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Development of Efficient Screening Methods for Melon Plants Resistant to Fusarium oxysporum f. sp. melonis (멜론 덩굴쪼김병에 대한 효율적인 저항성 검정법 개발)

  • Lee, Won Jeong;Lee, Ji Hyun;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Heung Tae;Choi, Gyung Ja
    • Horticultural Science & Technology
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    • v.33 no.1
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    • pp.70-82
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    • 2015
  • This study was conducted to establish an efficient screening system to identify melon resistant to Fusarium oxysporum f. sp. melonis. F. oyxsporum f. sp. melonis GR was isolated from infected melon plants collected at Goryeong and identified as F. oxysporum f. sp. melonis based on morphological characteristics, molecular analyses, and host-specificity tests on cucurbits including melon, oriental melon, cucumber, and watermelon. In addition, the GR isolate was determined as race 1 based on resistance responses of melon differentials to the fungus. To select optimized medium for mass production of inoculum of F. oxysporum f. sp. melonis GR, six media were tested. The fungus produced the most spores (microconidia) in V8-juice broth. Resistance degrees to the GR isolate of 22 commercial melon cultivars and 6 rootstocks for melon plants were investigated. All tested rootstocks showed no symptoms of Fusarium wilt. Among the tested melon cultivars, only three cultivars were susceptible and the other cultivars displayed moderate to high resistance to the GR isolate. For further study, six melon cultivars (Redqueen, Summercool, Superseji, Asiapapaya, Eolukpapaya, and Asiahwanggeum) showing different degrees of resistance to the fungus were selected. The development of Fusarium wilt on the cultivars was tested according to several conditions such as plant growth stage, root wounding, dipping period of roots in spore suspension, inoculum concentration, and incubation temperature to develop the disease. On the basis of the test results, we suggest that an efficient screening method for melon plants resistant to F. oxysporum f. sp. melonis is to remove soil from roots of seven-day-old melon seedlings, to dip the seedlings without cutting in s pore s uspension of $3{\times}10^5conidia/mL$ for 30 min, to transplant the inoculated seedlings to plastic pots with horticulture nursery media, and then to cultivate the plants in a growth room at 25 to $28^{\circ}C$ for about 3 weeks with 12-hour light per day.

Methods that can be Substituted for Earth Healing of Seedling by Using the Plastic Vinyl and their Micro-climatical Characteristics (프라스틱비닐제품(製品)을 이용(利用)한 가식대치방법(假植代置方法)과 미기상학적(微氣象學的) 특성(特性))

  • Ma, Sang Kyu;Lee, Jang Soo
    • Journal of Korean Society of Forest Science
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    • v.47 no.1
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    • pp.44-51
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    • 1980
  • In order to find out the suitable methods that can omit the earth healing and increase the survival through improving the seedling-healing and transportable methods. Several trials with plastic vinyl have been done and its results are as follows: 1. Though P. rigitaeda seedling have been stored in the black and white vinyl sack for 35 days in the storehouse. This seedling have survived with very high percentage as Table 1. This means that the earth healing work at the nursery or planting area can be omitted if seedling sould be stored in the storehouse by using the vinyl sack. The possibilities of long-period storage in the black and white vinyl sack seem to be come from the reasons that air humidity in the sack is nearly 100% and its air temperature is only around $15^{\circ}C$ with very little difference between day and night time. This sack also can be utilized in place of the planting sack, and though this sack with seedling have been laid under direct sunshine for 1 to 2 days. Any difference between the sack stored in the storehouse has not been observed on the survival specially Table 2. 2. When the bundled seedling have been covered with the black and white vinyl instead of earth healing, even if these seedling have been laid for 18 days under the vinyl. This seedling show us high survival as Table 3. High humidity with nearly 95%, very little difference of air temperature between day and night time under the vinyl and not so big difference between out-and inside temperature could be reasons of high survival to be considered. So through covering by the black and white vinyl. The labour power for earth healing works can be saved also. 3. In order to protect the healed seedling from the direct sunshine and the eva-transpiration. Black vinyl net and reed mat could be effective for this purpose. Because vinyl net could intercept around one to third, reed mat two to third of total solar energy and also suppress more than 50% of total water loss by the transpiration.

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Effect of Fertilizer Component on Turfgrass Growth and Quality of Golf Putting Greens under Traffic Stress (답압하에서 질소, 인산, 칼륨 변화가 골프코스 그린 잔디의 생육과 품질에 미치는 영향)

  • Lee, Sung-Woo;Lee, Jae-Pil;Kim, Doo-Hwan
    • Asian Journal of Turfgrass Science
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    • v.21 no.2
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    • pp.137-146
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    • 2007
  • Traffic causes more and more stress and injury to grasses on golf course green in Korea due to the increased playing. This study compared the effect of fertilizer component (Nitrate, Phosphate, Potassium) on turfgrass growth and quality golf course green under traffic stress during early winter. Experiment was conducted by using different fertilizer components for 2 months(Oct. 1 to Nov. 30, 2005). Turfgrass leaf color, leaf texture, density and traffic tolerance were evaluated visually, and the root length(cm) and tiller density(tiller/$cm^2$) were measured. Creeping bentgrass(Agrostis palustris cv. 'Seaside II') fromthe nursery of Incheon Grand Golf Club was used. Results of this study are as followings: 1). Turfgrass color was the best in A6(20-15-10) and A5(15-15-10) treatments in the N study. Leaf texture was not different among treatments. Turf quality and traffic tolerance were the highest In A5 and A6. Root length was the longest(15.8cm) in A6 (20-15-10). Root length increased with nitrogen levels. 2). Turf color of A9(5-7.5-5) and A10(15-22.5-15) was darkest in the comparison of P study. Leaf texture was the best in A4(10-15-10) and A9. Turf quality was the best in A10. A7(10-0-10). 3). In general, to recover turfgrass damage on the putting greens during low temperature period, fertilizer amount need to be increased; and nitrogen is better than phosphate and potassium for that purpose.

Growth Characteristics and Visible Injury of Container Seedling of Pinus densiflora by Fertilization Level (시비수준별 소나무 용기묘의 생장 특성 및 가시적 피해)

  • Cha, Young Geun;Choi, Kyu Seong;Song, Ki Seon;Gu, Da-Eun;Lee, Ha-Na;Sung, Hwan In;Kim, Jong Jin
    • Journal of Bio-Environment Control
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    • v.28 no.1
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    • pp.66-77
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    • 2019
  • The present study investigated pine trees, which forms a major plantation species in Korea, with the objective of improving the survival rate of pine trees after planting. Growth responses and characteristics were assessed by controlling the level of fertilizer application, which is a basic controlling the growth of pine seedlings, to identify the optimal fertilization treatment. Pine tree seedlings were grown in 104 containers and were examined 8 weeks after planting. Stem height and were measured at 4-week intervals. In terms of fertilization treatment for 1-0 pine seedlings, the treatment group with gradually-increasing fertilizer concentration ($500{\rightarrow}1000{\rightarrow}1000{\rightarrow}1000mg{\cdot}L^{-1}$) had the biggest increase in stem height and diameter at the root. The survey results indicated that the increased concentration treatment group and the gradually-increasing concentration treatment group had more growth compared with that in the fixed concentration treatment group. The gradually-increasing concentration treatment group ($500{\rightarrow}1000{\rightarrow}1000{\rightarrow}1000mg{\cdot}L^{-1}$) had the highest total dry matter production. Nine weeks after fertilization, the tips of the pine leaves turned yellow in the fixed concentration treatment group ($3000mg{\cdot}L^{-1}$). The same phenomenon was observed in the treatment group in which the concentration was increased to $2000mg{\cdot}L^{-1}$, and in the gradually-increasing concentration treatment group, when the concentration was raised up to $2000mg{\cdot}L^{-1}$. We concluded that the optimal fertilization conditions for producing healthy pine 1-0 seedlings involve fertilizing once a week with Multifeed 19 at $500mg{\cdot}L^{-1}$ during the seedling period, Multifeed 19 at $1000mg{\cdot}L^{-1}$ during the rapid growth period, and Multifeed 32 at $1000mg{\cdot}L^{-1}$ during the maturation period.

A Case Study on Psychological Burnout and Self-care of Childcare Teachers for Emotional Labor -Song psychotherapy- (감정노동 보육교직원의 심리적 소진과 자기 돌봄의 관한 사례연구 -노래심리치료-)

  • Lee, Ji-Hoon;Shin, Soo-Won
    • Industry Promotion Research
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    • v.6 no.3
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    • pp.9-17
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    • 2021
  • Childcare teacher experience emotional labor that suppresses, exaggerates, controls and regulates their emotions in order to produce the positive image required in the nursery field. The working environment for infants and toddlers has a problem of lowering the quality of life as a cause of psychological exhaustion of the emotional labor childcare teacher. Because singing helps to improve the quality of human life, research is needed to enable emotional labor childcare teacher to recover from psychological exhaustion and plan a positive life in the process of self-care. First, how is the psychological exhaustion of the emotional labor childcare teacher through song psychotherapy? Second, how is the process of self-care of the emotional labor childcare teacher through song psychotherapy? The study was conducted from March 2017 to May 2020, and through qualitative case studies, data such as in-depth interviews, direct observations, and participation observations were collected at the ○○○ daycare center for 50 minutes every 12 sessions. Based on the above findings, the following conclusions were drawn: First, through singing psychotherapy, emotional labor and childcare staff were able to discover, understand, recognize, face, communicate, and insight into their will to live, psychologically exhausted themselves. Emotional support from others can reduce the experience of emotional depletion and demonstrate a recovery of experience and an improvement in achievement due to frustration at work. Second, the self-care of the emotional labor child care teacher through song psychotherapy proved the temporal, spatial, relational, and emotional caring process, while maintaining the balance between caring for others and caring for oneself, body, mind, and spirituality are organic change. In this study, the psychological exhaustion and self-care process provides an opportunity to discover the essence of life, explore and express one's inner self, take care of others and oneself in a balanced manner, and provide insights for a whole person and healthy self. It is significant in providing opportunities to improve the quality of life through growth.

Quality and Fruit Productivity of the Second Truss Blooming Seedlings Depending on Concentration of Nutrient Solution in Cherry Tomato (양액 농도에 따른 방울토마토 2화방 개화묘의 소질 및 과실 생산성)

  • Lee, Mun Haeng
    • Journal of Bio-Environment Control
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    • v.31 no.3
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    • pp.230-236
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    • 2022
  • This study was carried out to produce two-flowered seedlings, harvest them early in a greenhouse, and extend the harvest period. This study was carried out to effectively produce the second truss blooming seedlings to harvest tomatoes early and extend the harvest period. For production of the second truss blooming seedlings (one stem), the nutrient solution EC was supplied at 1.5, 2.0, 2.5 dS·m-1, and dynamic management (3.0 → 3.5 → 4.5 dS·m-1). The seedling period was 60 days, which was 20-40 days longer than conventional seedlings, and 10 days longer than the first truss blooming seedlings (cube seedlings). The plant height was 78 and 77 cm in EC 2.5 dS·m-1 and dynamic management respectively, which was shorter than EC 1.5 dS·m-1 with 88 cm. As for the EC in the cube before formulation, dynamic management had the highest EC 5.5 dS·m-1, and the cube supplied with EC 1.5 dS·m-1 had the lowest. The production yield by treatment did not a difference among in the second truss blooming seedlings, but the first truss blooming seedlings showed lower productivity than second truss blooming seedlings. The second truss blooming seedling were harvested 35 days after planting on June 4, the first harvest date, and the first truss blooming were harvested in 42 days on June 11th. There was no difference in plant height and root growth due to bending at frequency planting. In the study on the production of the second truss blooming seedlings (two stem), the nutrient solution EC was supplied under 2.0, 2.5, 3.0 dS·m-1, and dynamic management (3.0 → 3.5 → 4.5 dS·m-1). The seedling period was 90 days, which was 40-50 days longer than conventional seedlings and 10 days longer than the first truss blooming seedlings (cube seedlings). Plant height was 80 and 81 cm in EC 2.0 dS·m-1 and 2.5 dS·m-1 respectively, but was the shortest at 73 cm in dynamic management. EC in the medium increased as the seeding period increased in all treatments. The dynamic management was the highest with EC 5.1 dS·m-1. There was no difference in yield among EC treatments in the second truss blooming seedlings, which had a longer seeding period of about 10 days, produced 15% more than the first truss blooming seedlings. In order to shorten the plant height of the second truss blooming seedlings, it is judged that the most efficient method is increasing the concentration of nutrient solution.

The Induction of Somatic Embryogenic Callus from Petals-Derived Callus in Rosa hybrida (국내 육성 장미 품종 꽃잎 유래 체세포배 발생 캘러스 유도)

  • Lee, Su Young;Shin, Ju Young;Lee, Young Ah;Ahn, Chang Ho;Kim, Yae Jin;Park, Pil Man;An, Hye Ryun;Lee, Ka Youn;Jung, Hyun Hwan
    • Korean Journal of Plant Resources
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    • v.35 no.5
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    • pp.652-658
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    • 2022
  • This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars ('Ice Wing', 'Orange Eye' and 'Pink Beauty') with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 mg/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, 'Ice Wing', which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of 'Ice Wing', which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 mg/L 2,4-D, and 300 mg/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from 'Ice Wing' by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.

Biological Yielding Potential of Rice in Association with Climatic Factors in Yeongnam Region (영남지역 기상과 수도의 한계생산력 해석)

  • Kim, Soon-Chul;Lee, Soo-Kwan;Chung, Geun-Sik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.3
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    • pp.259-270
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    • 1985
  • Meteorological year variations for rice crop from 1973 to 1984 were compared by using air temperature and sunshine hour for nursery period, cooling index for reproductive stage and meteorological yield productivity index for ripening period. The most optimum transplanting date and heading date for crop yield based on real transplanting date-grain yield relationship or heading date-grain yield relationship, meteorological yield productivity index and actual results showed good agreement each other. Around May 26 for transplanting and August 10 for heading were the most optimum date in Indica/Japonica hybrid cultivars while these were about June 8 and August 23 for Japonica cultivars, respectively. On the other hand, theoretical late limiting heading date for safe ripening were August 20 for Indica/Japonica hybrid cultivars and August 30 for Japonica cultivars, respectively, for both methods, cumulative temperature method during ripening with 80% believable frequency and meteorological yield productive index method having 1000(kg/10a) yielding potential. Based on the yield forecast trial, the highest values of photosynthetic efficiency, 2.5%, and crop growth rate, 23g/㎡/day, were recorded during 30 days before rice heading. Considering the photosynthetic efficiency and solar radiation, the potential crop growth rate was more or less 30g/㎡/day and the biological grain yielding potential in a existing cultural practices was approximately 900-1000(kg/10a) in Milyang weather condition. To increase further yielding potential, either photosynthetic efficiency or harvest index or both should be improved by manipulating appropriate canopy architecture, plant spacing, fertilizer, chemical, etc.

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Transition of Rice Culture Practices during Chosun Dynasty through Old References V. Cultivation and Cropping Patterns (주요 고농서를 통한 조선시대의 도작기술 전개 과정 연구 V. 재배양식)

  • Lee, Sung-Kyum;Guh, Ja-Ok;Lee, Eun-Woong;Lee, Hong-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.1
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    • pp.104-115
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    • 1992
  • The rice culture techniques included 'Jodosukyungbeob'(旱稻水耕法 : culture techniques of early-ripening paddy rice), 'Mandosukyungbeob' (晩稻水耕法) : culture techniques of late-Ripening paddy rice 'Handobeob'(旱稻<山稻>法 : culture techniques of upland rice), 'Myojongbeob'(苗種法 : culture techniques of paddy rice by transplanting), 'Kunangbeob'(乾秧法 : culture techniques of rice by transplanting which rears seeding in dry paddy) and 'Sudogunpanongbeob'(水稻乾播農法 : culture techniques of paddy rice seeding in dry field). Especially, 'Kunangbeob' and 'Sudogunpanongbeob' were originally developed in Korea as seen in 1600s(Kyoungje : 經濟) and early 1800s (Yoji : 要旨). In 'Jodosukyungbeob' it took 9 days for seed dipping, water-sprouting and prevent damage by birds, each for 3 days in China, but in Korea seed dipping in water took 3 days and the rest of the procedures were flexibly established. In matured soils, practices were fall plowing right after harvest, recognition of effective tillering and additional fertilization use of human manure, and stimulation of sprouting by lime application. The unique culture techniques adequate for Korean situations were practiced, which included weed control after draining accurately for 3 to 4 times, draining at mid season for improving wind and drought tolerance, rice harvesting at appropriate time for preventing grain shattering, and seeding in rows. 'Mandosukyungbeob' was improved techniques contrast to those of China, and the major contents were selection of proper varieties, good stand establishment by seeding high rates, induction of vigorous tillers, and adoption of 'Jokjongbeob'(足種法 : seeding method by foot). Also, one of the most prominent rice cultures by our ancestors was 'Kunpanongbeob' that was systemized form habitual practice of Pyongan Province. The unique technique actualized was 'Hando [旱稻(山稻)]' culture technique which was the combinations of 'Jokjongbeob', root stimulation method, and disaster-tolerant mixture cropping with adoptation of variety theory, although it was originated from China. The transplanting techniques has come before 'Jikseol'($\ulcorner$直說$\lrcorner$) and its merits were sufficiently realized. However, this method was basically prohibited from the early Chosun dynasty because extremely bad harvest was expected under drought conditions and insufficient conditions of water storage. But, it was permitted in the areas that contained water all the times and in case of large-scale farming especially. Most of rice culture was transplanted in the end of the Chosun dynasty because transplanting was continuously spreaded in the three southern provinces of Korea. Under these circumstances, transplanting technique was improved from the early to the end of the Chosun dynasty by weed control, fertilizing, water management, and quadratic transplanting. Based on these techniques, agricultural productivity was improved 5 times by that time. 'Kunpanongbeob' was created and developed properly for Korean conditions that is dry in early season and flooding in late season. This was successively developed and established into transplanting technique of nursery seedling.

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Development of Precision Overhead Watering and Boom Irrigation System for Fruit Vegetable Seedlings (과채류 육묘용 정밀 두상관수 시스템 개발)

  • Dong Hyeon Kang;Soon Joong Hong;Dong Eok Kim;Min Jung Park
    • Journal of Bio-Environment Control
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    • v.32 no.1
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    • pp.8-14
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    • 2023
  • This study was conducted to develop a precision automatic irrigation system in a nursery by considering the problems and improvements of manual and the conventional automatic irrigation system. The amount of irrigated water between the conventional automatic irrigation system and manual irrigation was 28.7 ± 4.4 g and 14.2 ± 4.3 g, respectively, and the coefficient of variation was less than 30%. However, the coefficient of variation of the conventional automatic irrigation system of 15%, was higher than that of manual irrigation of 30%. The irrigation test using the developed uniform irrigation system attached with the nozzle of a spray angle 80° and most highest uniformity was at height 600 mm. And coefficient of variation of the irrigation uniformity at the center part was within 20%, but irrigation amount of the edge part was lower 50% and over compared to the center part. As a result of a tomato grafting seedling cultivation test using the developed uniform irrigation system, the average plant height of seedling at the edge part was 28 mm but plant height at the center part was higher as 72 mm. Therefore, it was necessary to apply additional irrigation device at the edge part. The irrigation uniformity of the edge concentrated irrigation system was investigated that the irrigation amount of the edge part was irrigated by more than 50% compared with the center part, and coefficient of variation of the irrigation amount at the center part was less than 30%. As a result of a cucumber grafting seedling cultivation test using the edge concentrated irrigation system, the plant height of seedlings in the edge and central part of cultivation bed were 24% and 26%, respectively, so irrigation uniformity was higher then the uniform irrigation system. In order to improve the uniformity of seedlings, it is necessary to adjust the height of boom according to the growth of the seedling by installing a distance sensor in the overhead watering and boom irrigation system.