(% energy) to 470 days for Na. 6) There were greater than 0.7 correlation coefficients between the average nutrient intakes of 12 days and intakes of 28 days. Therefore, it was desirable to take the 24-hour recall more than 12 times, repeating 3 days every season.

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Time-dependent changes of fruit metabolites studied by 1H NMR

  • Park, Sung Jean
    • Journal of the Korean Magnetic Resonance Society
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    • v.26 no.3
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    • pp.24-33
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    • 2022
  • The browning phenomenon of fruits can be easily observed when fruits or vegetables (apples, pears, bananas, potatoes, etc.) are cut with a knife and the part turns brown. When this browning occurs, changes in taste, color, and nutrients usually are introduced. The cause of this browning phenomenon has been well studied for a long time, but these studies have mainly focused on preventing deterioration of processed foods during food processing or storage. Resultantly, there are few studies on how much changes in nutrients (saccharides, amino acids, fats, water-soluble low molecular weight ammonium ions, etc.) are caused by browning. The purpose of this study is to determine the change in nutrients during browning using apple as a model fruit. We conducted a comparative study on how much the nutrient fluctuations differ depending on the presence or absence of pretreatment such as the application of heat. All analysis was conducted using 1H NMR. The ANOVA analysis showed that the concentrations of 4 amino acids (alanine, asparagine, isoleucine, and valine), 3 types of sugars (fructose, glucose, and xylose), 1 type of organic acid (lactate) and choline were significantly increased in samples showing browning. In addition, the groups before and after browning were clearly separated using multivariate statistical analysis methods (PCA, PLS-DA), which was greatly contributed by two sugar components (fructose and glucose) present in high concentrations in apples.

Milk and Health of Elderly People (우유와 노인건강)

  • Chung, Un-Hyeon
    • Journal of Dairy Science and Biotechnology
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    • v.19 no.1
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    • pp.39-48
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    • 2001
  • As the development of medical technology and the elevation of the standard of living, the population rate of elderly people in Korea is increasing gradually. To keep a good lift quality of the elderly, both appropriate exercise and nutrients intake are necessary for them. Dairy products are known for the good source of variable nutrients including functional components and bioactive peptides such as Ig, lactoferrin, MFGM, OPP, CPP, GMP, sialic acid etc that are required especially for elderly people. However, they are classified as the low dairy products consumption group recently. For the promotion of dairy product consumption of elderly people, variable and specialized dairy products for the elderly should be researched and developed with the strengthened publicity activities.

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Television Food Advertisement: Review and Recommendation (텔레비전 식품 광고에 관한 고찰)

  • Kim, Hee-Sup
    • Journal of the Korean Society of Food Culture
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    • v.11 no.4
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    • pp.507-515
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    • 1996
  • Television food advertisement is the most effective way to reach to consumers with food and nutritional informations and affect their eating behavior. Therefore, 218 food commercials were reviewed using video tapes and copies to know the present food product trends, food messages they transmit and define misleading food commercials. Messages were focused on the benefit of health promoting substances they contain, especially for functional food components, fortified nutrients, food safety focused on food additives, convenience and differentiation with other products. Overnutrition on specific nutrients could be expected due to nutrient fortified products and misleading of food commercials were also noted. Regarding trends, guidelines provided by television broadcasting company shoud be fortified in the connection of Food Hygine Law and supervision committe should reinforce the food company to summit data for the approval of their advertisement claims. Nutrition educational spot program shoud be produced and broadcasted for the public to protect the consumer from food faddism in near future.

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Protein and Amino Acid Composition of Water Cress Oenanthe stolonifera DC (미나리의 단백질의 및 아미노산 조성)

  • 문숙임;조용계;류홍수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.19 no.2
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    • pp.133-142
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    • 1990
  • This study was attempted to compare the nutritive value of leaf with stem of the water cress Oenanthe stolonifera DC. in order to improve the eating habits and as a part of studying on the effective curing nutrients for the damaged liver. The contents of moisture crude proteinon the effective curing nutrients for the damaged liver. The contents of moisture crude protein crude fat and crude ash were 90.40% 2.85%, 0.42% and 0.74% in leaf while the contents of moisture crude protein crude fat and crude ash were 95.15% 0.77% 0.09%, and 0.64% of moisture crude protein crude fat and crude ash were 95.15% 0.77% 0.09% and 0.64% in stem respectively. The quantitative fractionation of proteini of both leaf and stem ranked albumin the highest content followed globulin prolamin and glutelin in order. It has been sh-own by SDS-polyacrylamide gel electrophoresis that water extractable protein of leaf 11 bands but those of stem were not detected. The scope of molecular weight for the main protein of water extractable protein of leaf was between 34.700 and 45,000. The amounts of extractive-nitrogen from leaf and stem of the water cress were 241.02mg% and 271.67mg% respec-tively. The amounts of free amino acid-nitrogen from the leaf and stem were 89.02mg% and 32.02mg% respectively. In free amino acid-nitrogen from the leaf and stem were 89.02mg% and 32.02mg% respectively. In free amino acid composition of both leaf and stem the major components were aspartic acid and glutamic acid. In total amino acid composition of water cress leaf aspartic and glutamic acid were the major components. Whereas alanine and thr-eonine were the major components in stem The assessment of water cress leaf and stem with chemical score. EAAl Rl showed that the values of stem were lower tendancy than those of leaf. Limiting amino acid of leaf was tryptophan while that of stem was lysine.

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